Mutagenesis最新文献

{"title":"DNA damage in the leukocytes of nanocomposite-exposed workers.","authors":"Pavel Rossner, Bozena Novotna, Daniela Pelclova, Vladimir Zdimal, Lucie Lischkova, Pavlina Klusackova, Jakub Ondracek, Jaroslav Schwarz, Ludmila Maskova, Stepanka Dvorackova, Alena Milcova, Antonin Ambroz, Andrea Rossnerova","doi":"10.1093/mutage/geag021","DOIUrl":"10.1093/mutage/geag021","url":null,"abstract":"<p><p>Human exposure to nanoparticles (NPs) poses potential health risks that are modulated, for example, by the physico-chemical characteristics of NPs, the exposure route and the dose of NPs. Among 21 workers involved for many years in welding on metal surfaces and the machining of nanocomposites, we investigated the role of NPs in the induction of DNA damage. Blood samples of the exposed subjects were collected before and after their working shifts. The controls (21 individuals without occupational exposer to NPs) were sampled in parallel with the exposed ones. NP exposure concentrations were assessed using stationary and personal monitors. DNA damage, including oxidized bases and strand breaks (DNA-SB), in leukocytes was analyzed using Comet assay. The particles monitoring showed that machining was the dominant source of NPs. When compared with the controls, nanocomposite-exposed workers had significantly increased levels of both DNA damage types. In subjects performing machining, post-shift DNA-SB levels were significantly higher than those pre-shift. In welders, such an effect was not observed. Importantly, in both groups, levels of oxidatively damaged DNA were comparable in the post-shift vs. pre-shift samples. The lack of increased DNA oxidation in the post-shift samples suggests the induction of adaptive processes, probably related to long-term occupational exposure to NPs, that protect the organism against this type of DNA damage. However, despite this observation, the potential negative health impacts associated with NP inhalation cannot be ruled out.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":"285-297"},"PeriodicalIF":3.1,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13520634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical compositions and chemoprotective effect of Lenzites betulinus mushroom in mononuclear cells of patients with acute coronary syndrome and heart failure in vitro.","authors":"Jovana Tubić Vukajlović, Ivan Simić, Tomislav Tosti, Katarina Djordjević, Mina Poskurica, Maja Murić, Olivera Milošević-Djordjević","doi":"10.1093/mutage/geag017","DOIUrl":"10.1093/mutage/geag017","url":null,"abstract":"<p><p>Cardiovascular diseases (CVD) are the leading cause of death globally, posing a major public health challenge due to their increasing prevalence and complex etiology. Natural products from medicinal mushrooms have gained attention for their cardioprotective properties. To evaluate whether the aqueous extract of Lenzites betulinus can protect mononuclear cells of acute coronary syndrome (ACS) and heart failure (HF) patients from therapy-induced DNA damage and to determine its polyphenolic composition. The study included 30 healthy controls, 30 ACS, and 30 HF patients. The genoprotective potential of aqueous mushroom extract at concentrations of 50, 100, 150, and 200 mg/mL was examined in vitro using the comet assay on human blood mononuclear cells. Total phenolic and flavonoid contents were determined spectrophotometrically, while polyphenolic profiling was performed using UHPLC-DAD-MS/MS. The mean level of DNA damage in treated cell cultures from healthy donors increased in a concentration-dependent manner compared with the negative control, but without statistical significance. The extract significantly reduced therapy-induced level of DNA damage in mononuclear cells of ACS and HF patients (P < .0005). DNA damage level was significantly higher in HF patients. In ACS patients, %DNA damage was significantly reduced at the three highest concentrations, while genetic damage index (GDI) decreased at all concentrations. In HF patients, %DNA damage decreased at the two highest concentrations, and GDI at all except the lowest. Phytochemical analysis revealed high levels of phenolics and flavonoids, with chlorogenic acid and quercetin as dominant compounds. L. betulinus possesses significant nutritional and functional value. Its rich phytochemical profile likely underlies its chemoprotective effects against therapy-induced DNA damage, suggesting potential as a natural agent for genome stability support in CVD patients.