The Open Antimicrobial Agents Journal最新文献

Inhibition of Intracellular Survival of Multi Drug Resistant Clinical Isolates of Mycobacterium tuberculosis in Macrophages by Curcumin 姜黄素对多药耐药结核分枝杆菌临床分离株巨噬细胞内存活的抑制作用

The Open Antimicrobial Agents Journal Pub Date : 2013-11-15 DOI: 10.2174/1876518101304010001

Pramod K. Gupta, S. Kulkarni, Ramakrishna Rajan

引用次数: 13

Antibiotic Treatment of Respiratory Tract Infections in Ambulatory Care in Belgium 比利时门诊呼吸道感染的抗生素治疗

The Open Antimicrobial Agents Journal Pub Date : 2011-12-29 DOI: 10.2174/1876518101103010053

S. Simoens, J. Verhaegen, P. V. Bleyenbergh, W. Peetermans, M. Decramer

{"title":"Antibiotic Treatment of Respiratory Tract Infections in Ambulatory Care in Belgium","authors":"S. Simoens, J. Verhaegen, P. V. Bleyenbergh, W. Peetermans, M. Decramer","doi":"10.2174/1876518101103010053","DOIUrl":"https://doi.org/10.2174/1876518101103010053","url":null,"abstract":"This study aims to analyse antibiotic treatment of respiratory tract infections in ambulatory care in Belgium by: a) mapping antibiotic consumption over time and breaking down antibiotic consumption by infection type; b) discussing antibiotic treatment as recommended by Belgian guidelines; and c) reviewing the current evidence on the cost- effectiveness of antibiotic treatment. IMS Health data showed that the total volume of antibiotic consumption in ambulatory care in Belgium has increased over the years. Antibiotic consumption mainly originated from the use of broad-spectrum penicillins. The volume of fluoroquinolone use remains well controlled. Policy makers need to target the main drivers of inappropriate antibiotic consumption rather than a specific class of antibiotics when they aim to promote better use of antibiotics in ambulatory care. A s-lactam-based therapy for CAP is recommended as first choice in Belgian guidelines and moxifloxacin is advocated for CAP outpatients with comorbid conditions or outpatients in whom infection with atypical pathogens needs to be considered. Because of its high eradicating power against the target organisms and because H. influenzae is the main pathogen to be covered, amoxicillin-clavulanic acid may be a first choice to treat COPD exacerbations, although this choice is subject to debate. Moxifloxacin is recommended in case of IgE-mediated s-lactam allergy or severe intolerance to s-lactam antibiotics for the treatment of COPD exacerbations or for the treatment of upper respiratory tract infections on the rare occasion that antibiotic treatment is warranted. One study supported the cost- effectiveness of first-line treatment of CAP with moxifloxacin in Belgium.","PeriodicalId":22920,"journal":{"name":"The Open Antimicrobial Agents Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76010227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antibiotic Resistance Prevalence and Pattern in Environmental Bacterial Isolates 环境细菌分离株的抗生素耐药性流行及模式

The Open Antimicrobial Agents Journal Pub Date : 2011-12-29 DOI: 10.2174/1876518101103010045

