Oncogene最新文献

{"title":"GLI1 confers resistance to PARP inhibitors by activating the DNA damage repair pathway.","authors":"Hiroshi Ikeuchi, Yusuke Matsuno, Rika Kusumoto-Matsuo, Shinya Kojima, Toshihide Ueno, Masachika Ikegami, Rina Kitada, Hitomi Sumiyoshi-Okuma, Yuki Kojima, Kan Yonemori, Yasushi Yatabe, Kazuya Takamochi, Kenji Suzuki, Ken-Ichi Yoshioka, Hiroyuki Mano, Shinji Kohsaka","doi":"10.1038/s41388-024-03105-1","DOIUrl":"10.1038/s41388-024-03105-1","url":null,"abstract":"<p><p>Identifying the mechanisms of action of anticancer drugs is an important step in the development of new drugs. In this study, we established a comprehensive screening platform consisting of 68 oncogenes (MANO panel), encompassing 243 genetic variants, to identify predictive markers for drug efficacy. Validation was performed using drugs that targeted EGFR, BRAF, and MAP2K1, which confirmed the utility of this functional screening panel. Screening of a BRCA2-knockout DLD1 cell line (DLD1-KO) revealed that cells expressing SMO and GLI1 were resistant to olaparib. Gene set enrichment analysis identified genes associated with DNA damage repair that were enriched in cells overexpressing SMO and GLI1. The expression of genes associated with homologous recombination repair (HR), such as the FANC family and BRCA1/2, was significantly upregulated by GLI1 expression, which is indicative of PARP inhibitor resistance. Although not all representative genes of the nucleotide excision repair (NER) pathway were upregulated, NER activity was enhanced by GLI1. The GLI1 inhibitor was effective against DLD1-KO cells overexpressing GLI1 both in vitro and in vivo. Furthermore, the combination therapy of olaparib and GLI1 inhibitor exhibited a synergistic effect on DLD1-KO, suggesting the possible clinical application of GLI1 inhibitor targeting cancer with defective DNA damage repair. This platform enables the identification of biomarkers associated with drug sensitivity, and is a useful tool for drug development.</p>","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Normalization of Snai1-mediated vessel dysfunction increases drug response in cancer","authors":"Helene Hoffmann,&nbsp;Martin Wartenberg,&nbsp;Sandra Vorlova,&nbsp;Franziska Karl-Schöller,&nbsp;Matthias Kallius,&nbsp;Oliver Reinhardt,&nbsp;Asli Öztürk,&nbsp;Leah S. Schuhmair,&nbsp;Verena Burkhardt,&nbsp;Sabine Gätzner,&nbsp;Daniela Scheld,&nbsp;Rajender Nandigama,&nbsp;Alma Zernecke,&nbsp;Sabine Herterich,&nbsp;Süleyman Ergün,&nbsp;Andreas Rosenwald,&nbsp;Erik Henke","doi":"10.1038/s41388-024-03113-1","DOIUrl":"10.1038/s41388-024-03113-1","url":null,"abstract":"Blood vessels in tumors are often dysfunctional. This impairs the delivery of therapeutic agents to and distribution among the cancer cells. Subsequently, treatment efficacy is reduced, and dose escalation can increase adverse effects on non-malignant tissues. The dysfunctional vessel phenotypes are attributed to aberrant pro-angiogenic signaling, and anti-angiogenic agents can ameliorate traits of vessel dysfunctionality. However, they simultaneously reduce vessel density and thereby impede drug delivery and distribution. Exploring possibilities to improve vessel functionality without compromising vessel density in the tumor microenvironment, we evaluated transcription factors (TFs) involved in epithelial-mesenchymal transition (EMT) as potential targets. Based on similarities between EMT and angiogenic activation of endothelial cells, we hypothesized that these TFs, Snai1 in particular, might serve as key regulators of vessel dysfunctionality. In vitro, experiments demonstrated that Snai1 (similarly Slug and Twist1) regulates endothelial permeability, permissiveness for tumor cell transmigration, and tip/stalk cell formation. Endothelial-specific, heterozygous knock-down of Snai1 in mice improved vascular quality in implanted tumors. This resulted in better oxygenation and reduced metastasis. Notably, the tumors in Snai1KD mice responded significantly better to chemotherapeutics as drugs were transported into the tumors at strongly increased rates and more homogeneously distributed. Thus, we demonstrate that restoring vessel homeostasis without affecting vessel density