Research in Biotechnology最新文献_第2页

PRODUCTION OF INDUSTRIALLY IMPORTANT ENZYMES BY THERMOBACILLI ISOLATED FROM HOT SPRINGS OF INDIA 从印度温泉中分离的热杆菌生产工业上重要的酶

Research in Biotechnology Pub Date : 2017-03-31 DOI: 10.25081/RIB.2017.V8.3594

A. Dhyani, Ritu Gururani, S. Selim, P. Adhikari, Avinash Sharma, V. Pande, A. Pandey

{"title":"PRODUCTION OF INDUSTRIALLY IMPORTANT ENZYMES BY THERMOBACILLI ISOLATED FROM HOT SPRINGS OF INDIA","authors":"A. Dhyani, Ritu Gururani, S. Selim, P. Adhikari, Avinash Sharma, V. Pande, A. Pandey","doi":"10.25081/RIB.2017.V8.3594","DOIUrl":"https://doi.org/10.25081/RIB.2017.V8.3594","url":null,"abstract":"Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"15 1","pages":"19-28"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82430894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protein difference among the leaf explants determined for shoot regeneration and callus growth in Mulberry (Morus indica L.) 桑树叶片外植体对芽再生和愈伤组织生长的蛋白质差异研究

Research in Biotechnology Pub Date : 2017-03-31 DOI: 10.19071/RIB.2017.V8.3201

D. Chitra, B. Chinthapalli, G. Padmaja

引用次数: 1

Serum biochemical properties and liver morphology of broiler chicken as affected by feeding Misai kucing (Orthosiphon stamineus) as a supplementary diet 饲粮补充Misai kucing对肉鸡血清生化特性和肝脏形态的影响

Research in Biotechnology Pub Date : 2016-12-19 DOI: 10.19071/RIB.2016.V7.3093

M. Malahubban, Zakry Fitri Ab Aziz

{"title":"Serum biochemical properties and liver morphology of broiler chicken as affected by feeding Misai kucing (Orthosiphon stamineus) as a supplementary diet","authors":"M. Malahubban, Zakry Fitri Ab Aziz","doi":"10.19071/RIB.2016.V7.3093","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.3093","url":null,"abstract":"To evaluate the effects of ground leaf of Misai kucing ( Orthosiphon stamineus ) as a dietary supplement on serum biochemical parameters and liver morphology. One hundred and sixty one-day old male broiler chickens (n=160) were distributed into four treatment groups, with five replicates of eight birds in each group: the control group (diet without additives); the group dietary treatments, Diet OS2 (Basal diet + 2g/kg O. stamineus ); Diet OS4 (Basal diet + 4g/kg O. stamineus ) and Diet OS8 (Basal diet + 8g/kg O. stamineus ). After 42 days, 40 birds were randomly selected for serum biochemical profile analysis involving pancreatic, renal, and hepatic functions (urea, sodium, potassium, chlorine, aspartate transaminase (AST), alkaline transaminase (ALT), alkaline phosphatase (ALP), glucose, cholesterol, triglycerides, total protein, albumin, and globulins). Present study found that serum levels of cholesterol, triglycerides, urea, AST, ALT and ALP were significantly lower suggesting that the O. stamineus ground leaf possibly do not cause kidney and liver impairment, mainly, at the higher dosage (8g/kg). Present study concluded that the broiler chicken fed O. stamineus ground leaf at a rate 8 g/kg was the most promising dietary supplement to enhance health without deleterious effects on serum biochemical properties and morphological components of liver. In addition, it reduces abdominal fats and serum cholesterol. This study has provide evident that medicinal plant, O. stamineus can potentially substituted the use of additive synthetic.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80568712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effects of the bendazol fungicide on in vitro development of the nim (Azadirachta indica A. JUSS) 苯达唑杀菌剂对印楝离体发育的影响

