Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology最新文献_第4页

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii 莫里比多毛藻烃类降解菌的分离与鉴定

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-12-17 DOI: 10.15578/squalen.567

Fazilah Arifin, R. Razali, V. Sevakumaran

{"title":"Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii","authors":"Fazilah Arifin, R. Razali, V. Sevakumaran","doi":"10.15578/squalen.567","DOIUrl":"https://doi.org/10.15578/squalen.567","url":null,"abstract":"Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87649622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Caulerpa racemosa (Caulerpales, Chlorophyta) from Indonesia based on the Plastid tufA Gene 基于质体tufA基因的印尼总状茎叶(Caulerpa racemosa)分子特征分析

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-12-13 DOI: 10.15578/squalen.588

M. Darmawan, N. Zamani, H. Irianto, H. Madduppa

{"title":"Molecular characterization of Caulerpa racemosa (Caulerpales, Chlorophyta) from Indonesia based on the Plastid tufA Gene","authors":"M. Darmawan, N. Zamani, H. Irianto, H. Madduppa","doi":"10.15578/squalen.588","DOIUrl":"https://doi.org/10.15578/squalen.588","url":null,"abstract":"The green seaweed Caulerpa racemosa is a seaweed of high prospect that is being given emphasis by the Indonesian government. However, C. racemosa in Indonesia may include multiple species level-entity exhibiting morphological overlap and require molecular analysis for species identification. Molecular documentation of species richness of indigenous populations of C. racemosa is essential to underpin cultivar development and conservation of the species to avoid overharvesting. The present study aimed to determine the genetic diversity of C. racemosa and document the haplotype network of the specimens from four different locations (Bintan Island, Jepara, Takalar and Osi Island) using the chloroplast tufA gene. Twenty individuals from four areas were collected and amplified with the chloroplast-encoded gene tufA for species identification against publicly available data. The identification of C. racemosa based on the tufA gene showed that the species found in four locations were C. cylindracea (previously C. racemosa var. cylindracea), C. macra (previously C. racemosa var. macra), C. racemosa, and C. oligophylla (previously C. racemosa var. lamourouxii). This study records the existence of C. cylindracea in Takalar and Jepara, Indonesia for the first time. The most diverse C. racemosa species was in Osi Island, where the exploitation of this seaweed is very low. In contrast, the lowest number of C. racemosa varieties were found in Takalar, where exploitation is very high. There were only minor light variations of Caulerpa species in the tufA gene in four different sites with only four haplotypes found, and each haplotype corresponded to another species. ","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83784513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Characteristics of Irradiated Hydrogel Formulated from Composite i/k-carrageenan Combined with Polyvinyl Alcohol i/k-卡拉胶与聚乙烯醇复合制备辐照水凝胶的特性

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-11-29 DOI: 10.15578/squalen.439

B. Utomo, D. Fransiska, La Ode Sumarlin, Ihya Sulthonuddin

{"title":"Characteristics of Irradiated Hydrogel Formulated from Composite i/k-carrageenan Combined with Polyvinyl Alcohol","authors":"B. Utomo, D. Fransiska, La Ode Sumarlin, Ihya Sulthonuddin","doi":"10.15578/squalen.439","DOIUrl":"https://doi.org/10.15578/squalen.439","url":null,"abstract":"Eucheuma seaweed from tropical waters habitat mainly contains carrageenan, a type of hydrocolloid potential for hydrogel production. This study aims to determine the characteristics of hydrogel formulated from composite i- and k-carrageenan combined with polyvinyl alcohol (PVA) which, could be used further for wound dressing application. The concentrations of composite i/k-carrageenan used were 1.0%, 1.5%, 2.0%, 2.5% and 3.0% w/w, and the PVA concentration was 12.5% w/w. Composite i/k-carrageenan in a proportion of 6:4 by weight. The hydrogel was irradiated using 60Co g-rays with the irradiation dose of 25 and 2 kGy.h-1. The concentration of composite i- and k-carrageenan influenced the gel fraction, water holding capacity, tensile strength, elongation, and surface morphology of the hydrogel. The optimum formula of irradiated composite hydrogel was obtained from 3.0% w/w of composite i/k-carrageenan. The formula had a gel fraction of 61.67%, water holding capacity of 1067.12%, tensile strength of 32.37 x 10-3 MPa, and elongation of 251.67%. Interestingly, the product with the optimum formula had a porous surface morphology and transparency, which are applicable for wound dressing purposes.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88479526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutrient Composition, Antioxidant and Antibacterial Activities of Ulva prolifera O. F. Müller 增生Ulva Ulva prolifera o.f. melller的营养成分及其抗氧化和抗菌活性

