{"title":"CHROMOSOMAL LOCATIONS OF mcr-1 IN Klebsiella pneumoniae AND Enterobacter cloacae FROM DOGS","authors":"Ming-Huang Chang, Guanjun Chen, D. Lo","doi":"10.1142/S168264851972003X","DOIUrl":"https://doi.org/10.1142/S168264851972003X","url":null,"abstract":"In November 2015, the emergence of a novel plasmid-mediated colistin resistance mechanism was described. So far, there are only two relevant published reports focused solely on Escherichia coli and Salmonella in Taiwan. This paper describes the emergence of colistin-resistant Klebsiella pneumoniae and Enterobacter cloacae clinical isolates harboring mcr-1 on chromosomes in Taiwan. All four mcr-1-positive isolates were from 63 Klebsiella spp., and the isolated Enterobacter spp. were from diseased dogs and cats at Veterinary Teaching Hospital, National Chiayi University. These four isolates were resistant to colistin, amoxicillin, doxycycline and oxytetracycline, and all mcr-1 genes were located on the chromosome, without any flanking ISApl1 or other insertion sequences. The findings suggest that, in addition to food animals and humans, companion animals can serve as reservoirs of mcr-1, adding another layer of complexity to the rapidly evolving epidemiology of plasmid-mediated colistin resistance in the community. Hence we consider it essential to continue to survey resistance to colistin in these bacteria. Continuous microbiological and molecular surveillance is necessary to assist in early detection and minimize the dissemination of mcr-1.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74925623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jia-Ling Yang, Lenny Hao-Che Yen, W. Yen, Fun-In Wang
{"title":"A SUBCLINICAL BLUETONGUE VIRUS INFECTION IN RUMINANTS WITH THREE UNIQUE AMINO ACID VARIATIONS ON VP7 CORE PROTEIN OF TAIWAN ISOLATES","authors":"Jia-Ling Yang, Lenny Hao-Che Yen, W. Yen, Fun-In Wang","doi":"10.1142/S168264851950001X","DOIUrl":"https://doi.org/10.1142/S168264851950001X","url":null,"abstract":"Bluetongue is an arthropod-borne disease in domestic and wild ruminants caused by bluetongue virus (BTV), and it leads to great economic loss worldwide. Previous studies showed that BTV in ruminants in Taiwan was often subclinical infection. The aim of this study was to determine the current status (years 2016–2017) of BTV infection in ruminants in Taiwan, to compare it to the results of a large-scale study conducted in the year 2003, and to investigate whether new viral strains exist. Competitive ELISA tests of serum samples for anti-BTV-VP7 group-specific antibody revealed seropositive rates of 26.7% in cattle by head, similar to 32.7% in the year 2003, suggestive of a BTV-vector-host (cattle) dynamic balance. In goats, the seropositive rate was 18.6%, slightly increased from 8.2% in the year 2003, suggestive of a slow but active infection taking place. This notion was supported by the detection of VP1 gene nucleic acid from whole blood in six out of 29 seropositive goats by reverse transcription–polymerase chain reaction. However, no new virus strain was isolated from embryonating chicken embryos (ECEs) inoculation. Alignment of VP7 amino acid sequences revealed that Taiwan and Japan isolates possessed three specific amino acids on sites No. 82 (arginine), No. 328 (aspartate), and No. 336 (glutamine), which are different from many countries. In a three-dimensional model, these amino acids were located closely on the middle lateral surface of VP7 trimers. Since VP7 is a major outer protein engaged in entry into insect cells and a strong T cell response inducer, these differences likely indicate the result of positive selection of local vectors and hosts in Taiwan.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79968987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chung-Chao Chen, Shu-Fang Yang, Shei-Wen Lee, Heng-Leng Yang, F. Hsieh, Kwo-Chen Hu, S. Liang
{"title":"DISCOSPONDYLITIS IN A 7-MONTH-OLD FRENCH BULLDOG: A CASE REPORT","authors":"Chung-Chao Chen, Shu-Fang Yang, Shei-Wen Lee, Heng-Leng Yang, F. Hsieh, Kwo-Chen Hu, S. Liang","doi":"10.1142/S1682648519720016","DOIUrl":"https://doi.org/10.1142/S1682648519720016","url":null,"abstract":"A 7-month-old, intact, male French bulldog presented for paraplegia for two days, and persistent fever and diarrhea for seven days. Complete blood count and biochemical profiles were within normal limits. Radiography and computed tomography (CT) revealed destructive endplate lesions between T11 and T12. Surgery of intervertebral disc (IVD) curettage was performed because the dog was beginning to lose deep pain perception. Staphylococcus epidermis was isolated from blood culture and specimens of the IVD. After surgery, intravenous and oral antibiotics were continued for three weeks. The dog regained ambulatory status one month after surgery. Diagnosis of discospondylitis of T11 and T12 was confirmed according to CT and positive bacteria culture. To our knowledge, this case is the youngest dog confirmed with discospondylitis managed successfully, and the first case report of discospondylitis in a French bulldog. Aggressive surgical treatment is highly recommended in young dogs with rapidly progressive neurologic deficits from discospondylitis.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90122297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Lo, Yu-Ching Feng, Yi-Ping Yang, J. Liao, Shih-Chieh Chang