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":"237-248"},"PeriodicalIF":3.1,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DECREASED ADIPOSITY RESULTING FROM REDUCED FOOD INTAKE LIKELY CONTRIBUTES TO THE AMELIORATION OF GENOTOXIC ALTERATIONS IN OMEGA-3-SUPPLEMENTED CAFETERIA DIET-FED MICE.","authors":"Isadora de Oliveira Monteiro, Ângela Caroline da Luz Beretta, Marina Lummertz Magenis, João Vitor Antunes de Lima, Helen Barbosa Vicente, Otávio Lúcio Possamai, Nicollas Dos Santos da Silva, Luiza Martins Longaretti, Giulia Strapazzon, Rafaela Cristina Silva de Almeida, Adriani Paganini Damiani, Marko Gerić, Goran Gajski, Vanessa Moraes de Andrade","doi":"10.1093/mutage/geag029","DOIUrl":"10.1093/mutage/geag029","url":null,"abstract":"<p><p>Obesity is defined as the excessive accumulation of fat that can lead to the development of several diseases, such as type 2 diabetes and cardiovascular diseases. Dietary studies using animal models are essential to elucidate the mechanisms involved in obesity development. Diet-induced obesity models, including the cafeteria diet (CAF), have been employed to study obesity in rodents. Currently, research efforts have focused on treatment strategies for the metabolic dysfunctions caused by obesity. Omega-3 (O3) plays a crucial role in body development throughout life; therefore, this study aimed to evaluate the effect of O3 supplementation on biochemical and genotoxic parameters in mice that were fed a cafeteria diet. Forty-eight mice were divided into six groups (n=8/group): Standard Diet (SD); SD with O3 supplementation; CAF; CAF with O3 supplementation; CAF switched to SD; and CAF switched to SD with O3 supplementation. Animals were fed either a cafeteria or standard diet for 16 weeks and monitored for mass changes. O3 supplementation by gavage or diet switch began after the initial 16 weeks and lasted 4 weeks. At week 20, the mass was assessed again, and blood samples were collected for biochemical analyses and insulin tolerance tests. Following euthanasia, genotoxic analyses were performed. The present study demonstrated that the CAF diet led to increased food and energy intake, body mass, adiposity index, fasting blood glucose levels, insulin resistance, and genomic instability in mice. O3 supplementation partially attenuated these effects by reducing food intake, body weight, adiposity index, fasting blood glucose levels and DNA damage. The results of this study suggest that the observed improvements in metabolic and genotoxic parameters are primarily driven by reductions in energy intake and adiposity, with O3 supplementation being associated with reduced food intake under cafeteria diet conditions, highlighting its contribution to the modulation of obesity-related metabolic and genotoxic alterations.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148795769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphine modulates doxorubicin-induced genomic instability with distinct effects in osteosarcoma and fibroblast cells.","authors":"Jayne Torres de Sousa, Rafael Rodrigues Dihl, Cristina Brauwers Kubiaki, Gumercindo Pimentel-Peralta, Juliana da Silva, Ivana Grivicich, Jaqueline Nascimento Picada","doi":"10.1093/mutage/geag028","DOIUrl":"https://doi.org/10.1093/mutage/geag028","url":null,"abstract":"<p><p>Osteosarcoma is an aggressive malignant bone tumor that often requires chemotherapy with agents such as doxorubicin (Dox), which is known for its potent cytotoxic and genotoxic effects. To manage cancer-related pain, morphine is widely used due to its strong analgesic properties. The concurrent administration of these drugs in clinical practice might affect cancer therapy, since Dox is a well-established inducer of genomic instability, whereas the potential contribution of morphine to this process remains poorly understood. This study evaluated the cytotoxicity, genotoxicity, and chromosomal damage induced by morphine, both alone and in combination with Dox. Cytotoxicity was assessed in MG63 osteosarcoma cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Genotoxicity and chromosomal damage were evaluated in MG63 and non-tumor V79 fibroblast cells using the comet assay and the cytokinesis-block micronucleus (CBMN) assay. Morphine