The Korean Journal of Crop Science最新文献

{"title":"Development of Real-time Quantitative PCR Assay based on SYBR Green I and TaqMan Probe for Detection of Apple Viruses","authors":"Seong Heo and Yong Suk Chung","doi":"10.7740/KJCS.2020.65.4.496","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.4.496","url":null,"abstract":"Virus infections of apples result in lowered commercial qualities such as low sugar content, weakened tree vigor, and malformed fruits. An effective way to control viruses is to produce virus-free plants based on the development of an accurate and sensitive diagnostic method. In this study, real-time PCR assays based on SYBR Green I and TaqMan probes were developed for detecting ASGV, ASPV, and ApMV viruses. These methods can detect and quantify 103 to 1011 RNA copies/μL of each virus separately. Compared with methods with two different dyes, the SYBR Green I-based method was efficient for virus detection as well as for assay using the TaqMan probe. Field tests demonstrated that real-time PCR methods developed in this study were applicable to high-throughput diagnoses for virus research and plant quarantine.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"7 1","pages":"496-507"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84946201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of LED Light Intensity on Seedling Quality and Tuber Production of Potato Stem Cuttings Grown in a Closed-Type Plant Production System","authors":"M. Jo, I. Ham, K. Park, Ji-Hong Cho","doi":"10.7740/KJCS.2020.65.4.468","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.4.468","url":null,"abstract":"This study was performed to establish light intensity conditions for producing stem cuttings for aeroponic systems suitable for seed potato production using a closed-type plant production system. Shoot tip cultured plantlets of ‘Sumi’ and ‘Chubaek’ potato (Solanum tuberosum L.) were acclimatized, cuttings were collected, and stem cuttings were planted. The seedlings were raised for 40 days at different LED light intensities (60, 120, 180, and 240 μmol·m-2·s-1), and were cultivated in an aeroponic system for 80 days. When stem cuttings were raised at 60 μmol·m-2·s-1 LED light intensity, the plant height was the longest, at 17.3 cm for ‘Sumi’ and 16.1 cm for ‘Chubaek’, and the number of nodes was the highest in both cultivars. The higher light intensities, produced smaller plants with fewer nodes. The leaf areas, SPAD values, and Fv/Fm values differed slightly between cultivars. The fresh weight of stem cuttings, and the production rate of healthy stem cuttings were the highest at 60 μmol·m-2·s-1. In the aeroponic system, seedlings raised at 60 μmol·m-2·s-1 with LED light intensity showed a difference between the cultivars, but the fresh weight of stems and leaves above the planting plate was the heaviest. In addition, below the planting plate the stem cuttings were longest and the root weight was heaviest at 60 μmol·m-2·s-1 LED light intensity. The number of stolons also differed between cultivars, but was greatest for seedlings raised at 60 μmol·m-2·s-1 LED light intensity, at 4.2/plant for ‘Sumi’ and 7.7/plant for ‘Chubaek’. At 60 μmol·m-2·s-1 LED light intensity, the tuber number and total tuber weight were the best, but the higher the light intensity, the smaller the total tuber number and total tuber weight for both cultivars. In conclusion, when producing potato stem cuttings for aeroponic systems using a closed-type plant production system, the most suitable LED light intensity for raising seedlings was found to be 60 μmol·m-2·s-1.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"84 1","pages":"468-476"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90636520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Productivity of Early Maturity Silage Corns during Continuous Monocropping","authors":"B. Son, Hwan-Hee Bae, Y. Go, Sun-Lim Kim, Seonghyu Shin","doi":"10.7740/KJCS.2020.65.4.416","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.4.416","url":null,"abstract":"This study evaluated the productivity of early maturity silage corns during continuous monocropping and the possibility of expanding forage production. Continuous monocropping of three silage corns, such as Kwangpyeongok (medium maturity), Sinhwangok (early maturity), and Sinhwangok2 (early maturity) was planted twice, in April and July from 2018 to 2019 at Suwon. The number of days from the sowing date to the silking date was 78 for the preceding crop and 52 for the succeeding crop. The number of days from the silking date to the harvesting date was 26 for the preceding crop and 46 for the succeeding crop. The sums of the temperature from the sowing date to the silking date were 1,512°C for the preceding crop, 