Molecular Genetics, Microbiology and Virology最新文献

{"title":"The Genetic Predisposition of Human and Laboratory Animals to Different Infections Transmitted by Ixodid Ticks","authors":"A. V. Barkhash","doi":"10.3103/s0891416822020021","DOIUrl":"https://doi.org/10.3103/s0891416822020021","url":null,"abstract":"<p>Ixodid ticks are carriers of causative agents of infectious diseases dangerous to humans, including tick-borne encephalitis, ixodid tick-borne borrelioses, rickettsioses, human monocytic ehrlichiosis, human granulocytic anaplasmosis, Congo–Crimean hemorrhagic fever, Omsk hemorrhagic fever, Q fever, and tularemia. These natural focal diseases represent a significant medical problem. It is known that an organism’s response to the effect of an infectious agent (and, as a consequence, peculiarities of the course and outcome of the disease) depends not only on the characteristics of the causative agent and external factors, but also significantly on the individual characteristics of the host (particularly, human) genome that predetermine the ability of its immune system to suppress the development of infection. There are two main approaches to the search for human genes predetermining the degree of his susceptibility/resistance to infectious agents. First, the analysis of candidate genes that are presumably relevant to the development of this disease based on known or putative functions of their protein products or on previous data on the orthologous genes in model organisms. Second, genome-wide association analysis, in which a large number of genetic markers evenly distributed across the human genome are simultaneously analyzed. The human genetic predisposition to such infectious diseases as AIDS, tuberculosis, malaria, hepatitis C and B, etc., has been intensively studied, and human genetic factors that specifically predetermine the efficiency of protective reactions against these infections have been already identified. As for infections transmitted by ixodid ticks, the genes of human predisposition to them are those far less studied, and the existing data are rather scattered. This review summarizes already-known genetic factors associated with predisposition to different infections transmitted by ixodid ticks in beings human and laboratory animals, as well as approaches to identification of these factors.</p>","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single Nucleotide Polymorphisms in the U86 Gene of Human betaherpesvirus 6B","authors":"A. Vyazovaya, O. Narvskaya, V. Vedernikov, M. A. Nikolskiy","doi":"10.3103/s0891416822030090","DOIUrl":"https://doi.org/10.3103/s0891416822030090","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87480096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epstein–Barr Virus: Evaluation of gp350 and EBNA2 Gene Variability","authors":"T. V. Solomay, M. Malakhova, E. Shitikov, D. Bespiatykh, V. Veselovsky, T. A. Semenenko, D. Smirnova, A. Gracheva, E. B. Fayzuloev","doi":"10.17116/molgen20224003132","DOIUrl":"https://doi.org/10.17116/molgen20224003132","url":null,"abstract":"Abstract Approximately 90% of the world’s population is infected with the Epstein–Barr virus (EBV). Damage to health and high economical costs require the development of vaccines against EBV. The variability of individual genes may affect the success of the vaccination campaign and will necessitate dynamic update of antigenic composition of vaccines. The aim of this study was to assess the variability of EBV gp350 and EBNA2 genes isolated from saliva of dental clinic personnel in Moscow oblast. Biosamples were obtained from 105 employees at four dental clinics. For EBV DNA-positive samples, the EBNA2 and gp350 genes were amplified using nested PCR. The nucleotide sequence of gp350 gene was determined during sequencing. A maximum likelihood phylogenetic tree was constructed from the N-terminal fragment of gp350 protein, using B95-8 strain as a reference genome. Phylogenetic analysis also included 222 EBV samples from the NCBI database. The EBNA2 genotype and the whole gp350 gene sequence were determined for 31 DNA samples. Based on the phylogenetic analysis of gp350 protein, the Russian virus population was uniformly distributed along the tree. Meanwhile, 30 samples fell into the Aa clade (A, genotype A for EBNA2 gene; a, similarity of the gp350 sequence with B95-8 [NC_007605.1]) and one sample belonged to the Bb clade (B, genotype B for EBNA2 ; b , similarity of gp350 with Jijoye [LN827800.1]). For 30 Russian samples of Aa genotype, 22 individual profiles and 16 unique mutations were found. Identical gp350 profiles were found amongst staff working in close professional contact. The identified features indicated the need for further phylogenetic studies of samples collected in Russia to develop and introduce vaccines and monitor their efficiency.","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79381847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Markers of Viral Hepatitis B in Blood–Plasma Samples of the Indigenous Population of