The Journal of protozoology最新文献_第5页

Restriction fragment length polymorphism analysis of Cryptosporidium parvum isolates of bovine and human origin. 牛源和人源小隐孢子虫分离株限制性内切片段长度多态性分析。

The Journal of protozoology Pub Date : 1991-11-01

Y R Ortega, R R Sheehy, V A Cama, K K Oishi, C R Sterling

{"title":"Restriction fragment length polymorphism analysis of Cryptosporidium parvum isolates of bovine and human origin.","authors":"Y R Ortega, R R Sheehy, V A Cama, K K Oishi, C R Sterling","doi":"","DOIUrl":"","url":null,"abstract":"Cryptosporidium parvum oocysts isolated from different hosts and geographical areas were compared by restriction endonuclease analysis of repetitive DNA: Iowa (bovine), Florida (bovine), New York (bovine), Peru (human), Brazil (human), and Mexico (human). Southern blot hybridization analysis was performed using the restriction endonuclease enzyme Eco RI and the DNA probe pV47-2. The probe hybridized with 18 bands present in all the isolates. The Brazilian, Mexican, and Peruvian human isolates had an additional common band of 4.3 kbp that was absent in the bovine isolates. Two extra bands of 14 and 12 kbp were present in the Brazilian isolate whereas the Mexican isolate had an extra band of 14 kbp. When the Iowa and Peru C. parvum isolates were passed twice through calves, oocysts recovered from both passages showed identical banding patterns, suggesting that recombination of the repetitive sequences was not altered during sexual reproduction. The DNA digested with other restriction endonucleases were tested confirming differences between isolates. A genomic DNA library is currently being produced to better define isolate variation in C. parvum.","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":"38 6","pages":"40S-41S"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12852935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of carbohydrates and lectins on cryptosporidial sporozoite penetration of cultured cell monolayers. 碳水化合物和凝集素对培养细胞单层隐孢子虫孢子子渗透的影响。

The Journal of protozoology Pub Date : 1991-11-01

T L Kuhls, D A Mosier, D L Crawford

{"title":"Effects of carbohydrates and lectins on cryptosporidial sporozoite penetration of cultured cell monolayers.","authors":"T L Kuhls, D A Mosier, D L Crawford","doi":"","DOIUrl":"","url":null,"abstract":"Cryptosporidium parvum first interacts with enterocytes when sporozoites penetrate the host plasma membrane. We have developed a shell vial assay using human embryonic Intestine 407 cells and purified C. parvum sporozoites to study this process. Sporozoites were incubated in culture medium with various carbohydrates and lectins, and the suspensions were then added to the cell monolayers. Following incubation, the monolayers were fixed and stained and the number of schizonts were counted. No decreases in sporozoite motility or Intestine 407 cell viability were observed with carbohydrate or lectin treatment. N-Acetyl-D-glucosamine, chitobiose and chitotriose inhibited C. parvum infection, compared to 5 other tested carbohydrates. Wheat germ agglutinin reduced penetration and concanavalin A enhanced schizont formation, when compared to 8 other lectins. Next, we pretreated sporozoites or Intestine 407 cells with wheat germ agglutinin and concanaval in A prior to sporozoite inoculation. Wheat germ agglutinin treatment of sporozoites or cells equally caused a reduction in C. parvum infection, while enhancement was only observed when Intestine 407 cell were pretreated with concanavalin A. These data suggest that glycoproteins with terminal N-acetyl-D-glucosamine residues may play a role in C. parvum adhesion or penetration of enterocytes. Also, host glycoproteins with concanavalin A-like activity may play a role in these processes.","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":"38 6","pages":"74S-76S"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture, electron microscopy, and immunoblot studies on a microsporidian parasite isolated from the urine of a patient with AIDS. 从艾滋病患者尿液中分离的微孢子虫的培养、电镜和免疫印迹研究。

The Journal of protozoology Pub Date : 1991-11-01

G S Visvesvara, G J Leitch, H Moura, S Wallace, R Weber, R T Bryan

{"title":"Culture, electron microscopy, and immunoblot studies on a microsporidian parasite isolated from the urine of a patient with AIDS.","authors":"G S Visvesvara, G J Leitch, H Moura, S Wallace, R Weber, R T Bryan","doi":"","DOIUrl":"","url":null,"abstract":"Microsporidian spores isolated from a urine sample of an HIV-positive patient were inoculated onto monolayers of six different cell cultures. The parasites (CDC:0291:V213) grew profusely in two of the cultures (HLF and E6) and extruded spores into the culture medium. The spores were Gram-positive, 2.25- to 2.8-microns long, 1.25- to 1.8-microns broad, and smooth-walled. Some of the spores had already extruded their polar tubes, which were either straight or slightly coiled. Infected host cells contained parasitophorous vacuoles filled with developing stages of the parasite, including mature spores. Each spore was surrounded by a thin, electron-dense exospore; a thick electron-lucent endospore; and a thin cell membrane. Cross-sections of six coils of the polar tube were seen inside the spore. Proteins extracted from spores of our isolate and those from Encephalitozoon cuniculi were separated on gradient sodium dodecyl sulfate-polyacrylamide gels and either silver-stained or transferred to nitrocellulose membranes. As many as 35 bands, ranging in molecular mass from 10,000 to 200,000, were visualized in the silver-stained gel. When reacted with the serum of our patient, strips cut from the membrane showed a number of bands ranging in molecular weight from 25,000 to 200,000. However, unique differences between the profiles of the two parasites were seen both in the immunoblot and the silver-stained protein profiles. Based on these findings, we conclude that our isolate belongs to the genus Encephalitozoon, but more studies are needed to identify our isolate to the species level.","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":"38 6","pages":"105S-111S"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-cryptosporidial activity of macrolides in immunosuppressed rats. 免疫抑制大鼠大环内酯类抗隐孢子虫活性。