Research in Biotechnology Pub Date : 2016-10-28 DOI: 10.19071/RIB.2016.V7.3078

E. Santos, Robson Antonio de Souza, M. J. Medeiros, G. D. Alves, L. Houllou

{"title":"Effects of the bendazol fungicide on in vitro development of the nim (Azadirachta indica A. JUSS)","authors":"E. Santos, Robson Antonio de Souza, M. J. Medeiros, G. D. Alves, L. Houllou","doi":"10.19071/RIB.2016.V7.3078","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.3078","url":null,"abstract":"Two experiments were conducted to evaluate Bendazol fungicidal effects in neem micropropagation. In these experiments, the nodal segment explants from in vitro plants were used. In the first experiment, the explants remained in DKW culture medium for a period of 30 days containing different concentrations of Bendazol (M1 -50, M2 - 100, M3 - 200, and M4 - 400 mg.L -1 ). The control treatment (M0) was prepared with DKW medium + BAP (0.225 mg.L -1 ). In the second experiment, the explants were maintained for only one week in media supplemented with Bendazol or BAP, and then they were transferred and kept in free Bendazol/BAP media for three weeks. In each experiment, the design was completely randomized with five treatments, 10 replicates per treatment, and one explant/cultivation flask. The variables analyzed included the formation of calluses and roots, lateral bud development, shoot height, contamination and plant death. There was no significant difference in tree variables (shoot, callus formation and shoot height) between treatments in both experiments. There was no death, plant contamination and rooting during the experiment. The results indicate that Bendazol can be used at low doses for in vitro neem cloning thereby replacing BAP and ultimately reducing production costs.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73695518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation of micro-algae for Production of Biodiesel: An optimized Process 微藻生产生物柴油的优化工艺研究

Research in Biotechnology Pub Date : 2016-08-28 DOI: 10.19071/rib.2016.v7.3037

A. Demissie, B. Chinthapalli, Shumet Tenaw, D. Chitra

{"title":"Cultivation of micro-algae for Production of Biodiesel: An optimized Process","authors":"A. Demissie, B. Chinthapalli, Shumet Tenaw, D. Chitra","doi":"10.19071/rib.2016.v7.3037","DOIUrl":"https://doi.org/10.19071/rib.2016.v7.3037","url":null,"abstract":"Microalgae are considered as one of the potential source of biodiesel for the future. The search to obtain the potential strains from the algal diversity capable of producing oil is critical for sustainable production of biodiesel. In the present study, microalgae biomass with oil/lipid accumulation capability and their morphological features was isolated from Lake Abaya and Chamo. The algal biomass was cultivated in vitro and media optimization for maximum biomass was done using different basal media, BG-11 medium, and Chu -10. In addition the various carbon sources, nitrogen sources, pH and temperature were considered in this study for optimization. Green algae Oedogonium , Chlorella and Cladophora species were observed to be dominant species and the maximum oil per dry algal biomass was found to be from Oedogonium sp. Thus from the present study for the cultivation of the selected algae, BG-11 medium supplemented with tryptone (0.2%) sucrose (2%) and pH- 6 with incubation temperature of 30 0 C was found to be suitable. These results suggest that Oedogonium sp. has several desirable features that make it a potential candidate for biodiesel production.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90757152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Control of contaminants during introduction and establishment of Bambusa vulgaris in vitro 竹笋引种建立过程中污染物的控制

