Journal of Diagnostic Pathology最新文献

{"title":"Expression of epithelial and mesenchymal markers in invasive (ductal) breast carcinoma and nodal metastasis","authors":"L. Y. V. Pathirana, E. Siriweera, H. Amaratunga, S. Adikari, N. Gunasinghe","doi":"10.4038/JDP.V11I2.7710","DOIUrl":"https://doi.org/10.4038/JDP.V11I2.7710","url":null,"abstract":"Introduction The phenomenon, Epithelial-mesenchymal transition (EMT) has been shown to enable epithelial cells to acquire a mesenchymal phenotype facilitating cancer cell mobilization. Evidence in favour has been derived predominantly from cancer cell lines and/or animal model studies. The objectives of this study were to observe E-cadherin and vimentin expression patterns in invasive (ductal) breast carcinoma-no special type (BCa) and corresponding metastatic lymph nodes (mLNs) and to compare the expression of these markers in BCa with and without metastasis using clinical samples. Methodology Tissues of 24 metastatic BCa (mBCa) with mLNs and 17 non metastatic BCa (nmBCa) obtained from the Department of Pathology, Faculty of Medicine, Peradeniya were included. Immunohistochemical expression of E-cadherin (epithelial cell adhesion membrane marker) and vimentin (mesenchymal cytoplasmic marker) was observed on all BCas and mLNs. Ten random high-power fields (10x40) of 100 cells each were assessed to determine the percentage of positive cells . The mean expression of markers was compared between mBCa and nmBCa by independent sample T-test and mBCa and mLN by paired sample T-test. Results The mean E-cadherin expression was 70.54% (27.67%-97.67%) in mBCa, 86.40% (63.33%-96.67%) in nmBCa and 89.95% (46.33%-100%) in mLNs. The mean vimentin expression was 6.54% (2.37%-13.87%) in mBCa, 5.39% (2.12%- 9.77%) in nmBCa and 1.28% (0.13%-8.25%) in mLNs. The E-cadherin expression was significantly lower in mBCa compared to nmBCa (p=0.002) and in mBCa compared to mLNs (p=0.000). Vimentin expression was not significant between mBCA and nmBCa (p=0.176). Higher vimentin expression in mBCa compared to mLNs was statistically significant (p=0.000). Discussion and conclusion The significantly lower expression of E-cadherin in mBCa compared to nmBCa suggests that loss of epithelial cell properties promotes metastasis. Significantly lower E-cadherin and significantly higher vimentin expressions in mBCa compared to mLNs is supportive of EMT in BCa. The reversed pattern is noted in nodal BCa metastasis suggesting the possibility of mesenchymal-epithelial transition of metastatic cancer cells in the lymph node.","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131138651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined hepatocellular- cholangiocarcinoma: a diagnostic challenge","authors":"E. Wickramaratne, A. Dharmapala, S. Wijetunge","doi":"10.4038/JDP.V11I2.7707","DOIUrl":"https://doi.org/10.4038/JDP.V11I2.7707","url":null,"abstract":"Introduction Combined hepatocellularcholangiocarcinoma (cHCC-CC) is a rare primary liver tumour with histopathological features of both hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC) (1). This neoplasm demonstrates unequivocal, intimately mixed elements of HCC and CC (2). In collision tumours HCC is juxtaposed with CC without intermingling of the two elements, hence excluded in the WHO classification of cHCC-CC (3).","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123947389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triple-negative breast cancers – update in classification and biomarker profile","authors":"Thazin Hlaing, Sio-In Wong, J. Li, J. Tsang, G. Tse","doi":"10.4038/JDP.V11I2.7704","DOIUrl":"https://doi.org/10.4038/JDP.V11I2.7704","url":null,"abstract":"Invasive breast carcinoma (IBC) is a heterogeneous disease, with more than 20 distinct histologic types recognized by the World Health Organization (WHO) classification (1). In addition to immunohistochemical (IHC) methods, molecular techniques have yielded information on the genetic characteristics of IBC by gene expression profiling (GEP), adding another perspective onto IBC classification. Five intrinsic subtypes of IBC: luminal A, luminal B, normal breast-like, HER2-enriched, and basallike have been identified, with demonstrable correlation with patient demographics, disease biologic behaviour and response to treatment (2, 3, 4).","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122008538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pathological profile of endometrial carcinoma in a cohort of Sri Lankan patients","authors":"N. Samarakoon, L. B. D. J. Siriwikum, S. Liyanage, B. Mahendra, R. Rathnayake, S. Hewavisenthi","doi":"10.4038/JDP.V11I2.7720","DOIUrl":"https://doi.org/10.4038/JDP.V11I2.7720","url":null,"abstract":"Introduction Endometrial cancer is the eighth most common cancer in Sri Lankan women. An understanding