Translational Medicine最新文献

{"title":"Developed method of three-plane magnetic resonance pelviophetometry.","authors":"E. D. Vyshedkevich, S. R. Sheriev, I. S. Paleeva, I. Mashchenko, A. Y. Efimtsev","doi":"10.18705/2311-4495-2023-10-5-455-465","DOIUrl":"https://doi.org/10.18705/2311-4495-2023-10-5-455-465","url":null,"abstract":"Background. Magnetic resonance pelviophetometry is a highly informative, non-invasive and safe method for assessing the size of the pelvis of a pregnant woman and the fetal head, but it has several disadvantages — a long scanning time, heating of the tissues of the pregnant woman and the fetus, and operator dependence.Objective. Evaluation of the capabilities of the three-plane 3D Dixon MR-pelviophetometry (3D MR-PFM) technique, its comparative analysis with the generally accepted method of two-plane 2D MR-pelviophetometry (2D MR-PFM).Design and methods. A retrospective analysis was perormed out in 26 pregnant women with risk for the development clinically narrow pelvis for various reasons. Results. The data obtained using the Dixon pulse sequence is comparable to the data obtained during the standard technique.Conclusion. The Dixon sequence can be used for the developed 3D method of MR pelviophetometry, since it allows to reduce the scanning time and, according to statistical analysis, show comparable results.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139224773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of the spectrum of biological activity of oxidation products of fluorinated esters of resin acids.","authors":"L. M. Popova, Yu. G. Bazarnova, E. I. Pochkaeva","doi":"10.18705/2311-4495-2023-10-5-377-388","DOIUrl":"https://doi.org/10.18705/2311-4495-2023-10-5-377-388","url":null,"abstract":"Background. Derivatives of diterpenes, in particular, resin acids, are of interest for the creation of pharmacological substances with a wide spectrum of action.Objective. To perform a predictive assessment of the biological activity of oxidation products of fluorinated esters of resin acids.Design and methods. Predictive assessment of the biological activity of the oxidation products of fluorinated esters of resin acids (I–VIII) was carried out using the PASS-online software. The prediction of physico-chemical and pharmacological properties of the studied compounds was carried out using the SWISS-ADME web server and SwissTargetPrediction.Results. A predictive assessment of the spectrum of biological activity, physico-chemical and pharmacological properties of oxidation products of fluorinated esters of resin acids was carried out using PASS-online modeling and the SWISS-ADME and SwissTargetPrediction web server. All the studied compounds (I–VIII) are capable of exhibiting antiviral, antitumor, and dermatological activity. These results indicate the potential of using oxidized fluorinated esters of resin acids as a platform for the creation of pharmacological substances. Using SwissTargetPrediction, it was demonstrated that the most likely target (92 %) for compound (I) is a cholesterol ester transporter protein.Conclusion. Based on the results of a predictive assessment of the spectrum of biological activity using the PASS-online and SWISS-ADME programs, the possibility of using oxidation products of fluorinated esters of resin acids as pharmaceutical substances was revealed.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139220048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tension headache. State of the problem, new aspects of etiopathogenesis, neuroimaging possibilities, non-drug treatment methods (review)","authors":"A. Lepekhina, M. Pospelova, A. Y. Efimtsev, A. Levchuk, G. E. Trufanov, T. Alekseeva, D. V. Piskovatskov","doi":"10.18705/2311-4495-2020-7-2-6-11","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-2-6-11","url":null,"abstract":"The prospects for the use of functional magnetic resonance imaging methods in the diagnosis of cognitive and neuropsychiatric disorders in patients with tension headaches are quite wide, as they allow a more detailed study of the pathogenesis of the above disorders. To date, the effectiveness of osteopathy in the treatment of patients with tension headaches has been shown. It remains an open question to develop a generally accepted method of diagnosis and treatment, including the use of complementary therapies, neuroimaging objectification and determination of the state of functional connections of the brain for more accurate diagnosis and prediction of treatment effectiveness. The present review summarizes the current ideas about the etiopathogenesis of tension headache (including the study of the functional connections of the brain) and available data on the clinical efficacy of osteopathic correction.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141204276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notch-зависимая активация остеогенного потенциала клеток периодонта","authors":"Д. С. Семенова, А. С. Костина, А. М. Мустаева, П. Е. Клаузен, М. А. Добрынин, Н. В. Боярская, Ю. А. Домбровская, Анна Борисовна Малашичева, Н. И. Енукашвили","doi":"10.18705/2311-4495-2020-7-2-21-32","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-2-21-32","url":null,"abstract":"Background and objective . Periodontal stem cells are a promising material for dentistry and maxillofacial surgery. In this work, we sought to find out how Notch signaling pathway affects the osteogenic potential of periodontal ligament stem cells (PDLSC). Design and methods . In order to verify this, we used lentiviral transduction of PDLSC, adding various amounts of the virus bearing the activated intracellular domain of the Notch1 receptor, NICD. Using real-time PCR, we analyzed the dependence of changes in the expression levels of osteo-markers ( RUNX2, COL1A1, OGN, POSTN ) on the activation strength of Notch signaling pathway, based on the expression of the target gene — HEY1 , in the early stages of differentiation, after 48 and 120 hours after induction of osteogenic differentiation. In addition, using the technique of Alizarin red extraction, we were able to quantify the intensity of mineralization of periodontal MSCs at the final