J. Diwan, Vanitha, Shreedhara D, V. Kulkarni, K. Mahantashivayogayya, V. Ghante
{"title":"Molecular analysis of rice genotypes for fertility restoration using microsatellite markers through single marker analysis","authors":"J. Diwan, Vanitha, Shreedhara D, V. Kulkarni, K. Mahantashivayogayya, V. Ghante","doi":"10.35709/ory.2022.59.4.2","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.2","url":null,"abstract":"Genetic profiling of parental genotypes for fertility restoration is considered essential in hybrid rice technology. The traditional approach involves crossing and F1s evaluation to identify maintainer and restorer lines is cumbersome and time strategy than marker assisted selection (MAS) approach.Among various molecular markers, microsatellite markers are technically easy to use and less expensive and have extensively used for the characterization of parental lines in rice. A set of 7 SSR primers linked to rice fertility restoring genes were used for the amplification of fertility restoring alleles in 50 rice genotypes. Based on banding pattern of the 07 primers, polymorphic information content (PIC) of each primer was calculated which areranged from 0.1800 (RM 443) to 0.4928 (RM 3233 and RM 171). UPGMA and SAHN cluster analysis grouped all 50 genotypes into 15 clusters which showed a significant genetic variation among the genotypes. The single marker analysis was done with paired‘t’ test for unequal variances to find association between marker and the trait (fertility restoration). Results revealed that four out of seven SSR markers viz, RM1 and RM3233 (linked to Rf3), RM171 and RM1108 (linked to Rf4) exhibited a P-value of less than 0.05 which indicates a significant association between marker and the trait. Linked molecular markers viz., RM1, RM3233, RM171 and RM1108 can efficiently be used in identification of fertility restoring genes/alleles, facilitating the screening of rice germplasm for their fertility restoring ability. Moreover, identification of restorers through molecular markers would save time, and money adding accuracy in selection. The fully restorer lines identified in the present study can further be utilized in development of new commercial rice hybrids.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81421575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Age related histological and cooking characteristics of rice","authors":"K. Rayaguru, J. Pandey, UK Mishra, M. Mohapatra","doi":"10.35709/ory.2022.59.4.9","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.9","url":null,"abstract":"Experiments were conducted to study the effect of degree of polishing (under polished, well polished and over polished) and aging (0 to 180 days, at an interval of 30 days) on quality aspects of Pusa basmati variety of aromatic rice. An attempt has been made to investigate the histological and cooking quality changes occurring in rice, when stored for a period of six months. The cooking quality attributes considered were volume expansion ratio, kernel elongation ratio, solids loss, insoluble amylose content, alkali digestion value, gel consistency and aroma. Effect of degree of polishing on cooking quality changes was significant up to 8% of degree of polishing. With the progress of aging period, the kernel elongation ratio (24%) and volume expansion ratio (22%) increased while there was a reduction in solids loss (55%), alkali digestion score (32%) and gel consistency values (21%). The hot water insoluble fraction of amylose was found to be increased (33%) in aged rice which could be correlated with the desirable changes in cooking properties. The improvement in the cooking properties was faster during the initial three to four months of aging, thereafter the properties changed at a reduced rate. The changes were reflected more in well polished rice than that in under polished rice. A degree of milling more than 8% (over polished) did not influence these results to a great extent. The study concluded that hot water insoluble amylose content may be a major determinant of cooked rice properties as the structural changes show the increased number of large granules indicating decreased solubility.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78767808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Wahane, T. Bedse, D. Jondhale, N. Khobragade, S. Dodake
