Proceedings of the Society for Experimental Biology and Medicine最新文献

{"title":"INDOCYANINE GREEN-PLASMA HALF TIME CLEARANCE (T 1/2) IN NORMAL BEAGLES.","authors":"W J KRASAVAGE, S M MICHAELSON","doi":"10.3181/00379727-119-30140","DOIUrl":"https://doi.org/10.3181/00379727-119-30140","url":null,"abstract":"Summary 1. The mean plasma clearance half time of indocyanine green (ICG) was found to be 5.6 ± 0,8§ minutes in male beagles, and 7.0 ± 1.48 in female beagles, 2. Female dogs in this study cleared ICG significantly more slowly than the males. 3. Plasma volume estimated using ICG is 3 7.7 ± 6.0 ml/kg. This figure agrees with 37.5 ± 6.1 ml/kg found using radioiron (Fe-59).","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"215-8"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHROMOSOME CHANGES IN PPLO-INFECTED FL HUMAN AMNION CELLS.","authors":"J FOGH, H FOGH","doi":"10.3181/00379727-119-30145","DOIUrl":"https://doi.org/10.3181/00379727-119-30145","url":null,"abstract":"Summary Characteristic chromosome changes were observed in PPLO-infected FL human amnion cells. These changes included a gradual reduction in chromosome numbers, increase in chromosome aberrations, and the appearance of 3 new varieties. Although some of the aberrations appeared early after infection, most changes developed slowly, over a period of several months.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"233-8"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SOME ENDOGENOUS PARATHYROID HORMONE EFFECTS MANIFESTED BY BONE IN VITRO.","authors":"C W COOPER, C W YATES, R V TALMAGE","doi":"10.3181/00379727-119-30104","DOIUrl":"https://doi.org/10.3181/00379727-119-30104","url":null,"abstract":"Summary A system of bone incubation is described in which pooled rat serum was used for the incubation of femoral fragments. The following results were observed: 1. In vitro calcium and Ca45 equilibration studies on metaphyseal and diaphyseal femoral rat bone indicate that metaphyseal bone equilibrates at a higher level than does diaphyseal bone. 2. Incubated bone taken from parathyroid intact animals maintains higher calcium levels in vitro than bone from parathyroidectomized rats; this is also the case for Ca45 injected longer than 24 hours before sacrifice and subsequent incubation. If the Ca45 is injected less than 24 hours before sacrifice, the same amount of radioactivity is released into the medium for both intact and parathyroidectomized groups. Dead bone, however, always releases less radioactivity than live bone. 3. These studies indicate that endogenous parathyroid hormonal effects are detectable when bone is removed from parathyroid intact rats and incubated. These effects are exerted through an influence on cellular mediated processes; however, bone from parathyroidectomized rats also maintains media calcium levels partly through energy requiring processes. 4. Evidence presented illustrates 2 distinct processes of bone salt removal exist, one of which is influenced by endogenous parathyroid hormone. Data also imply that diaphyseal (compact) bone may be the primary site of parathyroid hormone influences on calcium homeostasis.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"81-8"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40798138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INOTROPIC EFFECTS OF OUABAIN ON RABBIT LEFT ATRIA IN PRESENCE OF BETA-ADRENERGIC BLOCKING DRUGS.","authors":"J V LEVY, V RICHARDS","doi":"10.3181/00379727-119-30158","DOIUrl":"https://doi.org/10.3181/00379727-119-30158","url":null,"abstract":"Summary The positive inotropic effects of ouabain on electrically driven rabbit left atrial preparations was studied in the presence of 3 drugs known to be potent and specific beta-adrenergic receptor antagonists (pronethalol, propranolol and MJ-1999). Ouabain alone (6.9 × 10-7 M) produced a highly significant increase in contraction force compared to untreated controls. Pronethalol (3.76 × 10-6 M) and propranolol (1.35 × 10-6 M) were found to produce significant decreases in contraction force. The methanesulfonanilide substituted isopropylphenylethylamine (MJ-1999) had no significant effects on contraction force in a concentration of 1.3 × 10-5 M. Administering ouabain after pretreatment with and in the presence of either pronethalol, propranolol, or MJ-1999 failed to modify significantly the normal positive inotropic effect of the glycoside. Therefore, no evidence was found to indicate that beta-adrenergic receptor blockade could prevent the positive inotropic effect of ouabain on isolated rabbit left atria.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"278-81"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40796562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SURVIVAL OF TYPE 1 AND TYPE 3 POLIO VACCINE VIRUS IN BLOWFLIES. (PHAENICIA SERICATA) AT 40 DEGREE C.","authors":"K H DAVE, R C WALLIS","doi":"10.3181/00379727-119-30114","DOIUrl":"https://doi.org/10.3181/00379727-119-30114","url":null,"abstract":"Discussion and summary Interest in the behavior of polioviruses in flies stems from two factors: (1) the potential importance of these insects as vectors of the virus under certain circumstances; and (2) the possibility that if polioviruses multiply in flies, they may undergo mutation during the process. Investigations of the genetic stability of polioviruses have been carried out largely in connection with the use of attenuated vaccine strains as immunizing agents for humans. It is apparent that wild virulent strains multiply readily at 40°C, while attenuated strains, either naturally occurring or laboratory manipulated vaccine strains, do not. In tropical areas, and in temperate climates during the summer, flies are exposed to high environmental temperatures at least for some time during the day. If this occurs while they are hosts of polioviruses, and if polioviruses undergo multiplication in flies, it is possible that attenuated strains might