Pharmacology & toxicology最新文献_第4页

Effects of neonatal ganglioside GM1 administration on memory in adult and old rats. 新生给药神经节苷脂GM1对成年和老年大鼠记忆的影响。

Pharmacology & toxicology Pub Date : 2000-09-01 DOI: 10.1111/j.0901-9928.2000.870304.x

R H Silv, M Bergamo, R Frussa-Filho

{"title":"Effects of neonatal ganglioside GM1 administration on memory in adult and old rats.","authors":"R H Silv, M Bergamo, R Frussa-Filho","doi":"10.1111/j.0901-9928.2000.870304.x","DOIUrl":"https://doi.org/10.1111/j.0901-9928.2000.870304.x","url":null,"abstract":"Numerous investigations have been reporting the involvement of GM1 ganglioside in central nervous system development and memory formation. The effects of neonatal treatment with GMI ganglioside on the performance of adult rats in a plus-maze discriminative avoidance task and old rats in a step-down passive avoidance task were investigated. Rats were injected subcutaneously from day 3 to 15 after birth with 10 mg/kg GM1 or saline. GM1 treatment did not modify indicative landmarks of physical and motor development. Behavioural tasks were carried out when the animals were 4 (discriminative avoidance) or 24 (passive avoidance) months old. Discriminative avoidance conditioning was performed in a modified elevated plus-maze. During the training session, the animals received aversive stimulation (light and hot air blow) in one of the enclosed arms. Tests were performed 7, 14 and 21 days after conditioning (tests 1, 2 and 3), in the absence of the aversive stimulation. In all tests, GM1-treated animals spent less time in the aversive arm than in the non-aversive enclosed arm. Control animals, however, spent a shorter time in the aversive arm only in tests 1 and 2. Passive avoidance conditioning was performed in an acrylic box with a grid floor, that was partially covered by an inclined platform. Animals were placed on the platform and received a 0,5 mA foot shock when stepped down. A test was performed 48 hr later. Latency to step down presented by GM 1-treated animals was significantly higher in the test session, whereas no significant increase in latency to step down was found for control animals. The results suggest a possible action of GM1 on the maturation of the central nervous system that persists during adulthood and ageing.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 3","pages":"120-5"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21894084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of glutathione modulation on the concentration of homocysteine in plasma of rats. 谷胱甘肽调节对大鼠血浆同型半胱氨酸浓度的影响。

Pharmacology & toxicology Pub Date : 2000-09-01 DOI: 10.1111/j.0901-9928.2000.870301.x

K K Ovrebø, A Svardal

{"title":"The effect of glutathione modulation on the concentration of homocysteine in plasma of rats.","authors":"K K Ovrebø, A Svardal","doi":"10.1111/j.0901-9928.2000.870301.x","DOIUrl":"https://doi.org/10.1111/j.0901-9928.2000.870301.x","url":null,"abstract":"Elevated plasma homocysteine concentration in humans is associated with increased risk of arteriosclerosis and ischaemic heart disease. We studied whether the plasma homocysteine concentration could be changed by administration of drugs that modulate the concentration of glutathione in both plasma and tissue. Male wistar rats received reduced glutathione (0.5 mmol/kg). N-acetylcysteine (0.5 mmol/kg), L-buthionine-[S,R]-sulfoximine (2 mmol/kg) or Ringer acetate intravenously. Twenty min. later an arterial blood sample was drawn for the measurement of homocysteine and other thiols in the plasma. The thiols were quantified by reversed-phase ion-pair liquid chromatography and fluorescence detection. The total homocysteine concentration in plasma of fasted rats was 6.1+/-0.5 microM. Intravenous administration of reduced glutathione or N-acetylcysteine reduced the homocysteine concentration in plasma significantly by 51% to 3.0+/-0.3 microM and 63%, to 2.2 +/- 0.2 microM, respectively (P<0.05). In contrast, L-buthionine-[S,R]-sulfoximine increased the concentration of homocysteine by 41% to 8.6 +/- 0.6 microM (P<0.05). The glutathione concentration in plasma was 19.5 +/-1.9 microM in controls and was unchanged by N-acetylcysteine administration. Reduced glutathione increased plasma glutathione to 379.7 +/- 22.9 microM (P<0.05). whereas L-buthionine-[S R]-sulfoximine lowered the plasma glutathione concentration to 5.3 +/- 0.4 microM. Homocysteine was negatively correlated to the glutathione (r=-0.399, P<0.01) and the cysteine (r=-0.52, P<0.01) concentrations in plasma. Our conclusion is that modulation of the glutathione levels influences the concentration of homocysteine in plasma of rats.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 3","pages":"103-7"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21894215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Involvement of iron (ferric) reduction in the iron absorption mechanism of a trivalent iron-protein complex (iron protein succinylate). 三价铁蛋白复合物(琥珀酸铁蛋白)铁吸收机制中铁(铁)还原的参与。

