Nucleosides, Nucleotides and Nucleic Acids最新文献

Nucleoside dimers analogs containing floxuridine and thymidine with unnatural linker groups: synthesis and cancer line studies. Part III 含有氟尿定和胸腺嘧啶的核苷二聚体类似物与非自然连接基团:合成和癌症系研究。第三部分

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2019-08-05 DOI: 10.1080/15257770.2019.1641206

D. Baraniak, P. Ruszkowski, Daniel Baranowski, G. Framski, J. Boryski

引用次数: 5

Substrate specificity of E. coli uridine phosphorylase. Further evidences of high-syn conformation of the substrate in uridine phosphorolysis 大肠杆菌尿苷磷酸化酶的底物特异性。尿苷磷解中底物高同步构象的进一步证据

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-02-01 DOI: 10.1080/15257770.2016.1223306

C. S. Alexeev, G. Sivets, T. Safonova, S. Mikhailov

{"title":"Substrate specificity of E. coli uridine phosphorylase. Further evidences of high-syn conformation of the substrate in uridine phosphorolysis","authors":"C. S. Alexeev, G. Sivets, T. Safonova, S. Mikhailov","doi":"10.1080/15257770.2016.1223306","DOIUrl":"https://doi.org/10.1080/15257770.2016.1223306","url":null,"abstract":"ABSTRACT Twenty five uridine analogues have been tested and compared with uridine with respect to their potency to bind to E. coli uridine phosphorylase. The kinetic constants of the phosphorolysis reaction of uridine derivatives modified at 2′-, 3′- and 5′-positions of the sugar moiety and 2-, 4-, 5- and 6-positions of the heterocyclic base were determined. The absence of the 2′- or 5′-hydroxyl group is not crucial for the successful binding and phosphorolysis. On the other hand, the absence of both the 2′- and 5′-hydroxyl groups leads to the loss of substrate binding to the enzyme. The same effect was observed when the 3′-hydroxyl group is absent, thus underlining the key role of this group. Our data shed some light on the mechanism of ribo- and 2′-deoxyribonucleoside discrimination by E. coli uridine phosphorylase and E. coli thymidine phosphorylase. A comparison of the kinetic results obtained in the present study with the available X-ray structures and analysis of hydrogen bonding in the enzyme-substrate complex demonstrates that uridine adopts an unusual high-syn conformation in the active site of uridine phosphorylase.","PeriodicalId":19306,"journal":{"name":"Nucleosides, Nucleotides and Nucleic Acids","volume":"13 1","pages":"107 - 121"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79557198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Intermolecular interaction of nickel (ii) phthalocyanine tetrasulfonic acid tetrasodium salt with bovine serum albumin: A multi-technique study 镍(ii)酞菁四磺酸四钠盐与牛血清白蛋白的分子间相互作用:一项多技术研究

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-02-01 DOI: 10.1080/15257770.2016.1226338

H. Dezhampanah, R. Firouzi, L. Hasani

{"title":"Intermolecular interaction of nickel (ii) phthalocyanine tetrasulfonic acid tetrasodium salt with bovine serum albumin: A multi-technique study","authors":"H. Dezhampanah, R. Firouzi, L. Hasani","doi":"10.1080/15257770.2016.1226338","DOIUrl":"https://doi.org/10.1080/15257770.2016.1226338","url":null,"abstract":"ABSTRACT The interaction of nickel (II) phthalocyanine tetrasulfonic acid tetrasodium salt with bovine serum albumin (BSA) has been investigated by combination of fluorescence, UV-vis absorption, Fourier transform infrared (FT-IR), and circular dichorism (CD) spectroscopies as well as through molecular docking. Fluorescence quenching and absorption spectra were investigated as a mean for estimating the binding parameters. Analysis of fluorescence quenching data at different temperatures was performed in order to specify the thermodynamics parameters for interactions of phthalocyanine complex with BSA. According to experimental data it was suggested that phthalocyanine had a significant binding affinity to BSA and the process was entropy driven. Based on the results of molecular docking it was indicated that the main active binding site for this phthalocyanine complex is site I in subdomain IIA of BSA. The results provide useful information for understanding the binding mechanism of anticancer drug-albumin and gives insight into the biological activity and metabolism of the drug in blood.","PeriodicalId":19306,"journal":{"name":"Nucleosides, Nucleotides and Nucleic Acids","volume":"24 1","pages":"122 - 138"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86488992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Multispectroscopic studies on the interaction of a copper(ii) complex of ibuprofen drug with calf thymus DNA 布洛芬铜(ii)配合物与小牛胸腺DNA相互作用的多光谱研究

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-02-01 DOI: 10.1080/15257770.2016.1223305

