Eye Banking and Corneal Transplantation最新文献

Screening Potential Cornea Donors for Signs of Sepsis 筛查角膜捐献者是否有败血症迹象

Eye Banking and Corneal Transplantation Pub Date : 2024-07-12 DOI: 10.1097/ebct.0000000000000026

Paul Chong, Kate Marsh, D. Vavra, W. C. Fowler, Robert W. Enzenauer

{"title":"Screening Potential Cornea Donors for Signs of Sepsis","authors":"Paul Chong, Kate Marsh, D. Vavra, W. C. Fowler, Robert W. Enzenauer","doi":"10.1097/ebct.0000000000000026","DOIUrl":"https://doi.org/10.1097/ebct.0000000000000026","url":null,"abstract":"\u0000 \u0000 This study sought to expand upon previous works to explore correlations between cornea donor characteristics and incidence of sepsis/donor ineligibility.\u0000 \u0000 \u0000 \u0000 The medical records of 837 potential cornea donors with signs of possible sepsis from 2 Eye Bank Association of America–accredited eye banks between 2009 and 2022 were reviewed by an infectious disease clinician consultant for US Food and Drug Administration-designated signs of sepsis. Data and machine learning analysis were performed to explore relationships between different donor features and donor eligibility.\u0000 \u0000 \u0000 \u0000 A review of the potential cornea donor records revealed that the presence of a greater number of signs of sepsis was not associated with a higher probability of donor ineligibility (P = 0.45). The only single sign of sepsis that was associated with a higher likelihood of donor ineligibility determination was positive blood cultures (P < 0.001). Finally, if the patient's length of hospitalization was less than 24 hours, these potential donors were more likely to be deemed ineligible for cornea donation (P < 0.001). Machine learning analysis revealed length of hospitalization to be the most importantly weighted factor in determining donor eligibility.\u0000 \u0000 \u0000 \u0000 Based on our analysis of hundreds of medical records over more than a decade, the data suggest that potential cornea donors who 1) have a positive blood culture or 2) have a length of stay of less than 24 hours may be reasonably deemed likely not eligible for donation and not all forwarded to infectious disease consultants or their respective medical director. The results of this study call for further exploration with the collection of larger sample sizes and more representative populations of cornea donors to pursue potential protocols with respect to determining cornea donor eligibility.\u0000","PeriodicalId":225499,"journal":{"name":"Eye Banking and Corneal Transplantation","volume":"21 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141653979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cornea and Eye Banking Forum 2023 Scientific Abstracts: Proceedings of the November 2023 Meeting, Cosponsored by the Eye Bank Association of America and the Cornea Society 角膜和眼库论坛 2023 年科学摘要：美国眼库协会和角膜协会共同主办的 2023 年 11 月会议论文集

Eye Banking and Corneal Transplantation Pub Date : 2024-07-12 DOI: 10.1097/ebct.0000000000000028

引用次数: 0

Donor Cornea Storage Temperature: Investigation of the Time Period Immediately Before Cornea Transplantation Surgery 捐献角膜的储存温度：角膜移植手术前一段时间的调查

Eye Banking and Corneal Transplantation Pub Date : 2024-03-01 DOI: 10.1097/ebct.0000000000000023

