Proceedings of Universities. Applied Chemistry and Biotechnology最新文献_第5页

Modulation of growth and chemical element accumulation in Fragaria × ananassa plants in vivo under the effect of silicon chelates 在硅螯合物的作用下调节 Fragaria × ananassa 植物的体内生长和化学元素积累

Proceedings of Universities. Applied Chemistry and Biotechnology Pub Date : 2023-12-26 DOI: 10.21285/2227-2925-2023-13-4-494-505

E. Ambros, E. S. Krupovich, Yu. P. Kolmogorov, E. Trofimova, I. S. Gusev, B. Goldenberg

{"title":"Modulation of growth and chemical element accumulation in Fragaria × ananassa plants in vivo under the effect of silicon chelates","authors":"E. Ambros, E. S. Krupovich, Yu. P. Kolmogorov, E. Trofimova, I. S. Gusev, B. Goldenberg","doi":"10.21285/2227-2925-2023-13-4-494-505","DOIUrl":"https://doi.org/10.21285/2227-2925-2023-13-4-494-505","url":null,"abstract":"Due to the protective role played by silicon in plants against unfavorable environmental conditions, siliconcontaining preparations are of considerable interest as biostimulants. In this work, a mechanical composite of rice husk and green tea containing soluble silica chelate complexes was used as the source of silicon. The study aims to examine the effect of silicon chelates on the growth and physiological parameters and the chemical composition of Fragaria × ananassa plants (Solnechnaya Polyanka variety) under greenhouse conditions. The plants were watered using water without a mechanical composite (control) or an aqueous solution containing 0.3 g/L of mechanical composite twice per period. Sampling was carried out one week after the last treatment. In order to determine the concentration of chemical elements (Si, P, S, Cl, K, Ca, Ti, Mn, Fe, Ni, Cu, Zn, Br, Rb, Sr, and Mo) in the roots and shoots of garden strawberry, it was proposed to use synchrotron X-ray fluorescence analysis. The use of the mechanical composite was shown to increase the amount of chlorophyll a, chlorophylls a and b, and carotenoids; decrease the amount of hydrogen peroxide; and increase the activity of the main antioxidant enzymes (superoxide dismutase, catalase, and peroxidase). It was determined that under the effect of the preparation, silicon accumulates primarily in shoots, affecting the accumulation of micro- and macroelements in the shoots and roots of plants. The obtained results substantiate the use of silicon-containing “green chemistry” as a means of controlling the growth and development of garden strawberry plants under in vivo conditions.","PeriodicalId":20677,"journal":{"name":"Proceedings of Universities. Applied Chemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the overexpression of external alternative NADH dehydrogenase gene in Arabidopsis on the resistance of transformed tobacco plants to negative temperatures 拟南芥外部替代 NADH 脱氢酶基因的过表达对转化烟草植物抗负温度能力的影响

Proceedings of Universities. Applied Chemistry and Biotechnology Pub Date : 2023-12-26 DOI: 10.21285/2227-2925-2023-13-4-516-522

G. B. Borovskii, E. L. Gorbyleva, A. Katyshev, N. A. Korotaeva

{"title":"Effect of the overexpression of external alternative NADH dehydrogenase gene in Arabidopsis on the resistance of transformed tobacco plants to negative temperatures","authors":"G. B. Borovskii, E. L. Gorbyleva, A. Katyshev, N. A. Korotaeva","doi":"10.21285/2227-2925-2023-13-4-516-522","DOIUrl":"https://doi.org/10.21285/2227-2925-2023-13-4-516-522","url":null,"abstract":"The study aims to confirm the functional activity and localization of the At_NDB2 transgenic protein of Arabidopsis in tobacco cells and to evaluate the effect produced by the permanent expression of external alternative NADH dehydrogenase on the resistance of a heat-loving plant to low temperatures. Proteins and mitochondria were isolated from the leaves of tobacco plants grown at 25 °С (day/night) at the 7-leaf stage. At_NDB2 protein localization in mitochondria was determined via electrophoresis and immunoblotting. The functional activity of At_NDB2 was confirmed through the polarography of isolated mitochondria and the specific mitochondrial complex I inhibitor (rotenone). It was also found that the cyanide-resistant respiration rate and the activity of an alternative oxidase enzyme were significantly higher in transgenic plants than in wild-type plants. In order to determine the resistance to low temperatures, the parent and transgenic tobacco plants were grown in soil until the 2–3 and 6–7 leaf stages, after which they were kept at 3 to -3 °С for a day in the dark and left to regrow at 25 °С for seven days. Although it was previously shown that oxidative stress is reduced in transgenic plants at low temperatures as compared to wild-type plants, it was established that the tolerance of transgenic and nontransformed plants does not differ. Thus, alternative NADH dehydrogenase activity was found to reduce oxidative stress and increase alternative oxidase activity, without enhancing the resistance of Nicotiana tabacum to negative temperatures.","PeriodicalId":20677,"journal":{"name":"Proceedings of Universities. Applied Chemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of growth medium composition on the viability and genetic stability of Chrysanthemum × morifolium Ramat. explants under in vitro cold storage conditions 生长培养基成分对离体冷藏条件下菊花 × morifolium Ramat.外植体活力和遗传稳定性的影响

