Regulatory Mechanisms in Biosystems最新文献

{"title":"Peculiarities of the development of reproductive organs of ornamental crabapples (Malus spp.) depending on drought resistance","authors":"A. Konopelko, O. Opalko, O. A. Balabak, A. I. Opalko","doi":"10.15421/022344","DOIUrl":"https://doi.org/10.15421/022344","url":null,"abstract":"The current task for both pomiculture and ornamental horticulture is the formation of plant cultivars resistant to different environmental stress and maintaining their high productivity. Drought is one of the most significant environmental factors that inhibit plant growth and development and cause a set of morphological, physiological, and biochemical changes in plant organisms. The aim of our research was to discover the relationship between the development of the reproductive organs of ornamental crabapples and the physiological indicators determined by drought stress factors. The four species and three cultivars of the ornamental crabapples (Malus spp.) from the collection of the National Dendrological Park “Sofiyivka” of NAS of Ukraine were involved in the study: M. × floribunda Siebold ex Van Houtte, M. halliana Koehne, M. niedzwetzkyana Dieck, M. × purpurea (A. Barbier) Rehder, M. × purpurea ‘Ola’, M. × purpurea ‘Royalty’, and M. × purpurea ‘Selkirk’. Drought resistance was determined by the following quantitative indicators: leaf water content, relative turgidity, leaf water deficit, and excised leaf water retention capacity. The leaf water content ranged from 60.0% (M. × floribunda) to 67.2% (M. × purpurea), the relative turgidity ranged from 81.7% (M. × purpurea ‘Ola’) to 98.9% (M. halliana), and the leaf water deficit ranged from 6.8% (M. halliana) to 24.4% (of M. niedzwetzkyana). As a result, the leaves of M. halliana lost the smallest amount of water in 24 hours (50.2%), whereas M. × purpurea 'Ola' leaves lost the largest amount (77.4%). The development of the reproductive organs was estimated based on the fertility and viability of pollen grains, percentage of fruit and seed set. Pollen fertility ranged from 79.8% (of M. niedzwetzkyana) to 95.0% (M. × purpurea 'Ola'); its viability varied from 5.1% (M. niedzwetzkyana) to 51.5% (M. × floribunda). The highest fruit set percentages were recorded in M. floribunda (80.5%), while the lowest was in M. × purpurea 'Royalty' (16.4%). The average percentage of seed sets was 48.5%. The development of the reproductive organs of Malus specimens depended on their drought resistance. The fruit and seed set percentages had a strong negative correlation with the water deficit in leaves determined during the period of drought stress. The percentage of fruit set was also strongly correlated with relative turgidity. The leaf water deficit and relative turgidity were the most important physiological indicators in the drought resistance study, taking into account the need to maintain crop yield and the decorative effect of Malus plants.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49151412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age features of the microscopic structure of the intestines of the budgerigar (Melopsittacus undulatus)","authors":"М. М. Kushch, I. Y. Lavrova, І. А. Fesenko, О. S. Miroshnikova, L. М. Liakhovich, L. Kushch, О. V. Byrka","doi":"10.15421/022345","DOIUrl":"https://doi.org/10.15421/022345","url":null,"abstract":"Information about the morphology of the budgerigar intestines is a necessary condition for the preparation of diets for feeding, prevention and treatment of diseases of the digestive system, the state of which determines the intensity of growth and development of the bird’s body. The features of the histological structure of the intestines of the budgerigar (Melopsittacus undulatus) of 9 ages: 1, 3, 7, 14, 21, 30, 60, 180 and 365 days of age were studied. Histological preparations were made according to the classical method with hematoxylin and eosin staining of sections, according to Mallory and Grimelius. Morphometric parameters of microstructures were determined on preparations from a transverse section of the middle section of the duodenum, jejunum, ileum and rectum. In the intestinal wall of the budgerigar, three membranes were identified: mucous, muscular and serous. Three layers were found in the mucosa: epithelial, lamina propria, and muscular lamina. The predominant type of epitheliocytes were rim enterocytes, goblet cells were found in a smaller number, the number of which increased towards the rectum, as well as enteroendocrine cells, which were located mainly in the crypts. The muscularis mucosa consists of several layers of cells of smooth muscle tissue, which delimits the lamina propria from the muscle membrane, encircles the crypts, and accompanies the blood capillaries of the villi. The submucosa was not found. The muscular coat consists of two layers formed by smooth muscle tissue: the inner circular and the outer longitudinal. The enteric nervous system is represented by two nerve plexuses: the submucosal, located between the muscularis mucosa, and the myenteric, located in the outer layer of the muscular membrane. There was a general pattern of increase in changes in most parameters with age : the diameter of the intestine, wall thickness, mucous membrane and muscle membrane, height, width and surface area of the villi, the depth of the crypts. Changes in the size of the microstructures of the intestines were asynchronous. The most intensive changes in the size of microstructures occurred in the first month after hatching, especially in the first week. They corresponded to the values of an adult bird mainly at 21–30 days of age. A decrease in the thickness of the wall, mucosa, height of the villi, and their surface area in the direction from the duodenum to the rectum was established.