Phycological Research最新文献_第9页

Immediate response of Chara braunii exposed to zinc and hydrogen peroxide 暴露在锌和过氧化氢中的布拉蛙的即时反应

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-11-04 DOI: 10.1111/pre.12471

A. Herbst, V. Ranawakage, T. Asaeda, H. Schubert

{"title":"Immediate response of Chara braunii exposed to zinc and hydrogen peroxide","authors":"A. Herbst, V. Ranawakage, T. Asaeda, H. Schubert","doi":"10.1111/pre.12471","DOIUrl":"https://doi.org/10.1111/pre.12471","url":null,"abstract":"The immediate effect of zinc (Zn) and hydrogen peroxide (H2O2) in Chara braunii was analyzed in short‐time exposure experiments. The exposure concentrations were 12.3, 18.4, and 24.5 μmol L−1 H2O2, 12, 60, and 120 mg L−1 Zn, and 12.3 μmol L−1 H2O2 + 12 mg L−1 Zn, 12.3 μmol L−1 H2O2 + 60 mg L−1 Zn, and 18.4 μmol L−1 H2O2 + 12 mg L−1 Zn. The stress response of C. braunii was analyzed by measuring photosynthetic photosystem II activity, chlorophyll a and b and carotenoid contents, the H2O2 concentration, and antioxidant enzyme activities of ascorbic peroxidase, catalase, and guaiacol peroxidase. The short‐term addition of Zn reduced pigment contents in C. braunii. Chlorophyll a and b and carotenoid contents in H2O2‐exposed C. braunii were as high as in control plants. Photosynthesis was reduced in H2O2‐treated C. braunii and the short‐term addition of Zn did not affect the electron transport rate. H2O2 concentration and antioxidant enzyme activities in C. braunii were not significantly different between control and exposed plants. Trends of enzymatic adaptation were described: the H2O2‐induced stress response was characterized by increased antioxidant enzyme activities, whereas Zn inactivated catalase in C. braunii.","PeriodicalId":20544,"journal":{"name":"Phycological Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46441857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome editing using a DNA‐free clustered regularly interspaced short palindromic repeats‐Cas9 system in green seaweed Ulva prolifera 使用无DNA集群规则间隔短回文重复序列- Cas9系统在绿海藻Ulva prolifera进行基因组编辑

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-11-03 DOI: 10.1111/pre.12472

K. Ichihara, T. Yamazaki, S. Kawano

{"title":"Genome editing using a DNA‐free clustered regularly interspaced short palindromic repeats‐Cas9 system in green seaweed Ulva prolifera","authors":"K. Ichihara, T. Yamazaki, S. Kawano","doi":"10.1111/pre.12472","DOIUrl":"https://doi.org/10.1111/pre.12472","url":null,"abstract":"Although the green seaweed Ulva is one of the most common seaweeds in the coastal regions with well‐studied ecological characteristics, few reverse genetic technologies have been developed for it. The clustered regularly interspaced short palindromic repeats (CRISPR)‐Cas9 system is a simple genome‐editing technology based on a ribonucleoprotein (RNP) complex composed of an endonuclease and programmable RNA to target particular DNA sequences. Genome editing makes it possible to generate mutations on a target gene in non‐model organisms without established transgenic technologies. In this study, we applied the CRISPR‐Cas9 RNP genome‐editing system to the green seaweed Ulva prolifera, using polyethylene glycol (PEG)‐mediated transfection. Our experimental system disrupts a single gene (UpAPT) encoding adenine phosphoribosyl transferase (APT) and generates a resistant phenotype for gametophytes cultured in a medium with toxic compound 2‐fluoroadenine. The PEG‐mediated transfection used for gametes resulted in 2‐fluoroadenine‐resistant strains containing short indels or substitutions on UpAPT. Our results showed that the CRISPR‐Cas9 system with PEG‐mediated transfection was efficient for genome editing in Ulva.","PeriodicalId":20544,"journal":{"name":"Phycological Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41939152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Diatom (Bacillariophyceae) assemblages in tidal environments of Vancouver Island, British Columbia, Canada 加拿大不列颠哥伦比亚省温哥华岛潮汐环境中的硅藻（硅藻科）组合

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-10-29 DOI: 10.1111/pre.12470

