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Single Molecule Spectroscopy and Superresolution Imaging XII最新文献

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Front Matter: Volume 10884 封面:卷10884
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-05-22 DOI: 10.1117/12.2523428
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引用次数: 0
Super-resolution microscopy using deep learning (Conference Presentation) 使用深度学习的超分辨率显微镜(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-13 DOI: 10.1117/12.2509120
A. Ozcan
{"title":"Super-resolution microscopy using deep learning (Conference Presentation)","authors":"A. Ozcan","doi":"10.1117/12.2509120","DOIUrl":"https://doi.org/10.1117/12.2509120","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122111877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tunable structured illumination microscopy for acquisition of partially super-resolved images using a digital micro-mirror device (Conference Presentation) 使用数字微镜装置获取部分超分辨图像的可调谐结构照明显微镜(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-13 DOI: 10.1117/12.2507607
Taeseong Woo, Cheolwoo Ahn, Jung‐Hoon Park
{"title":"Tunable structured illumination microscopy for acquisition of partially super-resolved images using a digital micro-mirror device\u0000 (Conference Presentation)","authors":"Taeseong Woo, Cheolwoo Ahn, Jung‐Hoon Park","doi":"10.1117/12.2507607","DOIUrl":"https://doi.org/10.1117/12.2507607","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130751688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dissolution of single upconversion nanoparticles dispersed in aqueous buffers (Conference Presentation) 分散在水缓冲液中的单个上转化纳米颗粒的溶解(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-13 DOI: 10.1117/12.2511077
Y. Mély, Oleksii Dukhno, F. Przybilla, T. Hirsch
{"title":"Dissolution of single upconversion nanoparticles dispersed in aqueous buffers (Conference Presentation)","authors":"Y. Mély, Oleksii Dukhno, F. Przybilla, T. Hirsch","doi":"10.1117/12.2511077","DOIUrl":"https://doi.org/10.1117/12.2511077","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125874884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deep learning for dense and multicolor localization microscopy (Conference Presentation) 密集和多色定位显微镜的深度学习(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-13 DOI: 10.1117/12.2506499
E. Nehme, Eran Hershko, Lucien E. Weiss, T. Michaeli, Y. Shechtman
{"title":"Deep learning for dense and multicolor localization microscopy (Conference Presentation)","authors":"E. Nehme, Eran Hershko, Lucien E. Weiss, T. Michaeli, Y. Shechtman","doi":"10.1117/12.2506499","DOIUrl":"https://doi.org/10.1117/12.2506499","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124815991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Mapping molecules quantitatively in confocal fluorescence microscopy (Conference Presentation) 共聚焦荧光显微镜中的分子定量定位(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-04 DOI: 10.1117/12.2507391
M. Koenig, C. Berlage, Paja Reisch, F. Koberling, U. Ortmann, Haisen Ta, R. Erdmann
{"title":"Mapping molecules quantitatively in confocal fluorescence microscopy (Conference Presentation)","authors":"M. Koenig, C. Berlage, Paja Reisch, F. Koberling, U. Ortmann, Haisen Ta, R. Erdmann","doi":"10.1117/12.2507391","DOIUrl":"https://doi.org/10.1117/12.2507391","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125090380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single molecule imaging of fluorescence anisotropy (Conference Presentation) 荧光各向异性的单分子成像(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-04 DOI: 10.1117/12.2509832
Thomas Kavanagh, V. Devauges, Joseph Tideswell, Camille Perrin, Jin Li, J. Aluko, S. Poland, A. Beavil, S. Ameer-Beg
{"title":"Single molecule imaging of fluorescence anisotropy (Conference Presentation)","authors":"Thomas Kavanagh, V. Devauges, Joseph Tideswell, Camille Perrin, Jin Li, J. Aluko, S. Poland, A. Beavil, S. Ameer-Beg","doi":"10.1117/12.2509832","DOIUrl":"https://doi.org/10.1117/12.2509832","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132687882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increasing the sensitivity and resolution of single plane light sheet microscopy with multi-pulse pumping and time-gated detection (Conference Presentation) 利用多脉冲抽运和时间门控检测提高单平面光片显微镜的灵敏度和分辨率(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-04 DOI: 10.1117/12.2514298
J. Kimball, Zhangatay Nurekeyev, J. Chavez, L. Ceresa, S. Raut, I. Gryczynski, Z. Gryczynski
{"title":"Increasing the sensitivity and resolution of single plane light sheet microscopy with multi-pulse pumping and time-gated detection (Conference Presentation)","authors":"J. Kimball, Zhangatay Nurekeyev, J. Chavez, L. Ceresa, S. Raut, I. Gryczynski, Z. Gryczynski","doi":"10.1117/12.2514298","DOIUrl":"https://doi.org/10.1117/12.2514298","url":null,"abstract":"Optical microscopes have proven their use as a powerful tool for studying a variety of biological samples. In spite of many successes, there are still numerous obstacles limiting practical applications. Most limiting are the inherent background of physiological samples, photobleaching, and phototoxicity. To allow studies of long lasting processes such as drag delivery, three-dimensional cellular structures, embryogenesis, we have combined a technique called Single Plane Illumination Microscopy (SPIM) with Multi-Pulse Pumping with Time-Gated Detection (MPP-TGD) in order to enhance the signal relative to background. This new method allows for a decrease in light exposure times and improves image quality. This combination allows a new outlook into a variety of important, long-lasting biological processes at a level of detection previously unattainable. \u0000Multi-pulse pumping is a burst of excitation pulses instead of a single pulse which enhances the excited state population of a long-lived label. This label is chosen so that its lifetime is at least 5 times longer than that of typical autofluorescence. The pulse separation within the burst is chosen so that it is at least 5 times shorter than the lifetime of the label. In this case only the population of the fluorescent label is increased and the background remains the same. By subtracting the image acquired with the burst from an image with a single pulse, we were able to increase the signal-to-background ratio of about 100 fold.","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126336193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiphoton superresolution imaging using lateral-axial tomography and single-pixel spatial-frequency projections (Conference Presentation) 使用横向轴向断层成像和单像素空间频率投影的多光子超分辨率成像(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-04 DOI: 10.1117/12.2510204
K. Wernsing, J. Field, J. Squier, R. Bartels
{"title":"Multiphoton superresolution imaging using lateral-axial tomography and single-pixel spatial-frequency projections (Conference Presentation)","authors":"K. Wernsing, J. Field, J. Squier, R. Bartels","doi":"10.1117/12.2510204","DOIUrl":"https://doi.org/10.1117/12.2510204","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114300488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA intercalators tilt, wobble, and twirl: elucidating DNA overstretching (Conference Presentation) DNA插入器倾斜，摆动和旋转:阐明DNA过度拉伸(会议报告)
Single Molecule Spectroscopy and Superresolution Imaging XII Pub Date : 2019-03-04 DOI: 10.1117/12.2507066
Adam S. Backer, A. Biebricher, G. King, I. Heller, G. Wuite, E. Peterman
{"title":"DNA intercalators tilt, wobble, and twirl: elucidating DNA overstretching (Conference Presentation)","authors":"Adam S. Backer, A. Biebricher, G. King, I. Heller, G. Wuite, E. Peterman","doi":"10.1117/12.2507066","DOIUrl":"https://doi.org/10.1117/12.2507066","url":null,"abstract":"","PeriodicalId":194933,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XII","volume":"180 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133317041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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