Pharmaceutical Methods最新文献

{"title":"Pharmacognostic and Phytochemical Characteristics of the Aerial Part of Salvia moorcroftiana Wall. ex Benth. Growing Wild in Kashmir Valley, India","authors":"Riehana Gani, Z. A. Bhat, M. Dar, Zahida Shah","doi":"10.5530/PHM.2019.1.7","DOIUrl":"https://doi.org/10.5530/PHM.2019.1.7","url":null,"abstract":"Introduction: Salvia moorcroftiana Wall. ex Benth. belongs to genus Salvia, which is one of the largest genera from Lamiaceae family, which compromises about 900 species and is widely distributed in Kashmir valley. It is used medicinally in guinea worm infection, itching, colic, dysentery and boils. Objective: The present study deals with Pharmacognostic parameters of the aerial part of Salvia moorcroftiana Wall. ex Benth. Materials and Methods: The aerial part of Salvia moorcroftiana Wall. ex Benth was collected, shade dried for about 3 weeks and powdered by using mechanical grinder and the powered aerial part of plant material was evaluated for Pharmacognostic parameters by standard methods. The various fractions of Salvia moorcroftiana were subjected to preliminary phytochemical screening for the presence of various Phytoconstituents. The microscopy of the aerial part of Salvia moorcroftiana Wall. ex Benth revealed the presence of stomata, covering trichome, calcium-oxalate crystals and multicellular-headed glandular trichome. Results: Proximate analysis of the aerial part of Salvia moorcroftiana showed that the dried plant powder has 6.5 % total ash value, 1.0 % acid insoluble ash value, 0.2 % sulphated ash value. Loss on drying was found to be 5.4 %. The fractions of Salvia moorcroftiana were found to contain various phytoconstituents. Fluorescence analysis of the plant powder showed the behavour, when treated with different chemical reagents. Conclusion: The current study showed the microscopical characters, the preliminary phytochemical screening and the proximate analysis of the aerial part of Salvia moorcroftiana. Information collected from such studies can be used as benchmark in the quality control of this plant as a herbal medicine for treatment of various disease.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76954193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QSAR Studies of Triterpenoid Saponin Analogues for Nematicidal Activity","authors":"Bhushan A. Baviskar, S. Deore, P. Rathi","doi":"10.5530/PHM.2019.1.3","DOIUrl":"https://doi.org/10.5530/PHM.2019.1.3","url":null,"abstract":"2-D QSAR of triterpenoid saponin analogues with nematicidal activity performed by using three methods: Multiple Linear Regression (MLR), Partial Least Square (PLS) and Principle Component Regression (PCR). The overall degree of prediction of descriptor was found to be around 100% in all three models: MLR, PLS PCR. But, result of Multiple Linear Regression (MLR) analysis showed significant predictive power and reliability as compared to other two methods. The correlation coefficient r2-0.8684 indicates 86.84% correlation between activities and molecular descriptors of training set compound. Cross validated regression coefficient q2-0.82071 meaning that the prediction accuracy of QSAR is 82.07%. slogP descriptor having 100% positive correlation with the activity. This descriptor signifies log of the octanol/water partition coefficient (including implicit hydrogens). This property is an atomic contribution model that calculates logP from the given structure; i.e., the correct protonation state. Carboxyl group at position C-28 of aglycone is most responsible for nematicidal activity.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82012942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical Analysis of Ginger (Zingiber officinale Rosc.) Rhizome along with its TLC, HPLC and HPTLC Profile","authors":"S. S. Mian, Sukirti Upadhayay, Tajuddin, Sayyed Noman Naqvi","doi":"10.5530/PHM.2019.1.6","DOIUrl":"https://doi.org/10.5530/PHM.2019.1.6","url":null,"abstract":"Introduction: Ginger (Zingiber officinale Rosc.) is a well-known traditional medicinal plant used for therapeutic effects in both Unani and Ayurvedic System of Medicine. It has been founded to have many therapeutic activities such as anti-inflammatory, anti-rheumatic and anti-gout properties. It is also useful in the management of cough, dyspepsia, loss of appetite, relaxed sore-throat, retching, spasms / spasmodic affections of the stomach, vomiting. The present study aimed towards the evaluation of the parameters involved in the determination of the quality and purity of Zingiber officinale Rosc. rhizome and its standardization. Methods: Organoleptic characters, extractive values, ash values, phyto-chemical analysis, TLC, HPTLC, fluorescence analysis and HPLC profile etc. were the parameters used for the standardisation of the test drug. Results: Total ash values, water, alcohol and Ether soluble extractive values and volatile oil percentage was found to be 7.60%, 11.23%, 8.55%, 2.5% and 3.75% respectively. TLC profile of Z. officinale Rosc shows 05 and 13 spots in UV short and exposure in anisaldehyde-sulphuric acid respectively. The HPLC pattern shows 29 peaks and the peak no. 2, 7 and 5 are major peaks having area concentration and retention time as 9.64% at 2.66 min, 10.12% at 3.25 min. and 15.22% at 3.61 min. respectively. Conclusion: The study will provide referential information for the good quality, purity and identification for the future batches of Zingiber officinale Rosc.