Pharmacology & toxicology Pub Date : 2000-09-01 DOI: 10.1111/j.0901-9928.2000.870302.x

K B Raja, S E Jafri, D Dickson, A Acebròn, P Cremonesi, G Fossati, R J Simpson

{"title":"Involvement of iron (ferric) reduction in the iron absorption mechanism of a trivalent iron-protein complex (iron protein succinylate).","authors":"K B Raja, S E Jafri, D Dickson, A Acebròn, P Cremonesi, G Fossati, R J Simpson","doi":"10.1111/j.0901-9928.2000.870302.x","DOIUrl":"https://doi.org/10.1111/j.0901-9928.2000.870302.x","url":null,"abstract":"Iron protein succinylate is a non-toxic therapeutic iron compound. We set out to characterise the structure of this compound and investigate the importance of digestion and intestinal reduction in determining absorption of the compound. The structure of the compound was investigated by variable temperature Mössbauer spectroscopy, molecular size determinations and kinetics of iron release by chelators. Intestinal uptake was determined with radioactive compound force fed to mice. Reduction of the compound was determined by in vitro incubation with intestinal fragments. The compound was found to contain only ferric iron, present as small particles including sizes below 10 nm. The iron was released rapidly to chelators. Digestion with trypsin reduced the molecular size of the compound. Intestinal absorption of the compound was inhibited by a ferrous chelator (ferrozine), indicating that reduction to ferrous iron may be important for absorption. The native compound was a poor substrate for duodenal reduction activity, but digestion with pepsin, followed by pancreatin, released soluble iron complexes with an increased reduction rate. We conclude that iron protein succinylate is absorbed by a mechanism involving digestion to release soluble, available ferric species which may be reduced at the mucosal surface to provide ferrous iron for membrane transport into enterocytes.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 3","pages":"108-15"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21894082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Progesterone and testosterone modulate the convulsant actions of pentylenetetrazol and strychnine in mice. 孕酮和睾酮调节戊四唑和士的宁对小鼠的惊厥作用。

Pharmacology & toxicology Pub Date : 2000-09-01 DOI: 10.1111/j.0901-9928.2000.870303.x

M E Pesce, X Acevedo, D Bustamante, H E Miranda, G Pinardi

{"title":"Progesterone and testosterone modulate the convulsant actions of pentylenetetrazol and strychnine in mice.","authors":"M E Pesce, X Acevedo, D Bustamante, H E Miranda, G Pinardi","doi":"10.1111/j.0901-9928.2000.870303.x","DOIUrl":"https://doi.org/10.1111/j.0901-9928.2000.870303.x","url":null,"abstract":"The influence of progesterone and testosterone on the incidence of seizures after administration of intraperitoneal pentylenetetrazol and subcutaneous strychnine was evaluated in mice. Pentylenetetrazol and strychnine were administered in doses that induced seizures in 40-50% of control mice in dioestrus (48 and 0.9 mg/kg, respectively). The percentage of seizures induced by pentylenetetrazol and strychnine was significantly lower in female mice in prooestrus/oestrus, when progesterone levels are high, than in dioestrus, when progesterone levels are low. Pretreatment of pentylenetetrazol-challenged mice with progesterone (250 microg/kg) increased the incidence of seizures in prooestrus/oestrus, without affecting seizures in dioestrus. The same pretreatment in strychnine-challenged mice also increased the incidence of seizures in prooestrus-dioestrus, but significantly reduced the incidence of seizures in dioestrus. In addition, progesterone pretreatment significantly increased the percentage of deaths induced by strychnine in prooestrus-oestrus, reducing deaths in dioestrus. Orchidectomized male mice had a significantly higher incidence of seizures after administration of pentylenetetrazol and strychnine than control mice. Administration of 11 daily doses of 250 microg/kg of testosterone to castrated mice significantly reduced the incidence of seizures induced by pentylenetetrazol. These results confirm the modulatory influence of reproductive steroids on the excitability of the central nervous system and the possible clinical importance of progesterone and testosterone in the management of partial epilepsy.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 3","pages":"116-9"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21894083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Increase in blood pressure with enhanced Na+, K+ -ATPase activity in stroke-prone spontaneously hypertensive rats after 4-weeks intake of rapeseed oil as the sole dietary fat. 以菜籽油作为唯一膳食脂肪摄入4周后，卒中易发自发性高血压大鼠血压升高，Na+， K+ - atp酶活性增强。

