Reproduction, nutrition, development最新文献_第8页

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development. 生长激素受体基因在正常和双肌牛胎儿发育期间骨骼肌中的表达。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005036

Anne Listrat, Jean François Hocquette, Brigitte Picard, François Ménissier, Jean Djiane, Hélène Jammes

{"title":"Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development.","authors":"Anne Listrat, Jean François Hocquette, Brigitte Picard, François Ménissier, Jean Djiane, Hélène Jammes","doi":"10.1051/rnd:2005036","DOIUrl":"https://doi.org/10.1051/rnd:2005036","url":null,"abstract":"The expression of the growth hormone receptor (GHR) gene was investigated in semitendinosus muscle during bovine foetal development in both normal and double-muscled Charolais foetuses which differ with respect to muscle development. Northern-blot analysis of foetal muscle RNA preparations with a GHR cDNA probe identified the 4.5 kb GHR mRNA as early as 130 days post-conception. In double-muscled animals, the expression of GHR mRNA increased from 130 to 210 days of gestation while it stayed stable in normal ones. It was significantly higher (P < 0.05) in double-muscled foetuses compared to normal ones from the second third of gestation. Northern-blot analysis of foetal muscle RNA preparations from both genotypes with a beta-actin cDNA probe, revealed lower beta-actin gene expression in double-muscled foetuses than in normal ones, suggesting a delay in the differentiation of muscle cells. In situ hybridisation revealed the localisation of specific GHR mRNA in muscle cells at all gestation stages analysed (130, 170, 210 days post-conception) but not in connective tissue surrounding the muscle cells. At the adult stage, the hybridisation signal was also very high and observed in muscle cells only. These results show the ontogeny of GHR mRNA in bovine muscle and demonstrate a difference between normal and double-muscled animals.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"393-403"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25213966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Effect of feeding live yeast products to calves with failure of passive transfer on performance and patterns of antibiotic resistance in fecal Escherichia coli. 被动转移失败犊牛饲喂活酵母产品对犊牛生产性能和粪便大肠杆菌耐药模式的影响

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005040

Klibs N Galvão, José E P Santos, Anelis Coscioni, Marcos Villaseñor, William M Sischo, Anna Catharina B Berge

{"title":"Effect of feeding live yeast products to calves with failure of passive transfer on performance and patterns of antibiotic resistance in fecal Escherichia coli.","authors":"Klibs N Galvão, José E P Santos, Anelis Coscioni, Marcos Villaseñor, William M Sischo, Anna Catharina B Berge","doi":"10.1051/rnd:2005040","DOIUrl":"https://doi.org/10.1051/rnd:2005040","url":null,"abstract":"Fifty-two newborn Holstein calves with serum IgG concentrations less than 0.73 g.dL(-1) were randomly assigned to one of four treatments: no added live yeast (control), 0.5 g of live yeast added to the grain for 84 d (SC; Saccharomyces cerevisiae), 0.5 g of live yeast added to the milk for 42 d (SB; S. cerevisiae, spp. boulardii), and 0.5 g of live yeast added to the grain for 84 d and to the milk for 42 d (SCSB). Calves were offered 440 g of milk replacer DM for the first 42 d and grain for ad libitum intake throughout the study. Plasma was analyzed weekly for concentrations of glucose and beta-hydroxybutyrate. Escherichia coli isolated from fecal samples collected every 2 weeks were used for determination of antibiotic resistance patterns. Calves receiving SC consumed more grain DM, had increased weight gain prior to weaning, and increased plasma glucose concentrations compared to controls. Days with diarrhea were reduced by feeding live yeast to calves. Antibiotic resistance in fecal E. coli was associated with the age of calves with highest levels of resistance observed in the 3 d calves. While calves receiving SCSB had higher levels of antibiotic resistance than controls, this effect was not associated with any of the other treatments. Improvements in performance of calves with failure of passive transfer were observed when live yeast was added only to the grain.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"427-40"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25213969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 138

The minerals of milk. 牛奶中的矿物质。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005030

Frédéric Gaucheron

引用次数: 525

Nutritional value of meat: the influence of nutrition and physical activity on vitamin B12 concentrations in ruminant tissues. 肉的营养价值:营养和身体活动对反刍动物组织中维生素B12浓度的影响。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005038

Isabelle Ortigues-Marty, Didier Micol, Sophie Prache, Dominique Dozias, Christiane L Girard