Proceedings of Universities. Applied Chemistry and Biotechnology Pub Date : 2023-12-26 DOI: 10.21285/2227-2925-2023-13-4-483-493

N. N. Ivanova, V. A. Tsiupka, N. V. Korzina

{"title":"Effect of growth medium composition on the viability and genetic stability of Chrysanthemum × morifolium Ramat. explants under in vitro cold storage conditions","authors":"N. N. Ivanova, V. A. Tsiupka, N. V. Korzina","doi":"10.21285/2227-2925-2023-13-4-483-493","DOIUrl":"https://doi.org/10.21285/2227-2925-2023-13-4-483-493","url":null,"abstract":"Cultivation of plants at low positive temperatures is promising for the in vitro preservation of valuable Chrysanthemum × morifolium Ramat. varieties. The study aims to examine the effect of chlorocholine chloride concentrations in a specialized medium on the viability and the physiological and morpho-anatomical parameters of chrysanthemum plants (Excel, Sheer Purple, and William Seward varieties), as well as their genetic stability after 12 months of cold storage under in vitro gene bank conditions. In vitro microshoot tips (without leaves) were placed on a 1/4 Murashige and Skoog medium supplemented with 60.0 g/L sucrose and 0.2–0.8 g/L chlorocholine chloride. The explants were stored in cooling chambers at a temperature of 4 °C. After 12 months of preservation, a reduction was observed in growth kinetics with increasing concentration of chlorocholine chloride in the medium. The viability of explants decreased from 96 to 91% at higher concentrations of chlorocholine chloride. The effective photochemical quantum yield of photosystem II decreased more than twice under cold storage conditions as compared to the control. Several histological features were identified in the leaves of in vitro explants cold-stored in the gene bank: decreased in leaf thickness and mesophyll density in leaf blades, degradation of palisade tissue, and a high degree of transpiration regulation. The SSR and ISSR analyses were used to show that the microshoots of the examined chrysanthemum varieties were identical to the original plants following in vitro cold storage. The conducted studies revealed that the presence of 0.6–0.8 g/L chlorocholine chloride in the medium reduces the growth kinetics of explants while maintaining their high viability and genetic stability.","PeriodicalId":20677,"journal":{"name":"Proceedings of Universities. Applied Chemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New smart thermosensitive biomaterials on the basis of collagen modified by ethylene glycol vinyl glycidyl ether for 4D bioprinting 基于乙二醇乙烯基缩水甘油醚修饰的胶原蛋白的新型智能热敏生物材料，可用于 4D 生物打印

Proceedings of Universities. Applied Chemistry and Biotechnology Pub Date : 2023-12-26 DOI: 10.21285/2227-2925-2023-13-4-469-475

I. A. Farion, A. S. Buinov, A. N. Nikishina, V. F. Burdukovskii

{"title":"New smart thermosensitive biomaterials on the basis of collagen modified by ethylene glycol vinyl glycidyl ether for 4D bioprinting","authors":"I. A. Farion, A. S. Buinov, A. N. Nikishina, V. F. Burdukovskii","doi":"10.21285/2227-2925-2023-13-4-469-475","DOIUrl":"https://doi.org/10.21285/2227-2925-2023-13-4-469-475","url":null,"abstract":"In this study, collagen reactive under UV radiation was obtained for the first time via the functionalization of the side amino groups of lysine moieties with unsaturated ethylene glycol vinyl glycidyl ether (Vinylox) containing, similarly to glycidyl methacrylate, an epoxy group. The presence of this group having a moderate reactivity provides selective grafting of the side amino groups of collagen in a neutral or weakly alkaline medium and excludes the formation of by-products in the Michael reaction and hydrolysis characteristic of the analog—glycidyl methacrylate. Photometry data indicate that with modification in a weakly basic aqueous solution at room temperature and a significant excess of Vinylox, a grafting degree of 23.4% can be achieved. For the first time, film-forming composites capable of photocuring were obtained by adding polyethylene glycol diacrylate and poly(N-isopropylacrylamide) to a weakly acidic aqueous solution of collagen. The presence of vinyl oxide groups provided satisfactory mechanical characteristics of films as the result of UV-initiated collagen cross-linking, while the presence of poly(N-isopropylacrylamide) ensured hydrophilic-hydrophobic smart sensitivity. The films have a well-developed fibrillar structure, and the size of voids enables the free movement of nutrients and other compounds. According to MTT assay data, the films release no cytotoxic components and maintain the metabolic activity of stem cells, providing sufficient density of stem cells on their surface. All of the factors mentioned above determine the prospects of using the films both as an artificial extracellular matrix scaffold and as smart thermosensitive scaffolds used to grow stem cells on their surfaces for subsequent laser transfer bioprinting.","PeriodicalId":20677,"journal":{"name":"Proceedings of Universities. Applied Chemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of protatranes on the biosynthesis of extracellular enzymes of Candida ethanolica BKM Y-2300 T 原烷对乙醇念珠菌 BKM Y-2300 T 细胞外酶生物合成的影响