Pharmacology & toxicology Pub Date : 2000-09-01 DOI: 10.1111/j.0901-9928.2000.870308.x

Y Naito, C Konishi, H Katsumura, N Ohara

{"title":"Increase in blood pressure with enhanced Na+, K+ -ATPase activity in stroke-prone spontaneously hypertensive rats after 4-weeks intake of rapeseed oil as the sole dietary fat.","authors":"Y Naito, C Konishi, H Katsumura, N Ohara","doi":"10.1111/j.0901-9928.2000.870308.x","DOIUrl":"https://doi.org/10.1111/j.0901-9928.2000.870308.x","url":null,"abstract":"Twenty stroke-prone spontaneously hypertensive rats were divided into 2 groups of 10 animals each, and fed a defatted diet and orally administered rapeseed (canola) oil or soybean oil at 10 (w/w)% of the consumed diet once a day for 4 weeks. At the 4th week of administration, the systolic blood pressure in the canola oil group was higher (235 +/- 2 mmHg, mean +/- S.E.M., N=10) than that in the soybean oil group (225 +/- 4 mmHg, N=10, P<0.05). In isolated, perfused mesenteric bed from these rats, the increase in perfusion pressure by norepinephrine, ATP, arachidonic acid, endothelin-1, angiotensin II or serotonin showed no between-group differences. There were also no between-group differences in the production of thromboxane A2 and prostaglandin 12 in the outflow by arachidonic acid injection. On the other hand, in the isolated aortic ring from the canola oil group, developed tension in potassium-free solution was enhanced with activation of Na+, K+ -ATPase. These results suggest that canola oil intake as the sole dietary fat increases systolic blood pressure of stroke-prone spontaneously hypertensive rats. The changes in vascular responsiveness to vasoconstrictors and production of prostanoids are unlikely to have relevance to the elevation of blood pressure. However, altered Na+, K+ -ATPase activity may play a role in the promotion of blood pressure elevation.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 3","pages":"144-8"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21894088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Copper, ceruloplasmin and superoxide dismutase (SOD) in amyotrophic lateral sclerosis. 肌萎缩性侧索硬化症中的铜、铜蓝蛋白和超氧化物歧化酶(SOD)。

Pharmacology & toxicology Pub Date : 2000-09-01 DOI: 10.1111/j.0901-9928.2000.870305.x

G Tórsdóttir, J Kristinsson, G Gudmundsson, J Snaedal, T Jóhannesson

{"title":"Copper, ceruloplasmin and superoxide dismutase (SOD) in amyotrophic lateral sclerosis.","authors":"G Tórsdóttir, J Kristinsson, G Gudmundsson, J Snaedal, T Jóhannesson","doi":"10.1111/j.0901-9928.2000.870305.x","DOIUrl":"https://doi.org/10.1111/j.0901-9928.2000.870305.x","url":null,"abstract":"In two previous studies we found copper dyshomeostasis in patients with Alzheimer's disease and in patients with Parkinson's disease. In this study, the levels of copper in plasma, of ceruloplasmin in serum, ceruloplasmin oxidative activity, ceruloplasmin specific oxidative activity (activity related to mass) as well as superoxide dismutase (SOD) activity in erythrocytes have been determined in 14 patients with amyotrophic lateral sclerosis and their healthy age- and gender-matched controls. Three of the patients had a familial form of the disease or were suspected of having it. The mean values of all parameters were found not to differ significantly between the patients and their controls (Student's t-test; P>0.05). By testing the equality of variances (F distribution) we found that the variances of individual results for ceruloplasmin specific oxidative activity and SOD activity differed significantly between the patients group and the controls group (P= 0.021 and P=0.003), but the individual results of these two activities were not correlated (P>0.05). We conclude that disturbances in ceruloplasmin specific oxidative activity and SOD activity could contribute to motor neurone death in amyotrophic lateral sclerosis, and since the two enzyme activities are not correlated it is uncertain which one is more closely related to the pathology of the disease.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 3","pages":"126-30"},"PeriodicalIF":0.0,"publicationDate":"2000-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21894085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Uptake of barbituric acid derivatives in small intestinal brush border membrane vesicles from retinyl palmitate-treated rats. 视黄醇棕榈酸处理大鼠小肠刷状膜小泡对巴比妥酸衍生物的摄取。

