Preparative biochemistry最新文献_第3页

Joint chromatographic purification of bovine serum ceruloplasmin and amineoxidase. 牛血清铜蓝蛋白和氨基氧化酶的联合层析纯化。

Preparative biochemistry Pub Date : 1994-11-01 DOI: 10.1080/10826069408010096

X T Wang, M J Dumoulin, O Befani, B Mondovi, M A Mateescu

引用次数: 23

Purification and characterization of bovine heart glycogen synthase kinase-3. 牛心糖原合成酶激酶-3的纯化与鉴定。

Preparative biochemistry Pub Date : 1994-11-01 DOI: 10.1080/10826069408010098

S P Henry, S D Killilea

引用次数: 2

A rapid method for the purification of large amounts of an alpha-complementing peptide derived from beta-galactosidase (E. coli). 从β -半乳糖苷酶(大肠杆菌)中提取大量α -互补肽的一种快速纯化方法。

Preparative biochemistry Pub Date : 1994-11-01 DOI: 10.1080/10826069408010101

C N Gallagher, N J Roth, R E Huber

引用次数: 5

Purification and characterization of lysine- and arginine-specific gingivain proteases from Porphyromonas gingivalis. 牙龈卟啉单胞菌中赖氨酸和精氨酸特异性牙龈蛋白酶的纯化和鉴定。

Preparative biochemistry Pub Date : 1994-11-01 DOI: 10.1080/10826069408010097

G S Bedi

引用次数: 10

Rapid purification of yeast cytoplasmic fumarate reductase by affinity chromatography on blue sepharose CL-6B. 蓝葡糖CL-6B亲和层析法快速纯化酵母胞质富马酸还原酶。

Preparative biochemistry Pub Date : 1994-11-01 DOI: 10.1080/10826069408010100

H Muratsubaki, K Enomoto, Y Ichijoh, T Tezuka, T Katsume

引用次数: 13

A novel technique for isolation of pure sperm heads from disintegrated mammalian spermatozoa. 从崩解的哺乳动物精子中分离纯精子头的新技术。

Preparative biochemistry Pub Date : 1994-11-01 DOI: 10.1080/10826069408010092

A R Chaudhuri, H Datta

{"title":"A novel technique for isolation of pure sperm heads from disintegrated mammalian spermatozoa.","authors":"A R Chaudhuri, H Datta","doi":"10.1080/10826069408010092","DOIUrl":"https://doi.org/10.1080/10826069408010092","url":null,"abstract":"The phenomenon of differential charge distribution on sperm surface membrane has been utilised here in a low e.m.f. (electro motive force) capillary electrophoresis system to effect separation of sperm heads from disintegrated mixed spermatozoal subfractions. Washed caudal sperm of goat (Black Bengal variety) and ejaculated washed human sperm were fractionated by sonication into head, mid-piece and tail portions. Routine techniques of density gradient centrifugation on Percoll and/or sucrose were performed with sonicated spermatozoa for separation into their respective subfractions. The products obtained were not free of contamination in either case. Mixed sperm fractions when subjected to the afore mentioned modified capillary electrophoresis technique only the head pieces exhibited high affinity for migration towards the cathode terminal. With this method around 50% of the total sperm heads were separated and collected in absolutely pure form at the cathode side within 2 hrs. at 150 volts (V) and 1.5 milliampere (mA) current at 37.5 degrees C. A 4 cm. long capillary tube with a bore size 1.2 mm. was used for this purpose.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"24 3-4","pages":"185-92"},"PeriodicalIF":0.0,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069408010092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18831847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Purification and properties of cow splenic biliverdin reductase. 牛脾胆绿素还原酶的纯化及性质研究。

Preparative biochemistry Pub Date : 1994-11-01 DOI: 10.1080/10826069408010093

Z Ding, Y Xu

引用次数: 7

Immobilization of D-xylose (D-glucose) isomerase from a Chainia species. 链状植物d -木糖(d -葡萄糖)异构酶的固定化。

Preparative biochemistry Pub Date : 1994-05-01 DOI: 10.1080/10826069408010088

H S Pawar, D R Deshmukh

引用次数: 7

Purification and immunochemical properties of human Na,K-ATPase alpha subunits and formic acid-derived polypeptide fragments. 人Na、k - atp酶α亚基和甲酸衍生多肽片段的纯化和免疫化学性质。

Preparative biochemistry Pub Date : 1994-05-01 DOI: 10.1080/10826069408010086

J H Peng, Y Zeng, F Y Tsai, J C Parker

{"title":"Purification and immunochemical properties of human Na,K-ATPase alpha subunits and formic acid-derived polypeptide fragments.","authors":"J H Peng, Y Zeng, F Y Tsai, J C Parker","doi":"10.1080/10826069408010086","DOIUrl":"https://doi.org/10.1080/10826069408010086","url":null,"abstract":"In this study, alpha (alpha) isoform proteins were purified from the partially purified Na,K-ATPase by SDS-PAGE and electroelution. Peptide mapping showed subtle biochemical differences between alpha subunit proteins of rat and human origin. The purified alpha proteins were treated with formic acid, the cleaved polypeptide fragments were separated by SDS-PAGE, the bands corresponding to 40, 50, and 60 kDa were excised, and the proteins were electroeluted. The purified 40, 50, and 60 kDa polypeptides were essentially homogeneous, and were used for preparation of polyclonal antibodies in rabbits. The antisera to alpha proteins (R alpha) and 60 & 40 kDa polypeptides (R60 & R40) were obtained and characterized by Western blotting. All three antisera were highly specific, since they cross-reacted with only the 100 kDa bands of the crude brainstem homogenates, of the axolemma, and of the cerebral cortex synaptosomes and microsomes. R alpha and R40 were successfully used for immunohistochemical staining of fibers in the white matter of the human brain frontal cortex. These antisera were not isoform-specific, they cross-reacted with 40, 50, and 60 kDa polypeptides as well as the three alpha bands.","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"24 2","pages":"113-26"},"PeriodicalIF":0.0,"publicationDate":"1994-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069408010086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19067027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Purification of phospholipase C from rat cerebral cortex. 大鼠大脑皮层磷脂酶C的纯化。

Preparative biochemistry Pub Date : 1994-05-01 DOI: 10.1080/10826069408010085

C Y Wu, C F Chen, C F Chiang

引用次数: 1