Research in Biotechnology Pub Date : 2016-08-08 DOI: 10.19071/rib.2016.v7.3056

G. Torres, L. Houllou, Robson Antonio de Souza

{"title":"Control of contaminants during introduction and establishment of Bambusa vulgaris in vitro","authors":"G. Torres, L. Houllou, Robson Antonio de Souza","doi":"10.19071/rib.2016.v7.3056","DOIUrl":"https://doi.org/10.19071/rib.2016.v7.3056","url":null,"abstract":"The aim of this work was to test techniques to reduce microbial contamination in the phases of introduction and establishment of the in vitro cultivation of Bambusa vulgaris through two experiments. The first experiment was carried out in a completely randomized design using a factorial arrangement (pre-treatment of nodal segments using or not a solution of Derosal 500 SC ® and Chloramphenicol × culture medium with half or full concentration of salts × culture medium with presence or absence of sucrose × culture medium with presence or absence of Plant Preservative Mixture TM ). In a second experiment, carried out in a completely randomized design, the effect of different fungicides associated to Chloramphenicol in a liquid culture medium was tested. It was possible to verify that the isolated effects of the pre-treatment by immersion of the nodal segments in a solution of 4 mL L -1 of Derosal 500 SC ® and 200 mg L -1 of Chloramphenicol for 30 minutes and explants placed in a sucrose-free medium reduced fungal contamination. In the second experiment, the treatment that reduced fungal contamination corresponded to explants placed for seven days in a liquid medium with half the concentration of salts, sucrose-free, with 2 mL L -1 of Plant Preservative Mixture TM and with 4 mL L -1 of Derosal 500 SC ® and 200 mg L -1 of Chloramphenicol.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84009385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut 椰芽分生组织三种不同方法诱导胚性愈伤组织的比较研究

Research in Biotechnology Pub Date : 2016-04-25 DOI: 10.19071/RIB.2016.V7.2953

U. Bhavyashree, K. LakshmiJayaraj, T. P. Fayas, K. S. Muralikrishna, K. K. Sajini, M. K. Rajesh, A. Karun

{"title":"A comparative study of three different methods of shoot meristem excision for induction of embryogenic calli in coconut","authors":"U. Bhavyashree, K. LakshmiJayaraj, T. P. Fayas, K. S. Muralikrishna, K. K. Sajini, M. K. Rajesh, A. Karun","doi":"10.19071/RIB.2016.V7.2953","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2953","url":null,"abstract":"A protocol was standardized to maximize yields of embryogenic calli from shoot meristem culture of coconut. Three different shoot meristem excision methods were tested viz ., excision of shoot meristem aseptically from in vitro germinated embryo after 10-12 days, excision of shoot meristem from in vitro germinated embryo subjected to GA3 treatment for five days and excision of shoot meristem from fresh embryo. The primary calli induction after 30 days of culture incubation for the three treatments were 21%, 27% and 79% respectively. Further, the primary calli formed from the shoot meristem excised from fresh embryo gave rise to 56% of embryogenic calli. The calli obtained from the shoot meristem which were excised from in vitro germinated embryo formed less percentage of embryogenic calli because of the presence of cotyledonary tissues which inhibited the multiplication of meristematic tissues. In the case of shoot meristem extracted from GA3-treated embryos, the percentage of non-embryogenic calli was more compared to the shoot meristem excised from fresh embryo. It was observed that the addition of GA3 in the initial stages of culture inhibited the formation of embryogenic calli and favored direct shoot development. Currently, the shoot meristem excised from fresh embryo is being employed for scaling up the planting material production from released varieties of coconut.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87961050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of seed storage protein markers for drought tolerance in mungbean 绿豆抗旱种子贮藏蛋白标记的鉴定

Research in Biotechnology Pub Date : 2016-03-23 DOI: 10.19071/RIB.2016.V7.2895

S. Tripathy, P. Mohanty, M. Jena, S. Dash, D. Lenka, D. Mishra, P. Nayak, D. Swain, R. Ranjan, K. Pradhan, N. Senapati, P. Mohapatra, G. Dash

