Oligonucleotides最新文献

Research Progress of RNA Quadruplex. RNA Quadruplex 的研究进展。

Oligonucleotides Pub Date : 2011-05-16 DOI: 10.1089/oli.2010.0272

Xiaohui Ji, Hongxia Sun, Huaxi Zhou, Junfeng Xiang, Yalin Tang, Changqi Zhao

引用次数: 0

Polyethylenimine/oligonucleotide polyplexes investigated by fluorescence resonance energy transfer and fluorescence anisotropy. 荧光共振能量转移和荧光各向异性研究聚乙烯亚胺/寡核苷酸多聚物。

Oligonucleotides Pub Date : 2011-03-01 Epub Date: 2011-03-21 DOI: 10.1089/oli.2010.0271

Young Tag Ko, Ulrich Bickel, Juyang Huang

{"title":"Polyethylenimine/oligonucleotide polyplexes investigated by fluorescence resonance energy transfer and fluorescence anisotropy.","authors":"Young Tag Ko, Ulrich Bickel, Juyang Huang","doi":"10.1089/oli.2010.0271","DOIUrl":"https://doi.org/10.1089/oli.2010.0271","url":null,"abstract":"To advance knowledge on polyplex structure and composition, fluorescence resonance energy transfer (FRET) and anisotropy measurements were applied to polyplexes of rhodamine-labeled polyethylenimine (PEI) and fluorescein-labeled double-stranded oligodeoxynucleotide (ODN). About 25 kDa PEI was compared with low-molecular-weight PEI of 2.7 kDa. FRET reached maxima at amine to phosphate (N/P) ratios of 2 and 3 for 2.7 kDa and 25 kDa PEI, respectively, with similar average distances between donor and acceptor dye molecules in polyplexes. Anisotropy measurements allowed estimating the bound fractions of PEI and ODN. At N/P = 6, all ODN was bound, but only 58% of PEI 25 kDa and 45% of PEI 2.7 kDa. In conclusion, the higher molecular weight of PEI may conformationally restrict the availability of amino groups for charge interaction with phosphate groups in ODN. Moreover, significant fractions of both types of PEI remain free in solution at N/P ratios frequently used for transfection. FRET and anisotropy measurements provide effective tools for probing polyplex compositions and designing optimized delivery systems.","PeriodicalId":19523,"journal":{"name":"Oligonucleotides","volume":"21 2","pages":"109-14"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/oli.2010.0271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29752450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Decoy-DNA against special AT-rich sequence binding protein 1 inhibits the growth and invasive ability of human breast cancer. 针对特殊AT-rich序列结合蛋白1的诱饵dna抑制人乳腺癌的生长和侵袭能力。

Oligonucleotides Pub Date : 2011-03-01 DOI: 10.1089/oli.2010.0277

Asako Yamayoshi, Mariko Yasuhara, Sanjeev Galande, Akio Kobori, Akira Murakami

{"title":"Decoy-DNA against special AT-rich sequence binding protein 1 inhibits the growth and invasive ability of human breast cancer.","authors":"Asako Yamayoshi, Mariko Yasuhara, Sanjeev Galande, Akio Kobori, Akira Murakami","doi":"10.1089/oli.2010.0277","DOIUrl":"https://doi.org/10.1089/oli.2010.0277","url":null,"abstract":"\"Triple-negative\" (TN) breast cancers, which are characterized by estrogen receptor (-), progesterone receptor (-), and human epidermal growth factor receptor 2 (-), are typically associated with poor prognosis because of their aggressive tumor phenotypes. In recent years, the number of patients with breast cancers has remarkably increased, but there are only few available drugs for treatment of TN breast cancers. The development of novel drugs targeting TN breast cancer is urgently required. In the present study, we focused on the function of special AT-rich sequence binding protein 1 (SATB1) as a target molecule for the treatment of TN breast cancers. By recruiting chromatin remodeling enzymes and transcriptional factors, SATB1 regulates the expression of >1,000 genes related to cell growth and translocation. We synthesized a decoy DNA against SATB1, including the recognition sequence of SATB1. We examined the inhibitory effects of the decoy DNAs on cellular proliferation of a TN metastatic breast cancer cell line (MDA-MB-231). SATB1-decoy DNA inhibited the proliferation of MDA-MB-231 cells. Especially, it was significant that SATB1-decoy DNA drastically reduced the invasive and metastatic capacity of MBA-MB-231 cells. Further, in the case of MCF7 cells (SATB1-negative breast cancer cell line), SATB1-decoy DNA did not exhibit any inhibitory effect. These data suggest that SATB1-decoy DNA may be an effective candidate for use as a molecular-targeting drug for treatment of TN breast cancer.","PeriodicalId":19523,"journal":{"name":"Oligonucleotides","volume":"21 2","pages":"115-21"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/oli.2010.0277","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29823042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

