Skin pharmacology : the official journal of the Skin Pharmacology Society最新文献

Pro-inflammatory cytokine cascade in human plucked hair. 人拔毛中的促炎细胞因子级联。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-09-01 DOI: 10.1016/S0926-9959(97)89373-7

Y. Mahé, B. Buan, N. Billoni, G. Loussouarn, J. Michelet, B. Gautier, B. Bernard

引用次数: 29

Activity and inhibition of 3-beta-hydroxysteroid dehydrogenase/delta-5-4-isomerase in human skin. 3- β -羟基类固醇脱氢酶/ δ -5-4异构酶在人体皮肤中的活性及抑制作用。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211481

I Tóth, M Szécsi, J Julesz, I Faredin

引用次数: 14

Sebaceous-gland deposition of isotretinoin after topical application: an in vitro study using human facial skin. 局部应用后异维甲酸的皮脂腺沉积:使用人类面部皮肤的体外研究。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211477

T Tschan, H Steffen, A Supersaxo

{"title":"Sebaceous-gland deposition of isotretinoin after topical application: an in vitro study using human facial skin.","authors":"T Tschan, H Steffen, A Supersaxo","doi":"10.1159/000211477","DOIUrl":"https://doi.org/10.1159/000211477","url":null,"abstract":"As yet, topically applied isotretinoin fails to show convincing clinical efficacy in the treatment of severe recalcitrant acne. Although the reason for this is not known, it is possible that topical application results in low, pharmacologically inactive isotretinoin concentrations in the sebaceous glands, the most likely site of isotretinoin action. It has been suggested that topically applied liposomes enhance the delivery of drugs into the sebaceous glands. Accordingly, we compared the isotretinoin concentration in sebaceous glands and other skin compartments following topical application of small unilamellar vesicles, multilamellar vesicles, preformed vesicles (Natipide II) or mixed micelles of lecithin and bile salt. We found that the concentration of isotretinoin measured in the sebaceous glands varied between 0.17 and 1.57 ng/mg tissue. The comparison between ethanolic gel and liposomal or micellar gel did not reveal any significant difference. However, application of the Natipide formulation resulted in significantly lower isotretinoin concentrations in the sebaceous glands when compared to the ethanolic gel. Autoradiography and fluorescence microscopy indicated that isotretinoin penetrated the sebaceous glands along the follicular route. In conclusion, our in vitro study showed that, following topical administration, substantial amounts of isotretinoin were delivered to the sebaceous glands via the follicular route, whereby the ethanolic gel was as efficient as a liposomal or a mixed micellar gel.","PeriodicalId":21596,"journal":{"name":"Skin pharmacology : the official journal of the Skin Pharmacology Society","volume":"10 3","pages":"126-34"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000211477","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20227493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

In vitro susceptibility testing of Malassezia furfur against rilopirox. 毛马拉色菌对利洛匹罗的体外药敏试验。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211515

