Pakistan Journal of Botany最新文献_第9页

Isolation and screening of thermophillic bacteria and its subsequent evaluation for lipases production 嗜热细菌的分离筛选及其对脂肪酶生产的评价

IF 1.2 4区生物学

Pakistan Journal of Botany Pub Date : 2023-09-05 DOI: 10.30848/pjb2024-2(3)

Tehmina Bashir, Hammad Majeed, T. Iftikhar

{"title":"Isolation and screening of thermophillic bacteria and its subsequent evaluation for lipases production","authors":"Tehmina Bashir, Hammad Majeed, T. Iftikhar","doi":"10.30848/pjb2024-2(3)","DOIUrl":"https://doi.org/10.30848/pjb2024-2(3)","url":null,"abstract":"Thermophilic bacteria are always being attracted for their thermostable products including enzymes. In the present work, isolation and screening of thermophilic lipolytic bacterial strains were performed from hot spring environments of Azad Jammu, Kashmir, and Manghopir, Karachi. Forty-eight bacterial strains were isolated using serial dilution method. Phenol red agar medium was used to screen hyper producers for lipases production through agar well diffusion technique. Fifteen strains were found lipolytic positive. Six were selected as lipases hyper producers based on zone hydrolysis. Their compatibility was checked with all other strains for the formulation of bacterial consortium for maximum lipolytic activity. By fermentation process, the highest lipases production was observed by consortium of Bacillus toyonensis (L2AKh) and Bacillus thuringiensis (L2AKo), which was selected for subsequent lipases synthesis. Various physiological variables, including pH, incubation temperature and incubation time, were optimized for maximum lipases synthesis. Lipases produced by thermophilic bacteria had optimum pH of 6, incubation temperature of 60 o C, and an optimum incubation period was found to be 24h. The thermostable lipases produced in the present study are suitable for exothermic reactions on an industrial scale.","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43342792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide analysis of bZIP family genes identifies their structural diversity, evolutionary patterns and expression profiles in response to salt stress in sugar beet 对 bZIP 家族基因进行全基因组分析，确定其结构多样性、进化模式以及对甜菜盐胁迫反应的表达谱

IF 1.2 4区生物学

Pakistan Journal of Botany Pub Date : 2023-09-01 DOI: 10.30848/pjb2024-2(41)

Jihen Hamdi, Narjes Kmeli, Inchirah Bettaieb, D. Bouktila

引用次数: 0

Using genetic and CRISPR/Cas9 methods to achieve quadruple mutant in trichome trimeric complex for future study in fiber initiation mechanism 利用遗传和CRISPR/Cas9方法实现毛状体三聚体的四重突变，为今后研究毛状体纤维起始机制奠定基础

4区生物学

Pakistan Journal of Botany Pub Date : 2023-09-01 DOI: 10.30848/pjb2024-2(7)

Trinh Ngoc Ai, Nghi Khac Nhu, Anh Phu Nam Bui

引用次数: 0

Physiological response and differential gene expression analysis of Hemiptelea davidii (Ulmaceae) seedlings under PEG stress PEG 胁迫下 Hemiptelea davidii（榆科）幼苗的生理反应和差异基因表达分析

IF 1.2 4区生物学

Pakistan Journal of Botany Pub Date : 2023-09-01 DOI: 10.30848/pjb2024-2(42)

Chengjun Yang, Jiayi Shen, Jingwen Shang, Tiantian Yang

引用次数: 0

Comprehensive study of the main biologically active compounds in Arctium lappa plants growing in Kazakhstan 哈萨克斯坦牛蒡植物主要生物活性成分的综合研究

4区生物学

Pakistan Journal of Botany Pub Date : 2023-09-01 DOI: 10.30848/pjb2024-2(8)

Meruyert Abdikerim, Gulbayra Azimbaeva, Bakshat Izteleu, Kenzhegul Smailova

引用次数: 0

Modulation of leaf senescence in Bergenia ciliata (Haw.) Sternb. through the supplementation of kinetin and Methyl jasmonate 毛缕草叶片衰老的调控(英文)Sternb。通过补充动素和茉莉酸甲酯

4区生物学

Pakistan Journal of Botany Pub Date : 2023-09-01 DOI: 10.30848/pjb2024-2(6)

Foziya Altaf, Shazia Parveen, Mohammad Lateef Lone, Aehsan Ul Haq, Sumira Farooq, Inayatullah Tahir, Sheikh Mansoor, Mohammad Nasser Alyemeni

引用次数: 0

Molecular mechanism of microRNA-mediated morphogenesis in plant reproductive organs: a review 植物生殖器官中microrna介导形态发生的分子机制研究进展

IF 1.2 4区生物学

Pakistan Journal of Botany Pub Date : 2023-08-27 DOI: 10.30848/pjb2024-1(35)