Proceedings of Universities. Applied Chemistry and Biotechnology Pub Date : 2023-12-26 DOI: 10.21285/2227-2925-2023-13-4-506-515

A. S. Kiryukhina, T. S. Lozovaya, S. N. Adamovich

{"title":"Effect of protatranes on the biosynthesis of extracellular enzymes of Candida ethanolica BKM Y-2300 T","authors":"A. S. Kiryukhina, T. S. Lozovaya, S. N. Adamovich","doi":"10.21285/2227-2925-2023-13-4-506-515","DOIUrl":"https://doi.org/10.21285/2227-2925-2023-13-4-506-515","url":null,"abstract":"The study aims to explore the possibility of using synthetic biologically active compounds (protatranes) to increase the lipolytic and proteolytic activity of Candida ethanolica. Protatrane 1 (tris(2-hydroxyethyl) ammonium-4chlorophenyl-sulfanyl acetate) and protatrane 2 (tris(2-hydroxyethyl) ammonium-4-chlorophenyl-sulfonyl acetate) were added to the growth medium at trace concentrations of 1×10−6–1×10−8wt% separately and together. It was established that with the introduction to the growth medium, protatranes had a multidirectional effect on the biosynthesis of enzymes by Candida ethanolica yeast. This effect was dependent on the concentrations of these compounds, as well as on their separate or combined introduction. All the studied concentrations were found to be effective for lipase synthesis, as they improved enzyme synthesis by 1.7–8.6 times. The combined use of protatranes increased enzyme synthesis by 3.4–11.7 times. For protease formation, the combined introduction of the studied protatranes at a concentration of 10−6wt% was found to be the most effective, with enzyme synthesis reaching 184.8±7.02 U/mL in the culture broth. When co-introduced, the studied protatranes tris(2-hydroxyethyl) ammonium-4-chlorophenylsulfanyl acetate and tris(2-hydroxyethyl) ammonium-4-chlorophenyl-sulfonyl acetate can be used to increase the production efficiency of extracellular lipases and proteases by Candida ethanolica. The specific effect of protatranes was revealed to be the uneven dynamics of extracellular enzyme accumulation, manifested in the presence of sharp peaks during yeast cultivation. The reasons for this unevenness require further research.","PeriodicalId":20677,"journal":{"name":"Proceedings of Universities. Applied Chemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and antinociceptive activity of 2,2’-(1,4-phenylene)bis[3-aryl-2-azaspiro[3.5]nonan-1-ones] 2,2'-(1,4-亚苯基)双[3-芳基-2-氮杂螺[3.5]壬-1-酮]的合成和抗镇痛活性

Proceedings of Universities. Applied Chemistry and Biotechnology Pub Date : 2023-12-26 DOI: 10.21285/2227-2925-2023-13-4-476-482

R. R. Makhmudov, E. A. Nikiforova, D. P. Zverev, L. A. Balyukina, A. Р. Skachkov

{"title":"Synthesis and antinociceptive activity of 2,2’-(1,4-phenylene)bis[3-aryl-2-azaspiro[3.5]nonan-1-ones]","authors":"R. R. Makhmudov, E. A. Nikiforova, D. P. Zverev, L. A. Balyukina, A. Р. Skachkov","doi":"10.21285/2227-2925-2023-13-4-476-482","DOIUrl":"https://doi.org/10.21285/2227-2925-2023-13-4-476-482","url":null,"abstract":"Over the years, azetidin-2-ones, or β-lactams, have received a lot of attention from scientists as potential drug candidates due to their diverse biological activity. Spiro-β-lactams also exhibit biological activity; therefore, it is of interest to synthesize and study the properties of new compounds belonging to this class. The study aims to examine the antinociceptive activity of several synthesized bis(spirolactams), specifically 2,2’-(1,4-phenylene)bis[3-aryl-2azaspiro[3.5]nonan-1-ones]. These bis(spiroazetidine-2-ones) were obtained in the interaction of a twofold excess of the Reformatsky reagent, derived from methyl 1-bromocyclohexane carboxylate and zinc, with N,N-(1,4-phenyle- ne)bis(1-arylmethanimines) by means of boiling them in a 10:1 mixture of toluene and hexamethylphosphorictriamide for four hours. Bis(spiro-β-lactams) on the basis of diimines derived from p-phenylenediamine, 2-methoxybenzaldehyde, p-tolualdehyde, and 3-bromobenzoic aldehyde were synthesized for the first time. The composition and structure of the previously undescribed products were established using IR, [1]H, and 13C NMR spectroscopy and elemental analysis. The antinociceptive activity of the obtained compounds was studied on outbred white mice of both sexes via the hot plate test with an intraperitoneal injection. The effect was estimated two hours after administration. Several synthesized compounds were found to exhibit antinociceptive activity at or above the level of the comparator product – metamizole sodium. Nerve endings can be considered the target of the antinociceptive activity of examined substances since under the effect of these substances, no signs of central action are observed in the behavior of animals. Thus, the conducted studies showed the promise of further search for biologically active substances among the compounds of this series.","PeriodicalId":20677,"journal":{"name":"Proceedings of Universities. Applied Chemistry and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0