1,246°C for the succeeding crop. The sums of the temperature from the sowing date to the harvesting date were 2,198°C for the preceding crop and 1,951°C for the succeeding crop. The dry matter yield of the preceding crop (1,637 kg/10a) was similar to that of the succeeding crop (1,565 kg/10a). The dry matter yields of Sinhwangok2 (1,673 kg/10a), Sinhwangok (1,660 kg/10a) and Kwangpyeongok (1,579 kg/10a) were similar to those of the preceding crop. The dry matter yields of Sinhwangok (1,669 kg/10a) and Kwangpyeongok (1,651 kg/10a) were similar to those of the succeeding crop and Sinhwangok2 (1,374 kg/10a) was the lowest among the three corn varieties. The total digestible nutrients (TDN) yield of the preceding crop (1,135 kg/10a) was similar to that of the succeeding crop (1,037 kg/10a). The TDN yields of Sinhwangok2 (1,183 kg/10a), Sinhwangok (1,158 kg/10a), and Kwangpyeongok (1,063 kg/10a) were similar to those of the preceding crop. The TDN yields of Sinhwangok (1,150 kg/10a) and Kwangpyeongok (1,100 kg/10a) were similar for the succeeding crop and Sinhwangok2 (970 kg/10a) was the lowest among the three corn varieties. The total dry matter yields of Sinhwangok (3,329 kg/10a) and Kwangpyeongok (3,230 kg/10a) were similar, but Sinhwangok2 (3,047 kg/10a) was the lowest among the three corn varieties. The total TDN yields of Sinhwangok (2,307 kg/10a), Kwangpyeongok (2,162 kg/10a), and Sinhwangok2 (2,152 kg/10a) were similar. It was concluded that Sinhwangok and Sinhwangok2 have high TDN yields as well as early maturity, and therefore are advantageous for direct continuous monocropping.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"27 1","pages":"416-425"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81979610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Zebularine Soaking on the Early Growth Stage and Mitotic Chromosomes of Barley (Hordeum vulgare L.)","authors":"Ji-yoon Han, Seong-Wook Kang, Jae-Buhm Chun, Yang-Kil Kim, Young-mi Yoon, Seong-Woo Cho","doi":"10.7740/KJCS.2020.65.4.399","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.4.399","url":null,"abstract":"The objective of this study was to identify the effect of zebularine soaking on the early growth stage of barley (Hordeum vulgare L.). Hence, root elongation was measured daily according to the different concentrations of zebularine (1.0, 2.5, 5.0, and 10.0 μM) for 4 days. On the first day, root length at 2.5 and 5.0 μM was significantly longer than that in the non-treated control. On the second day, root length was not significantly different among all concentrations and controls. On the third day, root elongation was suppressed by the effect of zebularine, except at 2.5 μM. The treatment time of zebularine accounted for the largest proportion of the variation in root elongation. After transplanting, plant growth velocity was similar to that of the control; however, plants at 2.5 μM showed faster growth velocity than that of the other concentrations and the control. In the metaphase of mitosis, most chromosomes of cells under zebularine treatment were ordinary regardless of the concentration, while some cells with short chromosomes were investigated at around 2%. The short chromosome showed a centromere. In addition, it showed short and long arms based on the centromere. The lengths of the short and long arms were different for each short chromosome. It is necessary to study the effect of the short chromosome as a chromosomal function on plant growth and phenotype through investigation of meiosis and fertilization at the chromosome level.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"1 1","pages":"399-405"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80974521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Five Forage Rice Cultivars for Salinity Tolerance at the Seedling Stage and Cultural Practice in a Double-cropping Reclaimed Paddy","authors":"Pil-Mo Sung, Sun-Woong Yun, Eun-Ji Song, Su-Hwan Lee, Jin-Woong Cho, N. Chung","doi":"10.7740/KJCS.2020.65.4.255","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.4.255","url":null,"abstract":"In this study, we evaluated the salinity tolerance of five forage rice cultivars (Mogwoo, Mogyang, Miwoo, Jowoo, and Yeongwoo) with respect to germination, seedling growth, productivity, and feed values in a reclaimed paddy field in Muan, Korea. We found that the cultivars Mogwoo, Miwoo, Jowoo, and Yeongwoo showed normal germination and seedling growth, even at saline concentrations of between 0.1% and 0.5%, whereas in contrast, there were significant reductions in the germination and seedling growth of Mokyang with an increase in salt concentration. Among the five cultivars assessed, the seedling growth of Mogwoo and Yeongwoo was relatively higher at different salt concentrations in hydroponic and sand cultivation. Furthermore, cultivation of the five cultivars in the reclaimed paddy field with an initial salt concentration of 0.3% revealed that Yeongwoo was relatively superior in terms of yield and feed value. In conclusion, the germination and early growth of Yeongwoo were relatively good at high salt concentrations, and the yield and forage value of this cultivar in a reclaimed paddy field were also relatively superior to those of the other assessed varieties.