Research communications in molecular pathology and pharmacology最新文献_第2页

Protective effect of tamoxifen, a synthetic non-steroidal antiestrogen, on phenelzine and 1-methyl-4-phenylpyridinium ion (MPP+)-induced hydroxyl radical generation in rat striatum. 合成非甾体抗雌激素他莫昔芬对苯肼和1-甲基-4-苯基吡啶离子(MPP+)诱导的大鼠纹状体羟基自由基生成的保护作用。

Research communications in molecular pathology and pharmacology Pub Date : 2009-01-01

Toshio Obata, Masahiro Aomine

引用次数: 0

Protective effect of tamoxifen, a synthetic non-steroidal antiestrogen, on phenelzine and 1-methyl-4-phenylpyridinium ion (MPP+)-induced hydroxyl radical generation in rat striatum. 合成非甾体抗雌激素他莫昔芬对苯肼和1-甲基-4-苯基吡啶离子(MPP+)诱导的大鼠纹状体羟基自由基生成的保护作用。

Research communications in molecular pathology and pharmacology Pub Date : 2009-01-01

Toshio Obata, Masahiro Aomine

引用次数: 0

Effect of caffeine and alcohol on the toxicity and metabolism of methacrylonitrile in male Sprague-Dawley rats. 咖啡因和酒精对雄性Sprague-Dawley大鼠甲基丙烯腈毒性和代谢的影响。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Mohammed Y H Farooqui, Maria Trevino, Isabella Garcia

{"title":"Effect of caffeine and alcohol on the toxicity and metabolism of methacrylonitrile in male Sprague-Dawley rats.","authors":"Mohammed Y H Farooqui, Maria Trevino, Isabella Garcia","doi":"","DOIUrl":"","url":null,"abstract":"This study reports the toxicity and metabolism of methacrylonitrile (MeAN) in normal male Sprague-Dawley rats and those pre-treated with caffeine, alcohol or both. Rats were divided into groups often. One group received an oral dose by gavage of 6 % MeAN solution in corn oil (equivalent to 0.5 LD50). Other three groups of rats were pre-treated with alcohol (2 ml of 50% solution in water), caffeine (1 ml of 2% solution in water) or both alcohol and caffeine 12 hr before receiving MeAN dose by gavage. The rats were observed for mortality, cholinomimetic and central nervous system (CNS) effects and urinary dysfunction for 6 hr. The concentrations of cyanide, thiocyanate and glutathione (GSH) were determined in blood, liver, kidney and brain. Alcohol and alcohol + caffeine pre-treatment caused significant increase in cholinomimetic, CNS and urinary dysfunction effects of MeAN and mortality. However, caffeine alone pre-treatment protected rats from these effects. In the rats treated with MeAN alone and those pre-treated with alcohol and alcohol + caffeine the GSH concentrations significantly decreased in liver, brain and kidney. In the rats pre-treated with caffeine alone the concentrations of GSH were not significantly different from controls. In the rats treated with MeAN alone and those pretreated with alcohol and alcohol + caffeine the cyanide and thiocyanate concentrations increased in the blood and other organs up to 2-4 folds whereas in rats pre-treated with caffeine alone the concentrations of cyanide and thiocyanate were not significantly different from controls. Western Blot experiment showed CYP2E1 induction in rats pretreated with alcohol and MeAN. These results suggest that caffeine inhibited and alcohol enhanced toxicity and metabolism of MeAN.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"120-121 1-6","pages":"79-92"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29795278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of synthetic and natural products as pesticides (CSYNAP): a new class of antifungal agents. 将合成产品和天然产品结合起来作为杀虫剂（CSYNAP）：一类新型抗真菌剂。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Archna Rani, Sapna Jain, Prem Dureja, Praveen K Tripathi, Kamalendra Singh

