Oxidants and Antioxidants in Medical Science最新文献

{"title":"Therapeutic potentiality of Kedrostis foetidissima (Jacq.) Cogn., leaf extracts on free radicals induced oxidative damage in the biological system","authors":"Pavithra Kalaisezhiyen, V. Uddandrao, S. Ganapathy, V. Sasikumar","doi":"10.5455/OAMS.161216.OR.101","DOIUrl":"https://doi.org/10.5455/OAMS.161216.OR.101","url":null,"abstract":"Objectives: This study was convened to evaluate the free radical scavenging activity of Kedrostis foetidissima leaf extracts. Methods: The leaves of K. foetidissima (Jacq.) Cogn. were subjected to powder and extracted with various solvents such as water, methanol, acetone, chloroform, and petroleum ether. All the extracts were subjected to nitric oxide radical scavenging assay, hydrogen peroxide scavenging assay, superoxide anion radical scavenging assay, and egg yolk lipid peroxidation (LPO) assay. Results: All the extracts of K. foetidissima effectively reduced the generation of nitric oxide from sodium nitroprusside over a concentration of 2-10 mg/ml concentration which was significant at P < 0.05, showed moderate to potent level of H2O2 radical scavenging activity over the range of 2-10 mg/ml concentration, effectively reduced the superoxide anion radicals generated from phenazine metho-sulfate/nicotinamide adenine dinucleotide-nitroblue tetrazolium system over a concentration range of 2-10 mg/ml concentration, effectively inhibited the formation of LPO over a concentration range of 2-10 mg/ml. Conclusion: The results of this study indicated that among the five solvent extracts, the methanolic leaf extract have beneficial free radical scavenging activity and it also clearly suggested that the leaves may be used as a dietary supplement which can act as a natural antioxidant.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"15 1","pages":"14-18"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75262009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress in cigarette smokers and patients with chronic obstructive pulmonary disease","authors":"S. Taito, H. Hamada, K. Sekikawa, Norimichi Kamikawa, Makoto Takahashi","doi":"10.5455/OAMS.050417.RV.026","DOIUrl":"https://doi.org/10.5455/OAMS.050417.RV.026","url":null,"abstract":"Cigarette smoking is the most important exogenous risk factor for chronic obstructive pulmonary disease (COPD). Oxidative stress induced by smoking is considered to play major roles in the pathogenesis of COPD. In this review, we describe the theory of oxidant/antioxidant imbalance and the cellular and molecular reactions induced by oxidative stress. In addition, we discuss the effects of cigarette smoking on oxidative stress in smokers and patients with COPD. Smoking leads to increased acute pulmonary and systemic oxidative stress in cigarette smokers. As chronic effects of smoking, both pulmonary and systemic oxidative stress are increased in cigarette smokers and patients with COPD compared to non-smokers, the level of oxidative stress increases in association with duration of smoking history. Furthermore, pulmonary and systemic oxidative stress has been reported to be increased in response to exercise in cigarette smokers with a short smoking history, compared to non-smokers, although no difference was seen in baseline oxidative stress levels. Cigarette smoking affects the pulmonary and systemic oxidative stress response even in cigarette smokers with a short smoking history as well as smokers with a long smoking history and COPD patients.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"388 1","pages":"19-24"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76589237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative effect of low intensity microwave radiation in the model of developing quail embryos","authors":"O. Tsybulin, E. Sidorik, I. Yakymenko","doi":"10.5455/OAMS.210317.OR.103","DOIUrl":"https://doi.org/10.5455/OAMS.210317.OR.103","url":null,"abstract":"Objective: Exposure of humans to low intensity microwave radiation (MW) under some circumstances leads to several medical conditions, including headache, chronic fatigue, and even cancer. Mechanisms of these effects in many cases may depend on oxidative stress caused by MW exposure. Our study aims to assess oxidative stress features in embryonic cells under low intensity MW exposure in the first stages of embryogenesis. Methods: Embryos of Japanese quails were exposed in ovo to low intensity MW of GSM 900 MHz (0.25 µW/cm2) during 158-360 h discontinuously (48 c - ON, 12 c - OFF) before and in the initial stages of development. The levels of superoxide (O2·-), nitrogen oxide (NO·) and 8-oxo-2’-deoxyguanosine (8-oxo-dG) were assessed in cells of 38-h, 5- and 10-day exposed embryos and compared to the control group. Lucigenin-enhanced chemiluminescence was used for assessment of GSM modulation role in MW-induced oxidative effects. Results: A significant persistent overproduction of superoxide, nitrogen oxide and 8-oxo-dG in GSM MW exposed embryonic cells during all periods of analyses was detected. Conclusion: Exposure of developing quail embryos to low intensity MW of GSM 900 MHz during the first stages of embryogenesis resulted in a significant overproduction of superoxide and nitrogen oxide, and oxidative damages of DNA in embryonic cells. These effects were interpreted to be depended on the GSM modulation of MW.