Phytopathologia Mediterranea最新文献

Genetic variability of grapevine Pinot gris virus (GPGV) in an organically cultivated vineyard in Hungary 匈牙利有机栽培葡萄园中葡萄灰皮诺病毒（GPGV）的遗传变异性

IF 1.9 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-07-17 DOI: 10.36253/phyto-14492

R. Sáray, E. SZATHMÁRY, Dóra Pinczés, A. Almási, T. Deák, László Palkovics, K. Salánki

引用次数: 0

Diversity of Colletotrichum species on strawberry (Fragaria × ananassa) in Germany 德国草莓（Fragaria × ananassa）上 Colletotrichum 菌种的多样性

IF 1.9 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-07-17 DOI: 10.36253/phyto-15094

Christiane Rose, Ulrike Damm

{"title":"Diversity of Colletotrichum species on strawberry (Fragaria × ananassa) in Germany","authors":"Christiane Rose, Ulrike Damm","doi":"10.36253/phyto-15094","DOIUrl":"https://doi.org/10.36253/phyto-15094","url":null,"abstract":"Anthracnose caused by Colletotrichum species is an important disease of strawberries (Fragaria × ananassa), but the species causing this disease in Germany have not been investigated based on modern systematics. By using multi-locus phylogenetic analyses (ITS, act, gapdh, chs-1, his3, tub2), 58 Colletotrichum isolates from previous and recent collections, obtained mainly from fruit anthracnose of cultivated strawberries in Germany, were identified or re-identified as C. fioriniae, C. godetiae and C. nymphaeae (C. acutatum species complex) as well as C. anthrisci and C. lineola (C. dematium complex). Colletotrichum nymphaeae was dominant; most of the isolates belonged to one clonal lineage that occurs on strawberries throughout Europe, the United States of America, and some African and Asian countries. One of the other two haplotypes was distantly related and only represented by recently collected material. All other species, each of one haplotype, had only been isolated once or twice from German strawberries. This is the first report of C. anthrisci in Germany and for the genus Fragaria worldwide; all the other isolated fungi are newly reported for this genus in Germany. Comparisons of morphological characteristics of the species identified demonstrate that these features are of limited use for identification, even to species complex level. In pathogenicity tests, all five species caused anthracnose symptoms on ripe fruit of Fragaria × ananassa ‘Asia’.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141830624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing epidemiological knowledge of Botryosphaeriaceae in Mexican vineyards 加强墨西哥葡萄园中 Botryosphaeriaceae 的流行病学知识

IF 1.9 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-07-17 DOI: 10.36253/phyto-15292

E. A. RANGEL-MONTOYA, Ofelia CANDOLFI-ARBALLO, J. A. OBRADOR-SÁNCHEZ, Cesar VALENZUELA-SOLANO, R. Hernández-Martínez

{"title":"Enhancing epidemiological knowledge of Botryosphaeriaceae in Mexican vineyards","authors":"E. A. RANGEL-MONTOYA, Ofelia CANDOLFI-ARBALLO, J. A. OBRADOR-SÁNCHEZ, Cesar VALENZUELA-SOLANO, R. Hernández-Martínez","doi":"10.36253/phyto-15292","DOIUrl":"https://doi.org/10.36253/phyto-15292","url":null,"abstract":"Grapevine cultivation in Mexico is important, especially in the states of Baja California and Coahuila, which are the main wine production regions in the country. Grapevine trunk diseases (GTDs) impact productivity and cause substantial economic losses, with Botryosphaeria dieback being one of the most destructive. This disease is caused by fungi in the Botryosphaeriaceae, including species of Botryosphaeria, Diplodia, Lasiodiplodia, and Neofusicoccum. To date, Lasiodiplodia spp. are the primary Botryosphaeriaceae fungi reported in Mexico. The present study aimed to enhance the epidemiological knowledge of Botryosphaeriaceae in Mexican vineyards. Samples from grapevine plants exhibiting disease symptoms were collected from the states of Baja California and Coahuila. Of a total of 37 Botryosphaeriaceae isolates, six species were identified: Neofusicoccum parvum, N. australe, N. vitifusiforme, Botryosphaeria dothidea, Diplodia corticola, and D. seriata. Neofusicoccum parvum isolates were the most virulent, but were less virulent than previously reported Lasiodiplodia spp. The optimum growth temperatures for N. parvum and B. dothidea were from 28 to 30°C, but 25°C for D. seriata, N. vitifusiforme, and N. australe isolates. Only D. seriata isolates recovered growth when transferred to room temperature after exposure to 37°C or 40°C. This report is the first identification of B. dothidea and N. parvum as causative agents of Botryosphaeria dieback in the vine-growing regions of Mexico.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141828288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 in banana plantations in Türkiye 图尔基耶香蕉种植园中由 Fusarium oxysporum f. sp. cubense tropical race 4 引起的镰刀菌枯萎病