{"title":"Effect of zinc application on yield, nutrients uptake and biochemical properties of rice under Inceptisols","authors":"M. Wahane, T. Bedse, D. Jondhale, N. Khobragade, S. Dodake","doi":"10.35709/ory.2022.59.4.6","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.6","url":null,"abstract":"A field experiment was conducted in a randomized block design at Regional Agricultural Research Station, Karjat during kharif 2016-2019 to study the effect of zinc on soil fertility, yield, nutrients uptake and biochemical properties of rice under Inceptisols. Results revealed that the grain (50.65 q ha-1) and straw (56.74 q ha-1) yield of rice was significantly improved with application of zinc in the form of zinc sulphate @ 15 kg ha-1 at the time of transplanting through soil application (T7) which was better than foliar spray and root dipping methods. Significantly highest values of DTPA-extractable Zn (0.45 mg kg-1) and SO4-S (32.40 kg ha-1) were registered in the treatment T8. The zinc supplied plots led to significant enhancement in total N (93.38 kg ha-1), P (15.55 kg ha-1), K (96.80 kg ha-1) and S (35.54 kg ha-1) uptake in the treatment T7 and Zn (183.40 g ha-1) in the treatment T8. The treatment T7 showed higher hulling (74.28%), milling (63.32%), gel consistency (81mm), alkali spreading value (4.0) as well as the head rice recovery (57.80%), protein (8.96%) and amylose (24.40%) content which was acceptable.Overall, Zn-fertilization assumes great significance in improving soil properties, productivity and quality of rice.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82562949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative analysis of cooking quality and physico chemical properties of some local checks and newly developed rice varieties","authors":"Moushree Sarkar, Moumita Chatterjee, Jyotirmoy Guha","doi":"10.35709/ory.2022.59.4.8","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.8","url":null,"abstract":"Rice is predominantly consumed as a whole grain. So, the grain quality of rice is of crucial importance to determine the market price. Present experiment was conducted at our R &D farm at Bolpur during last four consecutive years of 2018, 2019, 2020, 2021. Average value of four year’s study is considered here for comparative analysis of cooking quality and physico chemical properties of our own developed 9 Open Pollinated (OP) and 10 hybrids with 19 popular OP and 6 hybrid rice varieties. Correlation study reveals significant correlation between cooking characteristic and physical properties, indicating the core relationship of physico-chemical attributes with cooking quality of the rice grain. Results also suggested that among the GMS developed lines, GMS-165B (OP), GMS-H-2515 and GMS-H-2464 were found to be the best performer recording desirable results for both grain and cooking qualities. Similarly, some lines like GMS-737, GMS-664 A and GMS-H-2499 reported to have good grain characters but some weakness in case of cooking qualities. So these lines can be used for making rice based products. The overall cooking quality and physico-chemical attributes of some of our own developed rice varieties were relatively better and / or at par as compared to other popular varieties. Our hybrids also performed as good as popular OP varieties.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"290 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76824961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Sawant, M. K. Mishra, S. Prabhukarthikeyan, A. K. Senapati, K. C. Samal
{"title":"Biochemical characterization of Bacillus species isolated from rice rhizosphere in Odisha","authors":"S. Sawant, M. K. Mishra, S. Prabhukarthikeyan, A. K. Senapati, K. C. Samal","doi":"10.35709/ory.2022.59.4.10","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.10","url":null,"abstract":"The Plant growth-promoting rhizobacteria (PGPR) such as Bacillus spp. promotes plant growth directly or indirectly. The aim of this study was to isolate the Bacillus species from rice rhizosphere and characterize by biochemical methods. A total of 10 effective Bacillus spp. were identified using both classical and biochemical identification kit methods. The HiBacillusTM kit included a number of biochemical tests viz., citrate utilisation, malonate, voges proskauer’s, ONPG, nitrate reduction, catalase, arginine, sucrose, mannitol, glucose, arabinose and trehalose. The basis for the biochemical testing is the pH change and substrate utilization. The result interpretation chart was used to identify the native bacterial isolates. The classical tests including gram’s staining, KOH testing, starch hydrolysis, IAA generation, siderophore testing, HCN testing, and proteolytic activity were carried out. Biochemical test results confirmed that ten isolates were belongs to the genus Bacillus.