Karl Maeser Andersen, Joseph Lin Da, W. M. Petroll, William Hatcher Waldrop

{"title":"Donor Cornea Storage Temperature: Investigation of the Time Period Immediately Before Cornea Transplantation Surgery","authors":"Karl Maeser Andersen, Joseph Lin Da, W. M. Petroll, William Hatcher Waldrop","doi":"10.1097/ebct.0000000000000023","DOIUrl":"https://doi.org/10.1097/ebct.0000000000000023","url":null,"abstract":"\u0000 \u0000 Donor cornea storage temperature seems to affect tissue viability; however, the time period between removal from storage and transplant surgery has not been explored. We investigated the possible impact time at room temperature in the operating room may have on endothelial cell density (ECD) and corneal thickness (CT).\u0000 \u0000 \u0000 \u0000 In our simulation, transplant-ineligible corneas underwent 1 of 4 room temperature protocols. ECD (by specular microscopy) and CT (by anterior segment optical coherence tomography [OCT]), measured before and after protocols, were used to explore possible effects of time at room temperature. Control group tissue resided at room temperature only long enough for warming and subsequent ECD and CT measurements.\u0000 \u0000 \u0000 \u0000 Corneas underwent one of the following protocols: 1) 17 (±1) minutes at room temperature (for baseline OCT measurements; n = 13) followed by 23 minutes at 35°C (to facilitate specular imaging, conducted for all 4 protocols), 2) 60 (±1) minutes (1 hour; n = 8) at room temperature, 3) 187 (±1) minutes (approximately 3 hours; n = 7) at room temperature, and 4) 416 (±1) minutes (approximately 7 hours; n = 7). Statistical analysis showed no significant difference in ECD change (P = 0.353) or CT change (P = 0.415) between protocol groups. Controlling for donor age and death-to-preservation time rendered no statistical significance.\u0000 \u0000 \u0000 \u0000 Time at room temperature did not seem to significantly influence ECD or CT measured soon after warming. Studies exploring ECD postoperatively and long-term graft survival are needed to assess the possible clinical impact of preoperative time at room temperature.\u0000","PeriodicalId":225499,"journal":{"name":"Eye Banking and Corneal Transplantation","volume":"43 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140276442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qualitative Blade Topology in Donor Cornea Punches and Corresponding Endothelial Cell Loss in PK and DMEK Grafts 供体角膜切口的定性刀片拓扑及 PK 和 DMEK 移植中相应的内皮细胞损失

Eye Banking and Corneal Transplantation Pub Date : 2024-03-01 DOI: 10.1097/ebct.0000000000000022

M. Straiko, Claudia S. López, R. Hubbs, Phillip K Dye, Khoa D. Tran

{"title":"Qualitative Blade Topology in Donor Cornea Punches and Corresponding Endothelial Cell Loss in PK and DMEK Grafts","authors":"M. Straiko, Claudia S. López, R. Hubbs, Phillip K Dye, Khoa D. Tran","doi":"10.1097/ebct.0000000000000022","DOIUrl":"https://doi.org/10.1097/ebct.0000000000000022","url":null,"abstract":"\u0000 \u0000 Two commonly used donor cornea punches were assessed using quantification of peripheral endothelial cell loss (ECL) and scanning electron microscopy (SEM) of blade topology and associated features on donor grafts.\u0000 \u0000 \u0000 \u0000 Penetrating keratoplasty (PK) (n = 8 pairs) and Descemet membrane endothelial keratoplasty (DMEK) (n = 8 pairs) grafts were prepared from mate donor corneas using Moria Busin or Corza Barron donor cornea punches (all punches were 7.5 mm) and analyzed for global and peripheral ECL. Punches were examined by SEM and subsequently used to prepare PK grafts from mate donor corneas (n = 3 pairs) that were also assessed by SEM.\u0000 \u0000 \u0000 \u0000 For PK grafts, peripheral ECL caused by Busin and Barron punches was 4.2% ± 0.6% and 4.0% ± 0.9% (P = 0.62), respectively. For DMEK grafts, peripheral ECL caused by Busin and Barron punches was 3.6% ± 1.9% and 3.0% ± 1.3% (P = 0.48), respectively. SEM analysis of the Busin punch identified a rolled blade with a seam; the Barron punch is created from full rod stock and is seamless. Busin punch PK grafts have a ridge of stromal tissue that was not observed in the Barron PK grafts. Both punches exhibit anomalies along the blade edge, and the stroma of both grafts exhibited “drag lines.”\u0000 \u0000 \u0000 \u0000 Both punches caused similar and minimal ECL. In PK grafts, the seam of the Busin punch may be the source of the observed uneven stromal surface. Striations and debris on the cutting surfaces of both punches may produce drag lines in cut stroma. These topological features may contribute to uneven donor–recipient tissue apposition, leading to astigmatism after PK.\u0000","PeriodicalId":225499,"journal":{"name":"Eye Banking and Corneal Transplantation","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140276524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstracts from the 2023 Summer Scientific Symposium of the Eye Bank Association of America 美国眼库协会 2023 年夏季科学研讨会摘要