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"10 1","pages":"9-13"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87061168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multivariate discriminant analysis of redox and progression indexes in Cuban AIDS patients with Kaposi's sarcoma","authors":"L. D. Valle, Milena DuqueVizcaino, Vianka Calás-Hechavarria, Rosario Gravier-Hernández, Narciso JimenezPerez, Yusimit Bermudez-Alfonso, Daymé Hernández-Requejo, D. GonzalezRubio","doi":"10.5455/oams.210317.or.102","DOIUrl":"https://doi.org/10.5455/oams.210317.or.102","url":null,"abstract":"Objectives: Infection by human immunodeficiency virus (HIV) generates sustained reactive oxygen species production. An increasing number of studies underline the pathogenic impact of high-grade local and systemic oxidative stress in the activation of Herpes virus 8 producing Kaposi's sarcoma (KS) co-infection in acquired immunodeficiency syndrome (AIDS) patients. The present study aimed to determine the redox status in AIDS-KS Cuban individuals and to explore the relation between redox and progression variables. Methods: Blood samples were drawn from 99 individuals divided into three groups (33 each one; age range 30-50 years): AIDS, AIDS-KS and presumable healthy subjects. Total peroxide, malondialdehyde and advanced oxidation protein products as damage indexes and antioxidant responses (glutathione, peroxidation potential, superoxide dismutase and catalase) were determined from the blood samples. Also hematological and hemochemical indexes, progression indexes (viral load, CD4+ T lymphocyte absolute count) and tumoral progression indexes were assessed. Different statistical analyses were done. Results: Compared to healthy subjects, both groups of AIDS patients had significant differences in global indices of damage and antioxidant status. The comparison between the groups revealed that AIDS-KS group had a significantly higher damage and lower antioxidant status compared to the healthy control and AIDS group’s. Multivariate statistical analysis clearly separated groups according progression indexes and redox profile, obtaining two canonical functions. Conclusions: These results corroborate that substantial oxidative stress occurs in AIDS condition and also during KS-HIV co-infection with different molecular extension and interdependence to progression indexes. Redox indexes diagnosis should be considered in early diagnostics, prevention and treatment of KS-HIV co-infection, which would be worthwhile to conduct a more comprehensive study and manage of infection.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"23 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90354038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dennettia tripetala seeds inhibit ferrous sulphate-induced oxidative stress in rat tissues in vitro","authors":"F. Oyetayo, O. Ojo","doi":"10.5455/OAMS.110417.OR.104","DOIUrl":"https://doi.org/10.5455/OAMS.110417.OR.104","url":null,"abstract":"Objectives: This study was designed to investigate the antioxidant activity of pepper fruit (Dennettia tripetala) seed extract. Methods: Phenol content, reducing activity, Fe(II)-chelating ability and 1,1-diphenyl-2 picryl hydrazyl (DPPH) radical-scavenging ability of the extract were consequently examined whereas the antioxidant potentials and inhibitory effect of D.tripetala seed on FeSO4-induced oxidative stress in some rat tissues were evaluated. Results: The seed had a high concentration of total phenol. Incubation of the liver and brain tissue homogenates in the presence of Fe2+ caused a major increase in the malondialdehyde (MDA) contents of the homogenates. However, the aqueous seed extract of D.tripetala caused a significant decrease in the MDA contents of the liver and brain in a dose-dependent manner. The aqueous extract of the seed had a considerably higher inhibitory effect on Fe2+-induced lipid peroxidation in the rat brain than the liver homogenates. Conclusion: The high inhibitory effect of Dennettia tripetala seed is attributable to its high phytochemical content, Fe2+ chelating ability, DPPH scavenging ability and reducing power. These results suggest that the seed extract of D.tripetala possess body antioxidant pool improving potentials which may contribute to the prevention of the incidence of neurodegenerative maladies.