is feasible in malignant tumors. Combining such vessel re-engineering with anti-cancer drugs allows for strategic treatment approaches that reduce treatment toxicity on non-malignant tissues.","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41388-024-03113-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NNMT switches the proangiogenic phenotype of cancer-associated fibroblasts via epigenetically regulating ETS2/VEGFA axis","authors":"Xinmiao Wang,&nbsp;Hui Zhao,&nbsp;Xinyue Luo,&nbsp;Yang Chen,&nbsp;Congyu Shi,&nbsp;Yifan Wang,&nbsp;Junqiang Bai,&nbsp;Zhe Shao,&nbsp;Zhengjun Shang","doi":"10.1038/s41388-024-03112-2","DOIUrl":"10.1038/s41388-024-03112-2","url":null,"abstract":"Cancer-associated fibroblasts (CAFs) are known to promote angiogenesis in oral squamous cell carcinoma (OSCC). However, the epigenetic mechanisms through which CAFs facilitate angiogenesis within the tumor microenvironment are still poorly characterized. Nicotinamide N’-methyltransferase (NNMT), a member of the N-methyltransferase family, was found to be a key molecule in the activation of CAFs. This study shows that NNMT in fibroblasts contributes to angiogenesis and tumor growth through an epigenetic reprogramming-ETS2-VEGFA signaling axis in OSCC. Single-cell RNA Sequencing (scRNA-seq) analysis suggests that NNMT is mainly highly expressed in fibroblasts of head and neck squamous cell carcinoma (HNSCC). Moreover, analysis of the TCGA database and multiple immunohistochemical staining of clinical samples also identified a positive correlation between NNMT and tumor angiogenesis. This research further employed an assembled organoid model and a fibroblast-endothelial cell co-culture model to authenticate the proangiogenic ability of NNMT. At the molecular level, high expression of NNMT in CAFs was found to promote ETS2 expression by regulating H3K27 methylation level through mediating methylation deposition. Furthermore, ETS2 was verified to be an activating transcription factor of VEGFA in this study. Collectively, our findings delineate an epigenetic molecular regulatory network of angiogenesis and provide a theoretical basis for exploring new targets and clinical strategy in OSCC.","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic investigation of BRCA1-A, -B, and -C complexes and their functions in DNA damage response and DNA repair","authors":"Siting Li,&nbsp;Mengfan Tang,&nbsp;Yun Xiong,&nbsp;Xu Feng,&nbsp;Chao Wang,&nbsp;Litong Nie,&nbsp;Min Huang,&nbsp;Huimin Zhang,&nbsp;Ling Yin,&nbsp;Dandan Zhu,&nbsp;Chang Yang,&nbsp;Tiantian Ma,&nbsp;Junjie Chen","doi":"10.1038/s41388-024-03108-y","DOIUrl":"10.1038/s41388-024-03108-y","url":null,"abstract":"BRCA1, a breast cancer susceptibility gene, has emerged as a central mediator that brings together multiple signaling complexes in response to DNA damage. The A, B, and C complexes of BRCA1, which are formed based on their phosphorylation-dependent interactions with the BRCA1-C-terminal domains, contribute to the roles of BRCA1 in DNA repair and cell cycle checkpoint control. However, their functions in DNA damage response remain to be fully appreciated. Specifically, there has been no systematic investigation of the roles of BRCA1-A, -B, and -C complexes in the regulation of BRCA1 localization and functions, in part because of cellular lethality associated with loss of CtIP protein, which is an essential component in BRCA1-C complex. To systematically investigate the functions of these complexes in DNA damage response, we depleted a key component in each of these complexes. We used the degradation tag system to inducibly deplete endogenous CtIP and obtained a series of RAP80/FANCJ/CtIP single-, double-, and triple-knockout cells. We showed that loss of BRCA1-B/FANCJ and BRCA1-C/CtIP, but not BRCA1-A/RAP80, resulted in reduced cell proliferation and increased sensitivity to DNA damage. BRCA1-C/CtIP and BRCA1-A/RAP80 were involved in BRCA1 recruitment to sites of DNA damage. However, BRCA1-A/RAP80 was not essential for damage-induced BRCA1 localization. Instead, RAP80/H2AX and CtIP have redundant roles in BRCA1 recruitment. Altogether, our systematic analysis uncovers functional differences between BRCA1-A, -B, and -C