Eye Banking and Corneal Transplantation Pub Date : 2023-12-01 DOI: 10.1097/ebct.0000000000000016

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Comparison of Corneal Endothelial Cell Density and Morphology With Optisol-GS and Life4C Storage Media in the Eye Bank: A 5-Year Retrospective Analysis 眼库中使用 Optisol-GS 和 Life4C 储存介质的角膜内皮细胞密度和形态比较：5年回顾性分析

Eye Banking and Corneal Transplantation Pub Date : 2023-12-01 DOI: 10.1097/ebct.0000000000000019

Rubens Petithomme, Ranit Karmakar, John Lohmeier, Staci Terrin, Ellen H Koo, A. Eghrari

{"title":"Comparison of Corneal Endothelial Cell Density and Morphology With Optisol-GS and Life4C Storage Media in the Eye Bank: A 5-Year Retrospective Analysis","authors":"Rubens Petithomme, Ranit Karmakar, John Lohmeier, Staci Terrin, Ellen H Koo, A. Eghrari","doi":"10.1097/ebct.0000000000000019","DOIUrl":"https://doi.org/10.1097/ebct.0000000000000019","url":null,"abstract":"\u0000 \u0000 Optisol-GS and Life4C are corneal storage media used by eye banks worldwide. We sought to determine whether either solution was associated with superior corneal endothelial cell density (ECD) or morphology in a large cohort of donor corneas.\u0000 \u0000 \u0000 \u0000 From January 2016 to December 2020, 10,316 corneas from 5624 unique donors were acquired and analyzed at Rocky Mountain Lions Eye Bank. In April 2019, Life4C replaced Optisol-GS as the sole storage medium. We compared ECD and morphology before and after April 2019 and excluded corneas processed within the transition period. Univariable and multivariable regression analyses accounted for age, sex, tobacco use, heavy alcohol use, and diabetes. Only right corneas were analyzed to account for the correlation between eyes.\u0000 \u0000 \u0000 \u0000 Of 5042 right corneas analyzed, 3486 were stored in Optisol-GS and 1556 in Life4C. There was no significant difference in ECD across groups (2794 vs. 2793 cells/mm2 in Optisol-GS and Life4C, P = 0.88). In multivariate analyses, there was no significant difference in corneal ECD (0.6 cells/mm2 higher with Life4C, P = 0.96) or hexagonality (0.22% higher with Life4C, P = 0.31). However, the coefficient of variation was significantly lower in the Life4C group (−0.0039, P = 0.03). After adjustment for above factors, corneas in Life4C demonstrated a 3.1% decreased likelihood of exhibiting coefficient of variation (CV) values greater than 0.40 (P = 0.009).\u0000 \u0000 \u0000 \u0000 This study demonstrates comparable and favorable outcomes using both storage media and confirms their overall efficacy. The decreased CV in Life4C is not of clinically significant magnitude but merits further research in clinical and long-term settings.\u0000","PeriodicalId":225499,"journal":{"name":"Eye Banking and Corneal Transplantation","volume":"75 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139024805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of SkQ1 on the Oxidative Stress and Inflammatory Responses in Corneal Endothelial Cells During Ex Vivo Expansion SkQ1 对角膜内皮细胞体外扩张过程中氧化应激和炎症反应的影响

Eye Banking and Corneal Transplantation Pub Date : 2023-12-01 DOI: 10.1097/ebct.0000000000000017