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"61 1","pages":"35-39"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82785523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of antioxidant properties of 7-benzyl-8-benzylidene hydrazine xanthine derivatives in vitro and in vivo","authors":"L. Yevseyeva, M. Romanenko, K. Aleksandrova, D. Ivanchenko, S. V. Levich, S. Pavlov","doi":"10.5455/OAMS.290517.OR.107","DOIUrl":"https://doi.org/10.5455/OAMS.290517.OR.107","url":null,"abstract":"","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"96 1","pages":"41"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80565069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the in vitro anti-lipid peroxidative activity of Costus afer stem extract","authors":"Solomon A. Ndoni, D. Ere, T. Okoko","doi":"10.5455/OAMS.130417.OR.105","DOIUrl":"https://doi.org/10.5455/OAMS.130417.OR.105","url":null,"abstract":"Objective: Costus afer is a perennial monocot consumed as a remedy for various ailments. The present study investigates the effect of Costus afer stem extract on ferrous/ascorbate-induced lipid peroxidation in rat brain, liver and kidney homogenates. The inherent antioxidant and free radical scavenging potentials of the extract were also assessed using various in-vitro models. Methods: Homogenates of isolated tissues were prepared and lipid peroxidation was induced using the ferrous/ascorbate system and subsequently incubated with various concentrations of the extract. The ability of the methanolic extract to scavenge hydroxyl radicals, hydrogen peroxide and its ability to reduce ferric ions were investigated. The total antioxidant potential via the phosphomolybdate method was also measured. Results: The results revealed that Costus afer stem extract reduced tissue lipid peroxidation induced by ferrous/ascorbate. The extract also possessed significant antioxidant/free radical scavenging activity via the other in vitro models. In all cases, the effects were concentration-dependent. Conclusion: The methanolic extract of Costus afer stem possessed significant antioxidant potential ascribable to important phytochemicals and could protect cells/tissues from reactive oxygen species-induced damage. Thus the plant has high pharmacological and ethnobotanical prospects.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"14 1","pages":"30-34"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84448451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thymol and Thymus vulgaris extract protects human keratinocyte cell line (HaCaT) from UVA and UVB damage","authors":"M. Mapelli, R. Calò, L. Marabini","doi":"10.5455/OAMS.170416.OR.095","DOIUrl":"https://doi.org/10.5455/OAMS.170416.OR.095","url":null,"abstract":"Objective: The aims of our study were to characterize ultraviolet (UV)A- and UVB-induced damages in a keratinocytes cell line (HaCaT), and to evaluate the protective capacities two plant-derivative compounds, namely Thymus vulgaris L leaf extract and thymol, its major component. A polyphenol rich diet has gained wide attention and it is now considered to be a protective agent for human skin, which can be over-exposed to environmental factors and in particular UV light. Methods: Cells were pretreated for 1 h, in serum-free medium, with thymol (1 μg/ml) or Thymus vulgaris L (1.82 μg/ml) then exposed to different UVA (8-24 J/cm2) or UVB doses (0.016-0.72 J/cm2). Immediately after the UV exposure the intracellular redox status was evaluated by reactive oxygen species quantification and apoptotic events. Genotoxic aspects were evaluated 24 h after the end of irradiations using the alkaline comet assay and the immunostaining of phosphorylated H2AX histone protein (detected 1 h after the end of UV exposure). Results: The pre-treatment of our experimental model with the two substances confirmed an antioxidant action and anti-apoptotic effect by reducing the cells percentage (sub-G1 phase). Furthermore, thymol and extract of Thymus vulgaris L were able to reduce genotoxic damage. The alkaline comet assay showed that the two substances were capable to decrease DNA damage. Also in this case, Thymus vulgaris L extract is more effective than thymol in decreasing genotoxicity markers. Conclusions: Our results confirmed the more oxidant UVA and more genotoxic UVB effects. Regarding the protective effect of thymol and Thymus vulgaris L extract, data obtained proved their antioxidant and free-radical scavenging ability as known for phenolic (which our compounds belong to) and polyphenolic compounds. Thymol and mainly Thymus vulgaris L extract were also able to reduce the direct genotoxic damage.