Y. Sawai, K. Tanigawa, T. Shinozaki, P. Bobrowsky, D. Huntley, James Goff

{"title":"Diatom (Bacillariophyceae) assemblages in tidal environments of Vancouver Island, British Columbia, Canada","authors":"Y. Sawai, K. Tanigawa, T. Shinozaki, P. Bobrowsky, D. Huntley, James Goff","doi":"10.1111/pre.12470","DOIUrl":"https://doi.org/10.1111/pre.12470","url":null,"abstract":"To understand distributions of coastal diatoms along Vancouver Island, British Columbia, Canada, this paper describes diatom assemblages observed in 47 surface sediment samples from intertidal environments. One hundred and eighty‐four diatom taxa were identified from five transects crossing tidal flats, salt marshes, and freshwater forests in Tofino, Ucluelet, and Port Alberni. Distributions of the diatom assemblages were consistent with those reported elsewhere in the Pacific Northwest, but a few diatom taxa show different trends in their distributions. For example, one benthic species Denticula subtilis shows widespread distributions along the transect in Tofino. An ordination shown by Detrended Correspondence Analysis (DCA) using a combined dataset indicated overlapped scatter plots of diatom assemblages, suggesting that assemblages with similar species compositions are observed in more than one location. Hierarchical and k‐means clustering analyses using Euclidean distance recognized unique small groups along each transect. Rank abundance curves show different trends for richness and evenness of diatom assemblages among the five transects.","PeriodicalId":20544,"journal":{"name":"Phycological Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45562602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New species Micractinium kostikovii (Chlorellaceae, Trebouxiophyceae) from Russia 俄罗斯的新种小球藻Micractinium kostikovii（小球藻科、树叶藻科）

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-10-17 DOI: 10.1111/pre.12469

E. Krivina, A. Temraleeva, M. Sinetova

引用次数: 6

Issue Information 问题信息

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-10-01 DOI: 10.1111/pre.12429

引用次数: 0

Novelties in nomenclature and typification appearing in Phycological Research 69 (4) 命名法和典型化方面的创新出现在《生态学研究》69（4）

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-10-01 DOI: 10.1111/pre.12468

引用次数: 0

First record of Neoporphyra haitanensis (T.J.Chang & B.F.Zheng) J.Brodie & L.‐E.Yang (Bangiales, Rhodophyta) from Shikinejima Island and Hachijojima Island of the Izu Islands, southern central Japan 日本中南部伊豆列岛岛和八重岛的坛新豚（T.J.Chang&B.F.Zheng）J.Brodie&L.‐E.Yang（Bangiales，Rhodophyta）的首次记录

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-10-01 DOI: 10.1111/pre.12466

F. Sano, Airi Ikeura, A. Takasugi, K. Niwa

{"title":"First record of Neoporphyra haitanensis (T.J.Chang & B.F.Zheng) J.Brodie & L.‐E.Yang (Bangiales, Rhodophyta) from Shikinejima Island and Hachijojima Island of the Izu Islands, southern central Japan","authors":"F. Sano, Airi Ikeura, A. Takasugi, K. Niwa","doi":"10.1111/pre.12466","DOIUrl":"https://doi.org/10.1111/pre.12466","url":null,"abstract":"We conducted a morphological observation and molecular phylogenetic analyses on bladed Bangiales collected from natural populations on Shikinejima Island and Hachijojima Island of the Izu Islands, southern central Japan. The morphological features of the gametophytic blades such as the blade shape, blade margin and reproductive cell divisions were extremely similar to those parameters of Neoporphyra haitanensis, a species cultivated in southern China, and Neoporphyra dentata. However, the blades collected from the two islands were thicker than the blades of N. dentata; this feature was the same as that of N. haitanensis. The chloroplast RUBISCO spacer sequences of present samples were identical to the sequences of N. haitanensis from China. Moreover, phylogenetic positions of three conchocelis strains isolated from the blades on the two islands were examined based on concatenated sequences of the chloroplast rbcL gene and nuclear 18S rRNA. The results showed that the strains were resolved in a clade including N. haitanensis; however, the strains were separated from N. haitanensis in China. Therefore, the bladed Bangiales from the two islands were identified as N. haitanensis. The present study is the first record of N. haitanensis in natural populations in Japan.","PeriodicalId":20544,"journal":{"name":"Phycological Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/pre.12466","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45602678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Seasonal variation of carrageenan yield, gel strength and viscosity in Sarcopeltis (ex Gigartina) skottsbergii from Southern Chile 智利南部斯科茨贝氏Sarcopeltis (ex Gigartina)卡拉胶产率、凝胶强度和粘度的季节变化

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-09-29 DOI: 10.1111/pre.12467

