Pharmaceutical Methods最新文献_第5页

Stability Indicating UV Spectrophotometric Method For Linagliptin and Metformin in Pharmaceutical Dosage Form 药物剂型中利格列汀、二甲双胍稳定性指示紫外分光光度法

Pharmaceutical Methods Pub Date : 2017-08-15 DOI: 10.5530/PHM.2017.8.19

Sarif Niroush Konari, Jane T. Jacob, V. Prakash

引用次数: 5

Aggrandizing Delivery of Nalidixic Acid to Colon Employing A Targeted Prodrug Approach: Synthesis and in vivo Evaluation 利用靶向前药方法强化纳利地酸到结肠:合成和体内评价

Pharmaceutical Methods Pub Date : 2017-08-15 DOI: 10.5530/PHM.2017.8.21

R. Tiwari, G. Tiwari, P. Wal, R. Dubey, A. Rai, A. Wal

{"title":"Aggrandizing Delivery of Nalidixic Acid to Colon Employing A Targeted Prodrug Approach: Synthesis and in vivo Evaluation","authors":"R. Tiwari, G. Tiwari, P. Wal, R. Dubey, A. Rai, A. Wal","doi":"10.5530/PHM.2017.8.21","DOIUrl":"https://doi.org/10.5530/PHM.2017.8.21","url":null,"abstract":"Objective: The project was aimed at synthesizing and characterizing amino acid conjugate of Nalidixic acid (NA) that is expected to enhance solubility without affecting permeability and is capable of delivering NA to colon without significant reversion of prodrug in gastrointestinal conditions. Methods: Thus, nalidixic acid-L-tryptophan conjugate (NA-TRYPh) was prepared by conventional coupling method and the prodrug was characterized by FTIR, HPLC, NMR, FAB mass and elemental analysis. The conjugate was then subjected to selected pharmaceutical preformulation studies like aqueous solubility analysis and pH partition studies. Results: These studies established 1.24 folds higher solubility of the NA-TRYPh over NA in phosphate buffer pH 7.4 without compromising its partitioning ability. The in vitro stability studies suggested its potential of safe transit to colon where the moiety is capable of reverting to 90.52% NA after 48 hrs of the experiment. In vivo evaluation of NA-TRYPh in an experimentally induced colitis established its efficacy an anti-inflammatory prodrug moiety that was supported by histological studies. In addition to its ability to control colonic ulcers NA-TRYPh demonstrated insignificant (P >0.05) gastric ulcerogenic potential. Colonic MPO activity for NA-TRYPh in mU/100 mg tissue was found to be 44.97 which were much less than plain NA (75.5). Conclusion: Conclusively, the conjugate when suitably formulated can be considered as therapeutically efficacious drug delivery system with fewer pharmaceutical limitations","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"44 1","pages":"136-143"},"PeriodicalIF":0.0,"publicationDate":"2017-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77896452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Inclusive Review on Analytical Methods for Ritonavir in Various Pharmaceutical and Biological Matrix 利托那韦在各种药物和生物基质中的分析方法综述

Pharmaceutical Methods Pub Date : 2017-08-15 DOI: 10.5530/PHM.2017.8.23

A. P. Rajput, A. P. Edlabadkar

{"title":"An Inclusive Review on Analytical Methods for Ritonavir in Various Pharmaceutical and Biological Matrix","authors":"A. P. Rajput, A. P. Edlabadkar","doi":"10.5530/PHM.2017.8.23","DOIUrl":"https://doi.org/10.5530/PHM.2017.8.23","url":null,"abstract":"Background: Ritonavir (RTV) is an anti-retroviral drug used in the treatment of HIV/ AIDS. Many times, RTV is used alone and in combination with many anti retroviral drugs. So far, around seventy nine analytical methods have been reported for various studies on analysis of RTV in bulk, pharmaceutical formulations and biological fluids. Aim: To summarize the various analytical techniques such as Chromatog raphy, Spectrophotometry; Capillary electrophoresis and also hyphenated techniques such as LCMS/MS for estimation of Ritonavir. Method: The present article deals with the comprehensive de tails about the type of various analytical techniques such as Chromatog raphy, Spectrophotometry; Capillary electrophoresis and also hyphenated techniques such as LCMS/MS, and their applicability in analysis of RTV. These techniques are either explored for the quantification, detection of metabolite and also for stability-studies of the RTV. Conclusion: The present studies re vealed that HPLC technique along with the spectroscopic have been most widely explored for the analysis. The investigatory review may provide the comprehensive details to the researchers who are working in the area of analytical research of RTV.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"99 1","pages":"149-159"},"PeriodicalIF":0.0,"publicationDate":"2017-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79296651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and Evaluation of Sustained Release Colon Targeted Micropellets of Lornoxicam 氯诺昔康结肠缓释微丸的制备与评价