Pietro Nenoff, Uwe-Frithjof Haustein

{"title":"In vitro susceptibility testing of Malassezia furfur against rilopirox.","authors":"Pietro Nenoff, Uwe-Frithjof Haustein","doi":"10.1159/000211515","DOIUrl":"https://doi.org/10.1159/000211515","url":null,"abstract":"The in vitro antifungal activity of the new hydroxypyridone antimycotic rilopirox has been evaluated against 29 separate clinical isolates of Malassezia (M.) furfur obtained from patients with pityriasis versicolor, seborrhoeic dermatitis or dandruff. Minimum inhibitory concentrations (MICs) of rilopirox were measured by the agar dilution technique and, in comparison, by a recently described microdilution method with colorimetric detection of the MIC end points. Rilopirox was found to be able to inhibit growth of all clinical yeast isolates. For the investigated M. furfur strains MIC values from 12.5 to 50 micrograms ml-1 with a median of 25 micrograms ml-1 were determined by the agar dilution method. Using the microdilution technique, MIC values between 16 and 128 micrograms ml-1 (median 32 micrograms ml-1) were found for the M furfur isolates. It has to be taken into account that with a 0.3% solution concentrations of 300,000 micrograms ml-1 are applied to the skin. Furthermore, due to its extreme low penetration rilopirox is long-term available in the skin in inhibiting concentrations. In comparison with rilopirox, the in vitro susceptibility of M. furfur against the systemically applicable triazole antimycotic itraconazole and clotrimazole, an established topical antifungal, was tested. As expected, low MIC values for these azoles were found by the agar dilution method. The median of the MIC of M. furfur was 0.1 microgram ml-1 for itraconazole, and 6.25 micrograms ml-1 for clotrimazole. The inhibitory effectivity of rilopirox against clinical isolates of M. furfur seems to justify its therapeutic evaluation in clinical trials. This new antifungal may be a useful alternative not only in pityriasis versicolor but also in seborrhoeic dermatitis due to the growth inhibition of M. furfur.","PeriodicalId":21596,"journal":{"name":"Skin pharmacology : the official journal of the Skin Pharmacology Society","volume":"99 1","pages":"275-80"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85863079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Barrier function of reconstructed epidermis at the air-liquid interface: influence of dermal cells and extracellular components. 重建表皮在气液界面的屏障功能:真皮细胞和细胞外成分的影响。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211512

M Robert, I Dusser, M P Muriel, M S Noël-Hudson, M Aubery, J Wepierre

引用次数: 15

DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol. 全反式维甲酸和视黄醇诱导的犀牛小鼠皮肤DNA倍体变化。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211478

S González, M V Alcaraz, F Díaz, T J Flotte, I Pérez de Vargas, R R Anderson, N Kollias

{"title":"DNA ploidy changes in rhino mouse skin induced by all-trans retinoic acid and retinol.","authors":"S González, M V Alcaraz, F Díaz, T J Flotte, I Pérez de Vargas, R R Anderson, N Kollias","doi":"10.1159/000211478","DOIUrl":"https://doi.org/10.1159/000211478","url":null,"abstract":"Objective: In order to assess the proliferative changes induced by all-trans retinoic acid (RA) and retinol (ROL), we have carried out a study of the DNA content of basal and suprabasal keratinocytes after epicutaneous application on the rhino mouse.Study design: Skin sections were analyzed stereologically and cytophotometrically using the Feulgen technique. The diploid DNA value (2C) was obtained from hepatocyte nuclei of control animals. Whereas cells in phase G0-G1 will show a 2C content, cells during phase S and in phase G2-M will show DNA values ranging from 2C to 4C and 4C, respectively.Results: Although epidermal thickness (ET) increased significantly in all treated animals, surface density only increased in animals treated with all-trans RA. Quantification of DNA content of basal keratinocytes showed reduction of 2C and 2C-4C populations with a commensurate increase in proportions of cells with 4C and > 4C in the animals treated with 0.025% all-trans RA and ROL. Suprabasal keratinocytes of mice treated with 0.025% all-trans showed a decrease of the 2C population and an increased proportion of cells with 4C. Whereas 0.025% all-trans RA induced an increase of both basal and suprabasal DNA indices, ROL enhanced only the basal DNA index significantly.Conclusion: Animals treated with 0.025% ROL showed a significant increase in the basal proliferative index (PI) while the suprabasal PI remained constant; treatment with 0.025% all-trans RA produced a significant increase of both basal and suprabasal PIs and parakeratotic hyperkeratosis probably due to incomplete differentiation.","PeriodicalId":21596,"journal":{"name":"Skin pharmacology : the official journal of the Skin Pharmacology Society","volume":"10 3","pages":"135-43"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000211478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20227344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Immunological gene therapy approaches for malignant melanoma. 2. Preclinical studies and clinical strategies. 恶性黑色素瘤的免疫基因治疗方法。2. 临床前研究和临床策略。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211476