Manisha Debmandal, S. Mandal, N. Pal

{"title":"Antibiotic Resistance Prevalence and Pattern in Environmental Bacterial Isolates","authors":"Manisha Debmandal, S. Mandal, N. Pal","doi":"10.2174/1876518101103010045","DOIUrl":"https://doi.org/10.2174/1876518101103010045","url":null,"abstract":"The present study investigates the prevalence of antibiotic resistance among bacterial isolates from different environmental samples and determines their resistance patterns. Bacteria were isolated from the Ganges water, the intestine of Labeo rohita, soil samples from agricultural land, and clinical samples of urine, pus, and throat swab. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical characteristics. Antibiotic susceptibility of the isolates was tested by disc diffusion and agar dilution method. A total of 87 bacteria belonging to 13 different genera were isolated. The percentages of resistance detected were, Ax: amoxycillin (82.75%), Te: tetracycline (49.42%), Tr: trimethoprim (41.37%), Ch: chloramphenicol (39.08%), Nx: nalidixic acid (22.98%), Ci: ciprofloxacin (24.13%), S: streptomycin (9.19%), G: gentamycin (4.59%) and Ak: amikacin (4.59%). A majority of 57 (65.51%) strains were multi-resistant; 77 (88.5%) were resistant to at least one drug. Determination of resistance pattern revealed that 3 water isolates and 1 clinical isolate belonging to Pseudomonas aeruginosa (n=3) and Proteus vulgaris (n=1) were resistant to all the 9 antibiotics tested; a Proteus mirabilis strain was resistant to all the drugs except G. In the seven-drug-resistant group, Klebsiella aerogenes showed AxChTeNxTSCi-resistance and P. mirabilis strain exhibited AxChTeNxTrGCi resistance pattern. The high prevalence of antibiotic-resistant bacteria harboring diverse resistance traits could represent a potential health risk. The study of antibiotic resistance helps predict future emergence and guide the development of strategies to counteract this resistance. Therefore periodic and comprehensive survey of antibiotic resistance in the environmental bacteria is required.","PeriodicalId":22920,"journal":{"name":"The Open Antimicrobial Agents Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90534903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Pathogenicity for Mice of a Recombinant of Vaccinia Virus and Protection Against Infection with a Lethal Virus 重组痘苗病毒的小鼠致病性及对致死性病毒感染的保护作用

The Open Antimicrobial Agents Journal Pub Date : 2011-11-29 DOI: 10.2174/1876518101103010037

I. Gurt, E. Katz

引用次数: 0

Amphotericin B: A New Look at Cellular Binding 两性霉素B:细胞结合的新视角

The Open Antimicrobial Agents Journal Pub Date : 2011-11-02 DOI: 10.2174/1876518101103010030

J. Cleary, K. Wasan

{"title":"Amphotericin B: A New Look at Cellular Binding","authors":"J. Cleary, K. Wasan","doi":"10.2174/1876518101103010030","DOIUrl":"https://doi.org/10.2174/1876518101103010030","url":null,"abstract":"Objective: The binding characteristics of the polyene antifungal amphotericin B (AmB) are still arguably unclear 50 years after the first human treatment. Our purpose was to investigate the dynamic of polyene cell associated binding using an AmB directed antibody. Methods: Ex vivo murine model and in vitro mammalian cells studies were utilized to assess the binding characteristics of AmB at human therapeutic concentrations. Balb/c mice administered a single dose of an AmB formulation were assessed using immunohistochemistry. Human mononuculear cells isolated after phlebotomy were assessed using transmission electron micrography. Results & Conclusions: Ex vivo studies demonstrated diffuse binding of AmB formulations, however, the sensitivity of the technique would not allow delineation of the antifungals' cellular binding. However, transmission electron micrography of in vitro cell cultures allowed apparent discrimination of cell associated binding and suggestions of intracellular trafficking within 2 hours of polyene exposure. One could hypothesize the observed characteristics and previously published literature are suggestive of binding to clathrin type receptors with internalization and processing by endosomes. Further work will be required to substantiate this hypothesis.","PeriodicalId":22920,"journal":{"name":"The Open Antimicrobial Agents Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74447311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Conjugated Eicosapentaenoic Acid (cEPA) Inhibits L. donovani TopoisomeraseI and has an Antiproliferative Activity Against L. donovani Promastigotes 共轭二十碳五烯酸(cEPA)抑制L. donovani拓扑异构酶i并对L. donovani Promastigotes具有抗增殖活性

The Open Antimicrobial Agents Journal Pub Date : 2011-09-28 DOI: 10.2174/1876518101103010023

O. Vassallo, S. Castelli, A. Biswas, S. Sengupta, P. Das, I. d’Annessa, F. Oteri, A. Leoni, P. Tagliatesta, H. Majumder, A. Desideri

引用次数: 7

Development of New Vaccine－New Method to Prepare Artificial Membrane Vaccine using Inter-Membrane Protein Transfer 新疫苗的研制——膜间蛋白转移制备人工膜疫苗的新方法

The Open Antimicrobial Agents Journal Pub Date : 2011-09-08 DOI: 10.2174/1876518101103010017

