Sains Malaysiana最新文献_第8页

Bootstrap Methods for Estimating the Confidence Interval for the Parameter of the Zero-Truncated Poisson-Sujatha Distribution and their Applications 估计零截断Poisson-Sujatha分布参数置信区间的Bootstrap方法及其应用

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-22

W. Panichkitkosolkul

引用次数: 0

Persicaria minor F-box Gene Pmf-box1 Indirectly Affects Arabidopsis thaliana LOX-HPL Pathway for Green Leaf Volatile Production 小桃F-box基因Pmf-box1间接影响拟南芥LOX-HPL叶片挥发物生成途径

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-04

Nur Athirah Abd Hamid, Muhammad Naeem ul Hassan, Z. Zainal, I. Ismail

{"title":"Persicaria minor F-box Gene Pmf-box1 Indirectly Affects Arabidopsis thaliana LOX-HPL Pathway for Green Leaf Volatile Production","authors":"Nur Athirah Abd Hamid, Muhammad Naeem ul Hassan, Z. Zainal, I. Ismail","doi":"10.17576/jsm-2023-5206-04","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-04","url":null,"abstract":"Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation of genes involved in the lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway, which contributes to the biosynthesis of GLVs. It resulted in a marked reduction of hexanal production in the PmF-box1-overexpressing plant. The expression pattern of LOX-HPL branch genes in the kelch-repeat modified PmF-box1 (KMF)-overexpressing plant showed a pattern much closer to the expression of LOX-HPL branch genes in the vector control (VC) plant. It was shown that the functional KMF protein sequence was not responsible for the significant reduction of all GLVs including hexanal, 1-hexanol, (Z)-3-hexen-1-ol, and the carbon 5 (C5) volatile, 1-penten-3-ol, in plants overexpressing KMF. Furthermore, this study also showed that the relative proportion of production of 1-penten-3-ol to hexanal was higher in the PmF-box1-overexpressing plant. Based on the current comparative literature search, PmF-box1 does not appear to interact directly with the proteins or transcription factors of the LOX-HPL pathway. On the other hand, PmF-box1 interacts with SAMS1, which subsequently influences the HPL pathway enzyme genes. Thus, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41669960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physical Properties of Tapioca Starch-based Film Indicators with Anthocyanin Extract from Purple Sweet Potato (Ipomea batatas L.) and Response to pH Changes 紫薯花青素提取物对木薯淀粉基薄膜指示剂的物理性质及对pH变化的响应

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-06

Safinta Nurindra Rahmadhia Safinta Nurindra Rahmadhia, Afist Azkiya Sidqi Afist Azkiya Sidqi, Yanas Anggana Saputra Yanas Anggana Saputra

{"title":"Physical Properties of Tapioca Starch-based Film Indicators with Anthocyanin Extract from Purple Sweet Potato (Ipomea batatas L.) and Response to pH Changes","authors":"Safinta Nurindra Rahmadhia Safinta Nurindra Rahmadhia, Afist Azkiya Sidqi Afist Azkiya Sidqi, Yanas Anggana Saputra Yanas Anggana Saputra","doi":"10.17576/jsm-2023-5206-06","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-06","url":null,"abstract":"Intelligent packaging comes in the form of interactive film indicator using a natural pigment compound sensitive to pH changes. The development of intelligent packaging as an indicator film by utilizing natural pigment compounds that are related to pH change and food safety is motivated by increased consumer awareness of food safety. Purple sweet potato (Ipomoea batatas L.) is the source of anthocyanin flavonoid compounds sensitive to pH changes, demonstrated by color changes in film indicators. This research aims to determine physical properties and the pH response of tapioca starch-based film indicators with anthocyanin extract variation from purple sweet potato. Purple sweet potato anthocyanin (PSPA) indicator film was made using tapioca starch as biopolymer by casting method with the addition of anthocyanin extract at concentrations of 0, 5, 10, and 15 g. Furthermore, this research is conducted to analyze the physical properties of the film, and response to pH changes of fresh cow milk, Gindara fish fillet and chicken sausage stored at 7 °C and 25 °C under 48-hour observation. The results showed that the film indicator thickness was 0.72-0.74 mm, tensile strength was 1.23-9.86 MPa, elongation was 14.83-55.74%, and water vapor permeability (WVP) was 1.32-1.78 × 10-14 kg.m/m2.s.Pa. The results of this study indicated that the PSPA indicator films have the potential to be used as smart packaging to monitor food freshness and quality for safe consumption. That was supported by the good physical properties of PSPA indicator films.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43904610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Effect of Liquid Smoke Treatment on Physical Stability and Impact Toughness Chicken Feather Fiber (CFF) as Reinforcement in Composites 液烟处理对鸡毛纤维增强复合材料物理稳定性和冲击韧性的影响