{"title":"Combination of synthetic and natural products as pesticides (CSYNAP): a new class of antifungal agents.","authors":"Archna Rani, Sapna Jain, Prem Dureja, Praveen K Tripathi, Kamalendra Singh","doi":"","DOIUrl":"","url":null,"abstract":"In the present communication some dehydrated dialdol products such as 1, 5 - Diphenyl pent - 1, 4 - diene - 3 - one (A1); 1, 9 - Diphenylnon - 1, 3, 6, 8 - tetraene - 5 - one (A2); 1, 5 - di (2 - hydroxyphenyl) pent - 1, 4 - diene - 3 - one (A3); 1, 5 - difuran pent - 1, 4 - diene - 3 - one (A4); 1, 5 - di [4 - bis (N, Ndimethyl) phenyl] pent - 1, 4 - diene - 3 - one (A5) were screened for their antifungal activity. To reduce their adverse effect on the environment, for the first time, we have attempted to screen the antifungal activity of these synthetic compounds in conjunction with selected natural products. The natural products that were used in our study include Nicotine tobaccum and Neem oil (Azadirachta indica). A set of 15 samples was tested against highly pathogenic and of extensive host range fungi Sclerotium rolfsii, Rhizactonia bataticola, Fusarium udum. The filter paper disc assay to monitor antifungal effect revealed significant and interesting results. We found that the use of the combination of natural and synthetic pesticides is more effective and environmentally healthy compared to just synthetic chemicals and/or less available natural products. These results obtained from the combined use of natural and synthetic chemicals lead us to suggest to a new class of less toxic but more effective pesticides. We call it group as CSYNAP, i. e. Combination of SYnthetic and NAtural products as Pesticides.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"120-121 1-6","pages":"15-21"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29796522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

6,(5H)-phenanthridinone protects against carbon tetrachloride-induced cytotoxicity in human HepG2 cells. 6，(5H)-phenanthridinone对四氯化碳诱导的HepG2细胞毒性具有保护作用。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Paul C Grivas, Seigo Tanaka, Kunihiro Ueda, Giffe Johnson, Raymond D Harbison

{"title":"6,(5H)-phenanthridinone protects against carbon tetrachloride-induced cytotoxicity in human HepG2 cells.","authors":"Paul C Grivas, Seigo Tanaka, Kunihiro Ueda, Giffe Johnson, Raymond D Harbison","doi":"","DOIUrl":"","url":null,"abstract":"Carbon tetrachloride (CCl4) is a compound associated with free radical mediated hepatotoxicity in humans and laboratory animals. Previous research indicates that the cytotoxicity caused by CCl4 may be mediated by the rapid induction of PARP-1, a nuclear repair enzyme, which results in celluar depletion of NAD+ and ATP. Animal models indicate that the inhibition of PARP-1 after CCl4 exposure will attenuate cytotoxicity in mouse hepatocytes. In this investigation, the potential hepatoprotective effects of the PARP-1 inhibitor 6,(5H)-phenanthridinone against CCl4-induced hepatotoxicity was tested in human cells from the HepG2 primary hepatoma cell line. Cytotoxicity assay results indicate significant reductions in cell death with treatment of 20uM and 40uM solutions of 6,(5H)-phenanthridinone. PARP-1 activity assay results confirm that these protective effects correspond to the inhibition of PARP-1 by 6,(5H)-phenanthridinone. The findings in this study indicate that the effect of PARP-1 inhibition on cytotoxicity in human hepatocytes after CCl4 insult is consistent with the effect of PARP-1 inhibition on cytotoxicity found in animal models.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"120-121 1-6","pages":"117-26"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29795274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleomorphism of cancer cells with the expression of plectin and concept of filament bundles in human hepatocellular carcinoma. 肝癌细胞的多形性与凝集素的表达及丝束的概念。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Yi-Hsiang Liu, Chin-Chin Ho, Chiung-Chi Cheng, Ren-Jeng Pei, Yung-Hsiang Hsu, Kun-Tu Yeh, Ming-Chuang Tsai, Yih-Shyong Lai

{"title":"Pleomorphism of cancer cells with the expression of plectin and concept of filament bundles in human hepatocellular carcinoma.","authors":"Yi-Hsiang Liu, Chin-Chin Ho, Chiung-Chi Cheng, Ren-Jeng Pei, Yung-Hsiang Hsu, Kun-Tu Yeh, Ming-Chuang Tsai, Yih-Shyong Lai","doi":"","DOIUrl":"","url":null,"abstract":"Intermediate filaments are important in building the architecture of liver cells and are proposed to interact with other cellular components. Among intermediate filament associated proteins, plectin is a versatile cytoskeletal linkage protein which has been shown to interact with a variety of cytoskeletal structures. Intermediate filament and plectin might play some roles in tumorigenesis of human hepatocellular carcinoma since cells of hepatocellular carcinoma were morphologically different from normal liver. Plectin exhibited wide distribution spectrum among various tissues, however, it was poorly investigated in human liver and hepatoma tissues. In this paper, we studied the plectin expression in 18 cases of human hepatocellular carcinoma and normal hepatocytes by immunohistochemistry. The results revealed that plectin expression was deficient in human hepatocellular carcinoma and was probably through post-translational modification. Many 0.4 to 0.8 microm-thick keratin bundles were found in intermediate filament extracts of liver and hepatoma tissues. These bundles were greater in diameter about 40 to 80 times of single intermediate filament. We speculated that intermediate filament organized into \"filament bundles\" to maintain the shape of normal cells. In cancer cells, plectin was deficient and the irregularly loosened filament bundles could cause pleomorphism of cancer cells.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"120-121 1-6","pages":"43-54"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29795349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycine affects valproate hepatotoxicity. 甘氨酸影响丙戊酸肝毒性。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Lara Junius, Thomas Brune, Michael Deters, Johannes Schulze, Claus-Peter Siegers

