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Cloning and Expression of the Cellulase Gene from the King Oyster Mushroom, Pleurotus eryngii. 王氏平菇纤维素酶基因的克隆与表达。
Silpakorn University Science and Technology Journal Pub Date : 2016-07-22 DOI: 10.14456/SUSTJ.2016.17
U. Romruen, E. Bangyeekhun
{"title":"Cloning and Expression of the Cellulase Gene from the King Oyster Mushroom, Pleurotus eryngii.","authors":"U. Romruen, E. Bangyeekhun","doi":"10.14456/SUSTJ.2016.17","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.17","url":null,"abstract":"A gene encoding for cellobiohydrolase (PEcbh) from P. eryngii was cloned by using RT-PCR 3’ and 5’ RACE techniques. The result showed that the PEcbh was 1377 bp nucleotide sequence encoded for 459-deduced amino acid. Analysis of predicted protein revealed that PEcbh consisted of a glycosyl hydrolase family 7 domain but lacked of cellulose binding domain, a calculated molecular weight of 49.3 kDa and a pI of 5.3. The PEcbh was cloned into pET28a (+) to obtain a recombinant pET/PEcbh and expressed in E. coli BL21 (DE3). The optimal conditions of PEcbh expression were 0.2 mM IPTG, 1 h induction time at 180C and 4 h post-induction time. The CMCase activity could be detected, but at a low activity. This is probably due to a lack of the cellulose binding domain in PEcbh. Expression of PEcbh in E. coli BL21 (DE3) and Rosetta (DE3) were compared and the results indicated that CMCase activity in Rosetta (DE3) was higher than in BL21 about 2 times.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"70 1","pages":"22-30"},"PeriodicalIF":0.0,"publicationDate":"2016-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87359568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of Meropenem Use Among Septic Patients at Hua Hin Hospital, Thailand 泰国华欣医院脓毒症患者使用美罗培南的评价
Silpakorn University Science and Technology Journal Pub Date : 2016-04-28 DOI: 10.14456/SUSTJ.2016.2
Wandee Sumret, Kamonphop Saowakul, Thirawan Haephruek, Penprapa Pengsombat, Witchuda Kwanmuang, W. Santimaleeworagun
{"title":"Evaluation of Meropenem Use Among Septic Patients at Hua Hin Hospital, Thailand","authors":"Wandee Sumret, Kamonphop Saowakul, Thirawan Haephruek, Penprapa Pengsombat, Witchuda Kwanmuang, W. Santimaleeworagun","doi":"10.14456/SUSTJ.2016.2","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.2","url":null,"abstract":"Antimicrobial-resistant bacteria are an important medical concern. This problem might be resulted from an inappropriate use of antibiotics. The review of antibiotic drug use could help understanding the cause of drug resistance and eventually resolve the problem. Our retrospective cohort study aimed to review meropenem use at Hua-hin hospital, Prachuap Khiri Khan, including therapeutic indications, dosage regimens and duration of sepsis treatment. Data were gathered from patients admitted in the hospital during January to December 2013. The sources of data included patient medical records, patient database from computer program and in-house form of meropenem evaluation. A total of thirty-six cases were evaluated, 17 cases (47.2%) were male, and the average age was 58.3 (±19.9) years. There were 22 cases (61.1%) and 14 cases (38.9%) receiving meropenem as empirical and definitive therapy, respectively. According to meropenem use evaluation, there were 29 (80.6%), 33 (91.7%) and 24 (85.7%) cases that met the criteria for indication, dosage regimen, and treatment duration, respectively. Only 17 cases (60.7%; n=28) met all three criteria for appropriate meropenem use. Thus, with our finding, the role of healthcare professionals in reducing the inappropriate use of antibiotics for the entire course of treatment should be encouraged.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"24 1","pages":"21-26"},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81185736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Nutritive Value, Digestibility and Gas Production of Fermented Sugar Palm Peel with Pineapple Peel 菠萝皮发酵糖棕榈皮的营养价值、消化率及产气量
Silpakorn University Science and Technology Journal Pub Date : 2016-04-28 DOI: 10.14456/SUSTJ.2016.4
P. Saenphoom