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-19

Mukhlis Muslimin Mukhlis Muslimin, Saleh N. Saleh, Ahmad Seng, Willy Artha Wirawan, Mohammad Muzni harbelubun, Sandi Rais, Muhammad Alfajri Isham Muhammad Alfajri Isham

{"title":"The Effect of Liquid Smoke Treatment on Physical Stability and Impact Toughness Chicken Feather Fiber (CFF) as Reinforcement in Composites","authors":"Mukhlis Muslimin Mukhlis Muslimin, Saleh N. Saleh, Ahmad Seng, Willy Artha Wirawan, Mohammad Muzni harbelubun, Sandi Rais, Muhammad Alfajri Isham Muhammad Alfajri Isham","doi":"10.17576/jsm-2023-5206-19","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-19","url":null,"abstract":"The objective of this study was to determine the effect of liquid smoke treatment on chicken feather fibres (CFF) on the morphology and impact toughness of composites reinforced with CFF. The experiment was carried out by measuring the density of CFF, followed by immersion in liquid smoke for 1, 2, and 3 hours. All of the fibers, including one without the immersion treatment, were then inserted into the oven at a temperature of 40 °C for 30 min. The morphological assessments were done using a scanning electron microscope (SEM) and the impact test according to the ASTM D5942-96 standard on the Charpy method impact tester. The SEM results showed that the fibers without immersion had a multitude of impurities at the surface, while with immersion treatment, the fibers had a more clean and rough surface. Impact test results showed that the fiber-reinforced composite without treatment was 61,583 Kj/m2, while the fiber-reinforced composite with immersion treatment for 1, 2, and 3 hours increased by 63,894 Kj/m2, 71,061 Kj/m2, and 80,538 Kj/m2. Treatment for 3 hours significantly increased the strength of the composite by 30.78%.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46357175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Kinetic Study of Total Phenolic Content from Piper betle Linn. Leaves Extract Using Subcritical Water 菟丝子总酚含量的动力学研究。用亚临界水提取叶子

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-10

N. Rahmah, S. M. Mustapa Kamal, A. Sulaiman, F. Taip, S. I. Siajam

{"title":"Kinetic Study of Total Phenolic Content from Piper betle Linn. Leaves Extract Using Subcritical Water","authors":"N. Rahmah, S. M. Mustapa Kamal, A. Sulaiman, F. Taip, S. I. Siajam","doi":"10.17576/jsm-2023-5206-10","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-10","url":null,"abstract":"The green plant-based extraction of phenolic compounds is still challenging and attractive due to their benefit. The mechanism controlling of desorption rate of phenolic compounds, measured as total phenolic content (TPC), from Piper betle Linn. (PBL) leaves using subcritical water, and a one-site kinetic desorption model (first order) was studied. One-site kinetic desorption model has well explained the extraction mechanism of phenolic compounds from PBL leaves using subcritical water through desorption and diffusion mechanism. This model fits with the experimental data and presents a good description of the extraction mechanism with R-squared of 0.94. The recovery of TPC from PBL leaves using subcritical water was influenced by intraparticle diffusion, temperature, and extraction time. The desorption rate constant in the one-site kinetic desorption model increased from 100 to 200 °C (0.3975±0.02 to 3.3045±0.00 min-1) and then decreased to 250 °C (3.2093±0.00 min-1). The highest TPC was recovered quickly for 5 min at 200 °C. In addition, a high yield of TPC was also obtained at a slow desorption process for 30 min at a lower temperature of 175 °C. The low activation energy for the diffusion of phenolic compounds from PBL leaves of this study was 8.964 kJ/mol. This result showed that the one-site kinetic desorption model of subcritical water extraction has an excellent opportunity to be applicable in phenolic compounds recovery from PBL leaves. The one-site kinetic desorption rate constant and mathematical kinetic model equation achieved in this study might control the quality of phenolic compounds extracted from PBL leaves through subcritical water.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45547971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Antidiabetic Effect of Lupenone in Rats with Type 1 Diabetes and its Underlying Mechanism Based on Network Pharmacology 基于网络药理学的Lupenone对1型糖尿病大鼠的降糖作用及其机制研究