Pharmacology & toxicology Pub Date : 2000-08-01 DOI: 10.1034/j.1600-0773.2000.d01-48.x

H Tanii, T Horie

{"title":"Uptake of barbituric acid derivatives in small intestinal brush border membrane vesicles from retinyl palmitate-treated rats.","authors":"H Tanii, T Horie","doi":"10.1034/j.1600-0773.2000.d01-48.x","DOIUrl":"https://doi.org/10.1034/j.1600-0773.2000.d01-48.x","url":null,"abstract":"Brush border membrane was prepared from the small intestinal (jejunum) cells along the crypt-villus axis. The fluorescence spectra of 1,8-anilinonaphthalene sulfonic acid and the steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene were measured in the brush border membrane vesicle suspension. The hydrophobicity of brush border membrane was found to be in the order villus tip >mid villus >lower villus. The fluidity of brush border membrane was in the order villus tip <mid villus <lower villus. The uptake of barbituric acid derivatives by brush border membrane vesicles was well correlated with their partition coefficients (isopentyl acetate/water). No significant difference was observed between the uptake of hexobarbital by brush border membrane vesicles from the villus tip and lower villus. When retinyl palmitate was administered to rats, the fluidity of brush border membrane was found to be higher in the retinyl palmitate-treated rats than in the control rats. However, no significant difference in the uptake of hexobarbital by brush border membrane vesicles was observed between the retinyl palmitate-administered rats and the control rats. Thus, the retinyl palmitate treatment seems unlikely to affect the passively transported ligands like barbituric acid derivatives in brush border membrane vesicles.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 2","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21823258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison of in vitro and in vivo developmental toxicity and pharmacokinetics of phenytoin in the rat. 苯妥英在大鼠体内外发育毒性及药代动力学比较。

Pharmacology & toxicology Pub Date : 2000-08-01 DOI: 10.1034/j.1600-0773.2000.d01-51.x

M E Beekhuijzen, A Verhoef, R Klaassen, C J Rompelberg, A H Piersma

{"title":"Comparison of in vitro and in vivo developmental toxicity and pharmacokinetics of phenytoin in the rat.","authors":"M E Beekhuijzen, A Verhoef, R Klaassen, C J Rompelberg, A H Piersma","doi":"10.1034/j.1600-0773.2000.d01-51.x","DOIUrl":"https://doi.org/10.1034/j.1600-0773.2000.d01-51.x","url":null,"abstract":"The rat whole embryo culture was compared to an in vivo experiment with regard to embryotoxicity as well as exposure characteristics, using phenytoin as a model compound. Intra-embryonic concentrations and their embryotoxic effects were determined on gestation day 11 after in vitro administration of 50-150 microg/ml or in vivo gavage of 500-1500 mg/kg body-weight on gestation day 10. In addition, exposure kinetics were studied in vivo after a single oral dose on gestation day 10, and developmental defects on gestation day 21 were scored. The embryotoxic effects observed on gestation day 11 were more pronounced after in vitro exposure in comparison to in vivo exposure at similar intra-embryonic concentrations. Exposure of phenytoin on gestation day 10 in vitro via the culture medium resulted in general embryotoxicity on gestation day 11, whereas in vivo effects as determined on gestation day 11 were minimal. Plasma concentrations of phenytoin increased and plateaued around 35 microg/ml during the 48 hr monitoring period. Plasma concentration curves and pharmacokinetic parameters did not show remarkable differences between the dose groups, indicating that absorption is the limiting factor at the dose range used. Although the developmental effects were minimal as observed in vivo on gestation day 11, specific malformations (defects encompassing the urogenital. craniofacial and skeletal systems) were observed on gestation day 21. These findings show that with similar intra-embryonic concentrations of phenytoin the embryotoxicity in rat whole embryo culture was not comparable with the in vivo embryotoxicity as determined on gestation day 11. This discrepancy may at least partly be explained by differences in exposure characteristics.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 2","pages":"96-102"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21823261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Role of cholecystokinin receptors in induction of antinociception in hot-plate test. 热板试验中胆囊收缩素受体在抗痛性诱导中的作用。