alone induced DNA damage in both MG63 and V79 cells in the comet assay and increased micronucleus frequency only in MG63 cells. In co-treatment, morphine significantly reduced Dox-induced DNA damage and chromosomal instability parameters in the CBMN assay in MG63 cells. Conversely, micronucleus frequency increased in co-treatment in V79 cells. Overall, morphine alone increased chromosomal damage in MG63 tumor cells but not in V79 non-tumor cells. However, in combination with Dox, morphine attenuated Dox-induced genomic instability in osteosarcoma cells while enhancing chromosomal damage in non-tumor fibroblasts. These findings suggest that morphine may differentially modulate the genomic effects of Dox, potentially impacting both antitumor efficacy and genomic stability in normal cells.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148685182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Geno-protective Effects of Naturally Occurring Delphinidin Glycosides.","authors":"Giorgia Martelli, Carima Lörch, Naji Said Aboud Hadi, Ezgi Eyluel Bankoglu, Norbert Roewer, Helga Stopper","doi":"10.1093/mutage/geag027","DOIUrl":"https://doi.org/10.1093/mutage/geag027","url":null,"abstract":"<p><p>Anthocyanins are flavonoid pigments responsible for red, blue, and purple coloration in plants and play a protective role against abiotic stress through reactive oxygen species scavenging. They are abundant in the human diet, with berries serving as a primary source. These flavonoids are known for their antioxidant and potential geno-protective properties. However, the effect of glycosylation on their biological activity remains incompletely understood. This study investigated the influence of glycosylation on the antioxidant and DNA-protective effects of delphinidin (aglycone), comparing the aglycone with pure glycosylated derivatives and a standardized Maqui berry extract 'PREVAID' rich in delphinidin glycosides. Using human colon adenocarcinoma (Caco-2) cells, oxidative stress was induced with hydrogen peroxide (H₂O₂) and assessed via 2',7'-dichlorodihydrofluorescein diacetate (DCFH₂-DA). Oxidative DNA damage protection was evaluated with the formamidopyrimidine DNA glycosylase (FPG)-modified comet assay, and geno-protection against cytochrome P450-mediated activation of riddelliine was assessed by the micronucleus assay in human hepatoma (HepG2) cells. Both delphinidin and its glycosylated derivatives significantly reduced intracellular ROS and protected against oxidative DNA damage. Protective effects persisted following medium replacement, indicating intracellular uptake and activity. In the micronucleus assay, delphinidin and its glycosides attenuated riddelliine-induced genotoxicity, suggesting interference with cytochrome P450-mediated metabolic activation. These findings demonstrate that glycosylation does not abolish the antioxidant and geno-protective properties of delphinidin. The results have important implications for understanding dietary anthocyanin bioactivity and guiding the development of anthocyanin-based nutraceuticals.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Industrial Genotoxicology Group: 38th Annual Meeting Report.","authors":"Grace Kocks, Fahima Begum, Peter Ceuppens, Robert S Foster, Paul Fowler, David Kirkland, Karen Philp, David J Ponting, Siret Saaremagi, Robert Smith, Benjamin P Thornton, Claudia Ventura, Abbie Williams, Amy Wilson","doi":"10.1093/mutage/geag026","DOIUrl":"https://doi.org/10.1093/mutage/geag026","url":null,"abstract":"<p><p>The 38th annual meeting of the Industrial Genotoxicology Group (IGG) was held at Lhasa Limited, Leeds, UK on 2 December 2025, and focussed on the assessment of biological relevance in genotoxicity testing and the appropriate use of historical negative control data. Presentations covered updates on assay development, practical challenges in interpreting borderline or equivocal results, and evolving regulatory expectations across OECD and International Council for Harmonisation (ICH) frameworks. The meeting also featured the New Investigator Technical Award and the Lhasa Young Scientist Memorial Award, recognising innovative technical contributions across the field. Through scientific sessions and an interactive workshop, attendees explored case studies illustrating how statistical significance, biological relevance, and quality of historical negative control (HNC) data intersect in regulatory decision-making. Key themes included the stability and interpretation of historical control data ranges, the application of statistical process control, and the complexities of assigning confidence to negative findings. The meeting highlighted the continued need for expert judgement when assessing genotoxic outcomes and the importance of transparent, high-quality data to support regulatory conclusions.