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78873766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and In vitro Characterization of Metronidazole Loaded Polymeric Microspheres for Colon Specific and Sustained Drug Delivery","authors":"N. Kar, Subas C. Dinda","doi":"10.5530/PHM.2019.1.1","DOIUrl":"https://doi.org/10.5530/PHM.2019.1.1","url":null,"abstract":"Objective: Metronidazole stacked alginate microspheres have been utilized to drag out the gastric living arrangement time and enhance the neighbourhood impact of medication in the district of colon for the treatment of bacterial contaminations by expecting that the colonic microbes will enzymatically corrupt the polysaccharide into natural corrosive and by bringing down the pH condition with the goal that disintegration of corrosive dissolvable covering and arrival of medication occur at the same time. Methods: Metronidazole microspheres were made by ionic gelation method utilizing Guargum, Chitosan, Eudragit S-100 and sodium alginate at various proportions and utilized calcium chloride (4% w/v) as rigidizing operator. Microspheres were described for its molecule measure, medicate stacking, tranquilize capture, swelling file and medication discharge properties. Results: Microspheres are observed to be inside size extents from 36.77μm to 229.96μm with medication capture proficiency of 42– 99% w/w. The microsphere kept up to maintain the medication discharge up to 12 h if there should be an occurrence of definition FG4. Medication discharge from the microspheres, swelling file and lightness establishes to be relies upon the grouping of chosen polymers in the polymer mixture. Definitions containing low groupings of Guargum, Chitosan, Eudragit S-100 and sodium alginate (1:1) demonstrating shorter drifting slack time and quicker medication discharge and the other way around. Accordingly, swelling record and rate of medication discharge seemed, by all accounts, to be balanced by the centralization of chose polymers in the polymer mix. The outcomes demonstrated that Glutaraldehyde (1ml) based upgraded plan FG4 could be helpful in the detailing of metronidazole microspheres for better treatment of bacterial contaminations in colon.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82971960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Zingiber officinale (Ginger) Extract on Acetaminophen- Induced Hepatotoxicity in Mice","authors":"Samia Elzwi","doi":"10.5530/PHM.2019.1.5","DOIUrl":"https://doi.org/10.5530/PHM.2019.1.5","url":null,"abstract":"Objective: To evlalate hepatoprotective effects of Zingiber officinale extract against acetaminopheninduced hepatotoxicity. Methods: B. preparation of Zingiber officinale extract. Maceration Method: In this method fresh ginger rhizome was cut into small peices, dried and then pulverized into coarse powder and weighing about 400 g of powder. It was macerated in 1000 ml hydroalcoholic solution (70%) Ethanol, 30% distilled water) for seventy two hours. The extract was then shaked, filtered by using filter paper and the solution was evaporated in a rotatory evaporator under reduced pressure until dryness. In this experiment mice of either sex weighing 25-30g were divided into three groups, each consisting of seven mice. The animals were fasted for twelve hours prior to the experiment with free access to water. 1. Control group: given normal saline containing 0.5 % Tween-80 (orally) in a dose of 1ml each mice. 2. Extract group: given hydroalcoholic extract of ginger in a dose of (300mg/kg ip) for 14 days followed by acetaminophen (300mg/kg i.p) on the 15th day from starting of the extract. 3. Acetaminophen group: given acetaminophen in single (i.p) injection of 300mg/kg. By the end of 24hr following the injection of acetaminophen, the number of death in each group was calculated, all animals were fasted for 18 hrs. before sacrifice. The collected blood used for measurement of liver transaminases and the livers were isolated, fixed in 10% formalin for histopathological analysis. Results: As present in this figure the levels of AST where 142± 8.95, 90.66±16.54, 471±80.84 unit/ ml in control, Zingiber officinale+ acetaminophen and acetaminophen treated group, whereas the level of ALT were 57.2±4.61, 31.66±9.36, 402±105.19 unit/ml in animal receiving the previously mentioned treatment respectively. By using one way ANOV and Post Hoc analysis, the level of AST and ALT was higher in acetaminophen treated group compared to the other group p≤ 0.001. Histological studies also provided evidence for the biochemical analysis . The control and Zingiber officinale treated groups showed the normal hepatocytes, portal tracts and central vein figure (A). Centrizonal necrosis accompanied by fatty changes were observed in the hepatocytes in the livers of mice in acetaminophen treated group figure (B).