IF 1.9 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-07-17 DOI: 10.36253/phyto-15133

M. Özarslandan, D. S. Akgül

{"title":"Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 in banana plantations in Türkiye","authors":"M. Özarslandan, D. S. Akgül","doi":"10.36253/phyto-15133","DOIUrl":"https://doi.org/10.36253/phyto-15133","url":null,"abstract":"important disease for banana production. Presence and prevalence of FocTR4 in banana plantations on the Mediterranean coast of Türkiye were assessed during 2018 to 2020 in a total of 117 banana plantations in open fields and protected plastic greenhouses. Rhizome, pseudostem, and root samples were taken from plants showing typical symptoms associated to the disease and from suspected affected plants. Fungi were isolated from the plant internal tissues, and Fusarium oxysporum-like colonies were sub-cultured for further analyses. Phylogenetic analyses of 36 isolates showed that they belonged to four different Fusarium species: F. musae, F. oxysporum, F. sacchari, and F. solani. Eight representative F. oxysporum isolates were identified as FocTR4 by specific PCR and qPCR tests. Pathogenicity tests were carried out on tissue-cultured ‘Cavendish’ type banana seedlings (‘Grand Naine’) for 36 Fusarium isolates, and their virulence was assessed based on the internal necrosis observed in the rhizomes. Approx. 40 to 65 d after inoculations, FocTR4, F. oxysporum, and F. sacchari isolates caused severe to mild necroses in the seedling rhizomes. This is the first report of F. sacchari associated with root and collar rot of bananas in Türkiye. This study showed that Fusarium wilt caused by FocTR4 is present, but at low incidence (6.8%) in Turkish banana plantations.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141830145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and pathogenicity of Pleurostoma richardsiae causing decline of mango trees in Southern Italy 导致意大利南部芒果树衰退的 Pleurostoma richardsiae 的特征和致病性

IF 2.4 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-05-13 DOI: 10.36253/phyto-15104

G. R. Leonardi, D. Aiello, G. Gusella, G. Polizzi

引用次数: 0

Evaluation of fungicides for management of Botryosphaeriaceae associated with dieback in Australian walnut orchards 评估杀菌剂对澳大利亚核桃园中与枯萎病有关的 Botryosphaeriaceae 的防治效果

IF 2.4 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-05-13 DOI: 10.36253/phyto-14957