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-15

Hongmei Wu, Xulong Huang, Feng Xu, Xiaofen Li, Yuqing Liang, Liubo Yang, Xiangpei Wang

{"title":"Exploring the Antidiabetic Effect of Lupenone in Rats with Type 1 Diabetes and its Underlying Mechanism Based on Network Pharmacology","authors":"Hongmei Wu, Xulong Huang, Feng Xu, Xiaofen Li, Yuqing Liang, Liubo Yang, Xiangpei Wang","doi":"10.17576/jsm-2023-5206-15","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-15","url":null,"abstract":"Lupenone has been reported to possess numerous medicinal values and gives a positive antidiabetic effect. But the mechanism of preventing and treating type 1 diabetes has not been elucidated in type 1 diabetic rats. This study investigated the effects and mechanism of action of lupenone in preventing and treating type 1 diabetes by network pharmacology and diabetic rats. The blood glucose, glycosylated hemoglobin (HbA1c), insulin, and inflammatory factors in the pancreas of rats with type 1 diabetes were measured, and histopathological changes were observed after treatment with lupenone. The pharmacological network of ‘component-target-disease’ was constructed on diabetic rats. Gene function enrichment, the Kyoto Encyclopedia of Genes and Genomes pathway analysis, and molecular docking were performed. The results showed that lupenone can decrease fasting blood glucose and HbA1c levels, increase insulin content and interleukin (IL)-4, IL-10, and decrease IL-6, transforming growth factor β and tumor necrosis factor α levels in the pancreas. Furthermore, ten targets were identified, and 50 signal pathways closely related to type 1 diabetes and inflammation were screened by network pharmacology, including insulin resistance, type II diabetes, type I diabetes, insulin signal pathway, mitogen activated protein kinase (MAPK) signal pathway, and tumor necrosis factor (TNF) signal pathway. The docking affinity of potential targets and lupenone were between -3.3 and -9.8, among which caspase-3 (CASP3), cyclin-dependent kinase 4 (CDK4), inhibitor of kappaB kinase beta (IKBKB), transforming growth factor beta-1 (TGFB1), and TNF had high binding abilities. Thus, lupenone has the potential to be developed as a new drug for treating type 1 diabetes.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43913501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pemeringkatan Saham Patuh Syariah Menggunakan Pembuatan Keputusan Multi-Kriterium: TOPSIS dan GRA 正在同步文件夹