M. Ueno, K. Hayashi

{"title":"Development of New Vaccine－New Method to Prepare Artificial Membrane Vaccine using Inter-Membrane Protein Transfer","authors":"M. Ueno, K. Hayashi","doi":"10.2174/1876518101103010017","DOIUrl":"https://doi.org/10.2174/1876518101103010017","url":null,"abstract":"Influenza is one of the common infectious diseases caused by influenza virus. Vaccines are the most effective means to fight infectious diseases. While these vaccines are quite effective in young adults, however, they are less for the above high risk group. A currently used influenza vaccine contains aluminum hydroxide as an adjuvant, which sometimes induces the production of IgE antibodies causing an allergic reaction. In order to overcome the drawbacks, artificial membrane vaccines or liposome vaccines have been developed. Liposomes are known to be effective immunoadjuvants. On the other hand, various membrane proteins, including matrix membrane proteins, have been reported to be spontaneously transferrable from living cell membranes to artificial membranes (liposomes). In this article, we introduced a new method to prepare the artificial membrane vaccine, \"influenza virosomes\", using inter-membrane protein transfer, and discussed the immunreactivity of the virosomes. We concluded that the application of inter-membrane protein transfer technique is a useful method for the preparation of the artificial membrane vaccine, virosomes. The virosomes showed high immunoreactivity especially with MDP (muramyldipeptide) derivatives as an adjuvant or booster treatment.","PeriodicalId":22920,"journal":{"name":"The Open Antimicrobial Agents Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87860247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In Vitro Antimicrobial and Antibiofilm Activity of DispersinB ® -Triclosan Wound Gel against Chronic Wound-associated Bacteria 分散b®-三氯生伤口凝胶对慢性伤口相关细菌的体外抗菌和抗菌膜活性研究

The Open Antimicrobial Agents Journal Pub Date : 2011-08-25 DOI: 10.2174/1876518101103010012

P. V. Gawande, N. Yakandawala, K. LoVetri, Srinivasa Madhyastha

{"title":"In Vitro Antimicrobial and Antibiofilm Activity of DispersinB ® -Triclosan Wound Gel against Chronic Wound-associated Bacteria","authors":"P. V. Gawande, N. Yakandawala, K. LoVetri, Srinivasa Madhyastha","doi":"10.2174/1876518101103010012","DOIUrl":"https://doi.org/10.2174/1876518101103010012","url":null,"abstract":"Since the traditional methods of treatment have proven ineffective against chronic wounds involving biofilms, the present study evaluates the in vitro efficacy of a novel wound gel comprising an antibiofilm DispersinB ® enzyme and a broad-spectrum antimicrobial triclosan against chronic wound-associated bacteria. The antimicrobial and antibiofilm activity of DispersinB ® -triclosan wound gel was tested against chronic wound-associated bacteria such as Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Staphylococcus epidermidis, coagulase-negative Staphylococci, Acinetobacter baumannii and Klebsiella pneumoniae using a quantitative culture method and a biofilm assay. The DispersinB ® -triclosan wound gel showed more enduring antimicrobial activity against the test organisms compared to commercial wound gels Silver-Sept™ and IODOSORB. When DispersinB ® -triclosan wound gel was tested against preformed biofilm, it showed 2-4 log10 reduction in the biofilm embedded bacterial cells. Furthermore, DispersinB ® - triclosan wound gel compared to IODOSORB was significantly more effective in inhibiting biofilm in MRSA and A. baumannii (P < 0.05). This study indicates the potential application of a broad-spectrum DispersinB ® -triclosan wound gel","PeriodicalId":22920,"journal":{"name":"The Open Antimicrobial Agents Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81616720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Lysostaphin Reduces the Production of Inflammatory Cytokines in Staphylococcus aureus Challenged Mice, and Prevents Systemic Shock 溶葡萄球菌蛋白减少金黄色葡萄球菌感染小鼠炎症细胞因子的产生，并预防全身休克

The Open Antimicrobial Agents Journal Pub Date : 2011-08-15 DOI: 10.2174/1876518101103010006