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-10-11 DOI: 10.15578/squalen.550

E. Arguelles, Arsenia B. Sapin

{"title":"Nutrient Composition, Antioxidant and Antibacterial Activities of Ulva prolifera O. F. Müller","authors":"E. Arguelles, Arsenia B. Sapin","doi":"10.15578/squalen.550","DOIUrl":"https://doi.org/10.15578/squalen.550","url":null,"abstract":"Seaweeds are marine organisms capable of producing diverse kinds of chemical compounds with promising pharmacological use. The study evaluated the proximate and elemental composition and the potential antioxidant (using CUPRAC, ABTS+, and DPPH assays) and antibacterial activities (using microtiter plate dilution assay) of Ulva prolifera O. F. Müller. The seaweed has a total phenolic content of 829 ± 2.00 mg GAE/g. Antioxidant efficiency of U. prolifera exerted high ability of reducing copper ions, potent ABTS+ and DPPH scavenging activities in a concentration-dependent manner with IC50 value of 24.7 mg GAE/mL, 43.52 mg GAE/mL, and 54.1 mg GAE/mL, respectively, more effective than ascorbic acid. In vitro antibacterial activity assay showed that U. prolifera exhibited inhibitory activity against bacterial pathogens such as Methicillin-resistant Staphylococcus aureus (MIC = 125 mg/mL), S. epidermidis (MIC = 125 mg/mL), and Pseudomonas fluorescens (MIC = 500 mg/mL). This investigation is the first documented report regarding antibacterial activity of U. prolifera against P. fluorescens. In addition, results showed elemental composition to be in decreasing order of Ca K Mg Na Mn Fe Zn Cu Pb Cd Cr. Proximate composition of U. prolifera showed high carbohydrate and protein content with a percentage composition of 36.20 ± 0.27% and 23.72 ± 0.31% (dry weight), respectively. This study is the first report in the Philippines that shows the potential of U. prolifera as an excellent candidate organism as source of chemical compounds with relevant application to the pharmacological industry.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86824192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Occurrence and Source Apportionment of Polycyclic Aromatic Hydrocarbons (PAHs) in Green Mussel (Perna viridis) from Cilincing Waters of Jakarta Bay, Indonesia 印尼雅加达湾沿岸绿贻贝(Perna viridis)中多环芳烃(PAHs)的赋存状态及来源分析

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-05-24 DOI: 10.15578/SQUALEN.484