stage of osteogenic differentiation. Results . We found that activation of Notch signaling pathway leads to increased expression of osteogenic markers in the early stages of osteogenic differentiation induction, which in turn leads to the development of the final stage of osteogenic differentiation, characterized by the formation of calcification. Moreover, the more intensive the initial level of Notch, the stronger and more effective were the processes of osteogenesis. Conclusion . In this work, we showed that activation of Notch signaling pathway leads to an increase in the osteogenic potential of periodontal PDLSC in a dose-dependent manner and induces in the cells processes associated with the accumulation of calcifications, the intensity of formation of which directly depends on the “strength” of Notch signal. The results obtained in this work convince us that Notch is able to modulate osteogenic differentiation, affecting its effectiveness, apparently due to the strength of the signal that is transmitted to the cells.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141204003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of calcification in aortic degeneration","authors":"D. Kostina, V. Uspensky, D. Semenova, A. Kostina, N. V. Boyarskaya, O. Irtyuga, A. Malashicheva","doi":"10.18705/2311-4495-2020-7-1-6-21","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-1-6-21","url":null,"abstract":"Vascular calcification is a widely-spread pathology with high mortality. It is active bioregulated process that is observed in pathogenesis of different desires, associated with metabolic dysfunction, congenital tissue desires and aging. Signal pathways and transcription factors that are involved in vascular calcification are also takes place in normal osteogenesis and/or vascular development. In the review the main attention is payed to the role of signaling pathways BMP (bone morphogenic protein), Notch, Wnt and to the role of transcription factors BMP2, RUNX2, Msx2 in vascular calcification. Probably, dysfunction of osteogenic signal pathways and transdifferentiation of vascular cells to osteoblast-like cells is a common prosses not only for vascular calcification or mineralization, but is a way of vascular degradation in general. Proosteogenic changes at cellular and molecular level may play role in pathogenesis of a disease without manifestation of vascular mineralization, such as thoracic aortic aneurysm. Ability of vascular cells to change their phenotype to osteophenotype is very likely biologically important ability. Over weakness of calcific signaling pathways activity can also lead to vascular pathology. The aim of the review is to overlook the mechanisms of vascular calcification focusing at the role of signal pathways and vascular cells at this process with particular attention to aortic calcification. Understanding the mechanisms of biological regulation of pro- and antiosteogenic processes in pathology and normal conditions opens new opportunities to influence this prosess in order to correct vascular pathologies.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141223567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Procollagen propeptides and visceral obesity index in patients with myocardial infarction with preserved emission faction and various metabolic phenotype","authors":"A. Osokina, V. Karetnikova, O. Polikutina, Iu S Slepynina, O. Gruzdeva, T. P. Artemova, S. N. Ryzhenkova, T. Pecherina, O. Barbarash","doi":"10.18705/2311-4495-2020-7-1-22-32","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-1-22-32","url":null,"abstract":"Purpose. To identify the peculiarities of the dynamics of fibrosis markers of C-terminal procollagen propeptide type I (PICP) and N-terminal collagen propeptide type III (PIIINP) in patients with ST segment elevation myocardial infarction (STEMI) and preserved myocardial contractility.Material and methods. 86 patients with STEMI and preserved left ventricular ejection fraction were examined. In addition to the standard laboratory and instrumental examinations, all the patients underwent the estimation of the concentrations of C-terminal procollagen propeptide type I (PICP) and N-terminal collagen propeptide type III (PIIINP) by enzyme-linked immunoassay using BCM Diagnostics laboratory kits (USA) on the 1st and the 12th days of disease and 1 year later.Results. The concentration of PICP was significantly reduced in all groups during the entire observation period. Significant correlations were found between PICP and visceral obesity index, age and left ventricular ejection fraction. A relationship was found between PIIINP and an increased visceral obesity index.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141223657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid surgery in treatment of patients with obliterating atherosclerosis of lower extremities (review)","authors":"M. B. Temrezov, V. I. Kovalenko, T. K. Temerezov, A. S. Bakhmetev, V. S. Loyko, M. O. Rudakov","doi":"10.18705/2311-4495-2020-7-1-33-38","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-1-33-38","url":null,"abstract":"The literature review presents the possibilities and current trends in the use of hybrid surgery in patients with multilevel multifocal lesions of the arterial bed of the lower extremities with obliterating lesions. Methods of open bypass surgery for chronic atherosclerosis of the arteries have long been developed, put into practice and can be considered individually for each patient. However, given the frequent complicated atherosclerotic history in patients with multifocal lesions of various arterial pools, most patients can be recommended more gentle endovascular methods of blood flow correction — balloon angioplasty or stenting. But given certain technical and general parameters (prolonged occlusion, severe calcification, kidney disease, an allergic reaction to a contrast drug, etc.), it is not yet possible to use intravascular correction in absolutely all patients, as a result of which the method of simultaneous or phased hybrid surgery seems to be very relevant and can be used even in patients at extremely high risk. The review describes the possible uses of hybrid technology depending on the level of steno-occlusive lesion and the general prolongation of narrowing of the arterial bed. Also considered are some features of the use of simultaneous or multi-stage hybrid operations. On the basis of modern literature data, the advantage of using a combination of open surgeries and endovascular interventions in comparison with any one revascularization method is shown.