{"title":"WITH SURGICAL REMOVAL OR ADJUVANT CHEMOTHERAPY INCREASE THE TWO-YEAR SURVIVAL AND ASSOCIATED CLINICOPATHOLOGIC FACTORS IN CATS WITH MAMMARY CARCINOMAS","authors":"T. Lo, Yu-Ching Feng, Yi-Ping Yang, J. Liao, Shih-Chieh Chang","doi":"10.1142/S1682648519500021","DOIUrl":"https://doi.org/10.1142/S1682648519500021","url":null,"abstract":"Due to the aggressive biological behavior, a large number of studies attempt to identify the prognostic indicators for feline mammary carcinoma (FMC). In this study, we retrospectively identified the prognostic indicators and evaluated the effect of surgery with or without adjuvant chemotherapy on 47 cats with FMCs. Over a two-year follow-up period, surgical removal was significantly associated with greater overall survival time (OST). In cats that underwent surgical treatment, median OST was longer in cats that underwent surgery with adjuvant chemotherapy (540 days) than cats that underwent surgery without chemotherapy (398 days). In addition, cats with FMCs in early stage (I or II) had longer OST than cats in advanced stages. Further, through multivariate analyses, the histological grade was found to be significantly associated with a survival of two years. Cats with FMCs at high grade were most likely to have a mean or median OST of less than one year. In summary, stage, grade and the size of tumor were all prognostic factors, in which histological grade was found to be the only significant factor in cats with mammary carcinomas through multivariate analysis.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89370084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jung-Chin Chang, Fang-Yi Tsai, Yu-Shing Lee, R. Wang, Ju-Pai Kao, H. Chiou, J. Liao
{"title":"CASE REPORT: LIPOSARCOMA WITH MICROVASCULAR PROLIFERATION IN A COCKATIEL (Nymphicus hollandicus)","authors":"Jung-Chin Chang, Fang-Yi Tsai, Yu-Shing Lee, R. Wang, Ju-Pai Kao, H. Chiou, J. Liao","doi":"10.1142/S1682648519720028","DOIUrl":"https://doi.org/10.1142/S1682648519720028","url":null,"abstract":"A five-year-old female cockatiel weighing 117 g was presented with a fast-growing mass beside the uropygial gland. Excisional biopsy was performed and the mass measured [Formula: see text][Formula: see text]cm in size and weighed 30.6 g. On the surface of cut sections, the mass was yellow-brown with white or yellow colloidal substances and red exudate. Histopathology showed that the tumor mass was covered by the skin and located in the deep dermis and hypodermis. The tumor consisted of abundant vascular adipose tissue and lipoblasts with intracytoplasmic lipid droplets, which varied in size. Also, small, well-differentiated blood vessels, with varied degrees of congestion and dilation, were observed within the tumor. Histochemically, staining with Oil red O produced a positive reaction in which the lipid droplets presented a reddish color. Immunohistochemistry produced positive staining for Desmin and successfully marked the muscular layers of blood vessels. On the basis of these results, a rare case of liposarcoma with microvascular proliferation adjacent to the uropygial gland was diagnosed in a cockatiel.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83989712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"AVIAN INNATE IMMUNITY WITH AN EMPHASIS ON CHICKEN MELANOMA DIFFERENTIATION-ASSOCIATED GENE 5 (MDA5)","authors":"Chih-Chun Lee, Chun-Yu Tung, C. Wu, T. Lin","doi":"10.1142/S1682648519300016","DOIUrl":"https://doi.org/10.1142/S1682648519300016","url":null,"abstract":"Avian species have immune system to fight invading pathogens. The immune system comprises innate and adaptive immunity. Innate immunity relies on pattern recognition receptors to sense particular molecules present in pathogens, i.e. pathogen-associated molecular patterns (PAMPs), or danger signals in the environment, i.e. danger-associated molecular patterns (DAMPs). Cytoplasmic retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) and nucleotide-binding oligomerization domain-like receptors (NLRs) are the sensors recognizing cytoplasmic PAMP and/or DAMP. Among common avian species, chickens do not have RIG-I whereas ducks and finches do. Therefore, the other RLR member, melanoma differentiation-associated gene 5 (MDA5), is believed to play an important role to recognize intracellular pathogens in chickens. Chicken MDA5 has been identified and its function determined. Chicken MDA5 maintains the same