PEI-g-PEG-RGD/small interference RNA polyplex-mediated silencing of vascular endothelial growth factor receptor and its potential as an anti-angiogenic tumor therapeutic strategy. PEI-g-PEG-RGD/小干扰RNA多聚体介导的血管内皮生长因子受体沉默及其作为抗血管生成肿瘤治疗策略的潜力。

Oligonucleotides Pub Date : 2011-03-01 Epub Date: 2011-03-04 DOI: 10.1089/oli.2011.0278

Jihoon Kim, Sung Wan Kim, Won Jong Kim

{"title":"PEI-g-PEG-RGD/small interference RNA polyplex-mediated silencing of vascular endothelial growth factor receptor and its potential as an anti-angiogenic tumor therapeutic strategy.","authors":"Jihoon Kim, Sung Wan Kim, Won Jong Kim","doi":"10.1089/oli.2011.0278","DOIUrl":"https://doi.org/10.1089/oli.2011.0278","url":null,"abstract":"Tumor angiogenesis appears to be achieved by the expression of vascular endothelial growth factor (VEGF) within solid tumors that stimulate host vascular endothelial cell mitogenesis and possibly chemotaxis. VEGF's angiogenic actions are mediated through its high-affinity binding to 2 endothelium-specific receptor tyrosine kinase, Flt-1 (VEGFR1), and Flk-1/KDR (VEGFR2). RNA interference-mediated knockdown of protein expression at the messenger RNA level provides a new therapeutic strategy to overcome various diseases. To achieve high efficacy in RNA interference-mediated therapy, it is critical to develop an efficient delivering system to deliver small interference RNA (siRNA) into tissues or cells site-specifically. We previously reported an angiogenic endothelial cell-targeted polymeric gene carrier, PEI-g-PEG-RGD. This targeted carrier was developed by the conjugation of the ανβ3/ανβ5 integrin-binding RGD peptide (ACDCRGDCFC) to the cationic polymer, branched polyethylenimine, with a hydrophilic polyethylene glycol (PEG) spacer. In this study, we used PEI-g-PEG-RGD to deliver siRNA against VEGFR1 into tumor site. The physicochemical properties of PEI-g-PEG-RGD/siRNA complexes was evaluated. Further, tumor growth profile was also investigated after systemic administration of PEI-g-PEG-RGD/siRNA complexes.","PeriodicalId":19523,"journal":{"name":"Oligonucleotides","volume":"21 2","pages":"101-7"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/oli.2011.0278","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29716243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Oligo/polynucleotide-based gene modification: strategies and therapeutic potential. 基于寡核苷酸/多核苷酸的基因修饰:策略和治疗潜力。

Oligonucleotides Pub Date : 2011-03-01 Epub Date: 2011-03-21 DOI: 10.1089/oli.2010.0273

R Geoffrey Sargent, Soya Kim, Dieter C Gruenert

{"title":"Oligo/polynucleotide-based gene modification: strategies and therapeutic potential.","authors":"R Geoffrey Sargent, Soya Kim, Dieter C Gruenert","doi":"10.1089/oli.2010.0273","DOIUrl":"https://doi.org/10.1089/oli.2010.0273","url":null,"abstract":"Oligonucleotide- and polynucleotide-based gene modification strategies were developed as an alternative to transgene-based and classical gene targeting-based gene therapy approaches for treatment of genetic disorders. Unlike the transgene-based strategies, oligo/polynucleotide gene targeting approaches maintain gene integrity and the relationship between the protein coding and gene-specific regulatory sequences. Oligo/polynucleotide-based gene modification also has several advantages over classical vector-based homologous recombination approaches. These include essentially complete homology to the target sequence and the potential to rapidly engineer patient-specific oligo/polynucleotide gene modification reagents. Several oligo/polynucleotide-based approaches have been shown to successfully mediate sequence-specific modification of genomic DNA in mammalian cells. The strategies involve the use of polynucleotide small DNA fragments, triplex-forming oligonucleotides, and single-stranded oligodeoxynucleotides to mediate homologous exchange. The primary focus of this review will be on the mechanistic aspects of the small fragment homologous replacement, triplex-forming oligonucleotide-mediated, and single-stranded oligodeoxynucleotide-mediated gene modification strategies as it relates to their therapeutic potential.","PeriodicalId":19523,"journal":{"name":"Oligonucleotides","volume":"21 2","pages":"55-75"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/oli.2010.0273","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29752456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Antisense oligonucleotides targeting abhydrolase domain containing 2 block human hepatitis B virus propagation. 以含2的脱氢酶结构域为靶点的反义寡核苷酸可阻断乙型肝炎病毒的传播。

