Surgical Neurology最新文献

Cardiac ventricular myosin and slow skeletal myosin exhibit dissimilar chemomechanical properties despite bearing the same myosin heavy chain isoform. 尽管心室肌球蛋白和慢骨骼肌球蛋白具有相同的肌球蛋白重链同工酶，但它们却表现出不同的化学机械特性。

Surgical Neurology Pub Date : 2022-07-01 Epub Date: 2022-05-24 DOI: 10.1016/j.jbc.2022.102070

Tianbang Wang, Emrulla Spahiu, Jennifer Osten, Florentine Behrens, Fabius Grünhagen, Tim Scholz, Theresia Kraft, Arnab Nayak, Mamta Amrute-Nayak

{"title":"Cardiac ventricular myosin and slow skeletal myosin exhibit dissimilar chemomechanical properties despite bearing the same myosin heavy chain isoform.","authors":"Tianbang Wang, Emrulla Spahiu, Jennifer Osten, Florentine Behrens, Fabius Grünhagen, Tim Scholz, Theresia Kraft, Arnab Nayak, Mamta Amrute-Nayak","doi":"10.1016/j.jbc.2022.102070","DOIUrl":"10.1016/j.jbc.2022.102070","url":null,"abstract":"The myosin II motors are ATP-powered force-generating machines driving cardiac and muscle contraction. Myosin II heavy chain isoform-beta (β-MyHC) is primarily expressed in the ventricular myocardium and in slow-twitch muscle fibers, such as M. soleus. M. soleus-derived myosin II (SolM-II) is often used as an alternative to the ventricular β-cardiac myosin (βM-II); however, the direct assessment of biochemical and mechanical features of the native myosins is limited. By employing optical trapping, we examined the mechanochemical properties of native myosins isolated from the rabbit heart ventricle and soleus muscles at the single-molecule level. We found purified motors from the two tissue sources, despite expressing the same MyHC isoform, displayed distinct motile and ATPase kinetic properties. We demonstrate βM-II was approximately threefold faster in the actin filament-gliding assay than SolM-II. The maximum actomyosin (AM) detachment rate derived in single-molecule assays was also approximately threefold higher in βM-II, while the power stroke size and stiffness of the \"AM rigor\" crossbridge for both myosins were comparable. Our analysis revealed a higher AM detachment rate for βM-II, corresponding to the enhanced ADP release rates from the crossbridge, likely responsible for the observed differences in the motility driven by these myosins. Finally, we observed a distinct myosin light chain 1 isoform (MLC1sa) that associates with SolM-II, which might contribute to the observed kinetics differences between βM-II and SolM-II. These results have important implications for the choice of tissue sources and justify prerequisites for the correct myosin heavy and light chains to study cardiomyopathies.","PeriodicalId":22153,"journal":{"name":"Surgical Neurology","volume":"22 1","pages":"102070"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75522220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moyamoya disease.

Surgical Neurology Pub Date : 2020-02-10 DOI: 10.32388/uejoi3

J. Dillion, H. Stokes, A. Meirowsky

引用次数: 0

PNIPAAM modified mesoporous hydroxyapatite for sustained osteogenic drug release and promoting cell attachment. PNIPAAM 改性介孔羟基磷灰石用于持续释放成骨药物和促进细胞附着。

IF 7.9

Surgical Neurology Pub Date : 2016-05-01 Epub Date: 2016-01-08 DOI: 10.1016/j.msec.2016.01.012

Tao Wu, Lei Tan, Ning Cheng, Qi Yan, Yu-Feng Zhang, Chuan-Jun Liu, Bin Shi

{"title":"PNIPAAM modified mesoporous hydroxyapatite for sustained osteogenic drug release and promoting cell attachment.","authors":"Tao Wu, Lei Tan, Ning Cheng, Qi Yan, Yu-Feng Zhang, Chuan-Jun Liu, Bin Shi","doi":"10.1016/j.msec.2016.01.012","DOIUrl":"10.1016/j.msec.2016.01.012","url":null,"abstract":"This work presented a sustained release system of simvastatin (SIM) based on the mesoporous hydroxyapatite (MHA) capped with poly(N-isopropylacrylamide) (PNIPAAM). The MHA was prepared by using cetyltrimethylammonium bromide (CTAB) as a template and the modified PNIPAAM layer on the surface of MHA was fabricated through surface-initiated atom transfer radical polymerization (SI-ATRP). The SIM loaded MHA-PNIPAAM showed a sustained release of SIM at 37 °C over 16 days. The bone marrow mesenchymal stem cell (BMSC) proliferation was assessed by cell counting kit-8 (CCK-8) assay, and the osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity and Alizarin Red staining. The release profile showed that the release of SIM from MHA-SIM-PNIPAAM lasted 16 days and the cumulative amount of released SIM was almost seven-fold than MHA-SIM. Besides, SIM loaded MHA-PNIPAAM exhibited better performance on cell proliferation, ALP activity, and calcium deposition than pure MHA due to the sustained release of SIM. The quantity of ALP in MHA-SIM-PNIPAAM group was more than two fold than pure MHA group at 7 days. Compared to pure MHA, better BMSC attachment on PNIPAAM modified MHA was observed using fluorescent microscopy, indicating the better biocompatibility of MHA-PNIPAAM.","PeriodicalId":22153,"journal":{"name":"Surgical Neurology","volume":"42 1","pages":"888-96"},"PeriodicalIF":7.9,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75546487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomedical research 生物医学研究

Surgical Neurology Pub Date : 2012-01-01 DOI: 10.1016/0090-3019(85)90077-1

Paul C. Bucy M.D. (Editor)

引用次数: 40

Re: The neuroprotective effect of dexmedetomidine in the hippocampus of rabbits after subarachnoid hemorrhage (Cosar et al. Surg Neurol 2009;71:54-59) 右美托咪定对兔蛛网膜下腔出血后海马的神经保护作用(Cosar等)。中华外科杂志(英文版);2009;31 (1):59 - 64

Surgical Neurology Pub Date : 2009-12-01 DOI: 10.1016/j.surneu.2009.02.002

Paul S. Slosberg MD

引用次数: 2

Addendum to “In this issue…” “在本期中……”的附录

Surgical Neurology Pub Date : 2009-12-01 DOI: 10.1016/j.wneu.2009.11.001

James I. Ausman MD, PhD ((Editor))