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47003218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of allogeneic blood transfusion on the immunity parameters in recipient rabbits","authors":"M. O. Malyuk, O. V. Yehorov, M. A. Kulida","doi":"10.15421/022343","DOIUrl":"https://doi.org/10.15421/022343","url":null,"abstract":"Blood transfusion is always associated with immunological risks. In animals, immune conflicts can occur, because other than erythrocytes and plasma factors, many other antigens (leukocytes, trombocytes) are not accounted for. This publication is focused on morphological changes in blood of recipient rabbits following allogeneic transfusion. Modeling of blood transfusion was performed on five clinically healthy rabbits by intravenous injection of allogeneic whole blood in estimation of 5.5 mL/kg of body weight. The materials for the study were the blood samples, gathered on the 3rd, 7th, and 23th days after the transfusion. We determined that in the organism of the recipient animals, there occurred post-transfusion leukocytosis. Increase in the number of leukocytes in the experimental animals did not exceed the physiological parameters. After whole-blood transfusion, the amount of lymphocytes and granulocytes in the recipient rabbits did not significantly change over 23 days of the experiment. We recorded significant decrease in the amount of monocytes on the 3–23rd day after allogeneic transfusion in the organism of the recipient rabbits. Following the blood transfusion, percentage of T-lymphocytes in the recipient rabbits increased compared with the initial condition on the 3rd and 7th days of the experimental studies, and decreased on the 23rd day. Percentage of B-lymphocytes in the recipient rabbits after the allogeneic blood transfusion increased compared with the initial level, indicating activation of specific immunity. The whole-blood transfusion led to decrease in the percentage of O-lymphocytes in the recipient on the 3rd day of the experiment, and increase on the 7th and 23rd days of the experiment, as compared with the initial condition. The conducted studies of allogeneic transplantation of whole blood demonstrate that whole-blood transfusion entails a cascade of complex immunological reactions in the recipient animals. The prospects for further research are studies of influence of allogeneic transfusion of blood components on the parameters of specific and non-specific links of immunity of the recipient animals.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46301981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts on gut microbiota of rats with high-fat diet supplemented by herbs of Melissa officinalis, Lavandula angustifolia and Salvia officinalis","authors":"M. Bilan, M. Lieshchova, V. Brygadyrenko","doi":"10.15421/022323","DOIUrl":"https://doi.org/10.15421/022323","url":null,"abstract":"Medicinal plants are used in traditional, folk medicine and veterinary practice to treat and prevent exacerbations of chronic diseases, diseases of the cardiovascular and nervous systems, respiratory and digestive organs, liver, bile ducts, kidneys, urinary tract, to regulate metabolism and to boost immunity, etc. The therapeutic effect on the body is exerted by biologically active substances (alkaloids, glycosides, tannins, essential oils, and others) that are present in various parts of plants. Understanding the issue of interaction between the intestinal microbiota and drugs based on medicinal plants will contribute to the development of treatment protocols for various diseases, including chronic ones, by the normalization of impaired functions due to plant origin substances. In this study, we determined the effect of Melissa officinalis, Lavandula angustifolia, Salvia officinalis on the intestinal microbiota of white rats fed a high-fat diet. The addition of 5% crushed young dry shoots of S. officinalis, L. angustifolia, M. officinalis to the high-fat diet of laboratory rats significantly changed the quantitative ratio of Escherichia coli with normal and altered enzymatic properties in the intestinal content. The number of typical E. coli in rats fed with S. officinalis and L. angustifolia decreased by 1.7 and 1.6 times; non-lactose fermenting form of E. coli, on the contrary, increased by 1.8–2.1 times in rats fed with any of the medicinal plants compared to the control. Amid the addition of medicinal plants to the diet, it was not possible to isolate opportunistic enterobacteria of the genus Citrobacter, however, an increase in the number of the genus Candida fungi was observed.