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-21

Farah Azaliney Binti Mohd Amin, Saiful Hafizah Jaaman

{"title":"Pemeringkatan Saham Patuh Syariah Menggunakan Pembuatan Keputusan Multi-Kriterium: TOPSIS dan GRA","authors":"Farah Azaliney Binti Mohd Amin, Saiful Hafizah Jaaman","doi":"10.17576/jsm-2023-5206-21","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-21","url":null,"abstract":"Peningkatan bilangan saham patuh Syariah yang didagangkan di Bursa Malaysia menjadikan proses pemilihan pelaburan semakin penting dan mencabar. Oleh itu, pelabur memerlukan suatu mekanisme bersifat kuantitatif dan bukan hanya berdasarkan penilaian kualitatif atau intuitif semata-mata. Dalam kajian ini, prestasi saham 10 syarikat patuh Syariah yang disenaraikan oleh Suruhanjaya Sekuriti Malaysia (SCM) dianalisis menggunakan nisbah kewangan utama syarikat iaitu pelaburan, keberuntungan, kecairan, keumpilan dan kecekapan. Fokus kajian adalah untuk memeringkatkan prestasi saham dari 2017 sehingga 2021 menggunakan dua kaedah iaitu Teknik Keutamaan Tertib Mengikut Persamaan dengan Penyelesaian Ideal (TOPSIS) dan Analisis Hubungan Kelabu (GRA). Keputusan kajian menunjukkan konsistensi pemeringkatan saham antara kedua-dua kaedah, terutama dalam memilih dua kedudukan teratas iaitu NESTLE dan PETGAS serta tiga saham kedudukan terbawah iaitu MISC, PMETAL dan AXIATA. Keputusan ini menunjukkan bahawa GRA merupakan kaedah alternatif yang setanding dengan TOPSIS dan mampu membantu para pelabur untuk mengenal pasti saham patuh Syariah terbaik.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47751938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pemetaan Geobahaya Berdasarkan Kaedah Faktor Penilaian Tanah Runtuh (LHEF), di Empangan Sultan Mahmud, Tasik Kenyir, Kuala Berang, Terengganu

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-23

Mohamad Anuri Ghazali, Mohd Rozi Umor, Abdul Ghani Md Rafek

{"title":"Pemetaan Geobahaya Berdasarkan Kaedah Faktor Penilaian Tanah Runtuh (LHEF), di Empangan Sultan Mahmud, Tasik Kenyir, Kuala Berang, Terengganu","authors":"Mohamad Anuri Ghazali, Mohd Rozi Umor, Abdul Ghani Md Rafek","doi":"10.17576/jsm-2023-5206-23","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-23","url":null,"abstract":"Kajian pemetaan potensi tanah runtuh telah dilakukan dengan menggunakan kaedah Faktor Penilaian Tanah Runtuh (LHEF) di Empangan Sultan Mahmud, Tasik Kenyir yang melibatkan kawasan tanah tinggi dengan litologi batuan granit, cerun potong kejuruteraan dan cerun semula jadi. Kaedah pemetaan LHEF sangat berguna kepada perancang bandar bagi tujuan pembangunan kawasan untuk memastikan secara relatif kawasan tersebut selamat. Permasalahan dalam kajian ini adalah kawasan kajian yang berbukit dan berbatu yang curam yang mana agak sukar untuk memperoleh data. Sebanyak enam (6) parameter dipertimbangkan dalam penilaian geobahaya cerun iaitu litologi, struktur geologi, morfometri cerun, penurunan relatif, guna tanah dan tutupan tanah serta keadaan hidrogeologi. Hasil daripada penilaian yang dijalankan diplotkan di dalam peta geobahaya potensi tanah runtuh. Penentuan kaedah pemetaan geobahaya sesuatu kawasan perlu mengambil kira keadaan sesuatu bagi memastikan pemetaan geobahaya yang dijalankan menghasilkan keputusan yang tepat. Sebanyak 858 cerun telah dinilai di dalam kajian ini yang merangkumi kawasan kajian 1, 2 dan 3. Didapati699 cerun dizonkan sebagai geobahaya sederhana, 87 cerun dizonkan sebagai geobahaya tinggi dan hanya 72 cerun dizonkan sebagai geobahaya rendah. Secara asasnya ciri cerun yang dikategori sebagai geobahaya rendah adalah landai, tiada hakisan dan tiada struktur geodinamik. Zon geobahaya cerun sederhana pula adalah cerun yang mempunyai kecerunan sederhana curam, hakisan alur dan galur. Cerun yang dikategori sebagai geobahaya tinggi mempunyai struktur geodinamik seperti tegangan, rekahan, kecerunan melebihi 45 darjah, batu tongkol yang longgar, runtuhan lama, hakisan dan ketakselanjaran yang recam.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44380089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods of Evaluating Adaptation and Accuracy of Additive Manufactured Removable Partial Dentures: A Scoping Review 评价增材制造可摘局部义齿的适应性和准确性的方法:范围综述