Charlene H. Choo, D. Chung, A. Aldave

{"title":"The Effect of SkQ1 on the Oxidative Stress and Inflammatory Responses in Corneal Endothelial Cells During Ex Vivo Expansion","authors":"Charlene H. Choo, D. Chung, A. Aldave","doi":"10.1097/ebct.0000000000000017","DOIUrl":"https://doi.org/10.1097/ebct.0000000000000017","url":null,"abstract":"\u0000 \u0000 The purpose of this study was to characterize the oxidative stress and inflammatory responses in ex vivo corneal endothelial cells (evCEnC) during expansion and assess the impact of SkQ1, an antioxidant and anti-inflammatory compound, on measures of oxidative stress and inflammation.\u0000 \u0000 \u0000 \u0000 A CEnC line (HCEnC-21T) was cultured in media supplemented with varying SkQ1 concentrations to determine the optimal SkQ1 dose range of toxicity and protective effect on CEnC exposed to acute oxidative stress. The impact of SkQ1 treatment on intracellular free radical (IFR) levels, NRF2-mediated oxidative stress response, and NFkB-mediated inflammatory response was determined at each passage of evCEnC isolated from donor corneas and cultured in SkQ1-supplemented and untreated media.\u0000 \u0000 \u0000 \u0000 HCEnC-21T cultured in media supplemented with ≤250 nM SkQ1 retained over 95% cell viability compared with untreated cells. SkQ1 provided oxidative stress protection to HCEnC-21T in a dose-dependent manner up to 500 nM. In evCEnC, 50 nM and 250 nM SkQ1 supplementation significantly reduced IFR levels across passages 0 to 3 (P-values of 0.015 and 0.023, respectively) and 50 nM SkQ1 supplementation led to decreased levels of active NRF2 in evCEnC at passage 2. However, media supplementation with SkQ1 (50 nM and 250 nM) did not alter NFkB activation at any passage.\u0000 \u0000 \u0000 \u0000 SkQ1 media supplementation provides oxidative stress protection to HCEnC-21T in a dose-dependent manner and decreases IFR levels and NRF2 activation in evCEnC during expansion at concentrations that do not negatively affect CEnC viability. These findings indicate that SkQ1 supplementation may increase the expansion potential of evCEnC.\u0000","PeriodicalId":225499,"journal":{"name":"Eye Banking and Corneal Transplantation","volume":"121 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Head Trauma Effect on Corneal Endothelial Cell Density in Eye Bank Donors 50 Years or Older 头部创伤对 50 岁或以上眼库捐赠者角膜内皮细胞密度的影响

Eye Banking and Corneal Transplantation Pub Date : 2023-12-01 DOI: 10.1097/ebct.0000000000000020

Jessinta Oseni, Jenna Tauber, Gabriel Rand, Jee Young Moon, Patrick K. Gore, Brandon Edwards, Tina Livesay, Anthony Vizzerra, Roy S. Chuck

{"title":"Head Trauma Effect on Corneal Endothelial Cell Density in Eye Bank Donors 50 Years or Older","authors":"Jessinta Oseni, Jenna Tauber, Gabriel Rand, Jee Young Moon, Patrick K. Gore, Brandon Edwards, Tina Livesay, Anthony Vizzerra, Roy S. Chuck","doi":"10.1097/ebct.0000000000000020","DOIUrl":"https://doi.org/10.1097/ebct.0000000000000020","url":null,"abstract":"\u0000 \u0000 The purpose of this study was to determine the effect of head trauma on corneal endothelial cell density (ECD) in eye bank donors 50 years or older.\u0000 \u0000 \u0000 \u0000 This is a retrospective study of 128 corneas from 67 donors 50 years or older with cause of death secondary to head trauma, gunshot wound to the head (n = 26), motor vehicle accident with head trauma (n = 33), a fall with sustained head trauma (n = 47), and non-head trauma–related deaths (n = 22). Corneal data were recovered from Saving Sight Eye Bank (Kansas City, MO) database between January 2018 and August 2021. Donor characteristics, ECD, and focal endothelial cell loss on specular microscopy were examined between groups. A linear mixed effects model and a mixed effects logistic regression were used to compare ECD and examine the association between head trauma and focal endothelial cell loss.\u0000 \u0000 \u0000 \u0000 The average ECD in the head trauma group was 2471 ± 342 cells/mm2 and 2588 ± 282 cells/mm2 in the nonhead trauma group (P value = 0.24). After adjusting for age, death-to-preservation time, and pseudophakia, the ECD difference between the head trauma and nonhead trauma groups was −105 ± 112 cells/mm2 (P value = 0.35). The odds of having focal endothelial cell loss were not statistically significant (P value = 0.42) between the head trauma (odds = 0.93) and nonhead trauma (odds = 0.69) groups.\u0000 \u0000 \u0000 \u0000 There were no differences in ECD and focal endothelial cell loss between the head trauma and nonhead trauma groups in eye bank donors 50 years or older.\u0000","PeriodicalId":225499,"journal":{"name":"Eye Banking and Corneal Transplantation","volume":"35 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139016270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Evolving Therapeutics of Endothelial Disease 内皮疾病的新疗法