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87271829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of various fungitoxicants against major diseases of rice","authors":"Prasann Kumar, Chethana Bs","doi":"10.35709/ory.2022.59.4.11","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.11","url":null,"abstract":"Diseases damage to rice can seriously break productivity and with in short time destroys the crop. Screening of various groups of fungitoxicants in solo and in combination against major diseases of rice was carried out during summer and Kharif 2019. The pooled data revealed Fenoxanil 20% SC + Isoprothiolane 40% EC @ 2ml L-1 was effective in reducing the disease severity of leaf blast and neck blast by recording least percent disease index (PDI) of 13.33 and 12.22 with yield of 5099 kg ha-1as against 40.74 and 48.89 and yield 4034 kg ha-1 in control respectively. The combi fungitoxicants Azoxystrobin 25% SC + Hexaconazole 5% 16.25% SC @ 900 ml ha-1 and Kresoxim methyl 40% + Hexaconazole 8% WG @ 500 g ha-1 were effective against leaf blast, neck blast, sheath blight and sheath rot with PDI 9.25,10.40 13.33 and 17.77 respectively . The tank mix of Hexaconaxole 75% WG and Isoprothiolane 40% EC @ 66.7 g + 750 ml ha-1 was effective against leaf blast, neck blast, sheath blight and sheath rot with least PDI of 2.59, 6.33, 4.81 and 6.85 respectively. Kresoxim methyl 40% + Hexaconazole 8% in combination with Isoprothiolane 40% EC @ 500 g + 750 ml ha-1 recorded the reduced PDI of 4.81, 4.29, 11.85 and 11.11 for leaf blast, neck blast, sheath blight and sheath rot respectively .The fungitoxicants evaluated did not cause any phytotoxicity.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85864337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rice supply, demand and exportable surplus in India : Present vis-à-vis thirty years ahead","authors":"B. Mondal, J. Bisen, N. Jambhulkar, R. Tripathi","doi":"10.35709/ory.2022.59.4.13","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.13","url":null,"abstract":"The study presents a profile of rice in India and classifies different rice producing states based on productivity into high, medium and low yielding states. State wise area, production and yield (APY) data for the year 2018-19 indicates the typical concentration of rice cultivation in the eastern and southern Indian states but lowest yield in eastern states. During 1950-51 to 2018-19, rice production witnesses an average growth of 2.45 per cent per year. The study projects the future demand and supply of rice for the year 2020, 2030, 2040 and 2050 based on historical data on APY; NSSO data on per capita consumption of rice and population forecasts of the United Nations (UN) for India. Projected estimates indicates that Indian rice production may attain about 160 million tonnes by the year 2030 and 259 million tonnes during 2050. Further, the supply-demand differences for future indicates that, India would retain surplus of about 62 million tonnes of rice by the year 2050 after meeting the consumption demand of 1.65 billion population. Further, on the policy issue on dipping rice area in the country, it was also assessed that had the rice area remains the same as in 2018-19, yield level need to be increased by about 1.83 t ha-1 and if rice area decreases by 15 per cent, incremental yield to fed the burgeoning population would be around 2.62 t ha-1 during the year 2050.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81041951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Variability and association studies for yield and yield contributing traits in long grain rice (Oryza sativa L.)","authors":"Sandhya Gudepu, Damodar Raju Chennamadhavuni, Sumalini Katragadda","doi":"10.35709/ory.2022.59.4.3","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.3","url":null,"abstract":"Thirty accessions of long grain rice with grain length > 6.2mm including two checks viz., KNM 118 and IR 64 were evaluated to study variability, correlation and path analysis for yield and yield attributing traits. High range of variation, PCV and GCV were observed for number of effective tillers per plant, panicle weight, number of grains per panicle, 1000-grain weight and grain yield per plant and high heritability coupled with high genetic advance was observed for number of effective tillers per plant, plant height, panicle length, panicle weight, number of grains per panicle, 1000-grain weight, kernel length, kernel L/B ratio, grain length, grain L/B ratio and grain yield per plant. Among all the entries tested KPS 6363 and KPS 3272 were found promising with higher grain yield per plant. Correlation analysis revealed that plant height, panicle weight, grain breadth exhibited a significant and positive association with grain yield per plant. Path analysis revealed that among quantitative traits number of effective tillers per plant, plant height, panicle weight and number of grains per panicle and quality traits kernel breadth, kernel L/B ratio, grain length and kernel L/B ratio had shown positive direct effect on grain yield per plant followed by kernel breadth, plant height, panicle weight, grain length and number of grains per panicle and these traits can be used as selection indices for development of high yielding rice varieties coupled with quality.