{"title":"Identification of seed storage protein markers for drought tolerance in mungbean","authors":"S. Tripathy, P. Mohanty, M. Jena, S. Dash, D. Lenka, D. Mishra, P. Nayak, D. Swain, R. Ranjan, K. Pradhan, N. Senapati, P. Mohapatra, G. Dash","doi":"10.19071/RIB.2016.V7.2895","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2895","url":null,"abstract":"A set of 292 mungbean germplasm accessions including 62 popularly adapted local land races and two wild forms ( Vigna radiata var. sublobata), important breeding lines and standard ruling varieties were screened for drought stress tolerance at seedling stage. Eight genotypes e.g., C. No. 35, OUM 14-1, OUM 49-2, Pusa 9072, OM 99-3, Banapur local B, Nipania munga, Kalamunga 1-A) have been identified to possess drought tolerance. Globulin seed storage protein profiling was carried out in 19 selected mungbean genotypes comprising eight drought tolerant, seven drought sensitive, two wild forms of mungbean (TCR 20 and TCR 213) and two standard checks (LGG 460 and T 2-1 ) to explore differentially expressed polypeptides. Seed protein profiles revealed 15 scorable polypeptide bands with molecular weights ranging from 10.0 to 102.2kD. A specific 12.8kD polypeptide band was present in all above drought tolerant test genotypes including the wild accession TCR 20. Such a polypeptide band may serve as useful biochemical marker for identification of drought tolerant genotypes in mungbean. Key words: Genetic diversity, seed storage protein profile, wild and cultivated Vigna radiata.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"637 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77027035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A comparative study on field performance of micropropagated plants and stem cutting derived plants of S-36 cultivar of Mulberry (Morus indica L.) 桑树(Morus indica L.) S-36品种微繁苗与茎切苗田间性能比较研究

Research in Biotechnology Pub Date : 2016-02-10 DOI: 10.19071/rib.2016.v7.2930

D. Chitra, B. Chinthapalli, G. Padmaja

引用次数: 3

Impact of two disinfectants on detachment of Enterococcus faecalis from polythene in aquatic microcosm 两种消毒剂对水生微生物中粪肠球菌脱离聚乙烯的影响

Research in Biotechnology Pub Date : 2016-02-10 DOI: 10.19071/RIB.2016.V7.2981

C. L. Djimeli, A. T. Arfao, V. Rossi, N. Nsulem, V. Raspal, G. Bricheux, M. Nola, T. Sime-Ngando

{"title":"Impact of two disinfectants on detachment of Enterococcus faecalis from polythene in aquatic microcosm","authors":"C. L. Djimeli, A. T. Arfao, V. Rossi, N. Nsulem, V. Raspal, G. Bricheux, M. Nola, T. Sime-Ngando","doi":"10.19071/RIB.2016.V7.2981","DOIUrl":"https://doi.org/10.19071/RIB.2016.V7.2981","url":null,"abstract":"After cell adhesion processes in microcosm, the impact of sodium hypochlorite (NaOCl) and hydrogen peroxide (H 2 O 2 ) on the detachment of Enterococcus faecalis from polythene fragments immersed in water under stationary and dynamic conditions was assessed. The abundance of planktonic cells was also evaluated. The density of E. faecalis adhered in absence of disinfectant fluctuated between 2 and 4 units (Log CFU/cm 2 ). After living in disinfected water, the density of E. faecalis remained adhered to polythene sometimes reached 2 units (Log CFU/Cm 2 ) . This highest abundance of cells remained adhered was recorded with cells coming from the lag, exponential and stationary growth phases in water treated with 0.5‰ NaOCl. In H 2 O 2 disinfected water, the highest value was recorded at all cells growth phases with 5‰ H 2 O 2 concentration. Adhered E. faecalis cells have been sometimes completely or partially decimated respectively by NaOCl and H 2 O 2 treated water. Considering separately each experimental condition, it was noted that increasing the concentration of disinfectant caused a significant decrease (P≤0.01) in abundance of cells stay adhered after living in water disinfected by the two disinfectants. Changes in disinfectant concentrations in different experimental conditions had an impact on the detachment of E. faecalis cells from the substrates.","PeriodicalId":21082,"journal":{"name":"Research in Biotechnology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72693008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3