Stella Antony, C. C. Steel, B. Stodart, R. Billones-Baaijens, S. Savocchia

{"title":"Evaluation of fungicides for management of Botryosphaeriaceae associated with dieback in Australian walnut orchards","authors":"Stella Antony, C. C. Steel, B. Stodart, R. Billones-Baaijens, S. Savocchia","doi":"10.36253/phyto-14957","DOIUrl":"https://doi.org/10.36253/phyto-14957","url":null,"abstract":"Dieback of fruiting spurs, stems and branches of walnut trees (Juglans regia L.), caused by Botryosphaeriaceae, is widespread in walnut orchards in Australia. Five species of Botryosphaeriaceae (Diplodia seriata, Dothiorella omnivora, Neofusicoccum macroclavatum, N. parvum, and Spencermartinsia viticola) were recovered from the Australian walnut orchards in a previous study, with D. seriata and N. parvum being the most prevalent. The present study evaluated inhibitory effects of ten fungicides on mycelium growth of those five species and on conidium germination of D. seriata and N. parvum. It investigated the preventative and curative efficacy of selected fungicides on disease incidence in glasshouse and field trials. In vitro experiments showed that nine of the fungicides reduced mycelium growth, and all ten inhibited conidium germination, but to varying extents. Tebuconazole, prochloraz manganese chloride, fluazinam, fludioxonil and pyraclostrobin were the most effective for inhibiting mycelium growth (EC50 < 0.14 µg a.i. mL-1), whereas pyraclostrobin, fluxapyroxad, fluopyram, penthiopyrad and tebuconazole were the most effective for inhibiting conidium germination (EC50 < 2.2 µg a.i. mL-1). In planta experiments with five fungicides confirmed that preventative treatments had greater efficacy than curative treatments. A field trial with four commercial fungicide formulations demonstrated that tebuconazole and tebuconazole + fluopyram provided protection of walnut trees for the longest period. The field trial also confirmed the efficacy of pyraclostrobin and the inhibitory effect of fluazinam. This study is the first in Australia to evaluate fungicides in different classes and with different modes of action for efficacy against Botryosphaeriaceae recovered from walnut orchards in Australia, and provides a wider selection of active ingredients for a fungicide rotation programme than that which is currently available to the Australian walnut industry.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140982437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus velezensis B63 and chitosan control root rot, improve growth and alter the rhizosphere microbiome of geranium 枯草芽孢杆菌 B63 和壳聚糖可控制根腐病、改善天竺葵的生长并改变根瘤微生物组

IF 2.4 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-05-13 DOI: 10.36253/phyto-15093

T. R. El-sayed, N. EL-SAID, F. A. Safhi, N. E. Reyad

{"title":"Bacillus velezensis B63 and chitosan control root rot, improve growth and alter the rhizosphere microbiome of geranium","authors":"T. R. El-sayed, N. EL-SAID, F. A. Safhi, N. E. Reyad","doi":"10.36253/phyto-15093","DOIUrl":"https://doi.org/10.36253/phyto-15093","url":null,"abstract":"The root rot complex of geranium plants caused by Rhizoctonia solani and Macrophomina phaseolina is a major threat, and control of these pathogens predominantly relies on chemicals. This study explored multifaceted applications of Bacillus velezensis (strain B63) and chitosan, assessing their biocontrol efficacy against root rot, and their subsequent effects on rhizosphere communities. Strain B63 was antagonistic to R. solani and M. phaseolina. Under field conditions, greatest efficacy was obtained with strain B63 (36% and 33% disease reductions in, respectively, two growing seasons), chitosan soaking + foliar spray 0.2% (CSF 0.2%) (33 and 27% reductions), and 0.1% chitosan soaking + foliar spray (CSF 0.1%) (33 and 26% reductions). These treatments also changed rhizosphere microbiota, as shown by numbers of colony-forming units (CFU) and 16S rRNA gene microbiome analyses. Concomitant with rhizosphere shifts, essential oil yields and composition were positively affected, as shown by gas chromatography analyses. Chitosan soaking + foliar spray 0.2% increased concentrations of citronellol (1.36-fold), geraniol (1.37-fold), citronellyl formate (1.54-fold), and geranyl formate (1.94-fold) in geranium essential oil, compared with the experimental controls. Strain B63 also increased these essential oils by 1.04- to 1.27-fold. B63 also enhanced eugenol levels by 1.35-fold. Treatments with B63 were more effective than chitosan in improving the geranium plant morphological parameters (plant height, numbers of branches, biomass). These results show that B. velezensis strain B63 treatments have potential for enhancing yields and product quality from geranium plant under root rot infection.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140984354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome assemblies of several Xylella fastidiosa subspecies multiplex strains reveals high phage content and novel plasmids 几株速生木球菌亚种多重菌株的完整基因组组装揭示了高噬菌体含量和新型质粒

IF 2.4 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-02-17 DOI: 10.36253/phyto-14931