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43321947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of autological growth factors on activation of regenerative processes of the superficial digital flexor tendon of horses","authors":"M. O. Malyuk, E. Brusko, Y. O. Hnapovska, V. V. Tkachenko, D. V. Tarnavskiy, Т. A. Tkachenko","doi":"10.15421/022342","DOIUrl":"https://doi.org/10.15421/022342","url":null,"abstract":"Injury of the superficial digital flexor tendon is known for slow and functionally incomplete recovery, which is a problem in the equestrian horse farming. In the clinical studies, we used platelet-rich plasma, obtained from horse blood, and a specially designed scheme of rehabilitation after physical loads for the treatment of injuries of the superficial digital flexor tendon of horses. We analyzed four clinical cases of injuries of the superficial digital flexor of horses, of which 3 cases were treated using platelet-rich plasma, injected under ultrasound-diagnostic control, and one case that was treated without its administration. Ultrasound studies of the superficial digital flexor tendon revealed the following changes: increase in the volume of the tendon, damage to the fiber structure, and absence of fibrosity on the longitudinal images, and hypo-echogenic or anechogenic structure on transverse and longitudinal images. The results of the platelet-rich-plasma treatment of the horses were compared with the control (without utilization of platelet-rich plasma). As seen on the ultrasound images during the intermediate-control stage (2.5–4.0 months after injury), the horses that had received injection of platelet-rich plasma and had undergone the complex of physical exercises were forming new fibers of the superficial digital flexor tendon, which had distinct structure and alignment. This indicated the process of their physiological development. Four and a half months after being injured, the horse that had only received a symptomatic treatment combined with a complex of rehabilitating physical loads had poorly structured fibers in the damaged area, with their disordered alignment, as revealed by the longitudinal images. This suggested the formation of a scar. Transverse images showed the anechogenic areas of the injury suffered by this horse. The method of preparing and administration of platelet-rich plasma is relatively cheap, given the equipment and a qualification of a veterinary doctor. Taking into account its identified efficacy for recovery of the superficial digital flexor tendon, it would be promising to conduct further clinical trials using platelet-rich plasma for treating other injured tendons of horses, which can notably increase the quality of recovered connective tissues.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49174059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prognostic significance of the activities of matrix metalloproteinases-2 and -9 in dogs for mammary gland neoplasia (pilot study)","authors":"M. S. Kovalenko, D. D. Bilyi, K. R. Hrebeniuk","doi":"10.15421/022340","DOIUrl":"https://doi.org/10.15421/022340","url":null,"abstract":"The aim of this study was to determine the activity of matrix metalloproteinase-2 and -9 in blood and tumour tissue of female dogs with benign and malignant mammary gland neoplasias. The increased activity of matrix metalloproteinase-2 and -9 was registered in 3.8–8.9% of canines with benign neoplasias (adenoma, mixed tumour) and in 32.5–63.5% of those with malignant neoplasias (carcinoma: mixed type, ductal, tubular, tubulopapillary). Neoplastic transformation is accompanied by blood level increase of both latent and active enzyme forms. The dogs with malignant mammary gland neoplasias were diagnosed with credible increase of matrix metalloproteinases-2 activity up to 1.59–1.96 in blood plasma and up to 21.57–24.84 in tumour tissue and the increase of matrix metalloproteinases-9 activity up to 2.16–2.67 and 29.53–35.26 respectively. For benign mammary neoplasms, the proportion of dogs with enhanced expression of matrix metalloproteinase-2 was higher than the number of patients in whom an increase in the level of matrix metalloproteinase-9 or both of these enzymes was registered by 1.7 times, for dogs with malignant tumours – in blood plasma – by 1.4–1.6 times, dogs with neoplastic changed functional tissue – 1.7 and 1.9 times, respectively. Histological type and metastatic foci presence did not correlate with enzymes’ activity. The enzymes’ activity figures in benign neoplasias fluctuated within those in clinically healthy animals. A positive characteristic of determining the plasma and tissue expression level of matrix metalloproteinases in dogs with mammary tumours is the low degree of invasiveness of the method against the background of the high informativeness of the results obtained in the preoperative period. The obtained results prove the possibility of using matrix metalloproteinases-2 and -9 to predict the course and to control the treatment of mammary neoplasia.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43496590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological characteristics of the duodenum of piglets fed with various feed additives","authors":"Т. Prudyus","doi":"10.15421/022339","DOIUrl":"https://doi.org/10.15421/022339","url":null,"abstract":"Early weaning of piglets from the sow and a sharp transition to granular feeding usually leads to the development of feed stress. As a consequence, changes occur in the small intestine epithelium, which are associated with desquamation of the epithelium of the villi, crypt hyperplasia and atrophy of the villi, colonization of pathogenic microflora, which leads to a decrease in digestion and absorption of nutrients. Hence the aim of this study was to evaluate the effect of various feed additives on the morphofunctional state