Molecular pathology and funct. genomics最新文献

LSC - 2020 - The role of the COPD susceptibility gene FAM13A in barrier function and pro-inflammatory responses of human airway epithelial cells 慢性阻塞性肺病易感基因FAM13A在人气道上皮细胞屏障功能和促炎反应中的作用

Molecular pathology and funct. genomics Pub Date : 2020-09-07 DOI: 10.1183/13993003.congress-2020.909

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LSC - 2020 - Longitudinal effect of smoking cessation on transcriptomics and epigenetics in the airways of COPD patients and asymptomatic controls LSC - 2020 -戒烟对COPD患者和无症状对照组气道转录组学和表观遗传学的纵向影响

Molecular pathology and funct. genomics Pub Date : 2020-09-07 DOI: 10.1183/13993003.congress-2020.2711

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LSC - 2020 - Mitochondrial dysfunction induce immunoproteasome and MHC class I responses in lung aging LSC - 2020 -线粒体功能障碍在肺衰老中诱导免疫蛋白酶体和MHC I类反应

Molecular pathology and funct. genomics Pub Date : 2020-09-07 DOI: 10.1183/13993003.congress-2020.328

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Versatile workflow for storage, characterization and cell-type resolved transcriptional and epigenetic profiles of human lung samples (LSC-2020) 用于人肺样本的存储、表征和细胞类型解析转录和表观遗传谱的通用工作流程(LSC-2020)

Molecular pathology and funct. genomics Pub Date : 2020-09-07 DOI: 10.1183/13993003.congress-2020.2713

M. Llamazares, Elisa Espinet, V. Mijošek, U. Schwartz, S. Waszak, P. Lustik, R. Tamas, M. Richter, A. Behrendt, S. Pohl, N. Benz, T. Muley, A. Warth, C. Heussel, Felix Hearth, T. Mertens, H. Karmouty-quintana, I. Koch, V. Beneš, J. Korbel, A. Trumpp, David Wyatt, H. Stahl, C. Plass, R. Jurkowska

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Changes in the induced sputum proteome during COPD exacerbations COPD加重期间诱导的痰蛋白组的变化

Molecular pathology and funct. genomics Pub Date : 2020-09-07 DOI: 10.1183/13993003.congress-2020.911

Ingvild Haaland, Marit Aardal, F. Berven, Marc Vaudel, R. Nielsen, M. Aanerud, J. Hardie, P. Bakke, T. Eagan

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Extracellular vesicle-derived muscle-specific miRNAs for the characterization of group B COPD patients 细胞外囊泡来源的肌肉特异性mirna用于B组COPD患者的表征

Molecular pathology and funct. genomics Pub Date : 2020-05-01 DOI: 10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a3998

T. Neri, S. Carpi, Beatrice Polini, D. Nieri, V. Scalise, C. Sanguinetti, Ilaria Passalacqua, R. Pedrinelli, P. Paggiaro, P. Nieri, A. Celi

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Late Breaking Abstract - Generating iPSC derived alveolar macrophages as a novel model for respiratory research 摘要-生成iPSC衍生的肺泡巨噬细胞作为呼吸研究的新模型

Molecular pathology and funct. genomics Pub Date : 2019-09-28 DOI: 10.1183/13993003.congress-2019.pa2343

Jessica Tilman, Eirini Koutsouraki, S. Humphreys, Ashley Barnes

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Differential macrophage activation in asthmatic sputum using U-BIOPRED transcriptomics 利用U-BIOPRED转录组学研究哮喘痰中巨噬细胞的差异活化

Molecular pathology and funct. genomics Pub Date : 2019-09-28 DOI: 10.1183/13993003.congress-2019.pa2337

