The Journal of Biochemistry最新文献_第5页

A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I HLA I类o糖基化改变逃避CTL免疫的机制

The Journal of Biochemistry Pub Date : 2016-12-22 DOI: 10.1093/jb/mvw096

M. Sutoh Yoneyama, Y. Tobisawa, S. Hatakeyama, Misaki Sato, Kiyoshi Tone, Yota Tatara, I. Kakizaki, T. Funyu, M. Fukuda, S. Hoshi, C. Ohyama, S. Tsuboi

{"title":"A mechanism for evasion of CTL immunity by altered O-glycosylation of HLA class I","authors":"M. Sutoh Yoneyama, Y. Tobisawa, S. Hatakeyama, Misaki Sato, Kiyoshi Tone, Yota Tatara, I. Kakizaki, T. Funyu, M. Fukuda, S. Hoshi, C. Ohyama, S. Tsuboi","doi":"10.1093/jb/mvw096","DOIUrl":"https://doi.org/10.1093/jb/mvw096","url":null,"abstract":"Anti-tumour immunity by cytotoxic T-lymphocytes (CTLs) is essential to suppress tumour progression. Cancer cells that evade CTL immunity proliferate in the host, promoting metastasis, but mechanisms underlying this capacity remain unknown. Here we report that bladder cancer cells metastasized to lymph nodes evade CTL immunity by a new mechanism via altered glycosylation. CTLs normally recognize and kill cancer cells presenting antigenic peptides on human leukocyte antigen (HLA) class I. We show bladder cancer cells expressing the O-glycan processing enzyme, core2 β-1,6-N-acetylglucosaminyltransferase (C2GnT) exhibit HLA class I O-glycan modified with poly-N-acetyllactosamine and are highly susceptible to CTL. In those cells, poly-N-acetyllactosamine on HLA class I O-glycan binds galectin-3 to form a cell-surface molecular lattice, enabling efficient cell-surface retention of HLA class I. In contrast, bladder cancer cells in which C2GnT is downregulated show decreased levels of poly-N-acetyllactosamine on HLA class I O-glycans, attenuating lattice formation and reducing the cell-surface half-life of HLA class I. These tumour cells present antigenic peptides less efficiently, thereby evading CTL lysis and facilitating metastasis.","PeriodicalId":22605,"journal":{"name":"The Journal of Biochemistry","volume":"10 1","pages":"479–492"},"PeriodicalIF":0.0,"publicationDate":"2016-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80806989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Molecular cloning of rat acss3 and characterization of mammalian propionyl-CoA synthetase in the liver mitochondrial matrix 大鼠acss3的分子克隆及哺乳动物肝脏线粒体基质丙酰辅酶a合成酶的鉴定

The Journal of Biochemistry Pub Date : 2016-12-21 DOI: 10.1093/jb/mvw067

Y. Yoshimura, Aya Araki, Hitomi Maruta, Yoshitaka Takahashi, H. Yamashita

{"title":"Molecular cloning of rat acss3 and characterization of mammalian propionyl-CoA synthetase in the liver mitochondrial matrix","authors":"Y. Yoshimura, Aya Araki, Hitomi Maruta, Yoshitaka Takahashi, H. Yamashita","doi":"10.1093/jb/mvw067","DOIUrl":"https://doi.org/10.1093/jb/mvw067","url":null,"abstract":"Among the three acyl-CoA synthetase short-chain family members (ACSS), ACSS3 is poorly characterized. To characterize ACSS3, we performed molecular cloning and protein expression of rat acss3 and determined its intracellular localization, tissue distribution, and substrate specificity. Transient expression of rat ACSS3 in HeLa cells resulted in a 10-fold increase of acetyl-CoA synthetase activity compared with that in control cells. The acss3 transcripts are expressed in a wide range of tissues, with the highest levels observed in liver tissue followed by kidney tissue. Subcellular fractionation using liver tissue showed that ACSS3 is localized into the mitochondrial matrix. Among the short-chain fatty acids examined, recombinant ACSS3, purified from Escherichia coli cells transformed with the plasmid containing rat acss3, preferentially utilized propionate with a KM value of 0.19 mM. Knockdown of acss3 in HepG2 cells resulted in a significant decrease of ACSS3 expression level and propionyl-CoA synthetase activity in cell lysates. Levels of ACSS3 in the liver and the activity of propionyl-CoA synthetase in the mitochondria were significantly increased by fasting. These results suggested that ACSS3 is a liver mitochondrial matrix enzyme with high affinity to propionic acid, and its expression level is upregulated under ketogenic conditions.","PeriodicalId":22605,"journal":{"name":"The Journal of Biochemistry","volume":"11 1","pages":"279–289"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91150661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Characterization of C-S lyase from Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 and its potential role in food flavour applications 德氏乳杆菌C-S裂解酶的研究保加利亚菌ATCC BAA-365及其在食品香精应用中的潜在作用

