Pflügers Archiv - European Journal of Physiology最新文献_第10页

Synergistic interplay of Gβγ and phosphatidylinositol 4,5-bisphosphate dictates Kv7.4 channel activity. Gβγ和磷脂酰肌醇4,5-二磷酸的协同相互作用决定了Kv7.4通道的活性。

Pflügers Archiv - European Journal of Physiology Pub Date : 2017-02-01 Epub Date: 2016-12-15 DOI: 10.1007/s00424-016-1916-4

Oleksandr V Povstyan, Vincenzo Barrese, Jennifer B Stott, Iain A Greenwood

{"title":"Synergistic interplay of Gβγ and phosphatidylinositol 4,5-bisphosphate dictates Kv7.4 channel activity.","authors":"Oleksandr V Povstyan, Vincenzo Barrese, Jennifer B Stott, Iain A Greenwood","doi":"10.1007/s00424-016-1916-4","DOIUrl":"10.1007/s00424-016-1916-4","url":null,"abstract":"Kv7.4 channels are key determinants of arterial contractility and cochlear mechanosensation that, like all Kv7 channels, have an obligatory requirement for phosphatidylinositol 4,5-bisphosphate (PIP2). βγ G proteins (Gβγ) have been identified as novel positive regulators of Kv7.4. The present study ascertained whether Gβγ increased Kv7.4 open probability through an increased sensitivity to PIP2. In HEK cells stably expressing Kv7.4, PIP2 or Gβγ increased open probability in a concentration dependent manner. Depleting PIP2 prevented any Gβγ-mediated stimulation whilst an array of Gβγ inhibitors prohibited any PIP2-induced current enhancement. A combination of PIP2 and Gβγ at sub-efficacious concentrations increased channel open probability considerably. The stimulatory effects of three Kv7.2-7.5 channel activators were also lost by PIP2 depletion or Gβγ inhibitors. This study alters substantially our understanding of the fundamental processes that dictate Kv7.4 activity, revealing a more complex and subtle paradigm where the reliance on local phosphoinositide is dictated by interaction with Gβγ.","PeriodicalId":19762,"journal":{"name":"Pflügers Archiv - European Journal of Physiology","volume":"9 1","pages":"213-223"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5222924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74231510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The regulation of transient receptor potential canonical 4 (TRPC4) channel by phosphodiesterase 5 inhibitor via the cyclic guanosine 3′5′-monophosphate 磷酸二酯酶5抑制剂通过环鸟苷3 ' 5 ' -单磷酸对瞬时受体电位规范4 (TRPC4)通道的调控

Pflügers Archiv - European Journal of Physiology Pub Date : 2017-01-26 DOI: 10.1007/s00424-017-1937-7

J. Wie, SeungJoo Jeong, M. Kwak, Jongyun Myeong, M. Chae, J. K. Park, S. W. Lee, I. So

引用次数: 6

Ginsenoside Rb1, a novel activator of the TMEM16A chloride channel, augments the contraction of guinea pig ileum 人参皂苷Rb1是一种新型的TMEM16A氯离子通道激活剂，可以增强豚鼠回肠的收缩

Pflügers Archiv - European Journal of Physiology Pub Date : 2017-01-25 DOI: 10.1007/s00424-017-1934-x

Shuai Guo, Yafei Chen, Chunli Pang, Xuzhao Wang, Jinlong Qi, Li Mo, Hai-Ling Zhang, Hailong An, Y. Zhan

引用次数: 37

Molecular mechanism of sarcopenia and cachexia: recent research advances 肌少症与恶病质的分子机制研究进展

Pflügers Archiv - European Journal of Physiology Pub Date : 2017-01-19 DOI: 10.1007/s00424-016-1933-3

K. Sakuma, W. Aoi, A. Yamaguchi

引用次数: 109

Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators 利用FIBSEM断层扫描和钙指标研究小鼠肾盂肾盂输尿管蠕动的间质细胞调节

Pflügers Archiv - European Journal of Physiology Pub Date : 2017-01-04 DOI: 10.1007/s00424-016-1930-6

H. Hashitani, M. Nguyen, Haruka Noda, R. Mitsui, Ryuhei Higashi, K. Ohta, Kei-ichiro Nakamura, R. Lang

引用次数: 20

Common variants in CLDN14 are associated with differential excretion of magnesium over calcium in urine. CLDN14的常见变异与尿中镁多于钙的排泄差异有关。

Pflügers Archiv - European Journal of Physiology Pub Date : 2017-01-01 Epub Date: 2016-12-03 DOI: 10.1007/s00424-016-1913-7

