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癌变原理研究——IX.大鼠肝癌癌变过程中细胞增殖酶和组织特异酶基因表达的相互变化 癌变原理研究——IX.大鼠肝癌癌变过程中细胞增殖酶和组织特异酶基因表达的相互变化
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-11-10 DOI: 10.1360/ZB1987-17-11-1198
李士谔 吴石君
{"title":"癌变原理研究——IX.大鼠肝癌癌变过程中细胞增殖酶和组织特异酶基因表达的相互变化","authors":"李士谔 吴石君","doi":"10.1360/ZB1987-17-11-1198","DOIUrl":"https://doi.org/10.1360/ZB1987-17-11-1198","url":null,"abstract":"以CPS 1 ,OCT,ACT cDNA克隆片段为探针与二乙基亚硝胺诱发大鼠肝癌癌变过程不同病变肝组织的poly(A) + -RNA进行斑点杂交及Northern印迹杂交。结果表明:组织特异酶CPS 1 ,OCT mRNA量降低,而细胞增殖酶ACT mRNA量升高。这些mRNA量的变化都随病变程度而加深。CPS 1 mRNA分子略大于28S,OCT mRNA约为15S,ACT mRNA分子约为35S。癌变过程中CPS 1 ,OCT以及ACT mRNA量的相互变化与不同癌变肝组织酶活性的相互变化呈相关关系。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114782128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
B淋巴细胞造血调控活性的研究——II.增殖放大因子的分离提纯 B淋巴细胞造血调控活性的研究——II.增殖放大因子的分离提纯
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-10-10 DOI: 10.1360/ZB1987-17-10-1085
姚志建, 江飞子, 黎燕, 郭燕捷, 张明伟, 徐元基, 唐佩弦
{"title":"B淋巴细胞造血调控活性的研究——II.增殖放大因子的分离提纯","authors":"姚志建, 江飞子, 黎燕, 郭燕捷, 张明伟, 徐元基, 唐佩弦","doi":"10.1360/ZB1987-17-10-1085","DOIUrl":"https://doi.org/10.1360/ZB1987-17-10-1085","url":null,"abstract":"本文首次报道用亲和层析和高效液相色谱等技术由B淋巴瘤M12.4.1的培养上清中分离到一种新的造血调控因子——增殖放大因子(PAF)。PAF在SDSPAGE电泳上呈现分子量为31000 D的单一谱带,比活性较M12.4.1培养上清提高了20000倍,为B淋巴细胞的造血调控作用提供了直接的证据。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131008952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
牛心线粒体F 1 -ATP酶亚基的相互作用和化学计量关系 牛心线粒体F 1 -ATP酶亚基的相互作用和化学计量关系
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-10-10 DOI: 10.1360/ZB1987-17-10-1051
刘树森
{"title":"牛心线粒体F 1 -ATP酶亚基的相互作用和化学计量关系","authors":"刘树森","doi":"10.1360/ZB1987-17-10-1051","DOIUrl":"https://doi.org/10.1360/ZB1987-17-10-1051","url":null,"abstract":"本文证明牛心线粒体F 1 与[ 14 C]-NBD-Cl在黑暗中保温,生成O-NBD-F 1 ,其NBD/F 1 标记率为1;ATP酶活力抑制率为98％,但可被2mmol/L DTT恢复,提示F 1 酪氨酸残基被标记。O-NBD-F 1 经光照后NBD基团转移到氨基上,生成N-NBD-F 1 ;其NBD/F 1 标记率为0.825,酶活力抑制率为83％。N-NRD-F 1 脲聚丙烯酰胺凝胶电泳分析表明,63.4％放射活性标记在F 1 的β亚基,但纯化的β基中放射活性为20％。结果证明F 1 含3个β亚基,亚基之间和位点之间有相互调控作用,从而支持F 1 酶催化反应中三位点模型(Boyer,1977年)。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127154296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
激光诱导荧光法检测火焰温度和OH,C 2 ,CH基浓度的二维分布 激光诱导荧光法检测火焰温度和OH,C 2 ,CH基浓度的二维分布
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-10-10 DOI: 10.1360/ZB1987-17-10-1041
倪图强, 聂绪建, 俞书勤, 孔繁敖
{"title":"激光诱导荧光法检测火焰温度和OH,C 2 ,CH基浓度的二维分布","authors":"倪图强, 聂绪建, 俞书勤, 孔繁敖","doi":"10.1360/ZB1987-17-10-1041","DOIUrl":"https://doi.org/10.1360/ZB1987-17-10-1041","url":null,"abstract":"本文通过对预混的,微富燃料的饱和烃/空气火焰中各部分的OH(A 2 Σ + ←→X 2 Π ),C 2 (A 3 Π←→X 3 Π )和CH(B 2 Σ←X 2 Π ),(A 2 Δ →X 2 Π )的激光诱导荧光信号进行逐点检测,首次获得了OH(X),C 2 (X)和CH(X)等自由基在火焰中央纵剖面上的二维等浓度线分布图。OH(X)基在外焰边缘上分布最浓,是由H和O原子反应所产生的。C 2 主要来自热解,而CH基是由小自由基和含氧化合物反应生成的,分布在内锥面附近及锥顶区,重燃料的C 2 和CH分布区既浓又广,并逐步过渡到大量形成碳粒的黄光区。通过逐点测量OH(A←→X)的激光诱导荧光谱,首次测得了火焰纵剖面上的二维等温图。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127261159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