of the disease pattern of a local population would be useful in further management. The aim was to describe the pathological features and stage at presentation of endometrial carcinoma (EC) in a cohort of Sri Lankan patients. Methodology This is a retrospective study of 63 patients in the Western Province diagnosed with EC within the last five years. The macroscopic and microscopic features, grade and stage were obtained from the histopathology reports. The correlation between these parameters was assessed using the Pearson correlation coefficient (r). Results The median age at presentation was 62 years (range 35-78). Out of the 63 patients, 38(60%) were above 60 years, 52(83%) were polypoidal tumours and 11(17%) showed diffuse thickening of the endometrium. Histological types were endometrioid in 56/63(89%), serous in 5/63(8%) and mixed endometrioid mucinous adenocarcinomas in 2/63(3%). The EC comprised 36/63(57%) grade 1, 14/63(22%) grade 2 and 13/63(21%) grade 3 tumours. Lympho-vascular invasion (LVI), focal necrosis and squamoid differentiation was identified in 9/63 (14%), 24/63 (38%) and 15/63 (22%) respectively. The number of patients presenting in stages IA and IB were equal being 24/63(38%). Stage 2, 3A, 3B and 3C tumours included 7/63(11%), 5/63(8%), 2/63(3%) and 1/63(2%) respectively. No stage 4 tumours were recorded. There was a strong correlation between the stage and LVI(r=0.609), a moderate correlation between the grade and the LVI (r=0.412) and the grade and stage (r=0.410). There was no correlation between the age and stage(r=0.016). Conclusion Majority of endometrial carcinomas are of low grade and stage at diagnosis conferring a good prognosis and treatment outcome in our patients. Lympho-vascular invasion is significantly associated with a higher stage.","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123767484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge and attitudes of the cytology test for cervical cancer screening in a rural population in Sri Lanka - a population based survey","authors":"A. Priyani, P. Thuvarakan, U. Senarath","doi":"10.4038/JDP.V11I2.7713","DOIUrl":"https://doi.org/10.4038/JDP.V11I2.7713","url":null,"abstract":"Introduction Despite having a national cervical cancer(CC) screening programme, CC is still the second leading cancer among Sri Lankan females. The aim of this study was to assess knowledge and attitudes of the cytology test for CC screening in a rural population. Methodology HETC Rural Health Project, Faculty of Medicine, Colombo used a 2-stage stratified cluster sample of 1950 households from 13 divisional secretariats in Ampara, Monaragala, Hambanthota and Kalmunai to interview married females aged 35-65 years using an interviewer administered questionnaire. Results Total of 1499 females were interviewed. The mean age at marriage was 22 years. There were 1053(70%) housewives and 278(18%) were involved in agriculture, forestry or fishery industries. Of those who disclosed ethnicity (n=1397), 1115(80%), 191(14%) and 89(6%) were Sinhalese, Muslims and Tamils respectively. Of those who disclosed education level (n=1484), 1337(90%) had not passed ordinary level. Questions related to knowledge and attitudes of the cytology test were answered by 1233 participants. The awareness of the test in the age categories 35-40 years (n=311) and 41-45years (n=215) were 249 (80%) and 160(74%) respectively; the awareness decreased with the advancing age. Of 1233 participants 888(72%) have not done the test as, 448(50%) thought it was unnecessary as they did not have an illness, 306(34%) were unaware of the facility, 69(8%) were afraid of the procedure and 69(8%) had “practical difficulties”. Cultural/ religious reasons, attitudes of parents and healthcare workers were reasons in less than 2.5%. These proportions remained more or less the same for all education levels, ethnic groups and occupation. Conclusion The majority of the participants were aware of the screening test. However, negative attitudes resulted in failure to undergo cervical cancer screening test in a significant proportion, which can be corrected by a simple intervention like distributing a leaflet.","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134400574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using pathology reports as a data source for strengthening cancer surveillance","authors":"Niranthi Perera","doi":"10.4038/JDP.V11I1.7688","DOIUrl":"https://doi.org/10.4038/JDP.V11I1.7688","url":null,"abstract":"","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121819063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ovarian collision tumour in a postmenopausal woman mimicking malignancy","authors":"B. Thilakarathne, E. Siriweera","doi":"10.4038/JDP.V11I1.7695","DOIUrl":"https://doi.org/10.4038/JDP.V11I1.7695","url":null,"abstract":"Author