Oligonucleotides Pub Date : 2011-03-01 Epub Date: 2011-04-05 DOI: 10.1089/oli.2011.0280

Xiaoran Ding, Jing Yang, Shengqi Wang

{"title":"Antisense oligonucleotides targeting abhydrolase domain containing 2 block human hepatitis B virus propagation.","authors":"Xiaoran Ding, Jing Yang, Shengqi Wang","doi":"10.1089/oli.2011.0280","DOIUrl":"https://doi.org/10.1089/oli.2011.0280","url":null,"abstract":"Hepatitis B virus (HBV) infection is a major health concern worldwide and only a minority of treated patients develop a sustained protective response following a short course of therapy, and most patients require prolonged treatment to suppress viral replication. However, several recent reports showed that inhibition of certain host cell proteins prevented viral infection, specifically the human abhydrolase domain containing 2 (ABHD2) has been confirmed by our previous study to be upregulated in HepG2.2.15 cells but downregulated by lamivudine. These observations suggested that ABHD2 was important for HBV propagation and could be a target of novel anti-HBV drugs. To assess the importance of ABHD2 to the HBV infection process, antisense oligonucleotides (ASODNs) were used to downregulate ABHD2 expression in HepG2.2.15 cells. From 5 ASODNS candidates tested, AB3 significantly downregulated ABHD2 mRNA and protein expression levels. Further, AB3 significantly reduced HBV DNA, hepatitis B surface antigen, and hepatitis B \"e\" antigen protein expression levels in cell medium without affecting cell viability. These results suggest that downregulation of ABHD2 using ASODNs blocked HBV replication and expression without affecting host cell physiology. Further, data demonstrated an essential role of ABHD2 in HBV propagation, suggesting it can serve as a novel target for anti-HBV drug development.","PeriodicalId":19523,"journal":{"name":"Oligonucleotides","volume":"21 2","pages":"77-84"},"PeriodicalIF":0.0,"publicationDate":"2011-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/oli.2011.0280","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29792938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

A sol-gel-based microfluidics system enhances the efficiency of RNA aptamer selection. 基于溶胶-凝胶的微流体系统提高了RNA适体选择的效率。

Oligonucleotides Pub Date : 2011-03-01 Epub Date: 2011-03-17 DOI: 10.1089/oli.2010.0263

Ji-Young Ahn, Minjoung Jo, Pooja Dua, Dong-Ki Lee, Soyoun Kim

引用次数: 30

Development of single-stranded DNA aptamers for specific Bisphenol a detection. 用于双酚a特异性检测的单链DNA适体的研制。

Oligonucleotides Pub Date : 2011-03-01 Epub Date: 2011-03-17 DOI: 10.1089/oli.2010.0267

Minjoung Jo, Ji-Young Ahn, Joohyung Lee, Seram Lee, Sun Woo Hong, Jae-Wook Yoo, Jeehye Kang, Pooja Dua, Dong-Ki Lee, Seunghun Hong, Soyoun Kim

引用次数: 167

Cell-specific aptamer-mediated targeted drug delivery. 细胞特异性适配体介导的靶向药物递送。

Oligonucleotides Pub Date : 2011-02-01 Epub Date: 2010-12-23 DOI: 10.1089/oli.2010.0264

Jiehua Zhou, John J Rossi

引用次数: 154

T-oligos inhibit growth and induce apoptosis in human ovarian cancer cells. t -寡核苷酸抑制人卵巢癌细胞生长并诱导细胞凋亡。

Oligonucleotides Pub Date : 2011-02-01 Epub Date: 2011-01-31 DOI: 10.1089/oli.2010.0259

Sibaji Sarkar, Douglas V Faller

引用次数: 43