Tanguy Corre, Eric Olinger, Sarah E Harris, Michela Traglia, Sheila Ulivi, Stefania Lenarduzzi, Hendrica Belge, Sonia Youhanna, Natsuko Tokonami, Olivier Bonny, Pascal Houillier, Ozren Polasek, Ian J Deary, John M Starr, Daniela Toniolo, Paolo Gasparini, Peter Vollenweider, Caroline Hayward, Murielle Bochud, Olivier Devuyst

{"title":"Common variants in CLDN14 are associated with differential excretion of magnesium over calcium in urine.","authors":"Tanguy Corre, Eric Olinger, Sarah E Harris, Michela Traglia, Sheila Ulivi, Stefania Lenarduzzi, Hendrica Belge, Sonia Youhanna, Natsuko Tokonami, Olivier Bonny, Pascal Houillier, Ozren Polasek, Ian J Deary, John M Starr, Daniela Toniolo, Paolo Gasparini, Peter Vollenweider, Caroline Hayward, Murielle Bochud, Olivier Devuyst","doi":"10.1007/s00424-016-1913-7","DOIUrl":"10.1007/s00424-016-1913-7","url":null,"abstract":"The nature and importance of genetic factors regulating the differential handling of Ca2+ and Mg2+ by the renal tubule in the general population are poorly defined. We conducted a genome-wide meta-analysis of urinary magnesium-to-calcium ratio to identify associated common genetic variants. We included 9320 adults of European descent from four genetic isolates and three urban cohorts. Urinary magnesium and calcium concentrations were measured centrally in spot urine, and each study conducted linear regression analysis of urinary magnesium-to-calcium ratio on ~2.5 million single-nucleotide polymorphisms (SNPs) using an additive model. We investigated, in mouse, the renal expression profile of the top candidate gene and its variation upon changes in dietary magnesium. The genome-wide analysis evidenced a top locus (rs172639, p = 1.7 × 10-12), encompassing CLDN14, the gene coding for claudin-14, that was genome-wide significant when using urinary magnesium-to-calcium ratio, but not either one taken separately. In mouse, claudin-14 is expressed in the distal nephron segments specifically handling magnesium, and its expression is regulated by chronic changes in dietary magnesium content. A genome-wide approach identified common variants in the CLDN14 gene exerting a robust influence on the differential excretion of Mg2+ over Ca2+ in urine. These data highlight the power of urinary electrolyte ratios to unravel genetic determinants of renal tubular function. Coupled with mouse experiments, these results support a major role for claudin-14, a gene associated with kidney stones, in the differential paracellular handling of divalent cations by the renal tubule.","PeriodicalId":19762,"journal":{"name":"Pflügers Archiv - European Journal of Physiology","volume":"4 1","pages":"91-103"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77023462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcium-gated K+ channels of the KCa1.1- and KCa3.1-type couple intracellular Ca2+ signals to membrane hyperpolarization in mesenchymal stromal cells from the human adipose tissue KCa1.1-和kca3.1型的钙门控K+通道将细胞内Ca2+信号偶联到人脂肪组织间充质间质细胞的膜超极化

Pflügers Archiv - European Journal of Physiology Pub Date : 2016-12-27 DOI: 10.1007/s00424-016-1932-4

M. V. Tarasov, M. Bystrova, P. Kotova, O. A. Rogachevskaja, V. Sysoeva, S. Kolesnikov

引用次数: 7

The inhibition of voltage-gated H+ channel (HVCN1) induces acidification of leukemic Jurkat T cells promoting cell death by apoptosis 抑制电压门控H+通道(HVCN1)诱导白血病Jurkat T细胞酸化，通过凋亡促进细胞死亡

Pflügers Archiv - European Journal of Physiology Pub Date : 2016-12-24 DOI: 10.1007/s00424-016-1928-0

Agustín Asuaje, P. Smaldini, P. Martín, N. Enrique, A. Orlowski, E. A. Aiello, Carlos González Leon, G. Docena, V. Milesi

引用次数: 25

Differential distribution and functional impact of BK channel beta1 subunits across mesenteric, coronary, and different cerebral arteries of the rat BK通道β 1亚基在大鼠肠系膜、冠状动脉和不同脑动脉中的差异分布和功能影响

Pflügers Archiv - European Journal of Physiology Pub Date : 2016-12-24 DOI: 10.1007/s00424-016-1929-z

Guruprasad Kuntamallappanavar, Shivantika Bisen, A. Bukiya, Alex M. Dopico

引用次数: 1

Extracellular phosphates enhance activities of voltage-gated proton channels and production of reactive oxygen species in murine osteoclast-like cells 细胞外磷酸盐增强小鼠破骨细胞样细胞中电压门控质子通道的活性和活性氧的产生

Pflügers Archiv - European Journal of Physiology Pub Date : 2016-12-21 DOI: 10.1007/s00424-016-1931-5

Guangshuai Li, K. Miura, M. Kuno

引用次数: 6