Neurosignals最新文献

{"title":"Copper-zinc superoxide dismutase (SOD1) is released by microglial cells and confers neuroprotection against 6-OHDA neurotoxicity.","authors":"Elisabetta Polazzi,&nbsp;Ilaria Mengoni,&nbsp;Marco Caprini,&nbsp;Emiliano Peña-Altamira,&nbsp;Ewelina Kurtys,&nbsp;Barbara Monti","doi":"10.1159/000337115","DOIUrl":"https://doi.org/10.1159/000337115","url":null,"abstract":"<p><p>Microglial-neuronal interactions are essential for brain physiopathology. In this framework, recent data have changed the concept of microglia from essentially macrophagic cells to crucial elements in maintaining neuronal homeostasis and function through the release of neuroprotective molecules. Using proteomic analysis, here we identify copper-zinc superoxide dismutase (SOD1) as a protein produced and released by cultured rat primary microglia. Evidence for a neuroprotective role of microglia-derived SOD1 resulted from experiments in which primary cerebellar granule neurons (CGNs) were exposed to the dopaminergic toxin 6-hydroxydopamine (6-OHDA). Microglial conditioned medium, in which SOD1 had accumulated, protected CGNs from degeneration, and neuroprotection was abrogated by SOD1 inhibitors. These effects were replicated when exogenous SOD1 was added to a nonconditioned medium. SOD1 neuroprotective action was mediated by increased cell calcium from an external source. Further experiments demonstrated the specificity of SOD1 neuroprotection against 6-OHDA compared to other types of neurotoxic challenges. SOD1, constitutively produced and released by microglia through a lysosomal secretory pathway, is identified here for the first time as an essential component of neuroprotection mediated by microglia. This novel information is relevant to stimulating further studies of microglia-mediated neuroprotection in in vivo models of neurodegenerative diseases.</p>","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"21 1-2","pages":"112-28"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000337115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30606486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional recovery of human cells harbouring the mitochondrial DNA mutation MERRF A8344G via peptide-mediated mitochondrial delivery.","authors":"Jui-Chih Chang,&nbsp;Ko-Hung Liu,&nbsp;Yu-Chi Li,&nbsp;Shou-Jen Kou,&nbsp;Yau-Huei Wei,&nbsp;Chieh-Sen Chuang,&nbsp;Mingli Hsieh,&nbsp;Chin-San Liu","doi":"10.1159/000341981","DOIUrl":"https://doi.org/10.1159/000341981","url":null,"abstract":"<p><p>We explored the feasibility of mitochondrial therapy using the cell-penetrating peptide Pep-1 to transfer mitochondrial DNA (mtDNA) between cells and rescue a cybrid cell model of the mitochondrial disease myoclonic epilepsy with ragged-red fibres (MERRF) syndrome. Pep-1-conjugated wild-type mitochondria isolated from parent cybrid cells incorporating a mitochondria-specific tag were used as donors for mitochondrial delivery into MERRF cybrid cells (MitoB2) and mtDNA-depleted Rho-zero cells (Mitoρ°). Forty-eight hours later, translocation of Pep-1-labelled mitochondria into the mitochondrial regions of MitoB2 and Mitoρ° host cells was observed (delivery efficiencies of 77.48 and 82.96%, respectively). These internalized mitochondria were maintained for at least 15 days in both cell types and were accompanied by mitochondrial function recovery and cell survival by preventing mitochondria-dependent cell death. Mitochondrial homeostasis analyses showed that peptide-mediated mitochondrial delivery (PMD) also increased mitochondrial biogenesis in both cell types, but through distinct regulatory pathways involving mitochondrial dynamics. Dramatic decreases in mitofusin-2 (MFN2) and dynamin-related protein 1/fission 1 were observed in MitoB2 cells, while Mitoρ° cells showed a significant increase in optic atrophy 1 and MFN2. These findings suggest that PMD can be used as a potential therapeutic intervention for mitochondrial disorders.</p>","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"21 3-4","pages":"160-73"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000341981","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30929874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of levodopa and deep brain stimulation on subthalamic local field low-frequency oscillations in Parkinson's disease.","authors":"Gaia Giannicola,&nbsp;Manuela Rosa,&nbsp;Sara Marceglia,&nbsp;Emma Scelzo,&nbsp;Lorenzo Rossi,&nbsp;Domenico Servello,&nbsp;Claudia Menghetti,&nbsp;Claudio Pacchetti,&nbsp;Roberta Zangaglia,&nbsp;Marco Locatelli,&nbsp;Elena Caputo,&nbsp;Filippo Cogiamanian,&nbsp;Gianluca Ardolino,&nbsp;Sergio Barbieri,&nbsp;Alberto Priori","doi":"10.1159/000336543","DOIUrl":"https://doi.org/10.1159/000336543","url":null,"abstract":"<p><p>New adaptive systems for deep brain stimulation (DBS) could in the near future optimize stimulation settings online so as to achieve better control over the clinical fluctuations in Parkinson's disease (PD). Local field potentials (LFPs) recorded from the subthalamic nucleus (STN) in PD patients show that levodopa and DBS modulate STN oscillations. Because previous research has shown that levodopa and DBS variably influence beta LFP activity (8-20 Hz), we designed this study to find out how they affect low-frequency (LF) oscillations (2-7 Hz). STN LFPs were recorded in 19 patients with PD during DBS, after levodopa medication, and during DBS and levodopa intake combined. We investigated the relationship between LF modulations, DBS duration and levodopa intake. We also studied whether LF power depended on disease severity, the patient's clinical condition and whether LF modulations were related to electrode impedances. LF power increased during DBS, after levodopa intake and under both experimental conditions combined. The LF power increase correlated with the levodopa-induced clinical improvement and the higher the electrode impedance, the greater was the LF power change. These data suggest that the LF band could be useful as a control neurosignal for developing novel adaptive DBS systems for patients with PD.</p>","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":" ","pages":"89-98"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000336543","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40185594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"K. Schenck-Gustafsson, P. DeCola, D. Pfaff, D. Pisetsky","doi":"10.1159/000345868","DOIUrl":"https://doi.org/10.1159/000345868","url":null,"abstract":"The abstract should concisely present the hypothesis being tested, general methods, results and conclusions. Each abstract should contain no more than 200 words. Introduction: This section must contain a clear statement of the aims of the work or of the hypotheses being tested. A brief account of the relevant background, which supports the rationale of the study, should also be given. The length of the introduction should not exceed 750 words. Materials and Methods: This section should contain explicit, concise descriptions of all methods or procedures employed. Descriptions of methods must be sufficient to enable the reader to judge the accuracy, reproducibility and reliability of the experiment(s). Results: Contained in this section are the experimental data, with no discussion of their significance. Sufficient data should be presented to allow for judgement of the variability and reliability of the results. Discussion: Conclusions drawn from the results presented are included in this section. The Discussion must be as concise as possible and should not exceed 1,500 words. Footnotes: Avoid footnotes. When essential, they are to be numbered consecutively and typed at the foot of the appropriate page. Acknowledgements: Including, where relevant, credit to the sources of grant support, should be placed before the references. Tables and illustrations: Tables and illustrations (both numbered in Arabic numerals) should be prepared on separate sheets. Tables require a heading and figures a legend, also prepared on a separate sheet. For the reproduction of illustrations, only good drawings and original photographs can be accepted; negatives or photocopies cannot be used. Due to technical reasons, figures with a screen background should not be submitted. When possible, group several illustrations on one block for reproduction (max. size 180  223 mm) or provide crop marks. On the back of each illustration, indicate its number, the author’s name, and ‘top’ with a soft pencil. Electronically submitted b/w half-tone and color illustrations must have a final resolution of 300 dpi after scaling, line drawings one of 800–1,200 dpi. Color illustrations Online edition: Color illustrations are reproduced free of charge online; however, in the print version, the illustrations are reproduced in black and white, unless color is paid for. Please avoid referring to the colors in the text and figure legends. Print edition: Up to 6 color illustrations per page can be integrated within the text at CHF 800.– per page. References: In the text, identify references by Arabic numerals [in square brackets]. Material submitted for publication but not yet accepted should be noted as ‘unpublished data’ and not be included in the reference list. The list of references should include only those publications which are cited in the text. Do not alphabetize; number references in the order in which they are first mentioned in the text. The surnames of the authors followed by initials ","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000345868","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64630287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contents Vol. 20, 2012","authors":"N. Hyun, Kwang-Ho Hyun, Kyungmin Lee, B. Kaang, Hyun-Jung Koo, Y. Piao, Y. Pak, K. Mak, A. C. Lo, A. Lam, P. Yeung, B. C. Ko, S. Chung, Sookja K. Chung, Xu-ping Li, W. Xie, Zhen Zhang, Sagar Kansara, J. Jankovic, W. Le, Satz Mengensatzproduktion, D. R. Basel","doi":"10.1159/000345230","DOIUrl":"https://doi.org/10.1159/000345230","url":null,"abstract":"","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"20 