D. Yogaswara, Tri Partono, A. Satya

{"title":"Occurrence and Source Apportionment of Polycyclic Aromatic Hydrocarbons (PAHs) in Green Mussel (Perna viridis) from Cilincing Waters of Jakarta Bay, Indonesia","authors":"D. Yogaswara, Tri Partono, A. Satya","doi":"10.15578/SQUALEN.484","DOIUrl":"https://doi.org/10.15578/SQUALEN.484","url":null,"abstract":"Green mussels (Perna viridis) as sedentary organisms have been adopted as bioindicators in the marine environment for many decades. They have been potentially affected by anthropogenic activity and organic contaminants such as polycyclic aromatic hydrocarbons (PAHs) in an aquatic environment. This study aims to determine concentration, distribution and potential sources of PAHs in green mussel biomass obtained from Cilincing waters, Jakarta Bay. Fresh green mussels were collected from aquaculture facilities at Cilincing waters of Jakarta Bay with various size categories of shell length and were extracted with solvent n-hexane-dichloromethane mixture using Soxhlet method. The concentrate of sample extract was purified using silica gel/sodium sulfate anhydrous with n-pentene-DCM effluent in fractionation. Finally, the purified sample was injected into the GC-MS instrument. The result showed that the highest concentration of total PAH (TPAH) was 126.47 ng.g-1 wet weight (ww), founded in the guts from the biggest group of green mussel (length of 6.00 to 7.99 cm), and 3-aromatic rings were predominantly detected in all group sizes of green mussel. The result of the LMW/ HMW and diagnostic binary ratio indicated that PAH contamination is caused by a mixed source of petrogenic and pyrogenic processes. It may be due to the presence of oil spilled and petroleum waste from shipyard and cargo activities and atmospheric deposition. Based on excess cancer risk (ECR) value and the concentration level of PAH carcinogenic according to National Agency of Drug and Food Control (BPOM) regulation, PAH level in green mussels are acceptable and minor negative impact due to typical human consumption. Nevertheless, it needs to be circumspect to exploiting green mussels as seafood resources for daily consumption due to the carcinogenic content.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90894861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Back Cover Squalen Bulletin Vol. 16 No. 1 Tahun 2021 封底角鲨公报第16卷第1期Tahun 2021

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-05-24 DOI: 10.15578/squalen.v16i1.563

Squalen Bulletin

引用次数: 0

Optimization of Chitosan Concentration on The Quality and Shelf Life of Frozen Rohu (Labeo rohita) Fillets 壳聚糖浓度对冷冻罗虎鱼片品质及保质期的影响

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-05-24 DOI: 10.15578/SQUALEN.504

Faria Afrin, M. Rasul, Murshida Khan, T. Akter, Chunhong Yuan, A. K. Shah

{"title":"Optimization of Chitosan Concentration on The Quality and Shelf Life of Frozen Rohu (Labeo rohita) Fillets","authors":"Faria Afrin, M. Rasul, Murshida Khan, T. Akter, Chunhong Yuan, A. K. Shah","doi":"10.15578/SQUALEN.504","DOIUrl":"https://doi.org/10.15578/SQUALEN.504","url":null,"abstract":"A natural product such as chitosan promotes health benefits and extends the shelf life of the processed products. Chitosan has been used as a food additive due to its antioxidant and antimicrobial activities. This study was conducted to optimize the chitosan concentration as a coating agent on the quality and shelf life of Labeo rohita fillets during frozen storage. Chitosan (CH) solutions were prepared with 0.5 %, 1 %, and 2 % (w/v) CH in 1 % (v/v) glacial acetic acid for coating application. The control (0% CH) and CH treated fish fillets were stored at -18 °C for 14 weeks and assessed for chemical (pH, thiobarbituric acid reactive substances (TBARS), total volatile basic nitrogen (TVB-N), and K-value), microbiological (aerobic plate count (APC)), and sensory properties. The pH, TBARS, and K-value of 1 % and 2 % CH treated fish fillets were acceptable up to 14th week of storage, while TVB-N value was permissible up to 12th week of storage. After 14th week of storage, the APC of control, 0.5 %, 1 %, and 2 % CH treated fillets were 7.18 log CFU/g, 5.02 log CFU/g, 4.13 log CFU/g, and 3.21 log CFU/g, respectively. The fish fillets treated with 1 % and 2 % CH had acceptable sensory attributes up to 12th week, while control fillets were unacceptable after the 6th week of storage. This study demonstrated that 1% CH could be used as a natural additive for keeping the quality and extending the shelf life of L. rohita fillets during frozen storage.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87898591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Front Cover Squalen Bulletin Vol. 16 No. 1 Tahun 2021 封面角鲨公报第16卷第1期Tahun 2021

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-05-24 DOI: 10.15578/squalen.v16i1.561

Bulletin Squalen

引用次数: 0

Bioactive Compounds, Cosmeceutical And Nutraceutical Applications of Green Seaweed Species (Chlorophyta) 绿色海藻的生物活性化合物、药妆和营养保健应用