undergo changes in their genetic characters, with a shift toward the pattern of greater virulence. The results of the present investigation, which was undertaken to explore this possibility, indicate that under the conditions tested there was no evidence of multiplication in type 1 or type 3 attenuated vaccine strains (Sabin) in the fly species Phaenicia sericata. Poliovirus was recovered in gradually diminishing quantities for as long as 15 days from flies which had been fed type 1 virus and for at least 13 days from those which had received type 3. When certain genetic characters of the virus strains isolated from 2 to 15 days after virus feeding were compared with those of the type 1 and 3 vaccine strains, they were found to be identical; all isolates tested proved to be rct/40°C negative and vaccine-like in the intratypic serodifferentiation test of McBride. Thus the results do not provide quantitative evidence for poliovirus multiplication in the fly Phaenicia sericata, nor was it possible to demonstrate any shift in genetic characters suggesting changes toward a pattern of more virulent strains of virus.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"121-4"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40799769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INFLUENCE OF PERITONEAL INFLAMMATORY EXUDATE ON DEVELOPMENT OF A TRANSPLANTABLE MOUSE ASCITES TUMOR.","authors":"G BARSKI, J BELEHRADEK, J KAWAMATA","doi":"10.3181/00379727-119-30111","DOIUrl":"https://doi.org/10.3181/00379727-119-30111","url":null,"abstract":"Summary Three daily peritoneal injections of 1:1000 glycogen solution induced in mice, during at least 10 days, a state of relative resistance to intraperitoneal but not subcutaneous inoculation of homotransplantable dL-46 ascites tumor cells. Removal of excess macrophages by peritoneal washing or their transfer to untreated mice did not influence the development of dL-46 ascites. Furthermore, elaboration of specific immune reaction by intraperitoneal inoculation of irradiated cells was not enhanced in glycogen treated mice.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"111-4"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40885492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VINBLASTINE EFFECT ON NUCLEIC ACIDS AND RIBONUCLEASE OF LUMPHOID TISSUE.","authors":"P H WIERNIK, R M MACLEOD","doi":"10.3181/00379727-119-30113","DOIUrl":"https://doi.org/10.3181/00379727-119-30113","url":null,"abstract":"Summary The RNA concentration and ribonuclease activity in rat thymus were decreased by injection of vinblastine. The same results were noted in both the corticoid sensitive and resistant strains of mouse lymphosarcoma P1798. An apparent increase in DNA concentration was noted in rat thymus and corticoid-sensitive P1798. No change in DNA concentration was noted in the corti-coid-resistant tumor.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"118-20"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40885494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRELIMINARY COMMUNICATION ON THE SURVIVAL, THE SITES HARBORING VIRUS AND GENETIC STABILITY OF POLIOVIRUS IN COCKROACHES.","authors":"K H DAVE, R C WALLIS","doi":"10.3181/00379727-119-30115","DOIUrl":"https://doi.org/10.3181/00379727-119-30115","url":null,"abstract":"Summary Sabin's attenuated strain of poliovirus type 3 persisted in cockroaches (Blaberus discoidalis) after experimental infection for as long as 51 days. No evidence was obtained indicating multiplication of the virus. Rather, the pattern was one of mechanical carriage of the virus with a gradual decline in titre. It was possible to recover virus only from the gastrointestinal tract of roaches dissected 27 days after infection but not from nerve trunk or other parts of the body of the roaches.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"124-6"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40885495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRANSFER OF GAMMA GLOBULIN FROM MOTHER TO OFFSPRING IN MINK.","authors":"D D PORTER","doi":"10.3181/00379727-119-30117","DOIUrl":"https://doi.org/10.3181/00379727-119-30117","url":null,"abstract":"Summary Newborn mink have no detectable gamma globulin, and acquire gamma globulin from the mother via the colostrum and milk. Gamma globulin concentration in the colostrum was less than that of the maternal serum.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"131-3"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40885498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BODY AND MUSCLE WEIGHT DEPRESSING EFFECTS AND THYMOLYTIC POTENCIES OF GLUCOCORTICOIDS IN THE RAT.","authors":"G TONELLI, R PARTRIDGE, I RINGLER","doi":"10.3181/00379727-119-30120","DOIUrl":"https://doi.org/10.3181/00379727-119-30120","url":null,"abstract":"Summary The effects of hydrocortisone, prednisolone, methylprednisolone, dexamethasone or triamcinolone on body weights, the weights of the rectus femoris, triceps brachialis and gastrocnemius muscles and food consumption were studied in normal male rats, weighing 87–111 g. Subcutaneous administration of the aforementioned glucocorticoids, once a day for 10 consecutive days, elicited a reduction in body weights, muscle weights and food consumption which appeared roughly proportional to the relative thymolytic potency of each glucocorticoid. From pair-feeding experiments, it was apparent that the reduction in food consumption accounted for 53–65% of the total body weight losses. Muscle weight losses were similarly affected. It was concluded that glucocorticoids have similar quantitative effects on body and muscle weights of the rat if administered at equipotent thymolytic doses.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"136-42"},"PeriodicalIF":0.0,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30120","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40885501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}