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"20 1","pages":"39-48"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89851890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic measurements of peroxynitrite scavenging properties of hydroxybenzoates","authors":"B. Veliká, B. Hubková, J. Guzy","doi":"10.5455/oams.230316.or.094","DOIUrl":"https://doi.org/10.5455/oams.230316.or.094","url":null,"abstract":"Objective: Hydroxybenzoic acids and their derivatives belongs to phenols, which are characterized by salubrious properties, leading to a reduction of the incidence of chronic diseases. The most significant health related features of hydroxybenzoic acids and their derivatives are their antioxidant properties. Peroxynitrite is a reactive nitrogen compound which can cause lipid peroxidation with subsequent cellular damage. The aim of the present work was to determine the peroxynitrite scavenging property of different hydroxybenzoates, with regard to their chemical structure. Methods: In our study, we tested 14 hydroxybenzoic acids and a number of their derivatives in the reaction with peroxynitrite. The compounds were selected to have different number and different position of the hydroxyl group(s) attached to the benzene ring. In this way we ensured the study of different suitable model compounds by testing their antioxidant properties. The peroxynitrite scavenging properties of the model compounds were studied using kinetic spectrophotometric measurements. The reaction rate was calculated based on the decrease in peroxynitrite concentration. Results: The most significant peroxynitrite scavenging properties based on the reaction rate were detected in compounds with hydroxyl groups substituted in the positions meta and para: 3,4-dihydroxybenzoic acid, gallic acid and caffeic acid. Also, increased reaction rates were recorded in the reaction of 2,4-dihydroxybenzoic acid, acetylsalicylic acid and 2-hydroxybenzoic acid with peroxynitrite. Conclusion: Our results confirmed the thesis that the free radical scavenging properties are dependent on the position and the number of the hydroxyl group(s) in relation to the carboxylic functional group attached to the benzene ring. Better understanding of the relations between the structure and the action of these antioxidants may be helpful in their proper utilization during prevention and treatment of diseases.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"77 1","pages":"28-32"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75769974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monochromatic red light of LED protects embryonic cells from oxidative stress caused by radiofrequency radiation","authors":"O. Tsybulin, E. Sidorik, S. Kyrylenko, I. Yakymenko","doi":"10.5455/oams.010216.or.092","DOIUrl":"https://doi.org/10.5455/oams.010216.or.092","url":null,"abstract":"Objective: Oxidative mechanisms of the mutagenic and carcinogenic potential of radiofrequency radiation (RFR) have been demonstrated recently. This opens the need for antioxidative approach for protection of living cells from harmful effects of RFR. In this study, we aimed to assess the antioxidant potential of monochromatic red light of light-emitting diodes (LED) in RFR-exposed embryonic cells. Methods: One group of Japanese quail embryos was exposed in ovo to GSM 900 MHz RFR (I = 1 14 μW/cm2; SAR = 0.17 mW/kg; t = 158 h; discontinuously) before and during the first hours of incubation. The second group of embryos was exposed to RFR in the same regimen and additionally to LED red light (λmax = 630-650 nm; I = 0.1 mW/cm2; t = 180 c; discontinuously). The third group of embryos were served as control. The rate of somitogenesis, level of lipid peroxidation, activity of superoxide dismutase (SOD) and catalase in tissues of 38-h embryos were assessed. Results: Red light of LED exposure resulted in statistically significant reversion of the rate of somitogenesis decreased under RFR exposure; as well as in reversion of significantly increased level of lipid peroxidation and decreased catalase activity in tissues of RFR exposed embryos. In vitro significant suppression of SOD and catalase activities by short-term RFR exposure were partially reversed by LED red light treatment. Conclusion: Red light of LED can protect embryonic cells from oxidative stress caused by low intensity RFR exposure. This is of particularly importance in terms of potential mutagenicity and carcinogenicity of low intensity RFR, which in turn depends on the oxidative potential of RFR.","PeriodicalId":19575,"journal":{"name":"Oxidants and Antioxidants in Medical Science","volume":"65 1","pages":"21-27"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83022203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}