complexes and provides new insights into the roles of these BRCA1-associated protein complexes in DNA damage response and DNA repair.","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41388-024-03108-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GATA2 promotes castration-resistant prostate cancer development by suppressing IFN-β axis-mediated antitumor immunity","authors":"Zige Jin,&nbsp;Hanling Wang,&nbsp;Ruxian Tang,&nbsp;Biying Pan,&nbsp;Hui-Ju Lee,&nbsp;Siqi Liu,&nbsp;Leiming Wang,&nbsp;Jun Qin,&nbsp;Mafei Xu","doi":"10.1038/s41388-024-03107-z","DOIUrl":"10.1038/s41388-024-03107-z","url":null,"abstract":"Castration-resistant prostate cancer (CRPC) nearly inevitably develops after long-term treatment with androgen deprivation therapy (ADT), leading to significant mortality. Investigating the mechanisms driving CRPC development is imperative. Here, we determined that the pioneer transcription factor GATA2, which is frequently amplified in CRPC patients, inhibits interferon (IFN)-β-mediated antitumor immunity, thereby promoting CRPC progression. Employing a genetically engineered mouse model (GEMM), we demonstrated that GATA2 overexpression hindered castration-induced cell apoptosis and tumor shrinkage, facilitating tumor metastasis and CRPC development. Notably, GATA2 drives castration resistance predominantly via repressing castration-induced activation of IFN-β signaling and CD8+ T-cell infiltration. This finding aligns with the negative correlation between GATA2 expression and IFNB1 expression, as well as CD8+ T-cell infiltration in CRPC patients. Mechanistically, GATA2 recruited PIAS1 as corepressor, and reprogramed the cistrome of IRF3, a key transcription factor of the IFN-β axis, in an androgen-independent manner. Furthermore, we identified a novel silencer element that facilitated the function of GATA2 and PIAS1 through looping to the IFNB1 promoter. Importantly, depletion of GATA2 augmented antitumor immunity and attenuated CRPC development. Consequently, our findings elucidate a novel mechanism wherein GATA2 promotes CRPC progression by suppressing IFN-β axis-mediated antitumor immunity, underscoring GATA2 as a promising therapeutic target for CRPC.","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41388-024-03107-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell profiling reveals altered immune landscape and impaired NK cell function in gastric cancer liver metastasis","authors":"Xiaolong Tang,&nbsp;Lei Gao,&nbsp;Xingzhi Jiang,&nbsp;Zhenyu Hou,&nbsp;Yiwen Wang,&nbsp;Shiyang Hou,&nbsp;Hui Qu","doi":"10.1038/s41388-024-03114-0","DOIUrl":"10.1038/s41388-024-03114-0","url":null,"abstract":"Gastric cancer (GC) is a substantial global health concern, and the development of liver metastasis (LM) in GC represents a critical stage linked to unfavorable patient prognoses. In this study, we employed single-cell RNA sequencing (scRNA-seq) to investigate the immune landscape of GC liver metastasis, revealing several immuno-suppressive components within the tumor immune microenvironment (TIM). Our findings unveiled an increased presence of cancer-associated fibroblasts (CAFs), myeloid-derived suppressor cell (MDSC)-like macrophages, tumor-associated macrophage (TAM)-like macrophages, and naive T cells, while conventional dendritic cells (cDCs) and effector CD8 T cells declined in LM. Additionally, we identified two distinct natural killer (NK) cell clusters exhibiting differential cytotoxicity-related gene expression, with cytotoxic NK cells notably reduced in LM. Strikingly, TGFβ was identified as an inducer of NK cell dysfunction, potentially contributing to immune evasion and tumor metastasis. In preclinical LM models, the combined approach of inhibiting TGFβ and transferring NK cells exhibited a synergistic impact, resulting in a significant reduction in liver metastasis. This work highlights the importance of understanding the complex immune dynamics within GC liver metastasis and presents a promising strategy combining TGFβ inhibition and NK-based immunotherapy to improve patient outcomes.","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Notch3-regulated microRNAs impair CXCR4-dependent maturation of thymocytes allowing maintenance