硫代磷(或膦)酰-双-异硫氰酸酯与胺加成反应的研究II 硫代磷(或膦)酰-双-异硫氰酸酯与胺加成反应的研究II
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-10-10 DOI: 10.1360/ZB1987-17-10-1031
胡式贤 陈茹玉
{"title":"硫代磷(或膦)酰-双-异硫氰酸酯与胺加成反应的研究II","authors":"胡式贤 陈茹玉","doi":"10.1360/ZB1987-17-10-1031","DOIUrl":"https://doi.org/10.1360/ZB1987-17-10-1031","url":null,"abstract":"本文报道了硫代磷(或膦)酰-双-异硫氰酸酯与胺的反应,合成了通式为Ⅰ,Ⅱ,Ⅲ,Ⅳ的四系列新的有机磷化合物,所有化合物的结构经元素分析、红外吸收光谱和~1H核磁共振谱证明,并且还以典型化合物为例,测定了其质谱或X光衍射,进一步确认了化合物的结构。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"627 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133202004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
多肽研究——I.α-人心房肽(α-hANP)带保护基的片段的合成 多肽研究——I.α-人心房肽(α-hANP)带保护基的片段的合成
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-09-10 DOI: 10.1360/ZB1987-17-9-969
蔡孟深, 彭师奇, 程铁明, 戴敦华, 王少农, 李金祥, 张京莉, 丛阳, 梁伟升, 王融, 杨生生, 陈双环, 吴小东, 张艾卓, 张肇康
{"title":"多肽研究——I.α-人心房肽(α-hANP)带保护基的片段的合成","authors":"蔡孟深, 彭师奇, 程铁明, 戴敦华, 王少农, 李金祥, 张京莉, 丛阳, 梁伟升, 王融, 杨生生, 陈双环, 吴小东, 张艾卓, 张肇康","doi":"10.1360/ZB1987-17-9-969","DOIUrl":"https://doi.org/10.1360/ZB1987-17-9-969","url":null,"abstract":"本文报道了与α-人心房肽(α-hANP)的A_1— A 4 四肽及 A 5 — A 10 , A 11 — A 16 ,A 17 — A 22 , A 23 — A 28 四个六肽片段对应的带保护基片段的液相法合成。在有些肽段的合成中,比较了混合酸酐法和活泼酯法所得的结果。在另一些肽段的合成中,观察了反应温度、催化剂的碱性及反应时间对产物产率的影响。上述带保护基的片段均由快原子轰击质谱测定氨基酸顺序,确认结构准确无误。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126246083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ROLE OF CALCIUM IN ELECTRO-ACUPUNCTURE ANALGESIA AND THE DEVELOPMENTS OF ANALGESIC TOLERANCE TO ELECTROACUPUNTURE AND MORPHINE 钙在电针镇痛中的作用及对电针和吗啡镇痛耐受的进展
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-09-10 DOI: 10.1360/YB1987-30-9-974
Zhang Zuxuan, Zhang Yuanzhen, J. Shuping, Lu Xuejian, Yu Rui-rong, Wang Xinguang, Rongsan
{"title":"ROLE OF CALCIUM IN ELECTRO-ACUPUNCTURE ANALGESIA AND THE DEVELOPMENTS OF ANALGESIC TOLERANCE TO ELECTROACUPUNTURE AND MORPHINE","authors":"Zhang Zuxuan, Zhang Yuanzhen, J. Shuping, Lu Xuejian, Yu Rui-rong, Wang Xinguang, Rongsan","doi":"10.1360/YB1987-30-9-974","DOIUrl":"https://doi.org/10.1360/YB1987-30-9-974","url":null,"abstract":"Role of brain Ca~(2+) in electro-acupuncture analgesia and the development of analgesic tolerance to electro-acupuncture and morphine were studied. At the same time, the inhibition by protein synthesis inhibiturs of the development of analgesic tolerance to electro-acupuncture was observed. The results showed that like morphine tolerance, the brain Ca~(2+) and cAMP levels in mice were enhanced with the development of analgesic tolerance to electro-acupuncture. After treatment with protein synthesis inhibitors anisomycin, actinomycin or cycloheximide the development of analgesic tolerance to electro-acupuncture was inhibited, and concurrently, the brain Ca~(2+) and cAMP levels in the