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"61 1","pages":"255-263"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91209083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Climate on the Yield of ‘Ilpum’ Rice Cultivar in Gyeongbuk Province, South Korea over the Past 25 Years","authors":"Jongho Shin","doi":"10.7740/KJCS.2020.65.4.264","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.4.264","url":null,"abstract":"Ilpum’, a Korean short-grain mid-late maturing rice cultivar, was developed in 1990, more than 30 years ago. Despite its age, it has been the most widely grown cultivar in the Gyeongbuk province of South Korea for more than 25 years, making it the most important rice cultivar for the people of the Gyeongbuk province. The aim of this study was to analyze the relationship between the rice yield of “Ilpum’, the main rice cultivar in the Gyeongbuk province, and climate elements in the Daegu (southern plain area) and the Andong (inland mountainous area) regions in Gyeongbuk, South Korea. The rice yield over the past five years increased by about 13% and 24%, compared to that produced in the late 1990s in Daegu and the early 2000s in the Andong region, respectively. The number of panicles per hill and the grain ripening rate significantly affected rice yield in the ‘Ilpum’ cultivars in the Daegu region. The faster heading was a factor in the increase in ‘Ilpum’ rice yield in the Andong region. The air temperature has been rising and sunshine duration has been increasing from the late 1990s to present in both regions. Rice yield was evaluated to understand the effect of climate factors. The rice yields increased owing to the long sunshine duration during the grain-filling stage in both regions. In Andong, increasing the maximum temperature during the vegetative stage increased rice yield. Rising air temperature during the reproductive stage also increased rice yield. In particular, long sunshine hours throughout the whole rice growing period increased the rice yield of this cultivar in the Andong region.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"50 1","pages":"264-273"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83285426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Harvest Time on Yield Components and Quality in ‘Heuksaeng’, the Purple-Skin Peanut Variety","authors":"Eunyoung Oh, S. Pae, J. E. Park, Jung-In Kim, Sungup Kim, M. Lee, T. Ha","doi":"10.7740/KJCS.2020.65.3.248","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.3.248","url":null,"abstract":"","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"30 1","pages":"248-254"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90843556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of Salt-Tolerant Silage Rice Through in vitro Screening and Saltol QTL Analysis","authors":"Chuloh Cho, Kyung Hwa Kim, E. Ahn, Hyang-Mi Park, M. Choi, Jae-Buhm Chun, Mi-Suk Seo, M. Jin, Dool-Yi Kim","doi":"10.7740/KJCS.2020.65.3.214","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.3.214","url":null,"abstract":"Salinity is one of the major abiotic stressors that inhibits the growth, yield, and productivity of crop plants. Therefore, it is necessary to develop crops with increased salt tolerance for cultivation in saline soils such as is found in reclaimed land. The objective of this study was to develop a salt-tolerant silage rice line that grows on reclaimed land. In order to develop this salt-tolerant silage rice, we transferred Saltol, a major QTL associated with salt tolerance, from IR64-Saltol, a salt-tolerant indica variety, into Mogyang, a susceptible elite japonica variety. To determine the effect of salt stress, Mogyang and IR64-Saltol cultivars were grown on a medium containing various concentrations of NaCl in in vitro conditions. Shoot length was found to decrease with increasing salt concentrations, and root growth was almost arrested at NaCl concentrations over 50 mM in the Mogyang cultivar. Based on these preliminary results, we screened five salt-tolerant lines showing superior growth under salt stress conditions. Polymerase chain reaction and sequencing results showed that the introgression types of Saltol QTL were derived from the IR64-Saltol cultivar in almost all selected lines. Based on the observed growth and physiological characteristics, the new Saltol introgression lines showed higher salt tolerance compared to the Mogyang parental cultivar. The salt-tolerant lines identified in this study could be used as a genetic resource to improve rice salt tolerance.