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-herbal ergogenic supplement induces in vitro mutagenicity and cytogenetic damages in HepG2 and HEK293 cells.","authors":"Eduardo K Carrão Dantas, João Pedro F T Thomé, Moisés de O Pontes Filho, Marsen G P Coelho, Israel Felzenszwalb, Carlos Fernando Araujo-Lima","doi":"10.1093/mutage/geag015","DOIUrl":"10.1093/mutage/geag015","url":null,"abstract":"<p><p>Popular ergogenic supplements for weight loss raise safety concerns due to potential cardiovascular, liver, and kidney toxicity. Their genotoxicity remains insufficiently studied, highlighting the need for more research on their long-term effects. The Brazilian health surveillance and regulatory system does not include dietary supplements under OECD genotoxicity regulatory guidelines. This study evaluates the mutagenic, cytotoxic, and genotoxic effects of a multi-herbal ergogenic supplement using the Ames test, WST-1 cell viability assay, and cytokinesis-block micronucleus tests in HepG2 and HEK293 cell lines. The Ames test revealed dose-dependent mutagenicity, particularly with metabolic activation, while the WST-1 assay showed hepatocytotoxic and nephrocytotoxic potential with dose- and time-dependent response. The cytokinesis-block micronucleus assay further confirmed genotoxicity, with significant formation of micronuclei and other cellular damage indicators. Our study emphasizes the need for careful safety monitoring of ergogenic supplements, highlighting the toxicity risks associated with their mutagenic, cytotoxic, and genotoxic potential.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":"216-224"},"PeriodicalIF":3.1,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147593314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inclusion of a recovery period after pulse treatment in the primary human lymphocyte micronucleus assay covers potential cell cycle delays without loss of sensitivity.","authors":"Alina Goepfert, Miriam Spies, Natascha Partosa, Silke Spang, James Whitwell, Robert Smith, Abigail Lamb, Christine Marchand, Andreas Zeller, Jasmin Lott, Kristina Trenz, Azeddine Elhajouji, Hans-Joerg Martus, Murielle Brichet, Martin Schneider, Steffen Naumann, Markus Schulz, Jessica Dieckhoff, Naveed Honarvar, Robert Landsiedel","doi":"10.1093/mutage/geag013","DOIUrl":"10.1093/mutage/geag013","url":null,"abstract":"<p><p>According to Organisation for Economic Co-operation and Development test guideline no. (TG) 487 for the in vitro mammalian cell micronucleus test (MNT), short (pulse) treatment of primary human lymphocytes should be followed immediately by a sampling period of 1.5-2.0 cell cycle lengths in the presence of cytochalasin B. However, TG 487 allows extending the sampling period \"if it is known or suspected that the test chemical affects the cell cycling time.\" It is often unknown in advance whether a test substance induces cell cycle delays, and therefore whether an extension of the sampling period is warranted. Here, we investigate whether extending the sampling time after pulse treatment-by including a recovery period prior to cytochalasin B treatment-affects the sensitivity of the in vitro MNT. Primary human lymphocytes were exposed to 11 mutagens and assessed using two protocols (\"recovery\" and \"no recovery\") in parallel from the same treatment culture. Including a recovery period after pulse treatment generally allowed testing of higher concentrations without excessive cytotoxicity. In addition, cultures subjected to a recovery period showed generally higher micronucleus rates at concentrations with acceptable levels of cytotoxicity, compared to cultures not having undergone a recovery period. To compare the sensitivity of both protocols at non-cytotoxic concentrations, benchmark concentration analysis was performed. Here, the \"recovery method\" proved to be at least as sensitive as the \"no recovery method.