Eye Banking and Corneal Transplantation Pub Date : 2023-08-22 DOI: 10.1097/ebct.0000000000000015

Marcela Huertas-Bello, Alfonso L. Sabater, E. H. Koo

引用次数: 0

Comparison of Five-Year Graft Survival After Keratoplasty in the Veteran Population 退伍军人角膜移植术后5年移植物存活率的比较

Eye Banking and Corneal Transplantation Pub Date : 2023-08-21 DOI: 10.1097/ebct.0000000000000010

Sydney P. Wendt, A. Gordon, Anshul Bhatnagar, S. Orengo‐Nania, K. Biggerstaff, Sumitra S. Khandelwal

{"title":"Comparison of Five-Year Graft Survival After Keratoplasty in the Veteran Population","authors":"Sydney P. Wendt, A. Gordon, Anshul Bhatnagar, S. Orengo‐Nania, K. Biggerstaff, Sumitra S. Khandelwal","doi":"10.1097/ebct.0000000000000010","DOIUrl":"https://doi.org/10.1097/ebct.0000000000000010","url":null,"abstract":"\u0000 \u0000 We investigated the corneal graft survival rate after penetrating keratoplasty (PK) and Descemet stripping endothelial keratoplasty (DSEK) in a veteran population.\u0000 \u0000 \u0000 \u0000 A retrospective review was conducted on patients undergoing a primary PK or DSEK at a large Veterans Affairs Medical Center between January 2005 and August 2019. Exclusion criteria included patients with a postoperative follow-up less than 5 years and patients undergoing additional ocular procedures during surgery. Patients were classified into 3 groups depending on their glaucoma status before corneal transplant: no pre-existing glaucoma, medically managed glaucoma, or surgically managed glaucoma. Graft survival was calculated using the Kaplan–Meier survival analysis.\u0000 \u0000 \u0000 \u0000 A total of 30 eyes underwent PK, and 28 eyes underwent DSEK. There was no significant difference in graft survival rates between PK and DSEK at 1 year (83% vs. 79%, P = 0.555), 3 years (53% vs. 64%, P = 0.536), and 5 years (40% vs. 54%, P = 0.399). When combining PK and DSEK groups, Kaplan–Meier analysis showed a highly significant difference in 5-year graft survival between patients without pre-existing glaucoma (58%) and patients with either medically (50%) or surgically managed glaucoma (0%).\u0000 \u0000 \u0000 \u0000 We find approximate parity in 1-year, 3-year, and 5-year survival rates after PK and DSEK within the veteran population. However, survival rates for both procedures are lower than those previously reported within other populations. Additional studies to understand these differences in larger cohorts are warranted. Ophthalmologists must understand the increased risk of graft failure in the veteran population to provide appropriate counseling.\u0000","PeriodicalId":225499,"journal":{"name":"Eye Banking and Corneal Transplantation","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123922766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0