Xi-Ying Sun, Li Tian, Wen-Qing Jia, Lu-Ying Feng, Shu-Lin Zhang, Guang-Xin Ru

引用次数: 0

The influence of various concentrations of essential oils on the growth rate of fibrolytic and amylolytic bacteria isolated from rumen fluid 不同浓度精油对瘤胃液中分离的纤维分解菌和淀粉分解菌生长速率的影响

IF 1.2 4区生物学

Pakistan Journal of Botany Pub Date : 2023-08-27 DOI: 10.30848/pjb2024-1(36)

Zeynep Sahan

引用次数: 0

Evaluation of parameters affecting the genetic engineering of recalcitrant switchgrass (Panicum virgatum Roxb. ex Steud.) using GUS reporter gene system 影响顽固性柳枝稷基因工程的参数评价。(ex Steud.)利用GUS报告基因系统

IF 1.2 4区生物学

Pakistan Journal of Botany Pub Date : 2023-08-26 DOI: 10.30848/pjb2024-1(37)

Saima Shafique, N. Jabeen, Samra Irum

{"title":"Evaluation of parameters affecting the genetic engineering of recalcitrant switchgrass (Panicum virgatum Roxb. ex Steud.) using GUS reporter gene system","authors":"Saima Shafique, N. Jabeen, Samra Irum","doi":"10.30848/pjb2024-1(37)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(37)","url":null,"abstract":"Switchgrass (Panicum virgatum ) is used as a fodder for livestock due to its high nutritional value; it has potential to grow in cool and warm conditions as well as in low fertile soil. Switchgrass breeding is difficult due to its high degree of self-incompatibility; hence, grass production can be increased through cell and tissue culture. The present research focused on developing protocols for the regeneration and gene transformation of the forage grass to enhance the callus and regeneration efficiency through Agrobacterium -mediated transformation. The switchgrass callus was transformed with the pCAMBIA1302 vector carrying beta-glucuronidase (GUS) reporter gene, and 42% transformation efficiency was attained. To ensure a successful transformation, explants were infected with Agrobacterium for five minutes, following co-cultivation for two days. To enhance the process, 100 µM acetosyringone was added in co-cultivation media. Pre-selection of transformed callus was carried out for seven days with 150 mg/L of cefixime. The switchgrass transgenic callus was shifted to a selection medium containing hygromycin (50 mg/L). Histochemical staining was used to evaluate the presence of the GUS gene in the hygromycin-resistant transgenic plants. The successful transformation of switchgrass opens opportunities for gene function analysis and germplasm improvement through the application of advanced biotechnological tools.","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45279343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiology of Rosa ‘Gruss-an-teplitz’ following treatment with rhizobacteria (PGPR), salicylic acid (SA) and zinc sulphate 根瘤菌(PGPR)、水杨酸(SA)和硫酸锌对‘Gruss-an-teplitz’月季的生理影响

IF 1.2 4区生物学

Pakistan Journal of Botany Pub Date : 2023-08-26 DOI: 10.30848/pjb2024-1(38)

Mian. M. Amanullah, A. Bano

{"title":"Physiology of Rosa ‘Gruss-an-teplitz’ following treatment with rhizobacteria (PGPR), salicylic acid (SA) and zinc sulphate","authors":"Mian. M. Amanullah, A. Bano","doi":"10.30848/pjb2024-1(38)","DOIUrl":"https://doi.org/10.30848/pjb2024-1(38)","url":null,"abstract":"Fresh cuttings from 2-year-old rose cultivar Rose gruss-an-teplitz was treated with 2 PGPR used as seed soaking treatment and with Zinc sulphate added to the rhizosphere soil or treated with Salicylic acid (SA) as foliar spray was sown both in the field and in pots (under sterilized condition). Physiological parameters were recorded at flowering. All the treatments promoted growth parameters and improved the quality of rose water. However, greater increase in all growth parameters were recorded in Pseudomonas putida + Salicylic acid treatment. Potted grown plants also showed significant stimulation in growth and reproductive parameters; Bacillus cereus being highly effective. The treatment effects were greater in summer but in winter the % increase was also significantly greater over control. Organoleptic analysis of rose water revealed that treatment with Pseudomonas putida + Salicylic acid (SA) imparted maximum aroma and sweetness in field grown plants while Bacillus cereus inoculation proved most effective in potted plants. Plant was more responsive to treatments in summer than that of winter. Rose petal water from potted plants of winter revealed higher aroma in winter as compared to summer. Similar trend was noted in case of field grown plants. The incidence of disease was not recorded in treated plants particularly in presence of SA. Both the PGPR and SA act synergistically and can be applied to boost growth, yield and quality of roses. This is the first report regarding the effect of PGPR, defense hormone SA and Zinc sulphate alone and in combination on roses. The investigation revealed that PGPR can augment the effectivity of Zinc sulphate and SA on growth and reproductivity of rose as well as on its quality e.g., flavor and sweetness of rose water. The combined treatments may be implicated to enhance productivity and quality of roses.","PeriodicalId":19962,"journal":{"name":"Pakistan Journal of Botany","volume":" ","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47882176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0