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"5 1","pages":"214-221"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83481572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and Agronomic Analysis of a Recombinant Inbred Line Population to Map Quantitative Trait Loci for Blast Resistance and Select Promising Lines in Rice","authors":"Su-Kyung Ha, J. Jeung, Jong‐Min Jeong, Jinhee Kim, Y. Mo","doi":"10.7740/KJCS.2020.65.3.172","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.3.172","url":null,"abstract":"Koshihikari has been one of the most popular rice cultivars with good eating quality since the 1960s despite its susceptibility to blast disease and lodging. To map the genes controlling blast resistance and to develop promising blast-resistant breeding lines inheriting Koshihikari’s high eating quality, a recombinant inbred line (RIL) population was developed from a cross between Koshihikari and a blast resistance donor with early maturity, Baegilmi. A total of 394 Koshihikari × Baegilmi RILs (KBRIL), and the two parents, were evaluated for blast resistance and major agronomic traits including heading date, culm length, panicle length, and tiller number. A linkage map encompassing 1,272.7 cM was constructed from a subset of the KBRIL (n = 142) using 130 single nucleotide polymorphisms. Two quantitative trait loci (QTL) for blast resistance, qBL1.1 harboring Pish/Pi35 and qBL2.1 harboring Pib, were mapped onto chromosomes 1 and 2, respectively. qBL1.1 was detected in both of the experimental sites, Namwon and Jeonju, while qBL2.1 was only detected in Namwon. qBL1.1 and qBL2.1 did not affect agronomic traits, including heading date, culm length, panicle length, and tiller number. From the 394 KBRILs, lines that were phenotypically similar to Koshihikari were selected according to heading date and culm length and were further divided into the following two groups based on blast resistance: Koshishikari-type blast resistant lines (KR, n = 15) and Koshishikari-type blast susceptible lines (KS, n = 15). Although no significant differences were observed in the major agronomic traits between the two groups, the KR group produced a greater mean head rice ratio than the KS group. The present study provides useful materials for developing blast-resistant cultivars that inherit both Koshihikari’s high eating quality and Baegilmi’s blast resistance.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"21 5 1","pages":"172-181"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76059839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Temperature and Daylength on Flowering and Growth Characteristics","authors":"K. Shim, B. Goo, Myoung Na Shin, W. Jeon","doi":"10.7740/KJCS.2020.65.3.241","DOIUrl":"https://doi.org/10.7740/KJCS.2020.65.3.241","url":null,"abstract":"Sesame is typically a temperatureand day length-sensitive plant, as its flowering is promoted under high temperature and shorter day length conditions. The experiment carried out in the present study revealed that day length influenced sesame flowering to a much greater extent than temperature. The degree of day length influence differed depending on the sesame variety. Days from sowing to flowering was negatively correlated with yield-related characteristics such as stem length, capsule number per plant, 1,000 seed weight, and seed weight per 10a. In cases where the period from sowing to flowering was longer and flowering date was later, the reproductive growth period was shorter during maturity. Analysis of the effects of temperature and day length on seed yield production revealed that temperature was a much more influential factor than day length. However, day length was more influential than temperature under low temperatures, such as 22°C. All varieties apart from Ansan were shown to be primarily influenced by temperature. Sesame varieties were grouped in view of optimal seed sowing time through principal components analysis. The Poongsung and Sungboon varieties favored early sowing under low temperatures and short day lengths. The DT45, Poongsung, 90 days, Ansan, and Sungboon varieties favored late sowing under high temperatures and long day lengths. The Yangbaek and Arum varieties could be sown both early and late.","PeriodicalId":22717,"journal":{"name":"The Korean Journal of Crop Science","volume":"22 1","pages":"241-247"},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80117797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}