{"title":"Nutritive Value, Digestibility and Gas Production of Fermented Sugar Palm Peel with Pineapple Peel","authors":"P. Saenphoom","doi":"10.14456/SUSTJ.2016.4","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.4","url":null,"abstract":"An experiment was conducted to examine the nutritive value, digestibility and gas production of fermented sugar palm peel with pineapple peel. The treatment was conducted as a 3×2 factorial in a completely randomized design (CRD). The experiment consisted of six treatment combinations; sugar palm peel to pineapple peel ratios (2:1, 1:1 and 1:2 by weight) and fermentation times (day-14 and day-21). All treatment combinations were fermented under anaerobic condition in airtight plastic pouches. The result showed that ether extract content of fermented sugar palm peel with pineapple peel in 2:1 ratio at day-14 was significantly higher than other treatment combinations (P 0.05) thereafter (24 hr, 48 hr and 72 hr). Fermented sugar palm peel with pineapple peel in 2:1 ratio at day-21 recorded the highest gas volume (45.16 ml). Results of this study suggested that the fermented sugar palm peel with pineapple peel in 1:2 ratio at day-14 produced a lower fiber content and higher digestibility silage, making it a suitable silage for ruminant animal since high fiber content had adverse effect on the nutrients digestibility.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"103 1","pages":"32-37"},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85861081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Preparation, Characterization and Antibacterial Study of Novel 2-(5-(4-Chlorophenyl)-4, 5-dihydro-1, 2-oxazol-3-yl) Compounds 新型2-(5-(4-氯苯基)- 4,5 -二氢- 1,2 -恶唑-3-基)化合物的制备、表征及抗菌研究
Silpakorn University Science and Technology Journal Pub Date : 2016-04-28 DOI: 10.14456/SUSTJ.2016.3
K. Mehta
{"title":"Preparation, Characterization and Antibacterial Study of Novel 2-(5-(4-Chlorophenyl)-4, 5-dihydro-1, 2-oxazol-3-yl) Compounds","authors":"K. Mehta","doi":"10.14456/SUSTJ.2016.3","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.3","url":null,"abstract":"This research paper describes a study about synthesis, structure determination and antibacterial activity evaluation of some novel heterocyclic compounds having 2-[5-(4-chlorophenyl)-4,5-dihydro-1,2-oxazol-3-yl] fragment. To prepare this kind of compounds, 3-(4-chlorophenyl)-1-(2-hydroxyphenyl)prop-2-en-1-one was prepared. Its coupling reaction was carried out with diazonium salt of 1o aromatic amine. The coupled product was separated and reacted with hydroxylamine hydrochloride. The final product containing 2-[5-(4-chlorophenyl)-4,5-dihydro-1,2-oxazol-3-yl] fragment was characterized with the help of melting point determination and elemental analysis of carbon, hydrogen, nitrogen and chlorine elements. Further characterization was performed with the help of IR, 1H NMR and Mass spectroscopic methods. The characterization study supports the projected structures of heterocyclic derivatives. Antibacterial assay in vitro for these compounds was carried out for Pseudomonas aeruginosa, Bacillus subtilis, Erwinia carotovora and Escherichia coli. It showed good antibacterial activity.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"12 1","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79540289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nurse’s Shift Balancing in Nurse Scheduling Problem 护士排班问题中的护士轮班平衡
Silpakorn University Science and Technology Journal Pub Date : 2016-04-28 DOI: 10.14456/SUSTJ.2016.6
Kanjana Thongsanit, Kanokwan Kantangkul, Thonphakkhaon Nithimethirot
{"title":"Nurse’s Shift Balancing in Nurse Scheduling Problem","authors":"Kanjana Thongsanit, Kanokwan Kantangkul, Thonphakkhaon Nithimethirot","doi":"10.14456/SUSTJ.2016.6","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.6","url":null,"abstract":"In this study, a load balanced nurse-scheduling model is developed. A case problem of a local hospital in Ratchaburi province is used to test the model in order to improve the existing manual method. There are three working shifts, morning shift, afternoon shift and night shift in the hospital. The load balance is one of factors that affects the nurse’s preferences. The objective of the study is to balance the load of each shift for all workers. The min-max objective is applied to minimize the maximum deviation of the average load of each shift. Goal constraints is applied to determine the plus and minus deviation from the average load of each shift. The proposed model is solved by the Premium Solver on Microsoft Excel. It was found that the balanced load is improved.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"57 1","pages":"43-48"},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76384408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Influence of Exogenous Spermidine on to Peel Color Change and Browning Symptom of Musa (AAA group) cv. Kluai Hom Thong at Low Temperature Storage 外源亚精胺对芭蕉果皮颜色变化及褐变症状的影响(AAA组)葵红通的低温贮藏
Silpakorn University Science and Technology Journal Pub Date : 2016-04-28 DOI: 10.14456/SUSTJ.2016.5
Phrutiya Nilprapruck, P. Meetum, C. Chanthasa