N. Shahabadi, Farshad Shiri

{"title":"Multispectroscopic studies on the interaction of a copper(ii) complex of ibuprofen drug with calf thymus DNA","authors":"N. Shahabadi, Farshad Shiri","doi":"10.1080/15257770.2016.1223305","DOIUrl":"https://doi.org/10.1080/15257770.2016.1223305","url":null,"abstract":"ABSTRACT The interaction of copper(II)–ibuprofenato complex with calf thymus DNA (ct-DNA) has been explored following, UV-visible spectrophotometry, fluorescence measurement, dynamic viscosity measurements, and circular dichroism spectroscopy. In spectrophotometric studies of ct-DNA it was found that [Cu(ibp)2]2 can form a complex with double-helical DNA. The association constant of [Cu(ibp)2]2 with DNA from UV-Vis study was found to be 6.19 × 104 L mol−1. The values of Kf from fluorescence measurement clearly underscore the high affinity of [Cu(ibp)2]2 to DNA. The experimental results showed that the conformational changes in DNA helix induced by [Cu(ibp)2]2 are the reason for the fluorescence quenching of the DNA-Hoechst system. In addition, the fluorescence emission spectra of intercalated methylene blue (MB) with increasing concentrations of [Cu(ibp)2]2 represented a significant increase of MB intensity as to release MB from MB-DNA system. The results of circular dichroism (CD) suggested that copper(II)–ibuprofenato complex can change the conformation of DNA. In addition, the results of viscosity measurements suggest that copper(II)–ibuprofenato complex may bind with non-classical intercalative mode. From spectroscopic and hydrodynamic studies, it has been found that [Cu(ibp)2]2 interacts with DNA by partial intercalation mode which contains intercalation and groove properties. GRAPHICAL ABSTRACT","PeriodicalId":19306,"journal":{"name":"Nucleosides, Nucleotides and Nucleic Acids","volume":"2 1","pages":"106 - 83"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76217516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

A neurodevelopmental disorder with a nonsense mutation in the Ox-2 antigen domain of the amyloid precursor protein (APP) gene 淀粉样蛋白前体蛋白(APP)基因Ox-2抗原域无义突变的一种神经发育障碍

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-01-19 DOI: 10.1080/15257770.2016.1267361

K. Nguyen, K. Leydiker, Raymond Y. Wang, J. Abdenur, W. Nyhan

{"title":"A neurodevelopmental disorder with a nonsense mutation in the Ox-2 antigen domain of the amyloid precursor protein (APP) gene","authors":"K. Nguyen, K. Leydiker, Raymond Y. Wang, J. Abdenur, W. Nyhan","doi":"10.1080/15257770.2016.1267361","DOIUrl":"https://doi.org/10.1080/15257770.2016.1267361","url":null,"abstract":"ABSTRACT We report a patient, an infant with a neurodevelopmental disorder manifesting intractable complex partial epilepsy, bull's eye maculopathy, microcephaly, bilateral cataracts, truncal hypotonia, and spasticity of all four extremities. Sequencing of genomic DNA revealed mutations in (a) exon 8 (Ox-2 antigen domain) of the amyloid precursor protein (APP) gene: c.1075C>T, p.Arg359* (b) exon 8 of the senataxin (SETX) gene: c.4738C>T, p.Arg1580Cys, and (c) exon 2 of the ceroid-lipofuscinosis, neuronal 8 (CLN8) gene: c.685C>G, p.Pro229Ala. Using a quantitative method for measurement of various APP-mRNA isoforms, we found that the APP-mRNA isoform of 624 bp with a deletion starting after 49 bp of the 5′ end of exon 3 followed by a complete deletion of exons 4–15, mutations in exon 1: c.22C>T, p.L18F, and exon 3: c.269A>G, p.Q90R encoding APP207 isoform was the most abundant one, and would appear to be responsible for the clinical manifestations. This is the first example that may underline the role of the epigenetic regulation in the expression of APP gene leading to a neurodevelopmental disorder resulting from a nonsense mutation in the Ox-2 antigen domain.","PeriodicalId":19306,"journal":{"name":"Nucleosides, Nucleotides and Nucleic Acids","volume":"112 1","pages":"317 - 327"},"PeriodicalIF":0.0,"publicationDate":"2017-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80766285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Pyrimidine non-nucleoside analogs: A direct synthesis of a novel class of N-substituted amino and N-sulfonamide derivatives of pyrimidines 嘧啶非核苷类似物:直接合成一类新的n-取代氨基和n-磺胺嘧啶衍生物

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-01-19 DOI: 10.1080/15257770.2016.1257808

G. Elgemeie, A. Salah, N. S. Abbas, H. Hussein, Reham A. Mohamed

引用次数: 11

Synthesis, stereochemical characterization, and antimicrobial evaluation of a potentially nonnephrotoxic 3′-C-acethydrazide puromycin analog 一种可能无肾毒性的3 ' - c -乙酰肼嘌呤霉素类似物的合成、立体化学表征和抗菌评价

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-01-19 DOI: 10.1080/15257770.2016.1264590

J. Carter, Blair Weaver, M. Chiacchio, Amy R. Messersmith, W. Lynch, Brent D. Feske, G. Gumina

引用次数: 1

Synthesis and antibacterial activity of 5′-tetrachlorophthalimido and 5′-azido 5′-deoxyribonucleosides 5′-四氯邻苯二胺和5′-叠氮5′-脱氧核糖核苷的合成及抗菌活性研究

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-01-03 DOI: 10.1080/15257770.2016.1250906

Robert P Van Ostrand, Casey Jacobsen, A. Delahunty, Carley Stringer, Ryan R. Noorbehesht, Haidi Ahmed, A. Awad

引用次数: 3

New N-substituted hydrazones, derivatives of uridyl aldehyde 新的n取代腙，尿苷醛衍生物

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-01-03 DOI: 10.1080/15257770.2016.1231321

Katarzyna Kral, T. Bieg, A. Kudelko, A. Barabaś, A. Dąbrowska, I. Wandzik

引用次数: 0

Nucleic acid components and their analogs: Design and synthesis of novel cytosine thioglycoside analogs 核酸组分及其类似物:新型胞嘧啶巯基糖苷类似物的设计与合成

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2017-01-03 DOI: 10.1080/15257770.2016.1231318

G. Elgemeie, A. Salah, N. S. Abbas, H. Hussein, Reham A. Mohamed

引用次数: 13