domain architecture compared with MDA5 analogs in other animal species. The expression of chicken MDA5 was upregulated when a synthetic double-stranded RNA (dsRNA), polyriboinosinic:polyribocytidylic acids (poly(I:C)), was transfected into chicken cells, whereas that did not change when cells were incubated with poly(I:C). The enhanced expression of chicken MDA5 in chicken cells upregulated the expression of chicken interferon-[Formula: see text] (IFN-[Formula: see text]). The infection of dsRNA infectious bursal disease virus (IBDV) in non-immune cells triggered the activation of chicken MDA5 signaling pathway, leading to the production of IFN-[Formula: see text] and subsequent response of IFN-stimulated genes. Furthermore, in immune cells like macrophages, chicken MDA5 participated in sensing the infection of IBDV by activating downstream antiviral genes and molecules and modulating adaptive immunity.On the contrary, one of cytoplasmic NLR member, NLR family pyrin domain containing 3 (NLRP3), was cloned and functionally characterized in chicken cells. Chicken NLRP3 conserved the same domain architecture compared with NLRP3 analogs in other animal species. Chicken NLRP3 was highly expressed in kidney, bursa of Fabricius and spleen. The production of mature chicken interleukin 1 [Formula: see text] (IL-1[Formula: see text] in chicken macrophages was stimulated by lipopolysaccharide (LPS) treatment followed by short ATP exposure.In summary, chicken MDA5 was a cytoplasmic dsRNA sensor that mediated the production of type I IFN upon ligand engagement, whereas NLRP3 sensed danger signals, such as ATP, in the cytoplasm and cleaved pro-IL-1[Formula: see text] to produce mature IL-1[Formula: see text]. Chicken MDA5 was not only involved in the activation of innate immune responses in non-immune and immune cells, but it also participated in modulating adaptive immunity in immune cells. Chicken NLRP3 participated in the production of mature chicken IL-1[Formula: see text] upon ligand engagement.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90270851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shu-Fang Yang, Chung-Chao Chen, P. Chang, Jian-Xi Yu, W. Liang, Chengjin Liu, Heng-Leng Yang, S. Liang
{"title":"CASE REPORT: ODONTOGENIC CUTANEOUS FISTULA IN THE SUBMANDIBULAR CERVICAL SKIN IN TWO DOGS","authors":"Shu-Fang Yang, Chung-Chao Chen, P. Chang, Jian-Xi Yu, W. Liang, Chengjin Liu, Heng-Leng Yang, S. Liang","doi":"10.1142/S1682648519720041","DOIUrl":"https://doi.org/10.1142/S1682648519720041","url":null,"abstract":"Odontogenic cutaneous fistula is commonly misdiagnosed by practitioners, leading to incorrect and unnecessary treatment. Here, we present two cases. Case 1 involved an eight-year-old intact male Maltese with a submandibular cutaneous abscess following 2 weeks of antibiotic treatment who was later diagnosed, using dental radiography, with odontogenic cutaneous fistula. Case 2 involved an eight-year-old neutered female Maltese with a submandibular cutaneous nodule and general skin problems following 6 months of oral antibiotics and a history of surgical intervention who was finally diagnosed, using dental radiography and computed tomography, with an odontogenic cutaneous fistula that resolved completely following tooth extraction. These two cases highlight the importance of odontogenic infection in the differential diagnosis of typical lesions in the head and neck.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76542692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"YEAR-LONG ULTRASONOGRAPHIC OBSERVATION OF FOLLICULAR FEATURES IN CAPTIVE YELLOW-MARGINED BOX TURTLES (CUORA FLAVOMARGINATA) FROM TAIWAN","authors":"Sheng-Hai Wu, Hao-Ping Tang, Hsien-Chi Wang, Linus Chang, Wei-Ming Lee, Kuan-Sheng Chen","doi":"10.1142/S1682648519500045","DOIUrl":"https://doi.org/10.1142/S1682648519500045","url":null,"abstract":"Ultrasonography is widely used to examine reproductive systems in chelonians. Previous studies have described how follicular size is associated with the reproductive stages of yellow-margined box turtles (YMBTs), but our understanding of follicular morphology remains limited. This study describes the different ultrasonographic appearances of preovulatory follicles of these turtles in Taiwan. Randomly selected 28 adult captive female turtles were examined under ultrasonography, weekly from November 2012 to November 2013. The females were divided into ovulatory and non-ovulatory groups with equal number coincidentally based on the whole-year ovulation records. The ovulatory group had significantly larger follicles than the non-ovulatory group (average size of largest follicle, [Formula: see text][Formula: see text]mm versus [Formula: see text][Formula: see text]mm; [Formula: see text]). Across both groups, preovulatory follicles exhibited five different morphologies: previtellogenic, homogeneous, heterogeneous, atretic, and