M. P. Velasco-Amo, Luis F. Arias-Giraldo, Blanca B Landa

{"title":"Complete genome assemblies of several Xylella fastidiosa subspecies multiplex strains reveals high phage content and novel plasmids","authors":"M. P. Velasco-Amo, Luis F. Arias-Giraldo, Blanca B Landa","doi":"10.36253/phyto-14931","DOIUrl":"https://doi.org/10.36253/phyto-14931","url":null,"abstract":"The Gram-negative bacterium Xylella fastidiosa (Xf) was originally found in the Americas, but has now been identified in more than 20 countries across America, Asia, and Europe. This plant pathogen is currently listed as a priority pest in Europe due to its socio-economic and ecological impacts. Within the three Xf subspecies fastidiosa, multiplex and pauca, subsp. multiplex displays a notably wider range of host plants than the other two subspecies. Comparative genomics may allow determination of how Xf subsp. multiplex adapts to new and diverse hosts and environments, so it is important that more genomes of this subspecies are defined. Twelve complete closed genomes sequences of Xf subsp. multiplex were obtained using a hybrid assembly approach combining Illumina and Oxford Nanopore technologies. The combined use of Canu and Unicycler assemblers enabled identification and closure of several plasmid sequences with high similarity to other plasmids described in strains of Xf subsp. fastidiosa and subsp. pauca. The analysis also revealed prophage sequences and contigs outside the chromosomes, annotated as phages. These new genomes, in conjunction with those existing in GenBank, will facilitate exploration of the evolutionary dynamics of Xf subsp. multiplex, its host adaptation mechanisms, and the potential emergence of novel strains of this important plant pathogen.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139959909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic analysis reveals the presence of prunus virus I in diseased Clematis vitalba: first record of this virus in Italy 元基因组分析揭示了患病铁线莲中存在梅花病毒 I：意大利首次记录到这种病毒

IF 2.4 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-02-17 DOI: 10.36253/phyto-14925

G. Parrella, E. Troiano, Anna Mignano

引用次数: 0

First report of virus detection in Ficus carica in Austria 奥地利首次报告在榕树中发现病毒

IF 2.4 3区农林科学

Phytopathologia Mediterranea Pub Date : 2024-02-17 DOI: 10.36253/phyto-14952

E. B. Borroto Fernández, T. Elbeaino, Florian FÜRNSINN, A. Keutgen, Norbert Keutgen, M. Laimer

{"title":"First report of virus detection in Ficus carica in Austria","authors":"E. B. Borroto Fernández, T. Elbeaino, Florian FÜRNSINN, A. Keutgen, Norbert Keutgen, M. Laimer","doi":"10.36253/phyto-14952","DOIUrl":"https://doi.org/10.36253/phyto-14952","url":null,"abstract":"Ficus carica is one of the most ancient cultivated crops, and is grown mainly in the Mediterranean region. In Austria, due to milder winters and longer warm periods than normal, figs are becoming more productive and popular among private growers. For future propagation of some fig varieties, the phytosanitary status of eight fig accessions, representing four Austrian genotypes maintained in a varietal collection plot, was investigated using PCR assays for presence of eight fig-infecting viruses. The four fig trees were infected with fig mosaic virus (FMV), fig badnavirus 1 (FBV-1), fig leaf mottle-associated virus 1 (FLMaV-1), fig mild mottle-associated virus (FMMaV) and fig fleck-associated virus (FFkaV); whereas fig leaf mottle-associated virus 2 (FLMaV-2), fig latent virus 1 (FLV-1) and fig cryptic virus 1 (FCV-1) were not detected. The sequences of PCR amplicons obtained from different viruses and samples showed greatest nucleotide variability of 0.5% for FBV-1, 12% for FLMaV-1, 16.3% for FMV, 14% for FMMaV, and 15% for FFkaV, when compared to their homologues in GenBank. A phylogenetic tree for FMV constructed based on partial RNA1 sequences showed that the Austrian isolates were most closely related to previously described Spanish and Greek isolates. The different symptoms observed in the tested trees were mainly in similar to with those reported for FMV, the agent of fig mosaic disease. This is the first report on the presence of fig mosaic-associated viruses in Austria.","PeriodicalId":20165,"journal":{"name":"Phytopathologia Mediterranea","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0