Pharmacology & toxicology Pub Date : 2000-08-01 DOI: 10.1034/j.1600-0773.2000.d01-44.x

M Rezayat, A Rahnavard, M R Zarrindast

{"title":"Role of cholecystokinin receptors in induction of antinociception in hot-plate test.","authors":"M Rezayat, A Rahnavard, M R Zarrindast","doi":"10.1034/j.1600-0773.2000.d01-44.x","DOIUrl":"https://doi.org/10.1034/j.1600-0773.2000.d01-44.x","url":null,"abstract":"In the present study, the antinociceptive effect of cholecystokinin receptor agonists in the hot-plate test in mice has been evaluated. Subcutaneous administration of cholecystokinin octapeptide (cholecystokinin-8; 0.001, 0.005, 0.01, 0.05, and 0.1 mg/kg), unsulfated cholecystokinin octapeptide (cholecystokinin-8U; 0.1 mg/kg) or caerulein (0.25 mg/kg) produced antinociception. Administration of the cholecystokinin tetrapeptide (cholecystokinin-4; 0.25, 0.5 and 1.0 mg/kg) had no effect in the hot-plate test. Subcutaneous injection of the selective cholecystokinin receptor antagonists, MK-329 (0.125, 0.25 and 0.5 mg/kg) or L-365,260 (0.125, 0.25 and 0.5 mg/kg), produced no antinociceptive response. When the animals were pretreated with the cholecystokinin receptor antagonists or naloxone (0.5 and 1 mg/kg), a significant decrease in the antinociceptive response induced by cholecystokinin-8 and caerulein was obtained. The results indicate that single administration of cholecystokinin receptor agonists could produce an antinociceptive effect which is probably mediated via cholecystokinin receptors. With respect to the results obtained from morphine and naloxone administration, it is concluded that there may be an interaction between cholecystokinin and opiate mechanisms.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 2","pages":"58-62"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21823352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Effects of different periods of lithium pretreatment and aminoglycoside antibiotics on apomorphine-induced yawning in rats. 不同时期锂预处理及氨基糖苷类抗生素对阿吗啡致大鼠哈欠的影响。

Pharmacology & toxicology Pub Date : 2000-08-01 DOI: 10.1034/j.1600-0773.2000.d01-49.x

M Sharifzadeh, E K Firooz, M Abdollahi

{"title":"Effects of different periods of lithium pretreatment and aminoglycoside antibiotics on apomorphine-induced yawning in rats.","authors":"M Sharifzadeh, E K Firooz, M Abdollahi","doi":"10.1034/j.1600-0773.2000.d01-49.x","DOIUrl":"https://doi.org/10.1034/j.1600-0773.2000.d01-49.x","url":null,"abstract":"Interactive effects of intracerebroventricular administration of the aminoglycoside antibiotics, amikacin and gentamicin, and different duration of lithium pretreatment on apomorphine-induced yawning were investigated in male rats. The study was designed to investigate whether the hypothesis that the aminoglycoside antibiotics, amikacin and gentamicin, via their effects on phosphoinositide pathways and calcium channel might influence dopaminergic mechanisms as manifested in the yawning effect. Lithium is known to interact with phosphoinositide metabolism and was also tested after chronic studies on the apomorphine yawning model. Subcutaneous administration of apomorphine (0.1, 0.2 and 0.4 mg/kg) to rats induced yawning in a biphasic manner. However the maximum response was obtained by 0.2 mg/kg of the drug. Intracerebroventricular administration of aminoglycoside antibiotics amikacin (25 microg/rat) increased and gentamicin (10 and 20 microg/rat) decreased apomorphine-induced yawning. Pretreatment of animals with lithium (600 mg/l) in drinking water for 7, 14 and 21 days reduced yawning induced by apomorphine. Administration of lithium for 28 days did not induce any significant effect on yawning response. Amikacin and gentamicin function via the same mechanism on phosphoinositide cascade. Since amikacin and gentamicin did not affect the yawning response similarly, they apparently do not involve inositol trisphosphate level in the alterations of dopaminergic-induced yawning. Probably, the effect of lithium pretreatment on the number of yawns is also time-dependent and some tolerance to the inhibitory effect of lithium might occur after 28 days' treatment.","PeriodicalId":19876,"journal":{"name":"Pharmacology & toxicology","volume":"87 2","pages":"84-8"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21823259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2