A. Tiotiu, N. Kermani, P. Agapow, M. Saqi, Yike Guo, R. Djukanović, K. Chung, I. Adcock

{"title":"Differential macrophage activation in asthmatic sputum using U-BIOPRED transcriptomics","authors":"A. Tiotiu, N. Kermani, P. Agapow, M. Saqi, Yike Guo, R. Djukanović, K. Chung, I. Adcock","doi":"10.1183/13993003.congress-2019.pa2337","DOIUrl":"https://doi.org/10.1183/13993003.congress-2019.pa2337","url":null,"abstract":"Background: Macrophages (Mf) are important in controlling both innate and acquired immunity but their role in orchestrating the inflammatory response is severe asthma (SA) remains ill-defined. Aim: We investigated their role by analyzing the expression of Mf gene signatures in sputum transcriptomics from 104 asthma participants and 16 healthy volunteers (HV) of U-BIOPRED cohort. Methods: We used the previously-described transcriptome-analysis clusters (TACs) (Kuo CS, et al. ERJ 2017). Forty-nine differentially activated Mf gene signatures were studied using gene set variation analysis (GSVA) which gave an enrichment score (ES) across severity, TACs and macrophage M1/M2 polarization. ANOVA and adjusted p values were used for statistical analysis. Results: Mf activation was significantly suppressed in SA compared to HV. ES were down-regulated for most Mf subtypes in TAC1 (Th2 phenotype) and TAC2 (non-T2 inflammasome phenotype) vs TAC3 (non-T2 metabolic phenotype) and HV in favor of an up-regulation of immunoregulatory functions involved in Toll-like receptor and TNF receptor/NF-kB activation (all p Conclusions: Mf activation is suppressed in asthmatic compared to HV sputum. However, higher ES were seen for specific Mf subsets in eosinophilic and neutrophilic asthma compared with the greater Mf activation of most subtypes in TAC3 subjects. Targeting the specifically-activated Mf subtypes in different TACs may be an effective therapeutic approach.","PeriodicalId":224832,"journal":{"name":"Molecular pathology and funct. genomics","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116758262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Rare SERPINA1 allele Mwhitstable in patients with Alpha1-antitrypsin deficiency 罕见SERPINA1等位基因Mwhitstable与α - 1抗胰蛋白酶缺乏症患者的关系

Molecular pathology and funct. genomics Pub Date : 2019-09-28 DOI: 10.1183/13993003.congress-2019.pa4060

V. Barzon, A. Balderacchi, S. Ottaviani, A. Corsico, I. Ferrarotti

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Using transcriptomics to understand the potential functions of lung function associated gene, GPR126 in human airway smooth muscle cells 利用转录组学研究肺功能相关基因GPR126在人气道平滑肌细胞中的潜在功能

Molecular pathology and funct. genomics Pub Date : 2019-09-28 DOI: 10.1183/13993003.congress-2019.pa5410

R. Hall, M. Portelli, I. Hall, I. Sayers

{"title":"Using transcriptomics to understand the potential functions of lung function associated gene, GPR126 in human airway smooth muscle cells","authors":"R. Hall, M. Portelli, I. Hall, I. Sayers","doi":"10.1183/13993003.congress-2019.pa5410","DOIUrl":"https://doi.org/10.1183/13993003.congress-2019.pa5410","url":null,"abstract":"GPR126 lies on chromosome 6q24 and variants in this region are reproducibly associated with lung function and COPD in genome wide association studies (GWAS). Our aims were to determine the effects of GPR126 signalling in vitro and determine the downstream effects of GPR126 activation in human airway smooth muscle cells. GPR126 mRNA expression in the lung and airway cells was established using RNA-seq. To assess the downstream effects of GPR126 activation in human airway cells, we activated HASMs (n=4) with the GPR126 agonist, the stachel peptide in the presence and absence of PKA inhibitor, H89 to identify the PKA dependency of this response. Differential gene expression was analysed by RNA-seq. Pathway analysis was carried out with DAVID and Reactome. GPR126 mRNA is expressed in HASMs. Stachel peptide upregulated 206 genes at 4 hours (5%FDR) and 18 of these genes were still upregulated after pre-incubation with H89. Of the 308 downregulated genes, 7 were still downregulated after H89 treatment. A similar PKA dependency was observed at 24 hours for both induced and reduced gene expression in response to stachel peptide. H89 also influenced gene expression independent of the stachel response. Pathway analyses revealed that stachel activation upregulated genes in xenobiotic metabolism, HOX gene activation during differentiation, axon guidance and FOXO-mediated transcription. Downregulated genes are involved in MAPK signalling, cell death gene transcription and actin cytoskeleton regulation. We identify that HASMs respond to a GPR126 agonist with different transcriptome profiles at 4 and 24 hours and that this response is largely cAMP-PKA dependent.","PeriodicalId":224832,"journal":{"name":"Molecular pathology and funct. genomics","volume":"317 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115354078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0