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绿豆胰蛋白酶抑制剂Lys活力片段-牛胰蛋白酶复合物的晶体结构测定——分子置换法及3分辨率电子云密度图 绿豆胰蛋白酶抑制剂Lys活力片段-牛胰蛋白酶复合物的晶体结构测定——分子置换法及3分辨率电子云密度图
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-06-10 DOI: 10.1360/ZB1987-17-6-601
曾杰, 陈忠国, 李根培, 卢光莹, 卫新成, 唐有祺, 林光大, 张荣光, 宣建诚, 戚正武, 曹天钦
{"title":"绿豆胰蛋白酶抑制剂Lys活力片段-牛胰蛋白酶复合物的晶体结构测定——分子置换法及3分辨率电子云密度图","authors":"曾杰, 陈忠国, 李根培, 卢光莹, 卫新成, 唐有祺, 林光大, 张荣光, 宣建诚, 戚正武, 曹天钦","doi":"10.1360/ZB1987-17-6-601","DOIUrl":"https://doi.org/10.1360/ZB1987-17-6-601","url":null,"abstract":"本文以修正过的牛胰蛋白酶分子的坐标为模型,应用旋转函数法和平移函数法成功地求得了牛胰蛋白酶分子在绿豆胰蛋白酶抑制剂Lys活力片段(以下简称MBILF)-牛胰蛋白酶(以下简称BTRY)复合物晶体晶胞中的取向和位置。从正确放置于MBILF-BTRY复合物晶胞中的BTRY分子的坐标出发,用Sim权重方法算得的一套相角作为MBILF-BTRY复合物的相角,以2丨F 0 |—丨F c 丨为系数所得到的3分辨率的电子密度图中,除了投入计算的BTRY分子本身的电子密度外,在BTRY的活性部位附近可以清晰地看到MBILF分子所对应的电子密度及其边界,抑制剂片段的线度约为15×15×25。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123867075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The complete nucleotide sequence of the cloned DNA of hepatitis B virus subtype adr in pADR-1. 乙型肝炎病毒adr亚型在pADR-1中的克隆DNA的全核苷酸序列。
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-05-10 DOI: 10.1360/YB1987-30-5-507
Gan Rb, Churchouse Mj, Shen Lp, Qian Sw, Liu Zp
{"title":"The complete nucleotide sequence of the cloned DNA of hepatitis B virus subtype adr in pADR-1.","authors":"Gan Rb, Churchouse Mj, Shen Lp, Qian Sw, Liu Zp","doi":"10.1360/YB1987-30-5-507","DOIUrl":"https://doi.org/10.1360/YB1987-30-5-507","url":null,"abstract":"The complete nucleotide sequence of the cloned hepatitis B virus DNA subtype adr in pADR-1 was determined by Maxam and Gilbert's method. It is 3215 base pairs in size, which is 27 bp longer than the sequence of the adr pHBr330, as reported by Ono et al. The nucleotide difference between pADR-1 and adr pHBr330 is about 2% while those between pADR-1 and adw as well as ayw are 9.3% and 9.7% respectively. In this paper, the heterogeneity and homogeneity of the S gene, the C gene and the other coding regions in pADR-1 and in the other subtypes are compared and discussed.","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116243953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
THERMAL STRUCTURE OF THE CRUST AND UPPER MANTLE OF LIAOHE RIFT BASIN, NORTH CHINA 辽河裂谷盆地地壳和上地幔热结构
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-05-10 DOI: 10.1360/YB1987-30-5-553
W. Jiyang, Wang Ji-an
{"title":"THERMAL STRUCTURE OF THE CRUST AND UPPER MANTLE OF LIAOHE RIFT BASIN, NORTH CHINA","authors":"W. Jiyang, Wang Ji-an","doi":"10.1360/YB1987-30-5-553","DOIUrl":"https://doi.org/10.1360/YB1987-30-5-553","url":null,"abstract":"On the basis of analysing terrestrial heat flow and mantle heat flow values, the crustal temperatureat depth was calculated for Liaohe Rift Basin in North China, and a conceptual model of thermal struc-ture of the crust and upper mantle of the Basin has been suggested. Results indicate that Liaohe Basinis characterized by a relatively high mantle heat flow (0.98 HFU) but low crustal heat flow (0.57HFU),both caused by upwelling of the asthenosphere and crustal thinning during rifting process in early Eo-gene in thc region studied. This sort of thermal structure of the crust and upper mantle seems to beinherent in the region in between tectonically stable and active areas and has been considered to be theproduct of the history of geological evolution of the Basin since Mesozoic era.","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116443925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