multilayered. The multilayered follicles were only observed in the ovulatory group during the late preovulatory (February) and early ovulatory periods (March), resulting in a significant correlation with ovulatory stage ([Formula: see text]). During the preovulatory period (from December to February), homogeneous follicles were the predominant follicles in both groups. During the ovulation period, heterogeneous follicles were the main follicles, but only in the ovulatory group. Finally, atretic follicles were predominant in the non-ovulatory group during the early latent period (August). This study provides detailed ultrasonographic data clarifying the correlation between turtle follicular morphology and reproductive cycle. A better understanding of how such morphology is associated with reproductive stages should improve breeding programs in zoos and thus facilitate conservation efforts.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85300400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GENOME-WIDE METHYLOME ANALYSIS USING METHYLCAP-SEQ UNCOVERS NOVEL METHYLATED MARKERS FOR CANINE LYMPHOMA CELL LINE CLBL-1","authors":"Yang-Chi-Dung Lin, Hsi-Yuan Huang, Men-Yee Chiew, Chia-Hsin Hsu, S. Shrestha, Meng-Chu Wu, Chen-Si Lin, Hsien-Da Huang","doi":"10.1142/S1682648519500033","DOIUrl":"https://doi.org/10.1142/S1682648519500033","url":null,"abstract":"The most common type of tumor occurring in canine is the lymphomas. Epigenetic factors like DNA methylation is one of the important factors for causing cancer in canine. Cytology-based screening methods, such as fine needle aspiration (FNA) or immunocytochemistry (ICC), for canine lymphomas lack sensitivity. Despite an improvement in the detection of canine lymphomas by the DNA methylation analysis of lymph scrapings, only few methylation markers have been described. Even though technique like Digital Restriction Enzyme Analysis of Methylation (DREAM) can analyze the methylation profile of canine lymphoma cell line CLBL-1 at very low experimental cost without specific antibodies, the sensitivity and accuracy remain low in comparison to MethylCap-seq. In this study, a methylated DNA fragment of CLBL-1 was successfully captured by a specific protein for MethylCap-seq, and the methylation map of CLBL-1 was reconstructed by high throughput sequencing. For the first time, the genomic methylation region of canine CLBL-1 was analyzed. Using CLBL-1, we aimed to identify novel methylated markers for monitoring canine lymphomas. With the aid of methylation maps, several researchers have attempted to explore the relationship between DNA methylation and specific cancers. Additionally, the discovery of important biomarkers can predict or observe the course of canine cancer. Finally, the proposed advanced methylation information is likely to be suitable as a useful reference for clinical application of canine lymphoma research.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90507912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shu-Fang Yang, Heng-Leng Yang, S. Liang, Peng-Chen Wang
{"title":"CASE REPORT: SUBCONJUNCTIVAL INJECTION WITH AUTOLOGOUS PLATELET-RICH PLASMA FOR REFRACTORY CORNEAL ULCERS IN A DOG","authors":"Shu-Fang Yang, Heng-Leng Yang, S. Liang, Peng-Chen Wang","doi":"10.1142/S1682648518720034","DOIUrl":"https://doi.org/10.1142/S1682648518720034","url":null,"abstract":"A 12-year-old, intact, male Golden Retriever dog presented with a 1-month history of corneal ulcer, redness, and occasional squint in the left eye (oculus sinister [OS]). A thorough ophthalmological examination revealed corneal edema, diffuse corneal vascularization, and excessive granulation tissue growth. The tentative diagnosis was chronic superficial keratitis or pannus. After 3 weeks of treatment for OS, blepharospasm and superficial corneal ulceration with a lip of nonadherent epithelium of the right eye (oculus dexter [OD]) developed. No improvement was observed after autologous serum eye drop treatment combined with simple debridement and grid keratotomy on both eyes (oculi uterque [OU]) for 3 months; thereafter, blindness developed. The diagnosis was refractory corneal ulcers. The application of autologous platelet-rich plasma (PRP) was instilled via subconjunctival injection OU every 2 weeks for 2 months. The OU corneas started to re-epithelialize and heal, and the granulation tissues significantly decreased while the vision improved. Therefore, PRP was continued to be administered every 1–2 months for 6 months. After more than 1 year, the OU corneas have healed without recurrent ulceration and with only mild haze. In conclusion, autologous PRP subconjunctival injection is a potentially beneficial addition to the medical management of refractory corneal ulcers in dogs.","PeriodicalId":22157,"journal":{"name":"Taiwan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88734488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}