The Histochemical Journal最新文献_第7页

Quantitative analysis of chondroitin sulphate retention by tannic acid during preparation of specimens for electron microscopy. 电镜标本制备过程中鞣酸对硫酸软骨素保留量的定量分析。

The Histochemical Journal Pub Date : 1995-06-01

D Levanon, H Stein

引用次数: 0

Immunohistochemical detection of secretoneurin, a novel neuropeptide endoproteolytically processed from secretogranin II, in normal human endocrine and neuronal tissues. 免疫组化检测正常人内分泌和神经组织中的分泌神经蛋白，一种由分泌颗粒蛋白II制成的新型内蛋白肽。

The Histochemical Journal Pub Date : 1995-06-01

K W Schmid, B Kunk, R Kirchmair, M Tötsch, W Böcker, R Fischer-Colbrie

{"title":"Immunohistochemical detection of secretoneurin, a novel neuropeptide endoproteolytically processed from secretogranin II, in normal human endocrine and neuronal tissues.","authors":"K W Schmid, B Kunk, R Kirchmair, M Tötsch, W Böcker, R Fischer-Colbrie","doi":"","DOIUrl":"","url":null,"abstract":"An antiserum raised against a synthetic peptide derived from the primary amino sequence of rat secretogranin II (chromogranin C) was used for immunological (quantitative radioimmunoassay analysis) and immunohistochemical studies of normal human endocrine and nervous tissues. This antibody recognized a novel and biologically active neuropeptide which was coined as secretoneurin. In endocrine tissues, secretoneurin was mainly co-localized with chromogranin A and B with some exceptions (e.g., parathyroid gland). Secretoneurin was demonstrated immunohistochemically in the adrenal medulla, thyroid C cells, TSH- and FSH/LH-producing cells of the anterior pituitary, A and B cells of pancreatic islets, in endocrine cells of the gastrointestinal tract and the bronchial mucosa, and the prostate. Immunoreactivity determined by radioimmunoassay analysis revealed high secretoneurin levels in the anterior and posterior pituitary and lower levels in pancreatic and thyroid tissue. A strong secretoneurin immunoreactivity was also found in ganglion cells of the submucosal and myenteric plexus of the gastrointestinal tract, and in ganglionic cells of dorsal root ganglia, peripheral nerves, and ganglion cells of the adrenal medulla. Thus, secretoneurin may serve as a useful marker of gangliocytic/neuronal differentiation.","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18566014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical localization of Na(+)-dependent glucose transporter in the rat digestive tract. 大鼠消化道Na(+)依赖性葡萄糖转运体的免疫组织化学定位。

The Histochemical Journal Pub Date : 1995-05-01

A Yoshida, K Takata, T Kasahara, T Aoyagi, S Saito, H Hirano

{"title":"Immunohistochemical localization of Na(+)-dependent glucose transporter in the rat digestive tract.","authors":"A Yoshida, K Takata, T Kasahara, T Aoyagi, S Saito, H Hirano","doi":"","DOIUrl":"","url":null,"abstract":"Glucose is actively absorbed in the intestine by the action of the Na(+)-dependent glucose transporter. Using an antibody against the rabbit intestinal Na(+)-dependent glucose transporter (SGLT1), we examined the localization of SGLT1 immunohistochemically along the rat digestive tract (oesophagus, stomach, duodenum, jejunum, ileum, colon and rectum). SGLT1 was detected in the small intestine (duodenum, jejunum and ileum), but not in the oesophagus, stomach, colon or rectum. SGLT1 was localized at the brush border of the absorptive epithelium cells in the small intestine. Electron microscopical examination showed that SGLT1 was localized at the apical plasma membrane of the absorptive epithelial cells. SGLT1 was not detected at the basolateral plasma membrane. Along the crypt-villus axis, all the absorptive epithelial cells in the villus were positive for SGLT1, whose amount increased from the bottom of the villus to its tip. On the other hand, cells in the crypts exhibited little or no staining for SGLT1. Goblet cells scattered throughout the intestinal epithelium were negative for SGLT1. These observations show that SGLT1 is specific to the apical plasma membrane of differentiated absorptive epithelial cells in the small intestine, and suggest that active uptake of glucose occur mainly in the absorptive epithelial cells in the small intestine.","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18662172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrarapid vacuum-microwave histoprocessing. 超快速真空微波组织处理。

