Se pu = Chinese journal of chromatography最新文献

[Impact of carrier flow composition on membrane adsorption and aggregation of ovalbumin in asymmetrical flow field-flow fractionation]. [载体流动组成对不对称流场-流分馏法中卵清蛋白膜吸附和聚集的影响]。

IF 0.7

Se pu = Chinese journal of chromatography Pub Date : 2018-05-08 DOI: 10.3724/SP.J.1123.2017.12014

Qihui Liang, Yi Yang, Bing Shao, Ye Gao, Yu Song, Nanyin Han

{"title":"[Impact of carrier flow composition on membrane adsorption and aggregation of ovalbumin in asymmetrical flow field-flow fractionation].","authors":"Qihui Liang, Yi Yang, Bing Shao, Ye Gao, Yu Song, Nanyin Han","doi":"10.3724/SP.J.1123.2017.12014","DOIUrl":"https://doi.org/10.3724/SP.J.1123.2017.12014","url":null,"abstract":"Asymmetrical flow field-flow fractionation (AF4) is a kind of moderate separation technology for the analysis of macromolecules, including proteins with a wide range of sizes. In the separation channel, the membrane adsorption and aggregation of proteins affected by the carrier fluid (CF) composition lead to changes in analyte recovery and size distribution, thereby restricting the application of AF4 to biomolecules. Different pH levels (6.2, 7.4, 8.2), several types of cations (Na+, K+, Mg2+) and various ion strengths (0-0.1 mol/L)were studied to demonstrate the influence of carrier fluid composition on the membrane adsorption and aggregation of proteins. The results revealed the following:a) higher ion strength of CF resulted in a greater degree of membrane adsorption and aggregation; b) the zeta potential, determined by the pI of the protein and the pH of the CF, influenced the adsorption and aggregation; c) divalent cations (Mg2+) caused serious adsorption and aggregation. The experimental results can help us achieve better recovery and mitigate aggregate formation by using the optimal CF components in future AF4 studies. Moreover, the findings indicate that AF4 would find extensive application in protein biochemistry assays.","PeriodicalId":206652,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"36 5","pages":"480-486"},"PeriodicalIF":0.7,"publicationDate":"2018-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36420262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Fast separation and analysis of water-soluble vitamins in spinach by capillary electrophoresis with high voltage]. [高压毛细管电泳快速分离分析菠菜中水溶性维生素]。

IF 0.7

Se pu = Chinese journal of chromatography Pub Date : 2009-11-01

Xiaoqin Hu, Huiyan You

引用次数: 0

[Simultaneous determination of four carotenoids in health products by reversed-phase high performance liquid chromatography]. [反相高效液相色谱法同时测定保健品中4种类胡萝卜素]。

IF 0.7

Se pu = Chinese journal of chromatography Pub Date : 2006-09-01

He Li, Min Chen, Lei Zhu, Lijuan Liu, Jingyu Wang

{"title":"[Simultaneous determination of four carotenoids in health products by reversed-phase high performance liquid chromatography].","authors":"He Li, Min Chen, Lei Zhu, Lijuan Liu, Jingyu Wang","doi":"","DOIUrl":"","url":null,"abstract":"A method was established for the determination of carotenoids (lutein, zeaxanthin, lycopene, beta-carotene) in health products by reversed-phase high performance liquid chromatography (RP-HPLC). The extraction methods of carotenoids from different types of samples were also established. Separation and quantification was achieved by using a Diamonsil C18 column (250 mm x 4.6 mm i.d., 5 microm), and a mobile phase of acetonitrile and ethyl acetate were used with the following gradient elution: 100% acetonitrile at the first 15 min, then ethyl acetate increased to 100% in the next 10 min, at last 100% acetonitrile for 5 min with a flow rate of 1 mL/min, temperature of 25 degrees C and monitored with a photodiode array detector at 450 nm. The external standard calibration curves were used for quantification. There were good linear correlations for the four carotenoids. The correlation coefficients were between 0.999 4 and 0. 999 7. The detection limits were 0.4-0.5 mg/L. The recoveries of four carotenoids in tablet samples were 95.3%-98.7% with the relative standard deviations (RSDs) of 0.89%-2.0%. The recoveries of four carotenoids in powder samples were 93.7%-98.8% with RSDs of 0.89%-2.8%. The recoveries of four carotenoids in oil samples were 97.1%-99.2% with RSDs of 0.42%-1.2%. This method is simple, fast, accurate and convenient. It can be used as one of the reliable means for quantitative determination of carotenoid in health products.","PeriodicalId":206652,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"24 5","pages":"475-8"},"PeriodicalIF":0.7,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26495408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Quantitative structure-retention relationships of monosubstituted alkanes by dividing its molecular structure into substructure]. [单取代烷烃分子结构分亚结构的定量结构-保留关系]。

IF 0.7

Se pu = Chinese journal of chromatography Pub Date : 2005-07-01

Chenzhong Cao, Ping Huo, Shuo Gao, Zaichun Zhou

引用次数: 0

[Application of genetic algorithm in optimization of mobile phase composition in high performance liquid chromatography]. [遗传算法在高效液相色谱流动相组成优化中的应用]。

IF 0.7

Se pu = Chinese journal of chromatography Pub Date : 2002-03-01

Xue-Guo Chen, Jian-Yi Ni, Han-Fa Zou, Rui-Huan Zhao

引用次数: 0