Clara J. Sei, T. Chanturiya, J. Mond, J. Kokai-Kun

{"title":"Lysostaphin Reduces the Production of Inflammatory Cytokines in Staphylococcus aureus Challenged Mice, and Prevents Systemic Shock","authors":"Clara J. Sei, T. Chanturiya, J. Mond, J. Kokai-Kun","doi":"10.2174/1876518101103010006","DOIUrl":"https://doi.org/10.2174/1876518101103010006","url":null,"abstract":"Lysostaphin is being developed as a treatment for serious staphylococcal infections. Mice challenged with S. aureus produce inflammatory cytokines including, TNF-� and IL-6, and over-production of these cytokines can lead to shock and contribute to the lethality of staphylococcal infections. Two major components of the staphylococcal cell wall, peptidoglycan and lipoteichoic acid, are known to synergize to induce shock and organ failure in animal models, and we wished to determine whether the rapid lysostaphin-mediated degradation of peptidoglycan during treatment of systemic S. aureus infection could affect shock-associated parameters. We found that lysostaphin treatment of S. aureus-infected mice, which reduces bacteremia and organ infection, also reduced the serum levels of inflammatory cytokines and reversed the symptoms of S. aureus-induced shock. We compared the cytokine response of mice challenged with S. aureus to that of mice challenged with S. aureus and then treated with lysostaphin or nafcillin. Lysostaphin-treated mice, as compared with untreated mice or nafcillin-treated mice, had a blunted cytokine responses to S. aureus challenge. Core body temperature was used as a real time indicator for systemic shock in mice. Mice infected with S. aureus demonstrated a rapid drop in core body temperature, which was reversed by lysostaphin treatment. These studies demonstrated that lysostaphin treatment did not contribute to the induction of shock by rapidly releasing staphylococcal cell wall components, but rather it blunted the inflammatory cytokine response and ameliorated shock related symptoms. Keyword: S. aureus, lysostaphin, shock, cytokine release.","PeriodicalId":22920,"journal":{"name":"The Open Antimicrobial Agents Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78283424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Trends in Macrolide Resistance for Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus pneumoniae and its Association with Social Clustering in Argentina 阿根廷化脓性链球菌、无乳链球菌和肺炎链球菌大环内酯类耐药趋势及其与社会聚集性的关系

The Open Antimicrobial Agents Journal Pub Date : 2011-03-30 DOI: 10.2174/1876518101103010001

G. Rubinstein, B. Bavdaz, S. Bunder, N. Blázquez

{"title":"Trends in Macrolide Resistance for Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus pneumoniae and its Association with Social Clustering in Argentina","authors":"G. Rubinstein, B. Bavdaz, S. Bunder, N. Blázquez","doi":"10.2174/1876518101103010001","DOIUrl":"https://doi.org/10.2174/1876518101103010001","url":null,"abstract":"The aim of this work was to study and compare macrolide resistance patterns among Streptococcus pneumoniae, Streptococcus agalactiae and Streptococcus pyogenes, to describe the phenotypes of macrolide-lincosamide resistance and to further investigate associations between macrolide resistance and social clustering. Susceptibility data were obtained from 4 clinical microbiology laboratories in Bariloche, Argentina for the period 2002- 2008. Patients were differentiated in two population clusters according to the institution they attended (public or private). A total of 4310 strains were studied: 2615 S. pyogenes, 995 S. agalactiae and 700 isolates of S.pneumoniae. Erythromycin resistance rates over the study period were 1.5% for S. pyogenes, 7.8% for S. agalactiae and 11.3% for S. pneumoniae. For the complete study group, these values differed significantly among species (contingency table ￻ 2 = 164.52, p<0.0001). Resistance in S.pyogenes and S. pneumoniae showed irregular trends whilst S. agalactiae showed an increasing tendency during the whole period appearing to be a better indicator of the trends in macrolide resistance. A dual character MLSB phenotype of both inducible and constitutive resistance was observed in S. agalactiae only. Resistance in isolates from the population attending private institutions was significantly higher than those attending the public hospital (S. pyogenes, (1.9 vs. 0.6, ￻ 2 = 5.851, p<0.025); S. pneumoniae, 19.1 vs. 7.0 (￻ 2 = 21.98, p<0.001), and. S. agalactiae, 12.4 vs. 3.8 (￻ 2 = 24.5, p<0.001)). In conclusion, macrolides resistance rates during 2002-2008 were significantly different for S. pyogenes, S. agalactiae and S.pneumoniae. We also found significant variations in macrolide resistance levels in different population groups.","PeriodicalId":22920,"journal":{"name":"The Open Antimicrobial Agents Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89307336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1