{"title":"Glycine affects valproate hepatotoxicity.","authors":"Lara Junius, Thomas Brune, Michael Deters, Johannes Schulze, Claus-Peter Siegers","doi":"","DOIUrl":"","url":null,"abstract":"Valproate is a widely used anticonvulsant drug. Valproate is linked to hepatotoxicity which is rare but potentially lethal. The pathomechanism is not yet completely understood. Previous studies have shown a protective effect of glycine on this toxicity in rat hepatocytes (Vance et al., 1994). In the present study we investigated the hepatoxicity of 1-4 mM valproate in combination with glycine through absorption of neutral red from human hepatoma cells (Hep G2) in monolayer cell culture. Cell toxicity was measured by enzyme release with standard photometric test kits. In addition, a histomorphological evaluation was performed. A significant increase in the IC50 value of valproate cytotoxicity after addition of 12 mmol/l glycine was found. The average release of mitochondrial glutamate dehydrogenase as indicator of cell membrane damage decreased after addition of glycine. Cells treated with valproate in combination with 12 mmol/l glycine showed less histomorphological deformation of the nucleus and improved cell adherence. In conclusion a hepatoprotective effect of glycine on valproate-induced toxicity is also given in human hepatocytes. A nonspecific hepatoprotective effect of glycine can be assumed at least in vitro.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"120-121 1-6","pages":"93-104"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29795354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of positional candidates for bovine placental genes responsible for early embryonic death during cloning-attempted pregnancy. 克隆妊娠早期胚胎死亡的牛胎盘基因位置候选基因的鉴定。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Takahisa Yamada, Youji Muramatsu, Yukio Taniguchi, Yoshiyuki Sasaki

{"title":"Identification of positional candidates for bovine placental genes responsible for early embryonic death during cloning-attempted pregnancy.","authors":"Takahisa Yamada, Youji Muramatsu, Yukio Taniguchi, Yoshiyuki Sasaki","doi":"","DOIUrl":"","url":null,"abstract":"Our previous study detected 291 and 77 genes showing early embryonic death-associated elevation and reduction of expression, respectively, in the fetal placenta of the cow carrying somatic nuclear transfer-derived cloned embryo. In this study, we mapped the 10 genes showing the elevation and the 10 genes doing the reduction most significantly, using somatic cell hybrid and bovine draft genome sequence. We then compared the mapped positions for these genes with the genomic locations of bovine quantitative trait loci for still-birth and/or abortion. Among the mapped genes, peptidylglycine alpha-amidating monooxygenase (PAM), spectrin, beta, nonerythrocytic 1 (SPTBNI), and an unknown novel gene containing AU277832 expressed sequence tag were intriguing, in that the mapped positions were consistent with the genomic locations of bovine still-birth and/or abortion quantitative trait loci, and thus identified as positional candidates for bovine placental genes responsible for the early embryonic death during the pregnancy attempted by somatic nuclear transfer-derived cloning.","PeriodicalId":21045,"journal":{"name":"Research communications in molecular pathology and pharmacology","volume":"120-121 1-6","pages":"5-13"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29796521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes. PDHB、SORBS1和EDG1基因启动子区域多态性显示大理石纹相关表达变化。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Takahisa Yamada, Seiki Sasaki, Shin Sukegawa, Youji Muramatsu, Youichi Takahagi, Sachiyo Yoshioka, Tatsuo Fujita, Mitsuo Morita, Hiroshi Murakami, Fumiki Morimatsu, Yoshiyuki Sasaki

引用次数: 0

Polymorphisms for 7 expressed sequence tags showing marbling-associated expression changes. 7个表达序列标签的多态性显示大理石纹相关的表达变化。

Research communications in molecular pathology and pharmacology Pub Date : 2007-01-01

Shin Sukegawa, Takahisa Yamada, Seiki Sasaki, Youji Muramatsu, Youichi Takahagi, Sachiyo Yoshioka, Tatsuo Fujita, Mitsuo Morita, Hiroshi Murakami, Fumiki Morimatsu, Yoshiyuki Sasaki

引用次数: 0