The Journal of protozoology Pub Date : 1991-11-01

J E Rehg

引用次数: 0

Intestinal injury associated with spiramycin therapy of Cryptosporidium infection in AIDS. 螺旋霉素治疗艾滋病隐孢子虫感染的肠道损伤。

The Journal of protozoology Pub Date : 1991-11-01

C Weikel, A Lazenby, P Belitsos, M McDewitt, H E Fleming, M Barbacci

引用次数: 0

Immunotherapy of cryptosporidiosis in immunodeficient animal models. 免疫缺陷动物模型隐孢子虫病的免疫治疗。

The Journal of protozoology Pub Date : 1991-11-01

L E Perryman, J M Bjorneby

{"title":"Immunotherapy of cryptosporidiosis in immunodeficient animal models.","authors":"L E Perryman, J M Bjorneby","doi":"","DOIUrl":"","url":null,"abstract":"Immunotherapy for persistent infection caused by Cryptosporidium parvum was attempted in two immunodeficient animal models. BALB/c Athymic (nude) mice were infected with two oral doses of 2 x 10(7) C. parvum oocysts, and subsequently treated with monoclonal antibody (MAb) 17.41 that neutralizes sporozoites and merozoites. Persistent infection was established in all exposed mice. Daily oral treatment with MAb 17.41 for 10 days significantly reduced (p less than 0.005) the number of C. parvum organisms observed by microscopic study of intestinal tracts of infected mice. Young horses with severe combined immunodeficiency (SCID) also developed persistent infection following oral exposure with 10(8) C. parvum oocysts. In contrast to nude mice, SCID foals exhibited diarrhea associated with oocyst shedding. Two foals were treated orally with MAb 18.44 and immune serum, both of which neutralized C. parvum sporozoites and merozoites. Oocyst shedding patterns did not significantly differ from those in five SCID foals treated with nonimmune reagents. The results obtained indicate that SCID foals are a useful large animal model of clinical disease associated with persistent C. parvum infection, and that nude mice are a convenient animal model for testing therapeutic potential of antibodies in persistent cryptosporidial infection.","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":"38 6","pages":"98S-100S"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12978998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of Cryptosporidium muris-like oocysts among cattle populations of the United States: preliminary report. 美国牛群中隐孢子虫样卵囊的流行:初步报告。

The Journal of protozoology Pub Date : 1991-11-01

B C Anderson

引用次数: 0

Cryptosporidium merozoite isolation and purification using differential centrifugation techniques. 差动离心技术分离纯化隐孢子虫胚。

The Journal of protozoology Pub Date : 1991-11-01

S Regan, V Cama, C R Sterling

{"title":"Cryptosporidium merozoite isolation and purification using differential centrifugation techniques.","authors":"S Regan, V Cama, C R Sterling","doi":"","DOIUrl":"","url":null,"abstract":"Simple modifications to a recently published merozoite purification procedure (Bjorneby et al., J. Immunol. 145:298, 1990) increased yields 3- to 5-fold. Calves were infected with 2.5 x 10(8) Cryptosporidium parvum oocysts and sacrificed 65 h post-infection. The ilium and caecum were removed. The tissue was sieved through a large strainer (2 mm2) to produce a homogeneous suspension. Red blood cells were removed by differential centrifugation (600 g); merozoites remained in the supernatant. The merozoites were pelleted (2,100 g) and washed in modified Hank's balanced salt solution deficient in Mg+2 and Ca+2. Percoll purification (density 1.070 g/ml and centrifugation speed of 22,000 g for 30 min) yielded 8 x 10(8) merozoites. Nineteen monoclonal antibodies (MAb) detected by either an enzyme-linked immunosorbent assay or an immunofluorescence assay, have been generated against the merozoite stage. Gels of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver-stained showed that sporozoites and merozoites have many common lower molecular weight proteins. Western blots of sporozoite and merozoite antigens reacted with anti-sporozoite MAb showed several cross-reacting antigens shared by these life-cycle stages.","PeriodicalId":22758,"journal":{"name":"The Journal of protozoology","volume":"38 6","pages":"202S-204S"},"PeriodicalIF":0.0,"publicationDate":"1991-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12979584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidial infections in SCID mice reconstituted with human or murine lymphocytes. 用人或鼠淋巴细胞重建SCID小鼠隐孢子虫感染。

The Journal of protozoology Pub Date : 1991-11-01

J R Mead, M J Arrowood, M C Healey, R W Sidwell

引用次数: 0

Isolation and characterization of rat lung Pneumocystis carinii gp120. 大鼠肺卡氏肺囊虫gp120的分离与鉴定。

The Journal of protozoology Pub Date : 1991-11-01

J R Paulsrud, S F Queener, M S Bartlett, J W Smith

引用次数: 0