{"title":"Influence of Exogenous Spermidine on to Peel Color Change and Browning Symptom of Musa (AAA group) cv. Kluai Hom Thong at Low Temperature Storage","authors":"Phrutiya Nilprapruck, P. Meetum, C. Chanthasa","doi":"10.14456/SUSTJ.2016.5","DOIUrl":"https://doi.org/10.14456/SUSTJ.2016.5","url":null,"abstract":"Musa (AAA Group) cv. Kluai Hom Thong is popular and widely consumed fruits in Thailand and overseas. This experiment showed an influence of spermidine (SPD) on change of peel color and browning in banana. Ripe bananas were soaked with 0, 0.2, 0.5 and 2.0 mM and then stored at 10 oC, 85±5 %RH. Bananas, treated with 0.5 and 2.0 mM SPD, showed alteration of biochemical changes such as color, ripping and enzymatic browning reaction. Biochemical changes included chlorophyll, carotenoid, phenolic compounds, as well as polyphenol oxidase and peroxidase activities which increased during storage. Bananas, treated with SPD at 0.5 and 2.0 mM, exhibited a reduction in an accumulation of phenolic compounds, polyphenol oxidase and peroxidase activities, comparing with the non-treated SPD samples.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"1 1","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82241269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Demographic Factors and Health Care Behavior of Hypertension Disease in Thailand 泰国高血压病的人口因素与保健行为
Silpakorn University Science and Technology Journal Pub Date : 2015-02-04 DOI: 10.14456/SUSTJ.2015.5
Sukanya Chongthawonsatid
{"title":"Demographic Factors and Health Care Behavior of Hypertension Disease in Thailand","authors":"Sukanya Chongthawonsatid","doi":"10.14456/SUSTJ.2015.5","DOIUrl":"https://doi.org/10.14456/SUSTJ.2015.5","url":null,"abstract":"The purpose of this study is to examine demographic factors and health care behaviors associated with hypertension disease. A retrospective study was performed to analyze data from the Health and Welfare Survey of the National Statistical Office, Thailand (2007). The sample population consisted of 31,088 individuals. Analysis of the data found that females were 1.48 times more likely to suffer from hypertension disease than males. Aging individuals, 60 years old and older, were more likely to have hypertension disease. People who live in the Northeastern and the Southern regions of Thailand were less likely to have hypertension disease in comparison with individuals who live in Bangkok metropolitan areas. Males exercise more often than females, however, males are not able to reduce or discontinue smoking at a higher level than females. Males’ reduction or discontinuation of alcohol consumption is at a lower level than females. Reduction or discontinuation of alcohol consumption of age group of 25-59 years old also is at a lower level than those 59 years old and older. People who live in the Central region have the lowest level of routine exercise, diet control, and weight reduction when compared to those in Bangkok, Northern, Southern, or Northeastern regions. People who live in the Northern region have the lowest reduction or discontinuation of smoking and consumption of alcohol in comparison with those in Bangkok, Central, Southern, and the Northeastern regions. Further research should consider the contextual differences in each region regarding health education and health promotion, especially exercise for people who live in the Central region, and the reduction or discontinuation of alcohol and smoking for those in the Northern region.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"3 1","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"2015-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82836116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Regeneration of Adventitious Shoots from Callus and Leaf Explants in Jatropha curcas L. ‘Phetchaburi’ 麻疯树愈伤组织和叶片外植体不定芽再生的研究
Silpakorn University Science and Technology Journal Pub Date : 2015-02-04 DOI: 10.14456/SUSTJ.2015.3
P. N. Chiangmai, Y. Pootaeng-on, P. Meetum, K. Kamkajon, Waraporn Yuiam, Narumon Rungphan, Panwadee Ninkaew
{"title":"Regeneration of Adventitious Shoots from Callus and Leaf Explants in Jatropha curcas L. ‘Phetchaburi’","authors":"P. N. Chiangmai, Y. Pootaeng-on, P. Meetum, K. Kamkajon, Waraporn Yuiam, Narumon Rungphan, Panwadee Ninkaew","doi":"10.14456/SUSTJ.2015.3","DOIUrl":"https://doi.org/10.14456/SUSTJ.2015.3","url":null,"abstract":"The effect of cytokinins and auxins was evaluated in inducing a physic nut (Jatropha curcas L.) callus while the effect of the combinations of cytokinin and either indole-3-butyric acid (IBA) or some growth additives was determined in regenerating adventitious shoot from a leaf segment. The results showed MS medium containing only 0.1 or 1.0 mg/l thidiazuron (TDZ) was the most effective medium for inducing morphogenic callus. MS medium supplemented with 2.0 mg/l 6-benzylaminopurine (BA) and 0.5 mg/l indole-3-acetic acid (IAA) gave the highest shoot regeneration percentage and shoot height from the callus. On the other hand, the MS medium supplemented with 0.5 mg/l BA and 1.0 mg/l IBA was the most effective medium for adventitious shoot regeneration and shoot height from the leaf segments.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"12 1","pages":"28-39"},"PeriodicalIF":0.0,"publicationDate":"2015-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80458036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Thai License Plate Recognition System From a Video Stream 泰国车牌识别系统从视频流