of the duodenal mucosa of piglets in the pre-weaning period. Forty piglets of the same age were involved in the experiment, which were divided into four groups of 10 head each. The first group of piglets was a control one and received a standard diet and free access to water from the 7th day of life. Additionally to their standard diet, the piglets of group II were given a drug which included a combination of live yeast of the genus Saccharomyces and a complex of 6 enzymes (proteases, cellulases, xylanases, α-amylases, β-glucanases and phytases) in the amount of 0.5 kg/t of feed. Piglets of group III received 0.2 kg/t of a feed additive consisting of a mixture of essential oils from plant extracts. Piglets of group IV were given a supplement with dry yeast and egg powder enriched with immunoglobulins. Piglets had constant access to feed and water. On the 14th and 28th day of the experiment, three piglets from each group were euthanized in compliance with ethical standards for animals used in experiments. According to the results of histological studies, it was established that the duodenum mucous membrane in piglets of all experimental groups was characterized by insignificant variability. Mostly, the villi had a leaf-like or finger-like shape. The crypts were mostly closely adjacent to each other. The muscle layer was well developed, represented by smooth muscles that diverged in the places where the excretory ducts of Brunner's glands pass. The lamina of the mucous membrane was represented by a loose connective tissue made of collagen fibers and, to a lesser extent, elastic fibers and small capillaries between which lymphoid cellular elements were located. The enterocytes of the apical surface of the villi had a well-defined brush border and elongated nuclei that occupied a third of the cytoplasm. Morphometric studies showed that, compared to the control group of piglets, the addition of feed additives with a mixture of essential oils increased the taste and smell of the feed, contributing to its better consumption, thus having a positive effect on the condition of the mucous membrane. On the 14th and 28th day, a significant increase in the height and width of the villi was noted. In piglets that received feed supplements with a combination of live yeast, positive dynamics were also observed in terms of increasing the ratio of villi height to crypt depth. Summarizing the experiment results, we can note that various feed addi","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43634887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dry extract from Saccharomyces cerevisiae culture with selenium-containing amino acids on the productivity and chemical composition of meat of broiler chickens","authors":"J. M. Poberezhets, R. Chudak, O. Razanova, O. Skoromna, T. Farionik, G. M. Ohorodnichuk, T. L. Holubenko, V. А. Glavatchuk","doi":"10.15421/022324","DOIUrl":"https://doi.org/10.15421/022324","url":null,"abstract":"Introduction of biologically active supplements to the diet of poultry results in maximal use of nutrients and positively influences the live weight and slaughter parameters. This promotes rational and economical use of fodders and boosts the poultry productivity. We studied a mineral feed supplement – an organic selenium compound, which has a much greater bioavailability than inorganic sources of selenium. Selenomethionine is easily digested in the organism and effectively used for synthesis of proteins. Broiler chickens of the experimental group that had been consuming the selenoamino-acid feed additive had greater live weight at the age of 14–42 days, compared with the control. Intake of the mineral supplement by the broiler chickens in the experimental group increased their mean daily increment by 8.5% and the absolute increment by 8.6%, compared with the control peers. In the experimental group of broilers, the mineral supplement increased the pre-slaughter live weight by 8.4%, weight of intact carcass by 10.1%. Also, the weight of the semi-processed carcasses (i.e. those with removed intestines and cloaca, filled crop, oviduct and ovary (in female specimens) increased by 4.3%, and the weight of the processed carcasses (the internal organs and abdominal-cavity fat and esophagus of which were removed, without head, legs and wing metacarpals) increased by 8.0%, compared with the control group of broilers. In the experimental group, the feed supplement gave 13.7% rise in the weight of the breast muscles and 14.5% increase in the weight of the thigh muscles, compared with the control group. Intake of this supplement in the experimental group promoted 7.5% increase in the weight of the gizzard by and 9.2% increase in the weight of the duodenum, as compared with the control. Usage of the selenoamino-acid feed supplement increased the protein concentration in the breast muscles of the broiler chickens by 1.9%, compared with the control. Intake of the mineral supplement increased the amounts of dry matter and fat in the thigh muscles of the experimental group, compared with the control group. Therefore, adding dry yeast extract to diet of broilers together with selenium-containing amino acids is a promising method of increasing the effectiveness of poultry farming.