Pharmaceutical Methods Pub Date : 2017-08-15 DOI: 10.5530/PHM.2017.8.12

Neetishwar Saroj, P. Rawat, Priyanka Rathour, Lokesh M. Saroj, R. Kumar

引用次数: 0

Estimation of Dapagliflozin from its Tablet Formulation by UV-Spectrophotometry 紫外分光光度法测定达格列净片剂的含量

Pharmaceutical Methods Pub Date : 2017-08-15 DOI: 10.5530/PHM.2017.8.16

G. Mante, K. Gupta, A. Hemke

引用次数: 27

Fabrication of Extended Release Tablets of Pramipexole: In-vitro Studies 普拉克索缓释片的制备:体外研究

Pharmaceutical Methods Pub Date : 2017-08-15 DOI: 10.5530/PHM.2017.8.18

K. Venkateswarlu, Heerasingh Thakur, Thumati Nagendra Bhaskar Babu

{"title":"Fabrication of Extended Release Tablets of Pramipexole: In-vitro Studies","authors":"K. Venkateswarlu, Heerasingh Thakur, Thumati Nagendra Bhaskar Babu","doi":"10.5530/PHM.2017.8.18","DOIUrl":"https://doi.org/10.5530/PHM.2017.8.18","url":null,"abstract":"In this study, Extended release (ER) tablets of Pramipexole (PMPL) to be taken once daily were prepared and evaluated. Formulations were developed using different polymers and excipients in varying concentrations to get the desired extended release period of 24 h. The granules were prepared by wet granulation method and evaluated for angle of repose (AR), bulk density (BD), tapped density (TD), Carr’s index (CI) and Hausner’s ratio (HR). The granules showed satisfactory flow properties. The compressed tablets were evaluated for weight variation, hardness, friability, drug content, thickness and in-vitro drug release. Formulation (F9) containing Sodium carboxy methyl cellulose (SCMC10%) and both grades of Micro Crystalline Cellulose (MCC PH101, MCC PH102) as diluents gave the desired release for once a day administration. The drug release was found to be followed first order kinetics and particularly diffusion with non-fickian transport mechanism. In-vitro release pattern of drug from the optimized formulation F9 was found to be similar (i.e. the similarity factor f2 was found to be 66.43) with the marketed product MIRAPEX ER and showed better drug release pattern than the marketed product. It was revealed from the results that the formulation F9 could be the suitable candidate for the effective treatment of Parkinson’s disease as once daily formulation.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"32 1","pages":"115-120"},"PeriodicalIF":0.0,"publicationDate":"2017-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84526558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Development and Validation of Five Simple UV-Spectrophotometry Methods for Estimation of Anagliptin in Bulk and in-house Tablets 五种简便紫外分光光度法测定安格列汀原料药和内服片剂含量的建立与验证

Pharmaceutical Methods Pub Date : 2016-08-01 DOI: 10.5530/PHM.2016.7.19

A. Patil, A. Shirkhedkar

引用次数: 3

Analysis of Amikacin in Human Serum By UHPLC With Fluorescence Detector Using Chloro-Formate Reagent With Glycine 甘氨酸-甲酸氯试剂UHPLC荧光检测法分析人血清中阿米卡星

Pharmaceutical Methods Pub Date : 2016-08-01 DOI: 10.5530/PHM.2016.7.15

Bindiya Chauhan, S. Jalalpure

{"title":"Analysis of Amikacin in Human Serum By UHPLC With Fluorescence Detector Using Chloro-Formate Reagent With Glycine","authors":"Bindiya Chauhan, S. Jalalpure","doi":"10.5530/PHM.2016.7.15","DOIUrl":"https://doi.org/10.5530/PHM.2016.7.15","url":null,"abstract":"Background: Amikacin belongs to aminoglycosides family, commonly administered in the treatment of systemic infections due to gram negative bacteria. Its narrow therapeutic index results in adverse effects like nephrotoxicity and ototoxicity. Objective: Optimize an ultra-high performance liquid chromatography (UHPLC) based analytical method for the determination of amikacin sulfate in human serum using derivatizaon with FMOCCl and glycine. Methods: Pre-column derivatization reaction of amikacin performed using fluorescence reagent 9-fluorenylmethyl chloroformate (FMOC-Cl) at ambient temperature in the presence of borate buffer (0.2 M). Stabilizing reagent glycine (0.1 M) added into the reaction mixture solution after completion of the derivatization reaction for stabilization of fluorescent complex product. Fluorimetric detection of amikacin was performed at excitation and emission wavelength of 265 nm and 315 nm respectively, using C18 UHPLC column. The reported method was validated by performing linearity, precision, recovery and ruggedness. Results: The optimum mobile phase composition was found to be Acetonitrile:water in the ratio of 70:30 (v/v) at flow rate of 0.4 ml/min. A linear response of amikacin in serum samples ranging from 0.5-10 μg/ml was obtained, with correlation co-efficient of 1.00. The limit of detection (LOD) was found to be 50 ng/ml. Both inter- and intra-day analysis co-efficient values were found to be less than 10%. Conclusion: The developed UHPLC method will be useful for pre-clinical and pharmacokinetic study of amikacin in human serum. Key words: Amikacin Sulphate, Ultra High Performance Liquid Chromatography (UHPLC), 9-Fluorenylmethyl chloroformate (FMOC-Cl) reagent, Borate buffer, Glycine, Fluorescence detector.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"1940 1","pages":"99-103"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91203558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Studies on Rebamipide Loaded Gastroretentive Alginate Based Mucoadhesive Beads: Formulation & In-vitro, In-vivo Evaluation 利巴米胺负载胃保留藻酸盐基黏附微球的研究:配方及体外、体内评价

