Planta Medica International Open最新文献_第6页

Isolation and Identification of Phenylethanoid Glycosides from Aloysia polystachya and Its Activity as Inhibitors of Monoamine Oxidase-A 多水荆苯乙醇苷的分离鉴定及其抑制单胺氧化酶a的活性

Planta Medica International Open Pub Date : 2019-01-01 DOI: 10.1055/a-0787-1665

A. Pereira, C. Guimarães, S. Pereira, E. Crevelin, G. Pinto, Lucas J. F. Morel, B. Bertoni, S. França, S. Taleb-Contini

{"title":"Isolation and Identification of Phenylethanoid Glycosides from Aloysia polystachya and Its Activity as Inhibitors of Monoamine Oxidase-A","authors":"A. Pereira, C. Guimarães, S. Pereira, E. Crevelin, G. Pinto, Lucas J. F. Morel, B. Bertoni, S. França, S. Taleb-Contini","doi":"10.1055/a-0787-1665","DOIUrl":"https://doi.org/10.1055/a-0787-1665","url":null,"abstract":"Abstract Aloysia polystachya is used as a sedative and antidepressant by the indigenous populations of Argentina and Paraguay, but the compounds associated with these activities have not been determined. We have separated and identified the main constituents of the hydroethanolic extract of A. polystachya by ultra-performance liquid chromatography-mass spectrometry and confirmed the presence of acteoside, isoacteoside, 6'-acetylacteoside, and 4’,4’’’,5,5’’-tetrahydroxy-6,6’’,3’’’-trimethoxy-[C7–O–C7’’]-biflavone by NMR spectroscopy. Inhibitory activities of the hydroethanolic extract and purified phenylethanoid glycosides against monoamine oxidase-A were assessed using a standard fluorometric assay. The hydroethanolic extract inhibited monoamine oxidase-A activity in a dose-dependent manner with an IC50 of 9.2 µg/mL, while the selective monoamine oxidase inhibitor clorgyline exhibited an IC50 of 0.06 µg/mL (0.22 µM). Acteoside was the strongest inhibitor of monoamine oxidase-A (IC50 value of 5 µM), whereas isoacteoside and 6'-acetylacteoside showed IC50 values of around 10 µM. The results showed that phenylethanoids from a hydroethanolic extract of A. polystachya have been found to have inhibitory activity against monoamine oxidase-A. It is likely that the mode of action of the acteosides is multi-targeted, involving the downregulation of inflammatory molecules and neutralization of oxidation reactions as well as inhibition of monoamine oxidase-A.","PeriodicalId":199864,"journal":{"name":"Planta Medica International Open","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130555331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Development, Validation, and Application of HPLC Method for Quantification of Antihyperuricemic Compounds from Lippia nodiflora in Rat Plasma 高效液相色谱法测定大鼠血浆中野草皮抗高尿酸血症化合物含量的建立、验证及应用

Planta Medica International Open Pub Date : 2019-01-01 DOI: 10.1055/a-0955-9505

Lee-chuen Cheng, V. Murugaiyah, K. Chan

{"title":"Development, Validation, and Application of HPLC Method for Quantification of Antihyperuricemic Compounds from Lippia nodiflora in Rat Plasma","authors":"Lee-chuen Cheng, V. Murugaiyah, K. Chan","doi":"10.1055/a-0955-9505","DOIUrl":"https://doi.org/10.1055/a-0955-9505","url":null,"abstract":"Abstract An HPLC method for simultaneous determination of arenarioside (1 ), verbascoside (2), 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5) from Lippia nodiflora in rat plasma was developed and validated. The optimal chromatographic separation was achieved with a gradient mobile phase comprising 0.1% aqueous acetic acid and acetonitrile. The limit of detection was 78.1 ng/mL for 3 and 39.1 ng/mL for the other compounds (signal-to-noise ratio=3), whereas the limit of quantification was 312.5 ng/mL for 3 and 156.3 ng/mL for the other compounds (signal-to-noise ratio=12). The recovery values of compounds 1–5 ranged from 89.37–100.92%. Their accuracy values were between 96.48 and 105.81%, while their corresponding precision values were in the range of 0.75–9.06% for both intraday and inter-day analysis. The method was then applied in the first pharmacokinetic study of 1–5. Following intravenous administration, 1–5 were eliminated slowly from the body with a mean clearance value of 0.11, 0.13, 0.30, 0.09, and 0.23 L/kg h, respectively. Meanwhile, their peak plasma concentration upon oral administration was 8.97, 1.07, 1.06, 0.65, and 0.38 µg/mL, respectively. Compound 3 (5.97%) exhibited the highest absolute oral bioavailability value, followed by 1 (5.22%), 4 (3.13%), 2 (2.10%), and 5 (0.93%).","PeriodicalId":199864,"journal":{"name":"Planta Medica International Open","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124736546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts 山茱萸果实提取物及成分对破骨细胞的影响