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-05-24 DOI: 10.15578/SQUALEN.514

Prof. Muhammad Taher, Ph.D, Fatin Syazwani Ruslan, D. Susanti, N. Mohammad Noor, N. Aminudin

{"title":"Bioactive Compounds, Cosmeceutical And Nutraceutical Applications of Green Seaweed Species (Chlorophyta)","authors":"Prof. Muhammad Taher, Ph.D, Fatin Syazwani Ruslan, D. Susanti, N. Mohammad Noor, N. Aminudin","doi":"10.15578/SQUALEN.514","DOIUrl":"https://doi.org/10.15578/SQUALEN.514","url":null,"abstract":"Seaweeds are valuable marine plants that have garnered much attention from the public due to their high bioactive, nutrients and minerals content. Seaweeds have been used in multiple applications, including in cosmeceutical, nutraceutical and pharmaceutical industries. Nevertheless, this review will focus on the bioactive compounds of Chlorophyta and their potential application in nutraceutical and cosmeceutical industries. Chlorophyta are believed to possess a significant amount of nutrients and minerals, sufficient to meet the daily requirements of nutrients and minerals in the human body. Considering the nutritional aspect, deficiency in nutrients may lead to severe ailments, including heart disease, neurological disorder and cancer. The main compounds studied in this review are polysaccharides, proteins, amino acids, lipids, fatty acids, pigments, minerals, vitamins and secondary metabolites. Among all, polysaccharides are the most exploited compounds and used in many advanced applications in the nutraceutical and cosmeceutical industries. This review also offers insights into the beneficial biological properties of Chlorophyta, highlighting their potential in cosmeceutical and nutraceutical applications. Further research is required to highlight the Chlorophyta sp. aquaculture, its extraction method, and the most targeted bioactive compounds from the species. Therefore, the challenge is to increase public awareness of the promising application of this species in the nutraceutical and cosmeceutical industries.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84627674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Heat Resistance of Candidate Probiotic Enterococcus faecalis R22B in Different Matrices 候选益生菌粪肠球菌R22B在不同基质中的耐热性

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pub Date : 2021-04-16 DOI: 10.15578/SQUALEN.497

S. Lestari, R. Rinto, I. Wahyuni, S. Ridhowati, W. Wulandari

{"title":"Heat Resistance of Candidate Probiotic Enterococcus faecalis R22B in Different Matrices","authors":"S. Lestari, R. Rinto, I. Wahyuni, S. Ridhowati, W. Wulandari","doi":"10.15578/SQUALEN.497","DOIUrl":"https://doi.org/10.15578/SQUALEN.497","url":null,"abstract":"Fermented fish, including rusip and bekasam , contains lactic acid bacteria, some of which have probiotic properties. Considering the beneficial effect, the count of viable cells in probiotic carrier foods must be at least 10 6 CFU/g throughout the product shelf-life. The processing and storage condition significantly affect the viability of probiotics in carrier foods. This research was conducted to determine the heat resistance of probiotic candidate Enterococcus faecalis R22B isolated from rusip in three different media: bekasam, rusip, and physiological saline solution (NaCl 0.85%). E. faecalis R22B was heated in each medium at a temperature of 70 °C for 0, 2, 4, 8, and 12 min. The media characteristics showed differences within the parameters included pH values of 4.41-6.99; a w 0.67-0.92; moisture content 72.56-98.09%; ash content 0.69-12.32%; protein content 0-14.7%; fat content 0-0.84%; and carbohydrate content from 0-4.93%. The results showed that the difference in the heating medium had no significant effect ( p >0.05) on the heat resistance of the bacteria, as indicated by the D 70 value. The required average heating time at 70 o C to decrease E. faecalis R22B cells by 1log 10 (D 70 value) in bekasam, rusip, and physiological saline media were 3.17, 3.29, and 3.55 min, respectively. Despite having a lower D T -value, the reduction of bacteria cells in three media heated at 70 o C showed no difference ( p >0.05); therefore, bekasam and rusip are suitable for probiotic carrier food.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90778050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0