\" The reproducibility of these findings was demonstrated in an interlaboratory ring trial using a subset of up to five mutagens. In conclusion, the inclusion of a recovery period after pulse treatment per se covers potential substance-induced cell cycle delays without any loss in sensitivity toward the induced mutagenic effect. The \"recovery\" protocol can be used for assessing the mutagenic potential of test substances affecting and not affecting the cell cycle.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":"189-205"},"PeriodicalIF":3.1,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrosamine Ames Data Review and Method Development: proceedings of a US FDA/HESI workshop.","authors":"Aisar Atrakchi, Raechel Puglisi, Joel Bercu, Jennifer Cheung, Andreas Czich, Roland Froestchl, Karen Davis-Bruno, Robert H Heflich, Tetyana Kobets, Timothy J McGovern, Anthony Lynch, Max Selby, Maik Schuler, Gabriela De Oliveira Silveira, Alisa Vespa, Rhys Whomsley, Connie L Chen","doi":"10.1093/mutage/geag011","DOIUrl":"10.1093/mutage/geag011","url":null,"abstract":"<p><p>Detection of N-nitrosamines (NA) in pharmaceuticals became a point of interest due to the mutagenic and carcinogenic potential of some compounds of this class, and their identification as nitrosated forms of marketed drugs, otherwise known as NA Drug Substance-Related Impurities (NDSRIs). The Ames test is used to assess the mutagenic potential of drug impurities, including NAs. Concerns over the sensitivity of the Ames test, as recommended in Organization for Economic Co-operation and Development Test Guideline 471, in predicting the rodent carcinogenic potential of NAs has prompted optimization of several test parameters used for detecting the mutagenicity of NAs (e.g. methods used for metabolic activation and the selection of tester strains). In order to discuss optimal Ames test conditions for the evaluation of NAs, including NDSRIs, the Office of New Drugs in the US Food and Drug Administration's Center for Drug Evaluation and Research and the Health and Environmental Sciences Institute's Genetic Toxicology Technical Committee co-organized and co-sponsored a workshop entitled \"Nitrosamines: Ames Data Review and Method Development Workshop\". The workshop featured five sessions addressing charge questions pertinent to the Ames test conditions and performance through the presentation of data and panel discussions. This report outlines the key takeaway points from the workshop.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":"225-236"},"PeriodicalIF":3.1,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146195109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sachi Prasad Ray-Chaudhuri: Drosophila genetics and mutagenesis in Indian science.","authors":"Rajiva Raman, Awadhesh N Jha","doi":"10.1093/mutage/geag016","DOIUrl":"10.1093/mutage/geag016","url":null,"abstract":"<p><p>This article summarizes the scientific contributions of Professor Sachi Prasad Ray-Chaudhuri and his unwavering commitment to advancing research and teaching in mutagenesis and genetics in post-independence India, following his postgraduate training in the United Kingdom under the mentorship of Professor H. J. Muller. His contributions in basic mutagenesis, radiation protection, animal cytogenetics, and population genetics using a range of model and native species laid the foundation of scientific research in India in these contemporary fields. This played a pivotal role in advancing genetics and biological sciences in India at a time when scientific infrastructure and resources were limited. He also took the initiative to modernize the curriculum of biology teaching in India in line with the developments in the subject globally. He expanded these efforts by teaching as a visiting professor in different, often remote parts of the country, where he inspired the students. The seeds of his relentless effort to connect the advances in Indian science with those in the rest of the world have blossomed, and Indian science today contributes significantly to the global knowledge base. His pioneering scientific contributions and inspiring legacy continue unabated.</p>","PeriodicalId":18889,"journal":{"name":"Mutagenesis","volume":" ","pages":"167-174"},"PeriodicalIF":3.1,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13310013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147593377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}