animals greatly reduced. From the changes of brain Ca~(2+) and cAMP levels, the analgesic effects by electro-acupuncture, morphine and lanthanides seem to be very similar and share a mutual ion basis and the mechanism of action. So does the development of analgesic tolerance to electro-acupuncture and morphine. These findings also suggest that the inhibition induced by the inhibitors of the analgesic tolerance to electro-acupuncture and morphine may be related to synthesis of new peptides or RNA in brain.","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122193891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
H+O 2 体系在低碰撞能下的动力学研究 H+O 2 体系在低碰撞能下的动力学研究
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-09-10 DOI: 10.1360/ZB1987-17-9-920
居冠之, 冯大诚, 蔡政亭, 邓从豪
{"title":"H+O 2 体系在低碰撞能下的动力学研究","authors":"居冠之, 冯大诚, 蔡政亭, 邓从豪","doi":"10.1360/ZB1987-17-9-920","DOIUrl":"https://doi.org/10.1360/ZB1987-17-9-920","url":null,"abstract":"本文用准经典轨迹理论,模拟了H( 2 S)原子与O 2 ( 3 Σ g - )分子在ab initio势能面上的反应碰撞过程,研究了该反应体系在低碰撞能 E =0.3eV,O 2 的量子态v(振动量子数)=2, j (转动量子数)=1,20,40,60,80,100时的动力学行为。计算结果表明:(1)发生反应的最大碰撞参数 b max 为4.5 a o ,(2)产生最大反应几率的碰撞参数b随j的增加而减小,(3)反应截面随j单调增加,(4)对于小b高j时的非弹性碰撞,产物O 2 的振动量子数 v ′和转动量子数 j ′均明显地下降,而对于反应碰撞,产物OH( 2 Π)多处于基振动态( v ′=0),且随j的增加,产物OH处于振动激发态( v ′>0)的几率明显增加,当 j =100时,甚至出现振动态分布倒反现象。此外,还绘制了部分典型的碰撞轨迹图。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126049058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
直链共轭高分子的分子轨道理论——III.二维共轭体系本征方程的分解问题 直链共轭高分子的分子轨道理论——III.二维共轭体系本征方程的分解问题
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-09-10 DOI: 10.1360/ZB1987-17-9-927
杨忠志, 叶元杰, 唐敖庆
{"title":"直链共轭高分子的分子轨道理论——III.二维共轭体系本征方程的分解问题","authors":"杨忠志, 叶元杰, 唐敖庆","doi":"10.1360/ZB1987-17-9-927","DOIUrl":"https://doi.org/10.1360/ZB1987-17-9-927","url":null,"abstract":"","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122763951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
α-因子指导下β-hCG在酵母中的合成和分泌 α-因子指导下β-hCG在酵母中的合成和分泌
Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences Pub Date : 1987-09-10 DOI: 10.1360/ZB1987-17-9-947
唐鹰, 匡达人, 吴瑞
{"title":"α-因子指导下β-hCG在酵母中的合成和分泌","authors":"唐鹰, 匡达人, 吴瑞","doi":"10.1360/ZB1987-17-9-947","DOIUrl":"https://doi.org/10.1360/ZB1987-17-9-947","url":null,"abstract":"本文利用酵母接合因子α基因(MFα-1)的启动子、前导肽顺序(α- factor leader sequence )以及2μm质粒复制起始顺序(2μori)构建了一组酵母-大肠杆菌穿梭分泌型表达载体(pYNA,pYNB)。将人绒毛膜促性腺激素β-亚单位(β-hCG)基因(145肽顺序)融合于这一前导肽顺序的3′端,在酵母( Saccharomyces cerevisiae )中得到表达和产物分泌。表达产物与天然β-hCG全抗体及单克隆抗体都有免疫反应。用β-hCG放射免疫双抗试剂盒测到每升培养物含有45μg产物。对这一产物的部分特性做了初步鉴定。","PeriodicalId":223601,"journal":{"name":"Science in China Series B-Chemistry, Biological, Agricultural, Medical & Earth Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1987-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124298810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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