记甘肃和政新发现的大唇犀类化石——兼论中国“对鼻角犀”的性质 记甘肃和政新发现的大唇犀类化石——兼论中国“对鼻角犀”的性质
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-05-10 DOI: 10.1360/ZB1987-17-5-545
邱占祥, 谢俊义, 阎德发
{"title":"记甘肃和政新发现的大唇犀类化石——兼论中国“对鼻角犀”的性质","authors":"邱占祥, 谢俊义, 阎德发","doi":"10.1360/ZB1987-17-5-545","DOIUrl":"https://doi.org/10.1360/ZB1987-17-5-545","url":null,"abstract":"本文记述了甘肃和政,晚中新世地层中发现的一个大唇犀类的头骨— Acerorhinus hezhengensis sp。nov。笔者详细地讨论了中国“对鼻角犀”的性质,指出它和北美真正的对鼻角犀相去甚远。笔者不同意Heissig把“对鼻角犀”作为大唇犀一个亚属的意见,而把它视为一个独立的属,其属名采用Kretzoi 1942年所创的 Acerorhinus ,它和大唇犀一起组成无角犀亚科中的大唇犀族。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134006397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Z-和E-β-苯乙烯基溴化镁的生成机理及其与三苯基氯甲烷的反应 Z-和E-β-苯乙烯基溴化镁的生成机理及其与三苯基氯甲烷的反应
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-05-10 DOI: 10.1360/ZB1987-17-5-470
刘有成, 党海山
{"title":"Z-和E-β-苯乙烯基溴化镁的生成机理及其与三苯基氯甲烷的反应","authors":"刘有成, 党海山","doi":"10.1360/ZB1987-17-5-470","DOIUrl":"https://doi.org/10.1360/ZB1987-17-5-470","url":null,"abstract":"z一和E-β-溴苯乙烯与镁在THF中反应生成构型保持的格氏试剂和部分异构化的格氏试剂。副产物有苯乙烯、苯乙炔和E,E-1,4-二苯基丁二烯-1,3。用 1 Hnmr检测反应过程时,发现Z-β-溴苯乙烯与镁在THF-d_8中反应的产物Z-β-苯乙烯基溴化镁、E-β-苯乙烯-d和E,E-1,4-二苯基丁二烯-1,3出现CIDNP效应。根据这两个格氏试剂与二氧化碳反应所得产物和CIDNP效应,本文提出反应是通过镁转移单电子给β-溴苯乙烯的自由基机理进行的。研究题目格氏试剂与三苯基氯甲烷的反应,进一步证实了由单电子转移生成的烯基格氏试剂自由基正离子可以发生β-氢转移。由自由基二聚和交叉结合的速率的不同,文中解释了苯基取代氯甲烷与烯基格氏试剂反应时所得的AA,AB和BB型产物的分布。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132174448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CPS 1 cDNA克隆及大鼠肝癌癌变过程中CPS 1 mRNA量的变化 CPS 1 cDNA克隆及大鼠肝癌癌变过程中CPS 1 mRNA量的变化
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-05-10 DOI: 10.1360/ZB1987-17-5-518
吴石君, 李士谔, 张海澜, 罗坚, 于跃
{"title":"CPS 1 cDNA克隆及大鼠肝癌癌变过程中CPS 1 mRNA量的变化","authors":"吴石君, 李士谔, 张海澜, 罗坚, 于跃","doi":"10.1360/ZB1987-17-5-518","DOIUrl":"https://doi.org/10.1360/ZB1987-17-5-518","url":null,"abstract":"本研究以CPS 1 mRNA为模板合成cDNA,与质粒pBR 322 DNA重组,经选择杂交法筛选获得CPS 1 cDNA克隆。以 32 P标记cDNA作为探针与二乙基亚硝胺(DENA)诱发大鼠肝癌过程中不同病变肝中提取的总RNA及poly(A) + RNA杂交。结果发现,CPS 1 mRNA的量随肝细胞癌变程度加深而递减,说明肝癌癌变过程中,CPS 1 基因表达的异常可能发生在转录水平。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"418 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124256921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
二氢和四氢-2,4-二取代-1,5-苯并二氮杂的光谱和立体化学 二氢和四氢-2,4-二取代-1,5-苯并二氮杂的光谱和立体化学
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-04-10 DOI: 10.1360/ZB1987-17-4-339
吕迎潮, 刘平, 金声, 邢其毅迎潮
{"title":"二氢和四氢-2,4-二取代-1,5-苯并二氮杂的光谱和立体化学","authors":"吕迎潮, 刘平, 金声, 邢其毅迎潮","doi":"10.1360/ZB1987-17-4-339","DOIUrl":"https://doi.org/10.1360/ZB1987-17-4-339","url":null,"abstract":"本文制备了一系列2,4-二取代-1,5-苯并二氮杂草,研究了它们的光谱和立体结构。二氢化物具有船式构象,而且2位取代基可以朝向“船头”的下方,处于与4位取代基近似地为五原子重叠的地位,但分子仍然是稳定的,这种现象较少见。二氢化物用硼氢化钠还原,生成含量相近的两种四氢化物,其中高熔点异构体确定为顺式椅式构象而低熔点异构体具有反式扭船式构象。这一结果纠正了福岛清吾等关于这两种异构体的不正确的指认。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125671416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
两个具有松散配位的三核钼簇合物{Mo 3 (μ 3 —S)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (C 3 H 3 ON)}·CH 3 CN(I){Mo 3 (μ 3 —O)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (Cμ 3 Hμ 3 ON)}(II)的合成和晶体结构 两个具有松散配位的三核钼簇合物{Mo 3 (μ 3 —S)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (C 3 H 3 ON)}·CH 3 CN(I){Mo 3 (μ 3 —O)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (Cμ 3 Hμ 3 ON)}(II)的合成和晶体结构
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-04-10 DOI: 10.1360/ZB1987-17-4-358
卢绍芳, 商茂虞, 黄建全, 黄金陵, 卢嘉锡