and progression of T-ALL","authors":"Ilaria Sergio,&nbsp;Claudia Varricchio,&nbsp;Sandesh Kumar Patel,&nbsp;Martina Del Gaizo,&nbsp;Eleonora Russo,&nbsp;Andrea Orlando,&nbsp;Giovanna Peruzzi,&nbsp;Francesca Ferrandino,&nbsp;Georgia Tsaouli,&nbsp;Sonia Coni,&nbsp;Daniele Peluso,&nbsp;Zein Mersini Besharat,&nbsp;Federica Campolo,&nbsp;Mary Anna Venneri,&nbsp;Donatella Del Bufalo,&nbsp;Silvia Lai,&nbsp;Stefano Indraccolo,&nbsp;Sonia Minuzzo,&nbsp;Roberta La Starza,&nbsp;Giovanni Bernardini,&nbsp;Isabella Screpanti,&nbsp;Antonio Francesco Campese,&nbsp;Maria Pia Felli","doi":"10.1038/s41388-024-03111-3","DOIUrl":"10.1038/s41388-024-03111-3","url":null,"abstract":"","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41388-024-03111-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel Nav1.5-dependent feedback mechanism driving glycolytic acidification in breast cancer metastasis","authors":"Theresa K. Leslie,&nbsp;Aurelien Tripp,&nbsp;Andrew D. James,&nbsp;Scott P. Fraser,&nbsp;Michaela Nelson,&nbsp;Nattanan Sajjaboontawee,&nbsp;Alina L. Capatina,&nbsp;Michael Toss,&nbsp;Wakkas Fadhil,&nbsp;Samantha C. Salvage,&nbsp;Mar Arias Garcia,&nbsp;Melina Beykou,&nbsp;Emad Rakha,&nbsp;Valerie Speirs,&nbsp;Chris Bakal,&nbsp;George Poulogiannis,&nbsp;Mustafa B. A. Djamgoz,&nbsp;Antony P. Jackson,&nbsp;Hugh R. Matthews,&nbsp;Christopher L-H Huang,&nbsp;Andrew N. Holding,&nbsp;Sangeeta Chawla,&nbsp;William J. Brackenbury","doi":"10.1038/s41388-024-03098-x","DOIUrl":"10.1038/s41388-024-03098-x","url":null,"abstract":"Solid tumours have abnormally high intracellular [Na+]. The activity of various Na+ channels may underlie this Na+ accumulation. Voltage-gated Na+ channels (VGSCs) have been shown to be functionally active in cancer cell lines, where they promote invasion. However, the mechanisms involved, and clinical relevance, are incompletely understood. Here, we show that protein expression of the Nav1.5 VGSC subtype strongly correlates with increased metastasis and shortened cancer-specific survival in breast cancer patients. In addition, VGSCs are functionally active in patient-derived breast tumour cells, cell lines, and cancer-associated fibroblasts. Knockdown of Nav1.5 in a mouse model of breast cancer suppresses expression of invasion-regulating genes. Nav1.5 activity increases ATP demand and glycolysis in breast cancer cells, likely by upregulating activity of the Na+/K+ ATPase, thus promoting H+ production and extracellular acidification. The pH of murine xenograft tumours is lower at the periphery than in the core, in regions of higher proliferation and lower apoptosis. In turn, acidic extracellular pH elevates persistent Na+ influx through Nav1.5 into breast cancer cells. Together, these findings show positive feedback between extracellular acidification and the movement of Na+ into cancer cells which can facilitate invasion. These results highlight the clinical significance of Nav1.5 activity as a potentiator of breast cancer metastasis and provide further evidence supporting the use of VGSC inhibitors in cancer treatment.","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41388-024-03098-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Real-time imaging of TRAIL-induced apoptosis of glioma tumors in vivo","authors":"Khalid Shah,&nbsp;Yi Tang,&nbsp;Xandra Breakefield,&nbsp;Ralph Weissleder","doi":"10.1038/s41388-024-03109-x","DOIUrl":"10.1038/s41388-024-03109-x","url":null,"abstract":"","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41388-024-03109-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Expression of Concern: Generation of TRAIL-resistant cell line models reveals distinct adaptive mechanisms for acquired resistance and re-sensitization","authors":"Ahmet Cingöz,&nbsp;Ezgi Ozyerli-Goknar,&nbsp;Tunc Morova,&nbsp;Fidan Seker-Polat,&nbsp;Myvizhi Esai Selvan,&nbsp;Zeynep Hülya Gümüş,&nbsp;Deepak Bhere,&nbsp;Khalid Shah,&nbsp;Ihsan Solaroglu,&nbsp;Tugba Bagci-Onder","doi":"10.1038/s41388-024-03110-4","DOIUrl":"10.1038/s41388-024-03110-4","url":null,"abstract":"","PeriodicalId":19524,"journal":{"name":"Oncogene","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41388-024-03110-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}