(C). The cellular necrosis was almost completely disappearing in the group treated with Zingiber officinale + acetaminophen groups figure (D).","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89335427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Method Validation Report for Assay of Lapatinib by UPLC","authors":"S. Biswal, S. Mondal","doi":"10.5530/PHM.2019.1.2","DOIUrl":"https://doi.org/10.5530/PHM.2019.1.2","url":null,"abstract":"Objective: A new, simple, rugged, rapid, robust and precise ultra-performance liquid chromatographic (UPLC) method for estimation of Lapatinib in a bulk and tablet dosage form has been developed and validated according to ICH Guidelines. Methods: The chromatographic separation was achieved using BHEL UPLC Column. The mobile phase used was a mixture of 0.1% OPA buffer 300 ml (30%) and 700ml Acetonitrile (70%) at isocratic mode and eluents were monitored at 309 nm using PDA detector. Results: By the method Lapatinib was eluted with retention time of 0.516 mins. The method was continued and validated accordance with ICH guidelines. Validation revealed the method is rapid, specific, accurate, precise, reliable and reproducible. Calibration curve plots were linear over the concentration ranges 10-50 μg/mL for Lapatinib. Limit of detection (LOD) were 0.06 μg/ml and limit of quantification (LOQ) were 0.18μg/mL for Lapatinib. Conclusion: The statistical analysis was proves the method is suitable for the estimation of Lapatinib as a bulk and tablet dosage form without any interference from the excipients.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87754037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Performance Thin Layer Chromatography Method Development and Validation for Simultaneous Determination of Phenolic Acids in Selected Indian Bamboo Species","authors":"Jaya Maji, Mansi M. Patel, Snehal S. Patel, Shital B Butani, P. Mehta","doi":"10.5530/PHM.2019.1.4","DOIUrl":"https://doi.org/10.5530/PHM.2019.1.4","url":null,"abstract":"","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"48 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91471469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Stability Indicating Ultra Performance Liquid Chromatography-PDA Method for the Estimation of Valganciclovir in Bulk and Tablet Dosage Form","authors":"S. Mondal, Goluguri Sunil Reddy, P. Mondal, V. Prathyusha, Aishwarya P Nair, S. Rahaman","doi":"10.5530/phm.2018.2.16","DOIUrl":"https://doi.org/10.5530/phm.2018.2.16","url":null,"abstract":"Background: The present article described about the systemic development of ultra-performance liquid chromatography (UPLC) method for the quantitative determination of valganciclovir in bulk and dosage form. The subsequent validation and degradation study was also performed. Methods: The chromatographic Separation was achieved with an HSS (100x2.1 mm, 1.8m).column with an isocratic mobile phase containing a mixture of 0.01N potassium dihydrogen orthophosphate and acetonitrile (55:45 v/v). The flow rate of the mobile phase was 0.3 ml/min with a column temperature of 30°C and detection wavelength at 254 nm. The developed method was validated according to the ICH guidelines with respect to linearity, accuracy, precision, specificity, detection limits and robustness. Results: The precision of the results, stated as the %RSD was below 1.0%. The accuracy of the method demonstrated at three levels in the range of 50%, 100% and 150% of the specification limit. The calibration curve was linear over a concentration range from 25 to 150 μg/ml with a correlation coefficient of 0.9997. The recovery of valganciclovir was found to be in the range of 98 to 102%, whereas the detection limits were found to be 0.933 and 2.827 μg/ml. Forced degradation study was carried out under acidic, alkaline, oxidative, photolytic and thermal conditions to prove the stability-indicating ability of the developed UPLC method. Conclusion: The method is validated according to the ICH guidelines and it is applied successfully for the determination of valganciclovir in tablets.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74830137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method Development and Validation for Estimation of Curcumin in Fabricated Nano-Sized Formulation: Inter-Laboratory Comparison, Capability and Statistical Analysis","authors":"S. Nadaf, S. Killedar","doi":"10.5530/phm.2018.2.11","DOIUrl":"https://doi.org/10.5530/phm.2018.2.11","url":null,"abstract":"","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74960903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"evelopment and Validation of Few UV Spectrophotometric Methods for the determination of Valganciclovir in Bulk and Pharmaceutical Dosage Form","authors":"S. Mondal, Goluguri Sunil Reddy, P. Mondal, V. Prathyusha, Aishwarya P Nair, S. Rahaman","doi":"10.5530/PHM.2018.2.12","DOIUrl":"https://doi.org/10.5530/PHM.2018.2.12","url":null,"abstract":"Background: The present research paper described about the optimization of various zero order and first order UV spectrophotometric method using different buffers. Validation study was performed to develop a simple, sensitive, rapid, accurate and economical Ultra Violet spectrophotometric method for the estimation of Valganciclovir. Methods: UV 1800 double beam UV Visible Spectrophotometer with a pair of 10mm path length matched quartz cells were used for the study. Method A (Water), Method B (phosphate buffer pH2), Method C (Phosphate buffer pH4) and Method D (phosphate buffer pH5) were developed for estimation of Valganciclovir by zero-order and first-order derivative. Results: Linearity was carried out in the concentration range of 5-60 μg/ml and the correlation coefficient were found to be 0.999. The percentage recoveries were found to be 98-102%. The relative standard deviation was found to be <2%. The LOD and LOQ were found to be 0.3241 μg/ml and 0.8227 μg/ml respectively. Conclusion: Hence, the methods were validated according to ICH guidelines and can be adopted for the routine analysis of Valganciclovir in pure and tablet dosage form.","PeriodicalId":19960,"journal":{"name":"Pharmaceutical Methods","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79186054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}