{"title":"Nutritional value of meat: the influence of nutrition and physical activity on vitamin B12 concentrations in ruminant tissues.","authors":"Isabelle Ortigues-Marty, Didier Micol, Sophie Prache, Dominique Dozias, Christiane L Girard","doi":"10.1051/rnd:2005038","DOIUrl":"https://doi.org/10.1051/rnd:2005038","url":null,"abstract":"An important nutritional characteristic of ruminant meat is its high content in vitamin B12. The variability of these contents is not known. Three studies were been set up in order to test the influence of the animal species (2 studies on Charolais steers slaughtered at 30-32 months of age, n = 24 and n = 30 and a third one on lambs slaughtered at 4.5 months of age, n = 21), of the nature of the diet (grass vs. maize silage, lucerne or concentrate diets) and of physical activity (without or with walking) on the vitamin B12 contents of different muscle types (rather oxidative (Rectus Abdominis, RA), intermediate (Longissimus Dorsi, LD), or glycolytic (Semi Tendinosus, ST)) and on the liver. The animals were supplemented in macro and trace minerals according to usual feeding practices in France in order to theoretically avoid any risk of deficiency. For this reason, cobalt allowances, which are necessary for the ruminal synthesis of vitamin B12, could differ among treatments. The results indicate the following: (1) cobalt allowances varied widely among treatments, from (sub-)deficient to plethoric allowances, influencing vitamin B12 contents of the liver, and muscles (only in case of deficiency), (2) the effects of dietary treatments or of physical exercise were essentially related to differences in cobalt allowances, (3) the oxidative type muscle (RA) showed contents which were double those in glycolytic type muscle (RA 10.8 vs. ST 5.0 ng.g(-1)) and (4) vitamin B12 contents of raw muscles were lower than the values indicated in tables of feed composition for humans for cooked meat (0.5 to 1 vs. 2 to 3 microg.100 g(-1)).","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"453-67"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25213973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Testing the antagonistic effect of follistatin on BMP family members in ovine granulosa cells. 卵泡抑素对绵羊颗粒细胞BMP家族成员的拮抗作用。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005031

Alice Pierre, Claudine Pisselet, Philippe Monget, Danielle Monniaux, Stéphane Fabre

{"title":"Testing the antagonistic effect of follistatin on BMP family members in ovine granulosa cells.","authors":"Alice Pierre, Claudine Pisselet, Philippe Monget, Danielle Monniaux, Stéphane Fabre","doi":"10.1051/rnd:2005031","DOIUrl":"https://doi.org/10.1051/rnd:2005031","url":null,"abstract":"Follistatin was first demonstrated as an activin-binding protein, neutralizing its actions. However, there is emerging evidence that follistatin inhibits the action of other members of the transforming growth factor beta(TGFbeta) / bone morphogenetic protein (BMP) superfamily. Recently, numerous BMP factors have been shown to play important roles in regulating folliculogenesis and ovulation rate in mammals, and such a potential antagonistic role of follistatin is of particular interest in the context of ovarian function. Using a biological test based on progesterone production by ovine primary granulosa cells in culture, we show that follistatin was a strong antagonist of activin A, but not BMP-2 or BMP-4 actions. In contrast, noggin, a known specific BMP antagonist, had no effect on activin A but strongly neutralized BMP-2 and BMP-4 actions. BMP-6 action was only slightly reduced by both follistatin and noggin. Our data led to the conclusion that follistatin would not represent a determinant physiological modulator of the biological effect of BMP factors on granulosa cells.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"419-25"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25213968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

E. coli proteolytic activity in milk and casein breakdown. 大肠杆菌在牛奶中的蛋白水解活性和酪蛋白分解。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005033