The Journal of Biochemistry Pub Date : 2016-12-21 DOI: 10.1093/jb/mvw079

A. Allegrini, A. Astegno, V. La Verde, P. Dominici

{"title":"Characterization of C-S lyase from Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 and its potential role in food flavour applications","authors":"A. Allegrini, A. Astegno, V. La Verde, P. Dominici","doi":"10.1093/jb/mvw079","DOIUrl":"https://doi.org/10.1093/jb/mvw079","url":null,"abstract":"Volatile thiols have substantial impact on the aroma of many beverages and foods. Thus, the control of their formation, which has been linked to C-S lyase enzymatic activities, is of great significance in industrial applications involving food flavours. Herein, we have carried out a spectroscopic and functional characterization of a putative pyridoxal 5'-phosphate (PLP)-dependent C-S lyase from the lactic acid bacterium Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 (LDB C-S lyase). Recombinant LDB C-S lyase exists as a tetramer in solution and shows spectral properties of enzymes containing PLP as cofactor. The enzyme has a broad substrate specificity toward sulphur-containing amino acids with aminoethyl-L-cysteine and L-cystine being the most effective substrates over L-cysteine and L-cystathionine. Notably, the protein also reveals cysteine-S-conjugate β-lyase activity in vitro, and is able to cleave a cysteinylated substrate precursor into the corresponding flavour-contributing thiol, with a catalytic efficiency higher than L-cystathionine. Contrary to similar enzymes of other lactic acid bacteria however, LDB C-S lyase is not capable of α,γ-elimination activity towards L-methionine to produce methanethiol, which is a significant compound in flavour development. Based on our results, future developments can be expected regarding the flavour-forming potential of Lactobacillus C-S lyase and its use in enhancing food flavours.","PeriodicalId":22605,"journal":{"name":"The Journal of Biochemistry","volume":"20 1","pages":"349–360"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78491642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Evaluation of compound selectivity of aldo-keto reductases using differential scanning fluorimetry 差示扫描荧光法评价醛酮还原酶的化合物选择性

The Journal of Biochemistry Pub Date : 2016-12-21 DOI: 10.1093/jb/mvw063

Aurangazeb Kabir, S. Endo, N. Toyooka, Mayuko Fukuoka, K. Kuwata, Y. Kamatari

引用次数: 4

Conformational changes in inhibitory PAS domain protein associated with binding of HIF-1&agr; and Bcl-xL in living cells 抑制PAS结构域蛋白与HIF-1&agr结合的构象变化活细胞中的Bcl-xL

The Journal of Biochemistry Pub Date : 2016-12-21 DOI: 10.1093/jb/mvw068

S. Kasai, S. Kajimoto, Yuma Ito, T. Saito, K. Yasumoto, M. Tokunaga, K. Sakata-Sogawa, H. Fukumura, K. Sogawa

引用次数: 5

Gene cloning, recombinant expression, purification and characterization of L-methionine decarboxylase from Streptomyces sp. 590 链霉菌(Streptomyces sp. 590) l -蛋氨酸脱羧酶基因的克隆、重组表达、纯化及特性分析

The Journal of Biochemistry Pub Date : 2016-12-21 DOI: 10.1093/jb/mvw083

M. Hayashi, A. Okada, K. Yamamoto, Tomomi Okugochi, Chika Kusaka, Daizou Kudou, M. Nemoto, J. Inagaki, Yuu Hirose, T. Okajima, T. Tamura, K. Soda, K. Inagaki