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84491670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. G, R. M, Sundaram Rm, S. M., Beulah P, N. P., M. D, Reddy Vln, Kalyani Mb, S. P, S. P
{"title":"Marker assisted backcross breeding to develop the drought tolerant version of IR58025B, a popular maintainer line of hybrid rice","authors":"K. G, R. M, Sundaram Rm, S. M., Beulah P, N. P., M. D, Reddy Vln, Kalyani Mb, S. P, S. P","doi":"10.35709/ory.2022.59.4.4","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.4","url":null,"abstract":"Drought stress has become more important abiotic factor affecting rice yield under present changing climate conditions. IR58025B is a maintainer line of widely used WA CMS line IR58025A and widely used in commercial rice hybrids production in India. Most of the rice hybrids are released for the irrigated ecosystem consequently, these derived hybrids suffer drastic yield decline under moisture stress conditions. To facilitate the efficient use of complex polygenic traits in hybrid rice molecular breeding research, we undertook development of introgression lines in background of IR58025B with tolerance to moisture stress by introgression of major QTL qDTY3.1 controlling grain yield under moisture stress. The present study reports the development and evaluation of 230 introgression lines (BC2F2) lines in IR58025B background for improvement of grain yield under moisture stress condition. Among 230 ILs, 24 introgression lines were found positive for qDTY3.1 and negative for fertility restoration (rf4 & rf3).Promising BILs exhibited earliness of 7 days under moisture stress with grain yield advantage of 8.54g to 10.78g in comparison to the recurrent parent. This suggested that the efficacy of introgressed drought tolerant QTL qDTY3.1 in enhancing grain yield under moisture stress conditions. Further, breeding lines of IR58025B possessing qDTY3.1 could also serve as good donors for development of drought tolerant rice hybrids for unfavorable ecologies.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75245806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. K. Mohapatra, R. K. Patra, A. Nayak, R. Tripathi, K. N. Mishra, L. M. Garnayak, B. S. Rath, Anjani Kumar
{"title":"Soil fertility monitoring and mapping with a minimal set of data: A case study for rice paddies","authors":"K. K. Mohapatra, R. K. Patra, A. Nayak, R. Tripathi, K. N. Mishra, L. M. Garnayak, B. S. Rath, Anjani Kumar","doi":"10.35709/ory.2022.59.4.7","DOIUrl":"https://doi.org/10.35709/ory.2022.59.4.7","url":null,"abstract":"Odisha is an agrarian state where 73% population dependent on farming and most of them are small and marginal farmer. Farmers of this state also vulnerable to different natural extreme events like flood, drought and cyclone. For sustainable crop production it is essential to develop a village level the soil fertility index (SFI) map at a higher resolution. A total of 192 gridded soil samples with a resolution of 0.5 km × 0.5 km were collected in Tangi Choudwar. The Soil organic carbon (SOC), available Nitrogen (N), Phosphorous (P), potassium (K), and micronutrient (Fe, Cu, Mn, Zn and B) maps were developed using spatial variability analysis. Then a complete soil fertility index map was developed using the Arc GIS software. In spatial variability analysis SOC and micronutrients (Fe, Cu, Mn, Zn and B) content were higher in NE site of Tangi Choudwar block. The SFI value was range between 0.21 to 0.62 with higher SFI in reported at NE site. Person’s correlation of rice grain yield showed a positively correlation with SFI (0.44, P<0.05). These results showed that soil fertility index can be used in precision agriculture during crop planning and restoration of soil fertility of a region.","PeriodicalId":19618,"journal":{"name":"ORYZA- An International Journal on Rice","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84324844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}