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46073006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative modification of proteins and antioxidant status in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes","authors":"B. Verveha, B. V. Gutyj, S. H. Lishchuk, M. I. Holubiev, R. Mylostyvyi","doi":"10.15421/022338","DOIUrl":"https://doi.org/10.15421/022338","url":null,"abstract":"Modern aspects of the pathogenesis of acute inflammation of the peritoneum that is concurrent with diabetes involves analysis of metabolic mechanisms, in particular peroxidaton of proteins – antioxidant defense. Therefore, the objective of our study was to examine the interrelation between the processes of free-radical oxidation of proteins and antioxidant system in the dynamics of development of acute generalized peritonitis against the background of streptozotocin-induced diabetes. The study was performed on 56 non-linear white mature male rats. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin (60 mg/kg). On the 14th day of the development of streptozotocin-induced diabetes, we injected 10% filtrated faeces suspension (0.5 mL) into the abdominal cavity, thus initiating acute generalized peritonitis. Oxidative modification of proteins in blood serum was studied using the I. F. Meshchyshen’s method and the condition of antioxidant protection was monitored according to the activities of superoxide dismutase, catalase, content of reduced glutathione, and the level of ceruloplasmin. The study of the parameters of free-radical oxidation of proteins and study of the condition of antioxidant system in blood of the rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes demonstrated that oxidative protein modification grew, while the parameters of the activity of antioxidant system were being inhibited, depending on the stage of acute inflammation of the peritoneum. We determined inverse correlation relationships between the products of free-radical oxidation of proteins and parameters of antioxidant system on the third and on the seventh days of modelling of combined pathology. Manifestation of acute generalized peritonitis concurring with streptozotocin-induced diabetes was accompanied by a gradual accumulation of the products of free-radical oxidation of proteins and exhaustion of the antioxidant defense during all stages of the development of acute inflammation of the peritoneum, peaking on the seventh day after administration of faecal suspension (terminal stage of peritonitis). The observed inverse correlations between the levels of oxidative modification of proteins and the activity of superoxide dismutase, catalase, reduced glutathione, and ceruloplasmin on the third and on the seventh days of modelling of combined pathology indicate a predictive role of the processes of free-radical oxidation of proteins in exhaustion of antioxidant-defense resources.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43636313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Productive parameters of rabbits fed with additives containing lactic and succinic acid, amino acid and vitamins","authors":"G. M. Ohorodnichuk, O. B. Tsyganchuk, T. L. Holubenko, O. Skoromna, O. A. Pikula, A. M. Solomon","doi":"10.15421/022333","DOIUrl":"https://doi.org/10.15421/022333","url":null,"abstract":"Prebiotics are an effective technological element of increasing the productivity of animal husbandry. Currently, prebiotics are being broadly used in cuniculture. Those drugs are a promising means of stimulating growth and development of animals. Prebiotics optimize the metabolic processes and increase the level of nutrient metabolism. Our studies revealed the effect of a prebiotic drug, which contains lactic and succinic acids, aminoacids, vitamins А, В1, В2, В3, Е, on the growth intensity of rabbits. The studies were performed on the young 34 to 160-days-old rabbits of the Hyplus meat hybrid. Animals of the control group were fed ad libitum with full-diet granulated mixed feeds and had free access to water. Animals of the experimental groups were fed feeds of the control-group diet, supplemented by prebiotic additive Prebiolakt-Kr in the doses of 1.5, 2.0, and 2.5 g/kg of the live weight. Every 14 days during the feeding period, the growth intensity of the rabbits was controlled according to individual live weight and average daily increment. Addition of Prebiolakt-Kr to the rabbits’ diet increased the live weight of animals by the end of feeding. We verified and confirmed the positive effect of the prebiotic on the intensity of growth and development of the growing rabbits. According to the absolute increment, the advantage over the rabbits in the control accounted for 7.9–11.9%. Feeding the supplement increased the meat volumes of the rabbits. Also, Prebiolakt-Kr added to the diet caused higher chest girth/diagonal body length index. The animals that were receiving the prebiotic feed supplement used the diet more effectively and had better metabolism of nutrients. The studies revealed a dose-dependent effect of prebiotic on the growth intensity of the rabbits. At the same time, the best stimulating action was displayed by supplementing the diet of the young rabbits by 2.0 and 2.5 g/ind. of Prebiolakt-Kr. The conducted studies have confirmed the benefits of using prebiotic drugs in rabbit husbandry, which improve metabolism and increase body-weight gain.","PeriodicalId":21094,"journal":{"name":"Regulatory Mechanisms in Biosystems","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44741658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}