Planta Medica International Open Pub Date : 2019-01-01 DOI: 10.1055/a-0829-0454

M. A. E. Alves E Silva, C. Pacheco, M. Madeira, A. M. Saraiva, E. D. de Freitas, T. Valverde, J. Gomes, R. D. de Pádua, G. Kitten, Sandra Yasuyo Fukada Alves, F. Braga, T. D. da Silva

{"title":"Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts","authors":"M. A. E. Alves E Silva, C. Pacheco, M. Madeira, A. M. Saraiva, E. D. de Freitas, T. Valverde, J. Gomes, R. D. de Pádua, G. Kitten, Sandra Yasuyo Fukada Alves, F. Braga, T. D. da Silva","doi":"10.1055/a-0829-0454","DOIUrl":"https://doi.org/10.1055/a-0829-0454","url":null,"abstract":"Abstract Hancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential pharmacological applications. The present study investigated the effects of an ethanolic extract from the fruits, fractions, and compounds thereof in bone resorbing cells. Primary osteoclast cultures from bone marrow cells and osteoclasts derived from a monocyte/macrophage cell line, RAW 264.7, were incubated with different concentrations of the ethanolic extract, ethyl acetate fraction, water fraction, quinic acid, and L-(+)-bornesitol. In RAW 264.7 cell cultures, quinic acid significantly reduced osteoclast formation. In bone marrow cell-derived osteoclasts, the ethyl acetate fraction induced a decrease in the number of osteoclasts, promoting a remarkable reduction in the mean area of those cells and in their resorption activity. The compounds quinic acid and bornesitol also affected bone marrow cell-derived osteoclasts. In both cell cultures, the substances tested did not affect cell viability/proliferation. In conclusion, components extracted from H. speciosa fruit affected the cells responsible for bone resorption, making them promising tools for interference in osteoclastogenesis.","PeriodicalId":199864,"journal":{"name":"Planta Medica International Open","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129345010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Bacterial Hosts for Production of Bioactive Phenolics from Berry Fruits (BacHBerry): The BacHBerry Consortium 从浆果果实(BacHBerry)中生产生物活性酚类物质的细菌宿主:BacHBerry联盟

Planta Medica International Open Pub Date : 2018-05-01 DOI: 10.1055/S-0038-1644938

A. Dudnik

引用次数: 2

Prairie to Pharmacy Research Program: Investigation of Prairie Plants for Chemicals that Inhibit Vital Cellular Pathways 草原制药研究计划:草原植物中抑制重要细胞通路的化学物质的研究

Planta Medica International Open Pub Date : 2018-05-01 DOI: 10.1055/S-0038-1644916

A. Bosco, J. Tuescher, L. Molina, D. Tailfeathers, C. Beck, S. Kernéis, R. Andersen, RM Golsteyn

引用次数: 0

Classification of Cannabis Cultivars Marketed in Canada for Medical Purposes and Growth Trends of the North American Medical Cannabis Industry 在加拿大销售用于医疗目的的大麻品种分类和北美医用大麻产业的增长趋势

Planta Medica International Open Pub Date : 2018-05-01 DOI: 10.1055/S-0038-1644959

D. Jin, S. Jin, Y. Yu, C. Lee, J. Chen, C. Jin

引用次数: 0

Regulation of Supplemented Foods in Canada: Challenges and Evidence Gaps 加拿大对补充食品的监管:挑战和证据差距

Planta Medica International Open Pub Date : 2018-05-01 DOI: 10.1055/S-0038-1644936

J. Barber, R. Marles

引用次数: 0

Non-polar solvent fractions of Oplopanax horridus stimulate muscle glucose uptake and inhibit hepatocellular glucose-6-phosphatase enzyme activity 白芍非极性溶剂组分刺激肌肉葡萄糖摄取，抑制肝细胞葡萄糖-6-磷酸酶活性

Planta Medica International Open Pub Date : 2018-05-01 DOI: 10.1055/S-0038-1644932

M. Sánchez-Villavicencio, N. Elahmer, L. Joseph, A. Saleem, B. Hall, CS Harris, A. Cuerrier, J. Arnason, P. Haddad

引用次数: 0

Phytochemical comparison of elderberry collections grown in Missouri 密苏里州接骨木果实的植物化学比较

Planta Medica International Open Pub Date : 2018-05-01 DOI: 10.1055/S-0038-1644961

J. Dutra, EM Mudge, H. Moothoo, A. Thomas, W. Applequist, PN Brown

引用次数: 0

Evaluation of the Efficacy of Dandelion Root and Lemongrass Extracts on Prostate Cancer 蒲公英根和柠檬草提取物对前列腺癌的疗效评价

Planta Medica International Open Pub Date : 2018-05-01 DOI: 10.1055/S-0038-1644929

C. Nguyen, A. Mehaidli, S. Grewal, A. Pupulin, I. Ruvinov, K. Baskaran, S. Pandey

引用次数: 0