2016 International Conference on Systems in Medicine and Biology (ICSMB)最新文献

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Comet assay based detection of SPION induced DNA damage in human lymphocytes 基于彗星法检测SPION诱导的人淋巴细胞DNA损伤

2016 International Conference on Systems in Medicine and Biology (ICSMB) Pub Date : 1900-01-01 DOI: 10.1109/ICSMB.2016.7915095

Swarupa Ghosh, Ilika Ghosh, A. Mukherjee

{"title":"Comet assay based detection of SPION induced DNA damage in human lymphocytes","authors":"Swarupa Ghosh, Ilika Ghosh, A. Mukherjee","doi":"10.1109/ICSMB.2016.7915095","DOIUrl":"https://doi.org/10.1109/ICSMB.2016.7915095","url":null,"abstract":"Superparamagnetic iron oxide nanoparticle (SPION) coated with suitable biocompatible substances have uses in various biomedical fields, particularly in magnetic resonance imaging, tissue engineering, hyperthermia and drug delivery. In this study we have used two newly formulated SPIONs. SPIONs were coated with biodegradable polymer polylactide co glycolide (PLGA) using of the two types of surfactants-didodecyldimethylammoniumbromide (DMAB) and ±-tocopheryl glycol succinate (TPGS) for surface modification, to extend the application potential in the field of nanomedicine. The present study focuses on the evaluation of genotoxicity if any of the two types of formulated SPIONs on human lymphocyte. Human lymphocytes were exposed to SPIONs at 11.2µg/ml concentrations of Fe in each group for 3 h at 37°C. Single-dose toxicity was tested in isolated lymphocytes using MTT assay. Uncoated SPIONs were found highly toxic while the coated ones showed significantly less cell death. In vitro genotoxicity of the formulated SPIONs showed significantly lower %tail DNA than uncoated SPIONs as detected by comet assay in lymphocytes. The results show that SPION induced genotoxicity is completely dependent on its physicochemical properties. Regulation of these properties by using different coatings could decrease toxicity. Type of surface modification primarily governed the amount of DNA damage as detected by Comet assay. The results also indicate that the coatings on the SPION were biocompatible and suitable for in vivo explorations while the free SPION were found completely unsuitable for in vivo administration.","PeriodicalId":231556,"journal":{"name":"2016 International Conference on Systems in Medicine and Biology (ICSMB)","volume":"45 8","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131520656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilized Growth Factor and peptide on indium tin oxide (ITO) scaffold for long-term hepatocyte culture towards developing a hepatotoxicity bioreactor 在氧化铟锡(ITO)支架上固定化生长因子和肽用于肝细胞长期培养的肝毒性生物反应器的研究

2016 International Conference on Systems in Medicine and Biology (ICSMB) Pub Date : 1900-01-01 DOI: 10.1109/ICSMB.2016.7915101

K. Kosaraju, Durga M. Arvapalli, Kandas Womack, C. Zimmerman, T. Shupe, D. Kuila

{"title":"Immobilized Growth Factor and peptide on indium tin oxide (ITO) scaffold for long-term hepatocyte culture towards developing a hepatotoxicity bioreactor","authors":"K. Kosaraju, Durga M. Arvapalli, Kandas Womack, C. Zimmerman, T. Shupe, D. Kuila","doi":"10.1109/ICSMB.2016.7915101","DOIUrl":"https://doi.org/10.1109/ICSMB.2016.7915101","url":null,"abstract":"The overarching goal of this work is to design and develop organic self-assembled monolayer (SAM) based cell culture platforms (SCCPs) to provide an appropriate microenvironment that promotes cell attachment, growth and functionality with the ultimate goal of developing cell-based bioreactors for rapid drug toxicity screening. We describe proliferation of a model cell line, HepG2, and primary rat hepatocytes for culture periods up to 3 days, on a model peptide, Gastrin Releasing Peptide (GRP), and a Growth Factor, Epidermal Growth Factor (EGF), that is covalently coupled to the amine end group of 3-aminopropyl triethoxysilane (APTES)-Self-Assembled Monolayer (SAM) on conducting indium tin oxide (ITO) substrates. The scaffolds were characterized using contact angle and surface-IR techniques. The cells, HepG2 or primary hepatocytes, were cultured on GRP- and EGF-immobilized scaffolds for 24, 48, and 72 hrs. MTT (3-methyltetrazoliumbromide) cell proliferation and Lactose Dehydrogenase (LDH) cytotoxicity assays were performed on HepG2 cells and primary hepatocytes cultures on peptide and growth factor modified scaffolds to evaluate cellular heath and toxicity. Cell proliferation analysis indicated that the HepG2 cells cultured on EGF- and GRP-immobilized substrates showed increased cell viability with time from 24 to 72 hours. The LDH production after 48 hours was reduced in cells cultured on GRP and EGF immobilized surfaces in comparison to the cells cultured on ITO and ITO-APTES substrates. The results overall showed that cell viability increased and cytotoxicity decreased for both HepG2 cells and primary hepatocytes cultured on GRP- and EGF-modified scaffolds. Furthermore, the increase of cell viability with reduced cytotoxicity is extended to 72 hrs with good biocompatibility.","PeriodicalId":231556,"journal":{"name":"2016 International Conference on Systems in Medicine and Biology (ICSMB)","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127733020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EMG signal based knee joint torque estimation 基于肌电信号的膝关节转矩估计

2016 International Conference on Systems in Medicine and Biology (ICSMB) Pub Date : 1900-01-01 DOI: 10.1109/ICSMB.2016.7915117

T. Anwar, Adel Al-Jumaily

{"title":"EMG signal based knee joint torque estimation","authors":"T. Anwar, Adel Al-Jumaily","doi":"10.1109/ICSMB.2016.7915117","DOIUrl":"https://doi.org/10.1109/ICSMB.2016.7915117","url":null,"abstract":"Although Lower Limb Robotic Rehabilitation device exhibit a great prospect in the rehabilitation of impaired limb, yet it has not been widely applied to clinical rehabilitation of the patient with impairment. This is mostly due to insufficient bidirectional information interaction between exoskeleton and patient. The intended action data that can be extracted from surface electromyography (sEMG) signal may include the intended posture, intended torque, intended knee joint angle and intended desired impedance of the patient. Capturing intended knee joint torque from sEMG signal is one of the necessary parameter to achieve a smooth Human Machine Interaction force in a multilayer control mechanism. In this paper, a new technique to estimate Knee joint torque using SVM has been proposed that has used wavelet feature. The estimator is able to estimate required knee joint torque to lift 5kg, 12kg and 19kg weight using extensor and flexor muscles. Based on weight-torque relationship, greater the weight, greater the torque is required to lift the weight. The estimator has classified required torque of 5kg, 12kg and 19kg with accuracy of 98.7296%, 86.0254% and 95.6443% respectively. The estimator can also be used to estimate torque about knee joint at different joint angle.","PeriodicalId":231556,"journal":{"name":"2016 International Conference on Systems in Medicine and Biology (ICSMB)","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121814941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

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