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-12

Safa Elhadery, N. Yacob, S. Safii, Norliza Ibrahim, Zubaidah Zanul Abidin, Nosizana Mohd Salleh

{"title":"Methods of Evaluating Adaptation and Accuracy of Additive Manufactured Removable Partial Dentures: A Scoping Review","authors":"Safa Elhadery, N. Yacob, S. Safii, Norliza Ibrahim, Zubaidah Zanul Abidin, Nosizana Mohd Salleh","doi":"10.17576/jsm-2023-5206-12","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-12","url":null,"abstract":"This study aimed to provide a comprehensive review of various recent methods that can be used to assess the fit and accuracy of additive-manufactured removable partial dentures (RPDs), focusing on 3D-printed RPDs. An electronic search of the English language literature from January 2000 to February 2022 was performed using four databases: Medline/PubMed, Scopus, Web of Science, and EBSCOhost, using relevant keywords. The parameters of interest were extracted and tabulated. Of 936 retrieved studies, 26 studies were included. Most of the studies were laboratory studies, conducted between 2011 and 2022, did not include control group, used stone cast model as reference, used direct 3D printing method, and polished the final RPD framework. Methods of assessment can be divided into two categories: 1) qualitative assessment which is based mainly on visual inspection or tactile sense, and 2) quantitative assessment which includes optical assessment (with or without a registration material) and computerized assessment based on surface-matching software programs. In conclusion, computerized assessment using different surface matching software provides more accurate and precise quantitative assessment of denture fit and allows researcher and practitioner to detect minute dimensional changes that cannot be detected visually.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44578736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro and in Silico Study on the Interaction between Apigenin, Kaempferol and 4-Hydroxybenzoic Acid in Xanthine Oxidase Inhibition 芹菜素、山奈酚和4-羟基苯甲酸对黄嘌呤氧化酶抑制作用的体外和室内研究

IF 0.8 4区综合性期刊

Sains Malaysiana Pub Date : 2023-06-30 DOI: 10.17576/jsm-2023-5206-03

Yong Sin Chin, K. Loh, Sze Ping Wee, G. H. Ong

{"title":"In Vitro and in Silico Study on the Interaction between Apigenin, Kaempferol and 4-Hydroxybenzoic Acid in Xanthine Oxidase Inhibition","authors":"Yong Sin Chin, K. Loh, Sze Ping Wee, G. H. Ong","doi":"10.17576/jsm-2023-5206-03","DOIUrl":"https://doi.org/10.17576/jsm-2023-5206-03","url":null,"abstract":"Xanthine oxidase (XO) is a biological enzyme that takes part in purine catabolism. It catalyses the conversion of hypoxanthine to xanthine and eventually xanthine to uric acid. The catabolism reaction increases the level of uric acid and subsequently leads to hyperuricemia. Allopurinol is a XO inhibitor that is used clinically to prevent purine catabolism. Although it is an effective XO inhibitor, it causes some side effects. Therefore, a more effective inhibitor with fewer side effects is in an urgent need. Phenolic compounds have been identified as effective XO inhibitors in many studies. In vitro and in silico study were conducted to investigate the interaction between apigenin, kaempferol and 4-hydroxybenzoic acid in XO inhibition. Apigenin was found to be the most effective XO inhibitor among the compounds tested with the best docking score of -8.2 kcal/mol as demonstrated in the molecular docking simulation which indicated its favourable interaction with XO enzyme. Additive interactions between compounds namely apigenin-kaempferol, apigenin-4-hydroxybenzoic acid and 4-hydroxybenzoic acid-kaempferol were demonstrated in both in vitro and in silico studies. The results showed that 4-hydroxybenzoic acid- apigenin (-7.4 kcal/mol) was the most stable ligands combination docked to XO. The multiple ligands docking simulation showed independent ligands bound to the XO active site at non-interfering regional location. In conclusion, the combination of these three compounds can be explored further for their additive interaction in XO inhibition, which could be beneficial in terms of the enhanced effectiveness and lower side effects when each is used at lower dose to give the same effect.","PeriodicalId":21366,"journal":{"name":"Sains Malaysiana","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46533655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0