{"title":"Gene cloning, recombinant expression, purification and characterization of L-methionine decarboxylase from Streptomyces sp. 590","authors":"M. Hayashi, A. Okada, K. Yamamoto, Tomomi Okugochi, Chika Kusaka, Daizou Kudou, M. Nemoto, J. Inagaki, Yuu Hirose, T. Okajima, T. Tamura, K. Soda, K. Inagaki","doi":"10.1093/jb/mvw083","DOIUrl":"https://doi.org/10.1093/jb/mvw083","url":null,"abstract":"l-Methionine decarboxylase (MetDC) from Streptomyces sp. 590 depends on pyridoxal 5'-phosphate and catalyzes the non-oxidative decarboxylation of l-methionine to produce 3-methylthiopropylamine and carbon dioxide. MetDC gene (mdc) was determined to consist of 1,674 bp encoding 557 amino acids, and the amino acid sequence is similar to that of l-histidine decarboxylases and l-valine decarboxylases from Streptomyces sp. strains. The mdc gene was cloned and recombinant MetDC was heterologously expressed by Escherichia coli. The purification of recombinant MetDC was carried out by DEAE-Toyopearl and Ni-NTA agarose column chromatography. The recombinant enzyme was homodimeric with a molecular mass of 61,000 Da and showed optimal activity between 45 to 55 °C and at pH 6.6, and the stability below 30 °C and between pH 4.6 to 7.0. l-Methionine and l-norleucine were good substrates for MetDC. The Michaelis constants for l-methionine and l-norleucine were 30 and 73 mM, respectively. The recombinant MetDC (0.50 U/ml) severely inhibited growth of human tumour cells A431 (epidermoid ovarian carcinoma cell line) and MDA-MB-231 (breast cancer cell line), however showed relatively low cytotoxicity for human normal cell NHDF-Neo (dermal fibroblast cell line from neonatal foreskin). This study revealed the properties of the gene and the protein sequence of MetDC for the first time.","PeriodicalId":22605,"journal":{"name":"The Journal of Biochemistry","volume":"1 1","pages":"389–398"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82275627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Distribution of glycosylinositol phosphoceramide-specific phospholipase D activity in plants 植物中糖基肌醇磷酸神经酰胺特异性磷脂酶D活性的分布

The Journal of Biochemistry Pub Date : 2016-11-08 DOI: 10.1093/jb/mvw060

T. Kida, Aoi Itoh, A. Kimura, H. Matsuoka, H. Imai, K. Kogure, A. Tokumura, Tamotsu Tanaka

引用次数: 7

Structure models of G72, the product of a susceptibility gene to schizophrenia 精神分裂症易感基因G72的结构模型

The Journal of Biochemistry Pub Date : 2016-11-03 DOI: 10.1093/jb/mvw064

Y. Kato, K. Fukui

{"title":"Structure models of G72, the product of a susceptibility gene to schizophrenia","authors":"Y. Kato, K. Fukui","doi":"10.1093/jb/mvw064","DOIUrl":"https://doi.org/10.1093/jb/mvw064","url":null,"abstract":"The G72 gene is one of the most susceptible genes to schizophrenia and is contained exclusively in the genomes of primates. The product of the G72 gene modulates the activity of D-amino acid oxidase (DAO) and is a small protein prone to aggregate, which hampers its structural studies. In addition, lack of a known structure of a homologue makes it difficult to use the homology modelling method for the prediction of the structure. Thus, we first developed a hybrid ab initio approach for small proteins prior to the prediction of the structure of G72. The approach uses three known ab initio algorithms. To evaluate the hybrid approach, we tested our prediction of the structure of the amino acid sequences whose structures were already solved and compared the predicted structures with the experimentally solved structures. Based on these comparisons, the average accuracy of our approach was calculated to be ∼5 Å. We then applied the approach to the sequence of G72 and successfully predicted the structures of the N- and C-terminal domains (ND and CD, respectively) of G72. The predicted structures of ND and CD were similar to membrane-bound proteins and adaptor proteins, respectively.","PeriodicalId":22605,"journal":{"name":"The Journal of Biochemistry","volume":"10 1","pages":"223–230"},"PeriodicalIF":0.0,"publicationDate":"2016-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84041669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

An MCM4 mutation detected in cancer cells affects MCM4/6/7 complex formation 在癌细胞中检测到的MCM4突变影响MCM4/6/7复合物的形成

The Journal of Biochemistry Pub Date : 2016-10-29 DOI: 10.1093/jb/mvw065

R. Tatsumi, Y. Ishimi

引用次数: 10

Mutational analysis and overproduction effects of MotX, an essential component for motor function of Na+-driven polar flagella of Vibrio 弧菌Na+驱动极性鞭毛运动功能必需成分MotX的突变分析及过量产生效应

The Journal of Biochemistry Pub Date : 2016-10-25 DOI: 10.1093/jb/mvw061

N. Takekawa, S. Kojima, M. Homma

引用次数: 2