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141223899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of calcification in aortic degeneration","authors":"D. Kostina, V. Uspensky, D. Semenova, A. Kostina, N. V. Boyarskaya, O. Irtyuga, A. Malashicheva","doi":"10.18705/2311-4495-2020-7-1-6-21","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-1-6-21","url":null,"abstract":"Vascular calcification is a widely-spread pathology with high mortality. It is active bioregulated process that is observed in pathogenesis of different desires, associated with metabolic dysfunction, congenital tissue desires and aging. Signal pathways and transcription factors that are involved in vascular calcification are also takes place in normal osteogenesis and/or vascular development. In the review the main attention is payed to the role of signaling pathways BMP (bone morphogenic protein), Notch, Wnt and to the role of transcription factors BMP2, RUNX2, Msx2 in vascular calcification. Probably, dysfunction of osteogenic signal pathways and transdifferentiation of vascular cells to osteoblast-like cells is a common prosses not only for vascular calcification or mineralization, but is a way of vascular degradation in general. Proosteogenic changes at cellular and molecular level may play role in pathogenesis of a disease without manifestation of vascular mineralization, such as thoracic aortic aneurysm. Ability of vascular cells to change their phenotype to osteophenotype is very likely biologically important ability. Over weakness of calcific signaling pathways activity can also lead to vascular pathology. The aim of the review is to overlook the mechanisms of vascular calcification focusing at the role of signal pathways and vascular cells at this process with particular attention to aortic calcification. Understanding the mechanisms of biological regulation of pro- and antiosteogenic processes in pathology and normal conditions opens new opportunities to influence this prosess in order to correct vascular pathologies.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141223822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid surgery in treatment of patients with obliterating atherosclerosis of lower extremities (review)","authors":"M. B. Temrezov, V. I. Kovalenko, T. K. Temerezov, A. S. Bakhmetev, V. S. Loyko, M. O. Rudakov","doi":"10.18705/2311-4495-2020-7-1-33-38","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-1-33-38","url":null,"abstract":"The literature review presents the possibilities and current trends in the use of hybrid surgery in patients with multilevel multifocal lesions of the arterial bed of the lower extremities with obliterating lesions. Methods of open bypass surgery for chronic atherosclerosis of the arteries have long been developed, put into practice and can be considered individually for each patient. However, given the frequent complicated atherosclerotic history in patients with multifocal lesions of various arterial pools, most patients can be recommended more gentle endovascular methods of blood flow correction — balloon angioplasty or stenting. But given certain technical and general parameters (prolonged occlusion, severe calcification, kidney disease, an allergic reaction to a contrast drug, etc.), it is not yet possible to use intravascular correction in absolutely all patients, as a result of which the method of simultaneous or phased hybrid surgery seems to be very relevant and can be used even in patients at extremely high risk. The review describes the possible uses of hybrid technology depending on the level of steno-occlusive lesion and the general prolongation of narrowing of the arterial bed. Also considered are some features of the use of simultaneous or multi-stage hybrid operations. On the basis of modern literature data, the advantage of using a combination of open surgeries and endovascular interventions in comparison with any one revascularization method is shown.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141223558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Procollagen propeptides and visceral obesity index in patients with myocardial infarction with preserved emission faction and various metabolic phenotype","authors":"A. Osokina, V. Karetnikova, O. Polikutina, Iu S Slepynina, O. Gruzdeva, T. P. Artemova, S. N. Ryzhenkova, T. Pecherina, O. Barbarash","doi":"10.18705/2311-4495-2020-7-1-22-32","DOIUrl":"https://doi.org/10.18705/2311-4495-2020-7-1-22-32","url":null,"abstract":"Purpose. To identify the peculiarities of the dynamics of fibrosis markers of C-terminal procollagen propeptide type I (PICP) and N-terminal collagen propeptide type III (PIIINP) in patients with ST segment elevation myocardial infarction (STEMI) and preserved myocardial contractility.Material and methods. 86 patients with STEMI and preserved left ventricular ejection fraction were examined. In addition to the standard laboratory and instrumental examinations, all the patients underwent the estimation of the concentrations of C-terminal procollagen propeptide type I (PICP) and N-terminal collagen propeptide type III (PIIINP) by enzyme-linked immunoassay using BCM Diagnostics laboratory kits (USA) on the 1st and the 12th days of disease and 1 year later.Results. The concentration of PICP was significantly reduced in all groups during the entire observation period. Significant correlations were found between PICP and visceral obesity index, age and left ventricular ejection fraction. A relationship was found between PIIINP and an increased visceral obesity index.","PeriodicalId":23243,"journal":{"name":"Translational Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141223738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}