B Bonnekoh, J R Bickenbach, D R Roop

{"title":"Immunological gene therapy approaches for malignant melanoma. 2. Preclinical studies and clinical strategies.","authors":"B Bonnekoh, J R Bickenbach, D R Roop","doi":"10.1159/000211476","DOIUrl":"https://doi.org/10.1159/000211476","url":null,"abstract":"Immuno-gene therapy approaches for the treatment of malignant melanoma are categorized into two major subgroups according to an active or passive immunological principle. Active immuno-gene therapy is subdivided into melanoma cell vaccines, DNA-based vaccinations and the treatment of pre-existing tumor tissue by cell-mediated or direct transfer of cytokine and/or cell surface signal genes. Passive immuno-gene therapy, employing an adoptive treatment with in vitro activated and expanded anti-tumor effector cells, involves two major application fields for gene transfer techniques, first the genetic modification of the effector cells, and second the in vivo amplification of pre-effector cells by procedures also used in active immuno-gene therapy. Corresponding preclinical studies are reviewed. The clinical studies inaugurated during the last few years are mostly still ongoing and focus on treatment safety and tolerability rather than efficacy. A recent trend is emerging to explore recombinant adenovirus and vaccinia virus vectors particularly with regard to in vivo gene transfer applications. Overall, immuno-gene therapy of melanoma is still in a highly experimental stage of development but may become a safe, efficacious and practical adjuvant treatment modality in the future.","PeriodicalId":21596,"journal":{"name":"Skin pharmacology : the official journal of the Skin Pharmacology Society","volume":"10 3","pages":"105-25"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000211476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20227492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Inhibition of skin protein kinase C by psychotropic drugs. 精神药物对皮肤蛋白激酶C的抑制作用。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211504

R Vaitla, P Roshani, O Holian, B Cook, R Kumar

{"title":"Inhibition of skin protein kinase C by psychotropic drugs.","authors":"R Vaitla, P Roshani, O Holian, B Cook, R Kumar","doi":"10.1159/000211504","DOIUrl":"https://doi.org/10.1159/000211504","url":null,"abstract":"Lipid-soluble psychotropics are often used to treat skin diseases with psychosomatic indications. Although these drugs are known to exert their effects through the central nervous system, relatively little is known about their mechanism of action in skin. In this communication, several lipid-soluble psychotropic drugs have been examined for their ability to inhibit protein kinase C (PKC)-catalyzed phosphorylation of exogenous substrates and endogenous skin proteins. Phosphorylation of three discrete skin protein substrates at 64, 42 and 28 kDa and a group crowded together at 15-18 kDa was prevented by the antidepressants/antipsychotics. Inhibition was more pronounced in a phospholipid (PL) dependent system, but both drug-PL and drug-PKC interactions seem to be important in the mechanism of action of these drugs. In addition to the tricyclic nucleus, the propanamine side chain or its N-methyl form may influence the interaction of these drugs with PKC and its substrate(s). Chlorpromazine, imipramine, fluoxetine, doxepin, amitriptyline and hydroxyzine used in the practice of dermatology may exert their therapeutic effects by modulating skin PKC activity.","PeriodicalId":21596,"journal":{"name":"Skin pharmacology : the official journal of the Skin Pharmacology Society","volume":"10 4","pages":"191-9"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000211504","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20343238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Effect of topical corticosteroids on the activity of superoxide dismutase in human skin in vitro. 外用皮质类固醇对体外人皮肤超氧化物歧化酶活性的影响。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211520

N Gavan, H Maibach

引用次数: 15

Immunological gene therapy approaches for malignant melanoma. 1. Tumor-immunological background. 恶性黑色素瘤的免疫基因治疗方法。1. Tumor-immunological背景。

Skin pharmacology : the official journal of the Skin Pharmacology Society Pub Date : 1997-01-01 DOI: 10.1159/000211469

B Bonnekoh, J R Bickenbach, D R Roop

引用次数: 9