Pharmaceutical Methods Pub Date : 2016-08-01 DOI: 10.5530/PHM.2016.7.20

Pooja Kashid, R. Doijad, A. Shete, Sachin Sajane, Abhimanyu Bhagat

{"title":"Studies on Rebamipide Loaded Gastroretentive Alginate Based Mucoadhesive Beads: Formulation & In-vitro, In-vivo Evaluation","authors":"Pooja Kashid, R. Doijad, A. Shete, Sachin Sajane, Abhimanyu Bhagat","doi":"10.5530/PHM.2016.7.20","DOIUrl":"https://doi.org/10.5530/PHM.2016.7.20","url":null,"abstract":"Introduction: Oral route is the most common and convenient route to deliver the drug. Many oral drug delivery systems were developed to improve drug bio availability; gastro retentive drug delivery system is one of them. Gastroretentive drug delivery system is the system in which a drug can remain in the gastric region for several hours in order to prolong its gastric residence time. The purpose of this work was to develop and evaluate rebamipide loaded gastro retentive alginate based muco adhesive beads. Materials and Methods: Rebamipide loaded alginate beads were prepared by ionotropic gelation and polyelectrolyte complexation method. Prepared beads were characterized for IR, DSC, SEM and evaluated for dissolution and stability studies. Results: The FTIR spectra revealed that there were no interaction between drug and excipients. The particle size analysis showed that beads with 3% of sodium alginate were spherical in shape. The mucoadhesion study reveals that as concentration of alginate and carbopol 934 increases percentage of mucoadhesion also increases. Conclusion: From in-vivo study it was concluded that the prepared formulation showed better control on ulcer than that of pure rebamipide. Rebamipide was successfully formulated as gastroretentive floating and mucoadhesive alginate beads by using ionotropic gelation and polyelectrolyte complexation method. Key words: Rebamipide, Gastroretentive, Alginate beads, Mucoadhesion and Carbopol 934.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"15 40 1","pages":"132-138"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79623479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Formulation Development and in-vitro Evaluation of Sulfasalazine and Dexamethasone Combination Tablets Containing Natural and Semi Synthetic Polymer for Colon Targeting 含天然和半合成聚合物的磺胺氮嗪地塞米松联合结肠靶向片的处方开发及体外评价

Pharmaceutical Methods Pub Date : 2016-08-01 DOI: 10.5530/PHM.2016.7.17

Y. Mehmood

{"title":"Formulation Development and in-vitro Evaluation of Sulfasalazine and Dexamethasone Combination Tablets Containing Natural and Semi Synthetic Polymer for Colon Targeting","authors":"Y. Mehmood","doi":"10.5530/PHM.2016.7.17","DOIUrl":"https://doi.org/10.5530/PHM.2016.7.17","url":null,"abstract":"The present research was to develop oral sustained release tablets for colon targeting. Two drugs in combination form were used in this research to prepared tablets. Combination of sulfasalazine and dexamethasone used with natural and semi synthetic polymers (tragacanth and HPMC K15). Sustained release matrix tablets of sulfasalazine and dexamethasone were prepared by using different ratios of drug, HPMC K15 and tragacanth. Microcrystalline cellulose (MCC) and lactose were used as diluents. Both polymers were mixed with other ingredients and formed a matrix system using direct compression technique. All the ingredients of formulation were compressed using concave punches in ZP 19 compression machine. Compressed tablets were evaluated for assay, diameter, hardness, thickness, friability, weight variation and in vitro dissolution using USP dissolution apparatus type II. Different formulations were prepared and evaluated with respect to dissolution profile in 900 mL 0.1 N Hcl and phosphate buffer pH 6.8 and pH7.4 including microbial flora for 12 h at 37˚C. Rising the amount of polymer (HPMC K15) in the formulation led to slow release of drug and decreasing the amount of polymer gave enhanced release of sulfasalazine and dexamethasone. Different mathematical models used to evaluate the matrix system (Zero order, First order, Higuchi and Hixson-Crowell). T5, T7, T8 solid matrix formulations followed zero order and Higuchi. The results showed that the formulation T7 containing 17% HPMC K15 and 17% gum tragacanth gives better results in microbial flora with phosphate buffer. Key words: HPMC K15, Tragacanth gum, Sulfasalazine, Dexamethasone, Sustained release.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"3 1","pages":"112-120"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76332342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1