The Histochemical Journal Pub Date : 1995-05-01

L P Kok, M E Boon

引用次数: 0

Substance P immunoreactivity in rat von Ebner's gland. 大鼠冯氏腺P物质免疫反应性。

The Histochemical Journal Pub Date : 1995-05-01

Y Oomori, Y Satoh, K Ishikawa, K Ono

{"title":"Substance P immunoreactivity in rat von Ebner's gland.","authors":"Y Oomori, Y Satoh, K Ishikawa, K Ono","doi":"","DOIUrl":"","url":null,"abstract":"The present immunohistochemical study revealed substance P-immunoreactive neuronal elements in the von Ebner's gland of rats. Immunoreactive ganglion cells were observed as single cells or groups of several immunoreactive ganglion cells among intra-lingual muscles, at the base of the vallate papillae and near the von Ebner's gland. Very numerous substance P-immunoreactive varicose nerve fibres ran closely associated with the serous cells and excretory duct cells, and were seen to run along blood vessels in the gland. Since substance P-immunoreactive ganglion cells were present near the glands, the immunoreactive varicose nerve fibres in the von Ebner's gland may be partly derived from the intra-lingual ganglion cells. These substance P-immunoreactive varicose nerve fibres may have an effect on the secretory activity of the serous cells and duct cells, and on the vasodilation of blood vessels of the von Ebner's gland. Actin immunoreactivity was seen in numerous myoepithelial cells embracing serous cells and duct cells, and in the smooth muscle cells of blood vessels of the gland. By using a double immunolabelling technique with anti-substance P and anti-actin sera, substance P-immunoreactive varicose nerve fibres were found to be in close contact with myoepithelial cells.","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18552333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. 溴脱氧尿苷:生物学和医学的诊断工具，第一部分:历史观点，组织化学方法和细胞动力学。

The Histochemical Journal Pub Date : 1995-05-01

F Dolbeare

引用次数: 0

Changes in thyrotroph and somatotroph cell populations induced by stimulation and inhibition of their secretory activity. 刺激和抑制促甲状腺和促生长细胞分泌活性引起的促甲状腺和促生长细胞群的变化。

The Histochemical Journal Pub Date : 1995-05-01

A I Torres, H A Pasolli, C A Maldonado, A Aoki

{"title":"Changes in thyrotroph and somatotroph cell populations induced by stimulation and inhibition of their secretory activity.","authors":"A I Torres, H A Pasolli, C A Maldonado, A Aoki","doi":"","DOIUrl":"","url":null,"abstract":"The populations of cells which produce immunoreactive growth hormone (GH) and thyroid stimulating hormone (TSH) in the rat pituitary gland do not occur in fixed percentages but vary greatly under different physiological and experimental conditions. These variations can be directly correlated to the levels of stimulation and/or inhibition of the specific secretory activity. In both types of cell, sustained stimulation with trophic hormones or blockage of the feedback mechanisms induces remarkable growth in the specific cell population. Conversely, the interruption or inhibition of the stimulus thwarted the hormonal secretion and caused a massive degeneration of redundant cells. The stimulation of both GH and TSH cells is accompanied by an enhanced secretory activity as judged by their higher concentrations in serum and hypertrophy of the cytoplasmic organelles involved in synthesis and intracellular processing of the hormones. By contrast, interruption of the stimulus is followed by a variable degree of disruption of the cytoplasmic organization, including a sizable degeneration of cells. In stimulated rats, the concentrations of both GH and TSH decreased significantly in pituitary tissue due to mobilization of the hormonal stores contained in secretory granules. On the other hand, the withdrawal of stimuli blocked the hormonal release; this is reflected by the accumulation of both hormones and secretory granules in pituitary tissue. The strict correlation between the size of the GH and TSH populations with stimulation and inhibition of hormonal secretory activity reported in this investigation further supports the critical role played by the cell renewal process in endocrine secretion.","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18661532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Periportal expression of the serine dehydratase gene in rat liver. 丝氨酸脱水酶基因在大鼠肝脏门静脉周围的表达。