{"title":"两个具有松散配位的三核钼簇合物{Mo 3 (μ 3 —S)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (C 3 H 3 ON)}·CH 3 CN(I){Mo 3 (μ 3 —O)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (Cμ 3 Hμ 3 ON)}(II)的合成和晶体结构","authors":"卢绍芳, 商茂虞, 黄建全, 黄金陵, 卢嘉锡","doi":"10.1360/ZB1987-17-4-358","DOIUrl":"https://doi.org/10.1360/ZB1987-17-4-358","url":null,"abstract":"本文报道了两个具有松散配位的三核钼簇合物{Mo 3 (μ 3 —S)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (C 3 H 3 ON)}·CH 3 CN(Ⅰ){Mo 3 (μ 3 —O)(μ—S) 3 [S 2 P(OEt) 2 ] 4 (Cμ 3 Hμ 3 ON)}(Ⅱ)的合成和晶体结构。簇合物(Ⅰ)属单斜晶系,空间群为P2 2 /n,晶胞参数为 a =16.579(3), b =16.959(2), c =16.867(2); β =94.44(1)°; v =4728 3; Z =4; D c =1.778g·cm -3 ,用Patterson法解出结构,最终偏离因子 R =0.045。簇合物(Ⅱ)属三斜晶系,空间群为 P 1,晶胞参数为 a =13.175(4), b =13.372(6), c =16.656(3); α =117.87(4)°, β =60.09(3)°, γ =109.03(3)°; ν =2231 3; Z =2; D c =1.798g·cm -3 ,用直接法解出结构,最终偏离因子R=0.066。两个簇合物均为以具有一个松散配位为特征的单帽三核簇,而且松散配位上均被一嘿唑五员环所占据,其主要差别在于簇合物(Ⅰ)的簇胳为Mo 3 S 4 ,而簇合物(Ⅱ)则为一Mo 3 OS 3 ·文中对这一“松散”配位的三核钼簇合物的活泼性进行了讨论。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114181478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
试论南黄海古生代到早-中三叠世古地理、构造型式和可能的油捕类型 试论南黄海古生代到早-中三叠世古地理、构造型式和可能的油捕类型
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-03-10 DOI: 10.1360/ZB1987-17-3-311
陈颐亨
{"title":"试论南黄海古生代到早-中三叠世古地理、构造型式和可能的油捕类型","authors":"陈颐亨","doi":"10.1360/ZB1987-17-3-311","DOIUrl":"https://doi.org/10.1360/ZB1987-17-3-311","url":null,"abstract":"本文根据南黄海周边地区(中国江苏和朝鲜南部)地质调查与海区少量钻井及海岛踏勘资料,编制了古生代至早-中三叠世古地理图,分析了盆地演化及其特有的构造风格,探讨了可能的构造圈闭型式和油捕类型,并指出今后找油方向。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123092909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A COMPARISON OF THE PRIMARY STRUCTURES OF LACTATE DEHYDROGENASE ISOZYMES M 4 FROM GIANT PANDA, RED PANDA,BLACK BEAR AND DOG 大熊猫、小熊猫、黑熊和狗乳酸脱氢酶同工酶m4初级结构的比较
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-03-10 DOI: 10.1360/YB1987-30-3-270
Liang Songping, Zhang Longxiang
{"title":"A COMPARISON OF THE PRIMARY STRUCTURES OF LACTATE DEHYDROGENASE ISOZYMES M 4 FROM GIANT PANDA, RED PANDA,BLACK BEAR AND DOG","authors":"Liang Songping, Zhang Longxiang","doi":"10.1360/YB1987-30-3-270","DOIUrl":"https://doi.org/10.1360/YB1987-30-3-270","url":null,"abstract":"Lactate dehydrogenase isozymes M_4 have been isolated and purified from red panda (Ailurus fulgens), \u0000black bear (Selenarctos thibetanus) and dog (Canis familiars) by affinity chromatography and compared \u0000with that from giant panda (Ailuropoda melanoleuca). Experimental results have shown that the N-ter- \u0000mini, C-termini and the molecular weights of LDH-M subunits of red panda, black bear and dog are \u0000the same as those of the LDH-M subunit of giant panda. Analysis and comparison of HPLC peptide \u0000maps from the tryptic digests of the isozymes of red panda, black bear and dog have shown that most \u0000of their peptide fragments had the same retention time and amino acid composition as the corresponding \u0000peptide fragments from giant panda. Fragments with different retention times and/or amino acid compo- \u0000sitions were sequentced. Careful examination of those variant amino acid residues demonstrated clearly \u0000that the primary structure of giant panda LDH-M subunit is unique and it appears that the giant panda \u0000might be classified as an independent family.","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114983744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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