Kahina Haddadi, Fatima Moussaoui, Imen Hebia, François Laurent, Yves Le Roux

{"title":"E. coli proteolytic activity in milk and casein breakdown.","authors":"Kahina Haddadi, Fatima Moussaoui, Imen Hebia, François Laurent, Yves Le Roux","doi":"10.1051/rnd:2005033","DOIUrl":"https://doi.org/10.1051/rnd:2005033","url":null,"abstract":"Previous studies have focused on both LPS and E. coli experimental mastitis and underlined the respective roles of endogenous proteolysis (including plasmin from the blood stream and other proteases from milk leukocytes), as well as the presence of E. coli in a more intricate system. The aim of this study was to assess the role of E. coli in milk proteolysis and especially that of its proteases in casein breakdown. The first part consisted in the incubation of 104 cfu.mL(-1) of the E. coli strain in raw milk at 37 degrees C for 24 h; the same milk was also incubated with 0.04% sodium azide. Several parameters were evaluated: CFU, plasmin activity, gelatinase activity and pH 4.6 insoluble peptides, including the proportion of gamma-CN. The profile of gelatinase activity was determined by zymography and identified by immunoblotting. In the second part of the study, we examined the profile of CN (alphas-, beta- and kappa-CN) breakdown by E. coli lysate. The results suggest that E. coli proteases have a direct effect on CN, and the increase of gamma-CN in inoculated milk may be generated by both plasmin and the gelatinase. Moreover, the gelatinase activity in the inoculated milk was higher after 24 h of incubation.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"485-96"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25211657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Focus on the supramolecular structure of milk fat in dairy products. 重点研究乳制品中乳脂的超分子结构。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005034

Christelle Lopez

{"title":"Focus on the supramolecular structure of milk fat in dairy products.","authors":"Christelle Lopez","doi":"10.1051/rnd:2005034","DOIUrl":"https://doi.org/10.1051/rnd:2005034","url":null,"abstract":"Bovine fat is dispersed in raw milk as natural milk fat globules, with an average diameter of 4 microm, which are enveloped in a biological membrane, the milk fat globule membrane (MFGM). However, dairy processes modify the supramolecular structure and the surface composition of milk fat. Thus, milk fat is present in many dairy products under various forms. In this study, we focused on the fact that natural milk fat globules are rarely consumed in their native state, i.e. in fresh raw milk. In most drinking milks, fat globules are homogenised in order to avoid their rising at the surface of the products. Furthermore, fat globules are heat treated to avoid the growth of micro-organisms. As a consequence of the technological process applied, the volume-weighted average diameter of fat globules in drinking milks is in the range 0.2-0.5 microm. Homogenisation of fat globules led to the partial disruption of the MFGM and to the adsorption of milk proteins. Moreover, this study showed that in cheeses, milk fat can be dispersed as (i) fat globules with the MFGM, (ii) aggregates of fat globules, (ii) homogenised fat globules, (iii) free fat and (iv) a combination of different phases and structures. The knowledge of the supramolecular structure of milk fat in dairy products is of primary importance regarding its technological, sensorial and nutritional properties.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"497-511"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25211655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 118

Mammary transmission of caprine arthritis encephalitis virus: a 3D model for in vitro study. 山羊关节炎脑炎病毒的乳腺传播:体外研究的3D模型。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005035

Christian Le Jan, Claire Bellaton, Timothy Greenland, Jean-François Mornex

{"title":"Mammary transmission of caprine arthritis encephalitis virus: a 3D model for in vitro study.","authors":"Christian Le Jan, Claire Bellaton, Timothy Greenland, Jean-François Mornex","doi":"10.1051/rnd:2005035","DOIUrl":"https://doi.org/10.1051/rnd:2005035","url":null,"abstract":"Transmission of Caprine Arthritis Encephalitis virus (CAEV) from the mother to offspring is principally mediated by infected cells from colostrum and milk. The infection of the dam is often sub-clinical, and results in increased cellularity of milk, sometimes exacerbated by bacterial co-infections. Although monocytes are the major viral host cells, several other cell types, including epithelial mammary cells, fibroblasts and endothelial cells show low levels of in vivo infection. In vitro, however, all phenotypes of mammary gland cells are individually highly sensitive to CAEV infection. This suggests that local mechanisms act to control viral expression. Our goal is to analyse the mechanisms regulating local virus infection, including the physiological status of the mammary gland and bacterial co-infections. In this work, we present the development of a model for the in vitro reconstitution of mammary gland tissue using 3D cultures in Matrigel. Mononuclear cells from the blood are added to the 3D cultures in vitro. In these experimental conditions, the mammary cells spontaneously organize into mammospheres. Blood leucocytes migrate into the culture gel, and localize particularly at the periphery of the mammospheres. Mammospheres were susceptible to infection in vitro by CAEV, as shown by a cytopathic effect and expression of late CAEV antigen p30. This model will allow the in vitro study of virus expression, transfer of infection to mammary gland cells and interactions between the mammary gland cells, infected monocytes and immunocompetent cells. It will allow the study of mechanisms participating in the control of passage of pathogens into milk, according to the physiological and CAEV-infection status of the animal, microenvironment and the presence of bacterial co-infections.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"513-23"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25213269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Dose effect of alpha-linolenic acid on lipid metabolism in the hamster. -亚麻酸对仓鼠脂质代谢的剂量效应。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005037