The Histochemical Journal Pub Date : 1995-05-01

H Ogawa, S Kawamata

引用次数: 0

Poly-L-lysine-gold complexes used at different pH are probes for differential detection of glycosaminoglycans and phosphoproteins in the predentine and dentine of rat incisor. 不同pH下的聚赖氨酸-金配合物是大鼠前牙本质和牙本质中糖胺聚糖和磷蛋白的鉴别检测探针。

The Histochemical Journal Pub Date : 1995-05-01

M Goldberg, S Lécolle

{"title":"Poly-L-lysine-gold complexes used at different pH are probes for differential detection of glycosaminoglycans and phosphoproteins in the predentine and dentine of rat incisor.","authors":"M Goldberg, S Lécolle","doi":"","DOIUrl":"","url":null,"abstract":"At neutral pH, poly-L-lysine-gold complexes labelled the predentine extensively, whereas in dentine the number of gold complexes was reduced by half. Hyaluronidase pretreatment of the section at pH 6.8, prior to labelling, suppressed most of the staining in predentine and did not affect dentine. In contrast, alkaline phosphatase pretreatment at pH 9 enhanced the gold complex labelling in predentine and removed most of the labelling in dentine. This proves that at pH 7.2, the polyanions which are stained include a heterogeneous population of glycosaminoglycans, located in predentine, and phosphoproteins, visualized in dentine. At acidic pH levels (2.9 and 1.1), the number of scored gold complexes decreased, but the ratio between predentine and dentine labelling remained constant. Hyaluronidase pretreatment removed or firmly reduced the gold complex labelling both in predentine and dentine, whereas alkaline phosphatase pretreatment of the sections at pH 9 prior to labelling did not induce any change. This argues in favour of an increased specificity of polylysine-gold complex staining for glycosaminoglycans, stained at low pH in both predentine and dentine. Differential staining of glycosaminoglycans and phosphoproteins according to the pH provides a useful tool for studying the role played respectively by the two matrix components in dentine mineralization.","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18661535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of the butyrophilin gene, a milk fat globule membrane protein, is associated with the expression of the alpha S1casein gene. 亲丁酸蛋白基因(一种乳脂球膜蛋白)的表达与α s1酪蛋白基因的表达有关。

The Histochemical Journal Pub Date : 1995-05-01

A J Molenaar, S R Davis, L J Jack, R J Wilkins

{"title":"Expression of the butyrophilin gene, a milk fat globule membrane protein, is associated with the expression of the alpha S1casein gene.","authors":"A J Molenaar, S R Davis, L J Jack, R J Wilkins","doi":"","DOIUrl":"","url":null,"abstract":"Previous in situ hybridization studies from our laboratory have shown that expression of certain milk protein genes, e.g. alpha-lactalbumin, is very high in most parts of the mammary glands of sheep and cattle, while in other areas containing an abundance of fat globules it is virtually zero (Molenaar et al., 1992). One possible explanation is that some areas of the mammary gland are dedicated to protein synthesis and some to fat synthesis. To check this possibility, the cRNA for butyrophilin, a milk-fat globule membrane protein, and hence a putative marker of milk fat synthesis, was used as a probe in in situ hybridization studies. The results show quite clearly that the patterns of expression for this gene are similar, cell type for cell type, as those for milk protein genes such as alpha-lactalbumin and alpha S1casein. In addition, we found that butyrophilin gene expression more closely matches that of alpha S1casein than that of alpha-lactalbumin. If it is shown in the future that butyrophilin is indeed a marker for milk fat synthesis, then these results support the current assumption that fat and protein synthesis do occur in the same cell.","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18661534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0