R. Westermeier, Carmen González, P. Murúa, J. Morales, David J. Patiño, Natacha Fabres, Jaime Zamorano, D. Müller

{"title":"Seasonal variation of carrageenan yield, gel strength and viscosity in Sarcopeltis (ex Gigartina) skottsbergii from Southern Chile","authors":"R. Westermeier, Carmen González, P. Murúa, J. Morales, David J. Patiño, Natacha Fabres, Jaime Zamorano, D. Müller","doi":"10.1111/pre.12467","DOIUrl":"https://doi.org/10.1111/pre.12467","url":null,"abstract":"Chile is one of the top carrageenan producers worldwide, and Sarcopeltis (ex Gigartina) skottsbergii one of the topmost exploited carrageenophytes from the wild in the world. Total yield, gel strength and viscosity from two contrasting environments Calbuco and Ancud (Inner and Outer Sea, Chile) were estimated monthly in approximately 2 years for this species. While carrageenan yields did not show differences between localities, gametophytes in spring–summer had 15% higher, compared to tetrasporophytes. Sizes (frond surface) normally did not affect carrageenan yields. Gametophytes showed clear differences in gel strength between seasons, but not between localities, with maximum peaks during winter–spring months in Calbuco and autumn‐winter months in Ancud. Seasonal variations in viscosity were also significant. While gametophyte viscosity did not exceed 120 cPs, tetrasporophytes reached 1400 cPs in Calbuco and 1000 cPs in Ancud. More remarkably, a positive correlation between viscosity and gel strength was found in S. skottsbergii gametophytes, which is significantly different between both localities. These results suggest that selective harvesting in spring–summer should be preferred to optimize cost–benefit of harvesting activities and subsequent carrageenan productivity.","PeriodicalId":20544,"journal":{"name":"Phycological Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48693815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The effect of drying on undervalued brown and red seaweed species: Bioactivity alterations 干燥对被低估的褐藻和红藻的影响:生物活性的改变

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-07-26 DOI: 10.1111/pre.12465

D. Julião, C. Afonso, A. Gomes‐Bispo, N. Bandarra, C. Cardoso

{"title":"The effect of drying on undervalued brown and red seaweed species: Bioactivity alterations","authors":"D. Julião, C. Afonso, A. Gomes‐Bispo, N. Bandarra, C. Cardoso","doi":"10.1111/pre.12465","DOIUrl":"https://doi.org/10.1111/pre.12465","url":null,"abstract":"The effect of drying on two brown seaweed (Treptacantha abies‐marina and Cystoseira humilis) and two red seaweed species (Asparagopsis armata and Asparagopsis taxiformis) was evaluated based on the levels of biological activity, such as antioxidant and anti‐inflammatory properties. Moreover, the contents of some significant compound classes, such as polyphenols and beta‐glucans, were determined. Seaweeds C. humilis and T. abies‐marina showed high polyphenol levels (1.76–6.78 mg gallic acid equivalents g−1 dw), which clearly exceeded those determined in the Asparagopsis genus regardless of drying process. This was partially reflected in the antioxidant activity as measured by DPPH and FRAP methods, which showed that extracts from C. humilis and T. abies‐marina in most cases had stronger antioxidant activity than those from Asparagopsis species. The influence of the drying technique upon the antioxidant activity was relatively limited, since in many instances there was no effect. Concerning anti‐inflammatory activity, in the case of shade‐dried samples, C. humilis had a higher activity (>30% COX‐2 inhibition) than A. armata. However, this activity in C. humilis was not rendered available in the upper gastrointestinal (GI) tract. Indeed, only A. taxiformis displayed anti‐inflammatory activity in the fraction available in the upper GI tract, showing an upper GI tract availability in the 90–100% range. Therefore, though bioactivity levels were higher in C. humilis and T. abies‐marina, Asparagopsis species also had substantial levels of bioactive compound. Sun‐drying produced more negative effects than shade‐drying, but these effects were not very extensive.","PeriodicalId":20544,"journal":{"name":"Phycological Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/pre.12465","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43312007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Neoporphyra kitoi sp. nov. (Bangiales, Rhodophyta) from Japan, with new blades arising from rhizoidal cells 产自日本的Neoporphyra kitoi sp. 11 . (Bangiales, rhodophya)，具有由根状细胞产生的新叶片

IF 1.5 4区生物学

Phycological Research Pub Date : 2021-07-15 DOI: 10.1111/pre.12464

Mahiko Abe, N. Kikuchi, M. Tamaki, Tomoko Sato, N. Murase, Eiji Fujiyoshi, Masahiro Kobayashi

引用次数: 6