Junjuan Wang , Mengzhen Hao , QianWei Wang , Manman Liu , Guirong Liu , Shiwen Han , Xiaoyan Zhao , Huilian Che
{"title":"The conformational epitope of Ara h 5 was crucial to the severe reactivity of peanut allergy","authors":"Junjuan Wang , Mengzhen Hao , QianWei Wang , Manman Liu , Guirong Liu , Shiwen Han , Xiaoyan Zhao , Huilian Che","doi":"10.1016/j.molimm.2024.10.004","DOIUrl":"10.1016/j.molimm.2024.10.004","url":null,"abstract":"<div><div>Conformational epitopes are associated with the development of food allergic tolerance and the severity of food allergy. Peanut can trigger severe anaphylactic reactions, however, the reason behind the severe allergic reactions caused by peanut remains unexplained. The purpose of this article was to provide an explanation for the severe allergy caused by peanut, focusing on the conformational epitopes of Ara h 5 and Ara h 8 allergens that exhibit cross-reactivity with asthma reactions. Ara h 5 and Ara h 8 proteins were prepared by an Escherichia coli expression system. IgE reactivity of 37 patients with allergy toward Ara h 5 and Ara h 8 allergens was assessed by using IgE-binding assays, dot blot and western blot. The allergenicity of Ara h 5 and Ara h 8 protein was analysed in mouse model. Conformational IgE epitopes of Ara h 5 was identified using phage peptide library and the Pepitope Server. Compared to Ara h 8, the conformational epitope of Ara h 5 protein was crucial in the process of sensitization. Ara h 5 showed a stronger IgE reactivity and the ability to induce β-hexosaminidase release. Ara h 5 caused more severe lung inflammation than Ara h 8 protein. While Ara h 8 caused more severe intestinal inflammation than Ara h 5. The results showed that the conformational epitope sequences of Ara h 5 were WETIYSR and FHWWYLK. The results provide a theoretical basis for the production of hypoallergenic peanut protein and the immunotherapy of peanut allergy.</div></div>","PeriodicalId":18938,"journal":{"name":"Molecular immunology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Huizhong Li , Fei Wang , Haifang Zhao , Jiale Cao , Shiyuan Wang , Hongxia Li , Barbara Savoldo , Enyu Rao , Gianpietro Dotti , Hongwei Du
{"title":"Preclinical assessment of the efficacy of B7-H3 CAR-T in renal cell carcinoma","authors":"Huizhong Li , Fei Wang , Haifang Zhao , Jiale Cao , Shiyuan Wang , Hongxia Li , Barbara Savoldo , Enyu Rao , Gianpietro Dotti , Hongwei Du","doi":"10.1016/j.molimm.2024.10.006","DOIUrl":"10.1016/j.molimm.2024.10.006","url":null,"abstract":"<div><div>B7-H3 is a type I transmembrane protein that belongs to the B7 immune checkpoint protein family, is aberrantly expressed in cancer cells, but rarely expressed in normal tissues, making it an attractive target for cancer therapy. Here, we found B7-H3 is highly expressed in the renal cell carcinoma (RCC) tumor tissues and RCC cell lines, but is undetectable in normal renal tissues. Therefore, we engineered second-generation CAR-T cells targeting B7-H3, incorporating either CD28 or 4–1BB co-stimulatory domains. Both CAR-T cell variants demonstrated potent antitumor activity against RCC tumors in vitro and in metastatic and orthotopic RCC mouse models. Furthermore, the B7-H3 CAR-T cells exhibited remarkable proliferation and robust cytokine release when co-cultured with RCC cancer cells. These findings demonstrated that targeting B7-H3 by CAR-T cells potentially offering a new treatment option for RCC patients.</div></div>","PeriodicalId":18938,"journal":{"name":"Molecular immunology","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}