for correspondence: B.M.I.K. Thilakarathne (MBBS, Dpath), Department of Pathology, Faculty of Medicine, University of Peradeniya, Sri Lanka. E mail: ireshakvithanage@yahoo.com Pathological features The specimen comprised a left ovarian tumour (25cm in diameter) with an attached fallopian tube and the uterus with attached right adnexa. The ovarian tumour had a smooth and gray‐white surface. Sectioning revealed a unilocular cystic component with a smooth inner surface (15cm in diameter) and a firm, homogenous, white and solid component (14x10x8 cm) (Figure1B). The uterus showed two intramural fibroids and an endometrial polyp. The right ovary and both tubes were unremarkable.","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122417801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental pathology in birth weight discordant monochorionic and dichorionic twins","authors":"A. Priyani, N. M. Gamage, T. Dias, M. V. C. D. Silva","doi":"10.4038/JDP.V11I1.7690","DOIUrl":"https://doi.org/10.4038/JDP.V11I1.7690","url":null,"abstract":"Identification of placental parenchymal lesions (PPL) associated with birth weight (BW) discordance will be helpful in understanding the pathogenesis of BW discordance in twins. The aim of this study was to evaluate such association in monochorionic (MC) and dichorionic (DC) twins. This was a single centre, prospective study of placentas of consecutive twin deliveries. Gross and microscopic examination of placentas were done according to a protocol. Association of PPL count with the degree of BW discordance and the significance of the presence of each PPL in the lighter twin (LT) in comparison to the heavier twin (HT) were analysed. Of the 92 placentas studied 57.6% were DC and 42.4% were MC. Significant BW discordance was present in 28% (n=39) of MC and 26% (n=53) of DC twins. There was a positive correlation with the PPL count of the LT and the mean BW discordance in both MC (p=0.032) and DC twins (p=0.043). Increased incidence of distal villous hypoplasia in the placental territory of the LT in comparison to the HT was statistically significant in BW-discordant DC twins (P=0.04) and in both BW-concordant and BW-discordant MC twins (p=0.02 and p=0.03 respectively). In conclusion, this single centre prospective study showed a strong association between the PPL count and the degree of BW discordance in both MC and DC twins. Increased incidence of distal villous hypoplasia in the placental territory of the LT in comparison to the HT was statistically significant.","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128593541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dr Meliyanthi Megasuriya Gunatillaka","authors":"D. Siriwardhana","doi":"10.4038/JDP.V11I1.7696","DOIUrl":"https://doi.org/10.4038/JDP.V11I1.7696","url":null,"abstract":"","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128153750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A histopathological analysis of granulomatous dermatoses – a single centre experience from Sri Lanka","authors":"M. Jayawardhana, R. T. A. W. Gunewardhana, N. Ratnatunga, M. Dissanayake","doi":"10.4038/JDP.V11I1.7691","DOIUrl":"https://doi.org/10.4038/JDP.V11I1.7691","url":null,"abstract":"Granulomatous inflammation is a common histological pattern encountered in skin biopsies which pose a diagnostic challenge to pathologists because of overlapping histological features produced by various aetiological agents. We conducted the following study to analyze the aetiological factors and morphological patterns of granulomatous dermatoses in a cohort of Sri Lankan patients from the Central Province. This is a retrospective analysis of skin biopsies dectected to have granulomatous inflammation over a 12 year period at the Department of Pathology, Faculty of Medicine, University of Peradeniya. All biopsies had been assessed using haematoxylin and eosin stain and special stains when necessary. Of the 1547 skin biopsies received, 128 (8.3%) were recognized to have granulomatous inflammation. An infectious aetiology was present in 86.7% (111/128).The most prevalent infectious cause was leprosy, accounting for 39.8% (51/128) followed by the category differential diagnoses in 22 (17.1%) of which had an infectious differential. Cutaneous tuberculosis was the cause in 17 (13.3%) cases. In 102 cases (79.7%) necrosis was absent in the granulomata and when present most were (58%) of suppurative type; typical caseous type necrosis was present in only a few cases. In conclusion, a large majority of granulomatous inflammation is due to infectious causes and leprosy is the leading cause in this study cohort.","PeriodicalId":225127,"journal":{"name":"Journal of Diagnostic Pathology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122925985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}