[Fast separation and analysis of water-soluble vitamins in spinach by capillary electrophoresis with high voltage].

Xiaoqin Hu, Huiyan You
{"title":"[Fast separation and analysis of water-soluble vitamins in spinach by capillary electrophoresis with high voltage].","authors":"Xiaoqin Hu,&nbsp;Huiyan You","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>In capillary electrophoresis, 0-40 kV (even higher) voltage can be reached by a connecting double-model high voltage power supply. In the article, water-soluble vitamins, VB1, VB2, VB6, VC, calcium D-pantothenate, D-biotin, nicotinic acid and folic acid in vegetable, were separated by using the high voltage power supply under the condition of electrolyte water solution as running buffer. The separation conditions, such as voltage, the concentration of buffer and pH value etc. , were optimized during the experiments. The results showed that eight water-soluble vitamins could be baseline separated in 2.2 min at 40 kV applied voltage, 25 mmol/L sodium tetraborate buffer solution (pH 8.8). The water-soluble vitamins in spinach were quantified and the results were satisfied. The linear correlation coefficients of the water-soluble vitamins ranged from 0.9981 to 0.9999. The detection limits ranged from 0.2 to 0.3 mg/L. The average recoveries ranged from 88.0% to 100.6% with the relative standard deviations (RSD) range of 1.15%-4.13% for the spinach samples.</p>","PeriodicalId":206652,"journal":{"name":"Se pu = Chinese journal of chromatography","volume":"27 6","pages":"835-9"},"PeriodicalIF":0.0000,"publicationDate":"2009-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Se pu = Chinese journal of chromatography","FirstCategoryId":"92","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

In capillary electrophoresis, 0-40 kV (even higher) voltage can be reached by a connecting double-model high voltage power supply. In the article, water-soluble vitamins, VB1, VB2, VB6, VC, calcium D-pantothenate, D-biotin, nicotinic acid and folic acid in vegetable, were separated by using the high voltage power supply under the condition of electrolyte water solution as running buffer. The separation conditions, such as voltage, the concentration of buffer and pH value etc. , were optimized during the experiments. The results showed that eight water-soluble vitamins could be baseline separated in 2.2 min at 40 kV applied voltage, 25 mmol/L sodium tetraborate buffer solution (pH 8.8). The water-soluble vitamins in spinach were quantified and the results were satisfied. The linear correlation coefficients of the water-soluble vitamins ranged from 0.9981 to 0.9999. The detection limits ranged from 0.2 to 0.3 mg/L. The average recoveries ranged from 88.0% to 100.6% with the relative standard deviations (RSD) range of 1.15%-4.13% for the spinach samples.

[高压毛细管电泳快速分离分析菠菜中水溶性维生素]。
在毛细管电泳中,通过连接双模高压电源可以达到0- 40kv(甚至更高)的电压。本文采用高压电源,在电解水溶液作为运行缓冲液的条件下,对蔬菜中水溶性维生素VB1、VB2、VB6、VC、d -泛酸钙、d -生物素、烟酸和叶酸进行了分离。实验中对分离条件如电压、缓冲液浓度、pH值等进行了优化。结果表明,在40 kV电压、25 mmol/L四硼酸钠缓冲溶液(pH 8.8)条件下,8种水溶性维生素可在2.2 min内基线分离。对菠菜中的水溶性维生素进行了定量分析,结果令人满意。水溶性维生素的线性相关系数在0.9981 ~ 0.9999之间。检出限为0.2 ~ 0.3 mg/L。加样回收率为88.0% ~ 100.6%,相对标准偏差(RSD)为1.15% ~ 4.13%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信