1","pages":"I - IV"},"PeriodicalIF":0.0,"publicationDate":"2012-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64623574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Author Index Vol. 20, No. 3, 2012","authors":"","doi":"10.1159/000339232","DOIUrl":"https://doi.org/10.1159/000339232","url":null,"abstract":"","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"20 1","pages":"221 - 221"},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000339232","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64594019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Title Page / Table of Contents","authors":"","doi":"10.1159/000339229","DOIUrl":"https://doi.org/10.1159/000339229","url":null,"abstract":"","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"20 1","pages":"127 - 129"},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000339229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64593907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"","doi":"10.1159/000339719","DOIUrl":"https://doi.org/10.1159/000339719","url":null,"abstract":"","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000339719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64596514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subject Index Vol. 20, No. 3, 2012","authors":"","doi":"10.1159/000339233","DOIUrl":"https://doi.org/10.1159/000339233","url":null,"abstract":"","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"20 1","pages":"222 - 222"},"PeriodicalIF":0.0,"publicationDate":"2012-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000339233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64594101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"","doi":"10.1159/000338006","DOIUrl":"https://doi.org/10.1159/000338006","url":null,"abstract":"The abstract should concisely present the hypothesis being tested, general methods, results and conclusions. Each abstract should contain no more than 200 words. Introduction: This section must contain a clear statement of the aims of the work or of the hypotheses being tested. A brief account of the relevant background, which supports the rationale of the study, should also be given. The length of the introduction should not exceed 750 words. Materials and Methods: This section should contain explicit, concise descriptions of all methods or procedures employed. Descriptions of methods must be sufficient to enable the reader to judge the accuracy, reproducibility and reliability of the experiment(s). Results: Contained in this section are the experimental data, with no discussion of their significance. Sufficient data should be presented to allow for judgement of the variability and reliability of the results. Discussion: Conclusions drawn from the results presented are included in this section. The Discussion must be as concise as possible and should not exceed 1,500 words. Footnotes: Avoid footnotes. When essential, they are to be numbered consecutively and typed at the foot of the appropriate page. Acknowledgements: Including, where relevant, credit to the sources of grant support, should be placed before the references. Tables and illustrations: Tables and illustrations (both numbered in Arabic numerals) should be prepared on separate sheets. Tables require a heading and figures a legend, also prepared on a separate sheet. For the reproduction of illustrations, only good drawings and original photographs can be accepted; negatives or photocopies cannot be used. Due to technical reasons, figures with a screen background should not be submitted. When possible, group several illustrations on one block for reproduction (max. size 180  223 mm) or provide crop marks. On the back of each illustration, indicate its number, the author’s name, and ‘top’ with a soft pencil. Electronically submitted b/w half-tone and color illustrations must have a final resolution of 300 dpi after scaling, line drawings one of 800–1,200 dpi. Color illustrations Online edition: Color illustrations are reproduced free of charge online; however, in the print version, the illustrations are reproduced in black and white, unless color is paid for. Please avoid referring to the colors in the text and figure legends. Print edition: Up to 6 color illustrations per page can be integrated within the text at CHF 800.– per page. References: In the text, identify references by Arabic numerals [in square brackets]. Material submitted for publication but not yet accepted should be noted as ‘unpublished data’ and not be included in the reference list. The list of references should include only those publications which are cited in the text. Do not alphabetize; number references in the order in which they are first mentioned in the text. The surnames of the authors followed by initials ","PeriodicalId":19171,"journal":{"name":"Neurosignals","volume":"111 3S 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000338006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64586476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}