the Far North of Russia. HBV Genotypes and HBsAg Subtypes","authors":"L. V. Bezuglova, L. Osipova, E. Sergeeva, I. Deliy, L. Tabikhanova, S. Netesov, I. Netesova","doi":"10.17116/molgen20224003141","DOIUrl":"https://doi.org/10.17116/molgen20224003141","url":null,"abstract":"Hepatitis B is a major public health problem worldwide. The indigenous population of remote regions with harsh living conditions, such as the Far North of Russia, with possibly unique genetic mutations in the local strains of this pathogen, remains poorly studied. The aim of the study was to determine the prevalence of serological HBV markers in blood–plasma samples of the population of the Far North of Russia and to determine HBV genotypes and HBsAg subtypes in blood–plasma samples containing HBsAg. We studied 702 blood–plasma samples taken from the indigenous population (Tundra Nenets) of the village of Gyda in the Tazovsky district of the Yamalo-Nenets autonomous okrug (YaNAO) (north of the Gydan Peninsula). Detection of serological HBV markers—HBsAg, anti-HBs, and anti-Hbcore—was carried out using JSC Vector-Best reagent kits. HBV genotypes and HBsAg subtypes in the samples were determined according to our own original procedure with monoclonal antibodies and by classical molecular-biological methods. The prevalence of HBsAg in the entire sample was 0.4% (3/702). For the subgroup of samples (persons older than 15 years during the first two expeditions, 300 samples), an extended analysis of the markers was carried out. The following frequencies were determined for them: HBsAg, 0.7% (2/300); anti-HBs, 63.7% (191/300); and anti-HBcore, 11.3% (34/300). HBV statuses were established for this subgroup: the presence of HBV infection (0.7%), past hepatitis B infection (9.3%), and immunity as result of vaccination (54.3%). Genotype D was established for two samples containing HBsAg (subtypes ayw2 and ayw3); the results of the two used methods coincided. The prevalence of HBsAg in the Tazovsky and adjacent Purovsky districts (the main population being Nenets, previously published data) differed (0.4% vs. 1.7%, p < 0.05), presumably due to vaccination (samples of the Purovsky district were collected almost 20 years earlier), as well as the distribution of HBV genotypes. The Tazovsky district of YaNAO is now a low endemic region in terms of HBV infection. The HBV isolates belong to the most common HBV genotype D in the Russian Federation (HBsAg subtypes ayw2, ayw3).","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76202426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of the Apoptotic Activity of CpsA-CpsC-L-ACAN Fusion Peptide against HeLa Cell Line","authors":"Elmira Babakanrad, T. Mohammadian, D. Esmaeili, P. Behzadi","doi":"10.3103/S089141682203003X","DOIUrl":"https://doi.org/10.3103/S089141682203003X","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72861533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive Immunity Systems of Bacteria: Association with Self-Synthesizing Transposons, Polyfunctionality","authors":"T. Ilyina","doi":"10.3103/s0891416822030065","DOIUrl":"https://doi.org/10.3103/s0891416822030065","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80513597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular-Genetic Monitoring of the Mycobacterium tuberculosis Population in Murmansk Oblast","authors":"A. Vyazovaya, N. Gavrilova, A. Gerasimova, A. Bychkova, I. Avadenii, E. Anikieva, N. Solovieva, V. Zhuravlev, I. Mokrousov, O. Narvskaya","doi":"10.3103/S0891416822020070","DOIUrl":"https://doi.org/10.3103/S0891416822020070","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76220342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Investigation of Clone and Expression of Butyrylcholinesterase in Pichia pastoris for Parathion Hydrolysis","authors":"M. Rastegar Shariat Panahi, M. Mirzaei, H. Tebyaniyan, S. Khodi, M. Heiat, A. Latifi","doi":"10.3103/S0891416822020057","DOIUrl":"https://doi.org/10.3103/S0891416822020057","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87462552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability of Multiple Resistance to Antibiotics in a Cholera Agent Associated with Different Types of SXT Element and Spontaneous Chromosome Mutations","authors":"N. Smirnova, D. A. Rybal’chenko, E. Shchelkanova, Yu. V. Lozovsky, Ya. M. Krasnov, V. Kutyrev","doi":"10.3103/S0891416822020069","DOIUrl":"https://doi.org/10.3103/S0891416822020069","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79435428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Aspects of Mechanosensitivity in the Alphaproteobacteria Azospirillum baldaniorum with Mixed Flagellation","authors":"S. Evstigneeva, D. Mokeev, L. Petrova, A. Shelud’ko","doi":"10.3103/S0891416822020045","DOIUrl":"https://doi.org/10.3103/S0891416822020045","url":null,"abstract":"","PeriodicalId":19005,"journal":{"name":"Molecular Genetics, Microbiology and Virology","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83817715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}