Silpakorn University Science and Technology Journal Pub Date : 2014-06-04 DOI: 10.14456/SUSTJ.2014.9
C. Pornpanomchai, Nuchakarn Anawatmongkon
{"title":"Thai License Plate Recognition System From a Video Stream","authors":"C. Pornpanomchai, Nuchakarn Anawatmongkon","doi":"10.14456/SUSTJ.2014.9","DOIUrl":"https://doi.org/10.14456/SUSTJ.2014.9","url":null,"abstract":"The objective of this project was to develop a computer system which is automatically capable of localization, segmentation and recognition of a Thai license plate from a video file. The system was called “Thai license plate recognition system” or “TLPRS”. The TLPRS consisted of 5 modules, which were 1) video image acquisition, 2) license plate localization, 3) character segmentation, 4) character recognition, and 5) result presentation. The experiment was conducted on video files and localized car license platesfrom video frames in every second. After that the system segmented each character and recognized it. The experiment was conducted on video files with total length of 2.5 hours, which consisted of 497 car images. Based on the experimental results, the precision rate of license plate localization was 91.95 percent, while the character segmentation rate was 64.19 percent and the character recognition rate was 93.26 percent.","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"81 1","pages":"40-50"},"PeriodicalIF":0.0,"publicationDate":"2014-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76945750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Technology Transfer and Technological Capability: A Case Study in Manufacturing Process in Thai Pharmaceutical Industry 技术转移与技术能力:以泰国制药业生产过程为例
Silpakorn University Science and Technology Journal Pub Date : 2014-05-27 DOI: 10.14456/SUSTJ.2014.10
Onoomar Toyama, S. Kongmuang, M. Toyama
{"title":"Technology Transfer and Technological Capability: A Case Study in Manufacturing Process in Thai Pharmaceutical Industry","authors":"Onoomar Toyama, S. Kongmuang, M. Toyama","doi":"10.14456/SUSTJ.2014.10","DOIUrl":"https://doi.org/10.14456/SUSTJ.2014.10","url":null,"abstract":"800x600 Forms, channels, difficulties and obstacles of technology transfer in manufacturing process and pharmaceutical production, including technological capability were obtained from experts and modern Thai pharmaceutical companies through a survey research. Results showed thatThai pharmaceutical industry received a transfer of technology mostly in the form of printed documents, articles on the internet, and attending trade exhibitions and academic conferences. The first three channels for technology transfer were training received from local academic institutions, self-study from product manuals, and instruction and guidance from technology donors. Difficulties and obstacles in technology transfer were technology performing by the recipient, the hospitality of technology donor, the handover of technology, and the capability of the recipient. In building up technological capability, Thai pharmaceutical manufacturers depended on outside personnel in the acquisition for sources of technology, installation and adaptation of technology with local condition, and product innovation. Findings from this study indicated the status and the efficiency of technology transfer as well as the technological capability of Thai pharmaceutical companies. Results will support the strategy in technological development and build up the competitiveness of Thai pharmaceutical industry, especially among the ASEAN countries. Normal 0 false false false EN-US X-NONE TH /* Style Definitions */ \u0000 table.MsoNormalTable \u0000 {mso-style-name:\"Table Normal\"; \u0000 mso-tstyle-rowband-size:0; \u0000 mso-tstyle-colband-size:0; \u0000 mso-style-noshow:yes; \u0000 mso-style-priority:99; \u0000 mso-style-parent:\"\"; \u0000 mso-padding-alt:0cm 5.4pt 0cm 5.4pt; \u0000 mso-para-margin:0cm; \u0000 mso-para-margin-bottom:.0001pt; \u0000 mso-pagination:widow-orphan; \u0000 font-size:10.0pt; \u0000 font-family:\"Times New Roman\",\"serif\"; \u0000 mso-bidi-font-family:\"Angsana New\";}","PeriodicalId":22107,"journal":{"name":"Silpakorn University Science and Technology Journal","volume":"193 1","pages":"51-61"},"PeriodicalIF":0.0,"publicationDate":"2014-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79703476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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