Anne Morise, Jacques Mourot, Michel Riottot, Pierre Weill, Evelyne Fénart, Dominique Hermier

{"title":"Dose effect of alpha-linolenic acid on lipid metabolism in the hamster.","authors":"Anne Morise, Jacques Mourot, Michel Riottot, Pierre Weill, Evelyne Fénart, Dominique Hermier","doi":"10.1051/rnd:2005037","DOIUrl":"https://doi.org/10.1051/rnd:2005037","url":null,"abstract":"In order to meet dietary requirements, the consumption of alpha-linolenic acid (ALA, 18:3 n-3) must be promoted. However, its effects on triglyceride (TG) and cholesterol metabolism are still controversial, and may be dose-dependent. The effects of increasing dietary ALA intakes (1%, 10%, 20% and 40% of total FA) were investigated in male hamsters. ALA replaced oleic acid while linoleic and saturated FA were kept constant. Triglyceridemia decreased by 45% in response to 10% dietary ALA and was not affected by higher intakes. It was associated with lower hepatic total activities of acetyl-CoA-carboxylase (up to -29%) and malic enzyme (up to -42%), which were negatively correlated to ALA intake (r(2) = 0.33 and r(2) = 0.38, respectively). Adipose tissue lipogenesis was 2-6 fold lower than in the liver and was not affected by dietary treatment. Substitution of 10% ALA for oleic acid increased cholesterolemia by 15% but, as in TG, higher ALA intakes did not amplify the response. The highest ALA intake (40%) dramatically modified the hepatobiliary metabolism of sterols: cholesterol content fell by 45% in the liver and increased by 28% in the faeces. Besides, faecal bile acids decreased by 61%, and contained more hydrophobic and less secondary bile acids. Thus, replacing 10% oleic acid by ALA is sufficient to exert a beneficial hypotriglyceridemic effect, which may be counteracted by the slight increase in cholesterolemia. Higher intakes did not modify these parameters, but a very high dose resulted in adverse effects on sterol metabolism.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"405-18"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25213967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

In vitro metabolism of rumenic acid in bovine liver slices. 牛肝片瘤胃酸体外代谢研究。

Reproduction, nutrition, development Pub Date : 2005-07-01 DOI: 10.1051/rnd:2005039

Anne De La Torre, Dominique Gruffat, Jean-Michel Chardigny, Jean-Louis Sebedio, Denys Durand, Olivier Loreau, Dominique Bauchart

{"title":"In vitro metabolism of rumenic acid in bovine liver slices.","authors":"Anne De La Torre, Dominique Gruffat, Jean-Michel Chardigny, Jean-Louis Sebedio, Denys Durand, Olivier Loreau, Dominique Bauchart","doi":"10.1051/rnd:2005039","DOIUrl":"https://doi.org/10.1051/rnd:2005039","url":null,"abstract":"Ruminant products are the major source of CLA for humans. However, during periods of fat mobilization, the liver might play an important role in CLA metabolism which would limit the availability of the latter for muscles and milk. In this context, rumenic acid (cis-9, trans-11 CLA) metabolism in the bovine liver (n = 5) was compared to that of oleic acid (n = 3) by using the in vitro liver slice method. Liver slices were incubated for 17 h in a medium containing 0.75 mM of FA mixture and 55 microM of either [1-(14)C] rumenic acid or [1-(14)C] oleic acid at 37 degrees C under an atmosphere of 95% O(2)-5% CO(2). Rumenic acid uptake by liver slices was twice (P = 0.009) that of oleic acid. Hepatic oxidation of both FA (> 50% of incorporated FA) led essentially to the production of acid-soluble products and to a lower extent to CO(2) production. Rumenic acid was partly converted (> 12% of incorporated rumenic acid) into conjugated C18:3. CLA and its conjugated derivatives were mainly esterified into polar lipids (71.7%), whereas oleic acid was preferentially esterified into neutral lipids (59.8%). Rumenic acid secretion as part of VLDL particles was very low and was one-fourth lower than that of oleic acid. In conclusion, rumenic acid was highly metabolized by bovine hepatocytes, especially by the oxidation pathway and by its conversion into conjugated C18:3 for which the biological properties need to be elucidated.","PeriodicalId":21133,"journal":{"name":"Reproduction, nutrition, development","volume":"45 4","pages":"441-51"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1051/rnd:2005039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25213970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6