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Crystal structure of Bacillus subtilis glyceraldehyde-3-phosphate dehydrogenase GapB 枯草芽孢杆菌甘油醛-3-磷酸脱氢酶 GapB 的晶体结构
Korean Society for Structural Biology Pub Date : 2023-12-30 DOI: 10.34184/kssb.2023.11.4.59
P. Dahal, Deepak Pathak, Eunju Kwon
{"title":"Crystal structure of Bacillus subtilis glyceraldehyde-3-phosphate dehydrogenase GapB","authors":"P. Dahal, Deepak Pathak, Eunju Kwon","doi":"10.34184/kssb.2023.11.4.59","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.4.59","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":" 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139137734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression, purification, and crystallization of human CK2α in complex with the natural flavonoid quercetin 人CK2α与天然类黄酮槲皮素复合物的表达、纯化和结晶
Korean Society for Structural Biology Pub Date : 2023-12-27 DOI: 10.34184/kssb.2023.11.4.74
An‐Na Cho, Danbi Yoon, Jiyoung Park, Jeong Seok Cha, Jiho Yoo
{"title":"Expression, purification, and crystallization of human CK2α in complex with the natural flavonoid quercetin","authors":"An‐Na Cho, Danbi Yoon, Jiyoung Park, Jeong Seok Cha, Jiho Yoo","doi":"10.34184/kssb.2023.11.4.74","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.4.74","url":null,"abstract":"Over 300 polypeptide substrates can undergo catalysis by the constitutively active Ser/Thr kinase, human casein kinase 2 alpha (hCK2α).Quercetin, a naturally occurring polyphenolic compound, is well-known for inhibiting hCK2α, thereby regulating cellular signaling pathways.In this study, we crystallized the hCK2α-quercetin complex to elucidate the inhibitory mechanism of quercetin on hCK2α through the complex structure.The hanging drop vapor diffusion method was employed for crystallizing the hCK2α-quercetin complex, and the resulting crystal diffracted to a resolution of 2.11 Å. X-ray diffraction data analysis revealed that the crystal of the hCK2α-quercetin complex belonged to the P4 3 2 1 2 space group, with unit cell dimensions of a = b = 127.604Å, c = 124.314Å, and α = β = γ = 90.00°.In an initial density map, quercetin was bound well into hCK2α.","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"11 4","pages":"74-79"},"PeriodicalIF":0.0,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bdjn.org/journal/download_pdf.php?doi=10.34184/kssb.2023.11.4.74","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Overexpression, crystallization, and preliminary X-ray diffraction studies of NDUFA2 NDUFA2的过表达、结晶和x射线衍射初步研究
Korean Society for Structural Biology Pub Date : 2023-06-30 DOI: 10.34184/kssb.2023.11.2.28
Suk-Hyun Yoo, S. Ryu
{"title":"Overexpression, crystallization, and preliminary X-ray diffraction studies of NDUFA2","authors":"Suk-Hyun Yoo, S. Ryu","doi":"10.34184/kssb.2023.11.2.28","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.2.28","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127649752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modeling study of the HlyBD-TolC hemolysin secretion system using the AI-based structural prediction and molecular dynamics simulation 基于人工智能结构预测和分子动力学模拟的HlyBD-TolC溶血素分泌系统建模研究
Korean Society for Structural Biology Pub Date : 2023-06-30 DOI: 10.34184/kssb.2023.11.2.21
Y. Baek, Jinwook Lee, Dukwon Lee, Yongbin Xu, N. Ha
{"title":"Modeling study of the HlyBD-TolC hemolysin secretion system using the AI-based structural prediction and molecular dynamics simulation","authors":"Y. Baek, Jinwook Lee, Dukwon Lee, Yongbin Xu, N. Ha","doi":"10.34184/kssb.2023.11.2.21","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.2.21","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115827284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assembly and functional role of mitochondrial respiratory supercomplex 线粒体呼吸超复合体的组装与功能作用
Korean Society for Structural Biology Pub Date : 2023-03-30 DOI: 10.34184/kssb.2023.11.1.1
Suk-Hyun Yoo, S. Ryu
{"title":"Assembly and functional role of mitochondrial respiratory supercomplex","authors":"Suk-Hyun Yoo, S. Ryu","doi":"10.34184/kssb.2023.11.1.1","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.1.1","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131229427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-molecule fluorescence assays for studying the mechanism of DNA cleavage by CRISPR-Cas12a 用单分子荧光法研究CRISPR-Cas12a切割DNA的机制
Korean Society for Structural Biology Pub Date : 2023-03-30 DOI: 10.34184/kssb.2023.11.1.11
Yuyoung Kim, Sanghwa Lee
{"title":"Single-molecule fluorescence assays for studying the mechanism of DNA cleavage by CRISPR-Cas12a","authors":"Yuyoung Kim, Sanghwa Lee","doi":"10.34184/kssb.2023.11.1.11","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.1.11","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126901112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification, crystallization, and X-ray crystallographic analysis terephthalic acid binding protein from Ideonella sakaiensis 酒井Ideonella sakaiensis对苯二甲酸结合蛋白的纯化、结晶及x射线晶体学分析
Korean Society for Structural Biology Pub Date : 2023-03-30 DOI: 10.34184/kssb.2023.11.1.16
Seul Hoo Lee, D. Ki, Kyung-Jin Kim
{"title":"Purification, crystallization, and X-ray crystallographic analysis terephthalic acid binding protein from Ideonella sakaiensis","authors":"Seul Hoo Lee, D. Ki, Kyung-Jin Kim","doi":"10.34184/kssb.2023.11.1.16","DOIUrl":"https://doi.org/10.34184/kssb.2023.11.1.16","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129312157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryo-electron microscopy research at the institute of membrane proteins: current status and future prospects 膜蛋白研究所的低温电镜研究:现状与展望
Korean Society for Structural Biology Pub Date : 2020-12-30 DOI: 10.34184/KSSB.2020.8.4.87
Ji Won Kim, Jie-Oh Lee
{"title":"Cryo-electron microscopy research at the institute of membrane proteins: current status and future prospects","authors":"Ji Won Kim, Jie-Oh Lee","doi":"10.34184/KSSB.2020.8.4.87","DOIUrl":"https://doi.org/10.34184/KSSB.2020.8.4.87","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123383388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synchrotron serial crystallography at 11C beamline of Pohang Light Source-II 浦项光源- ii在11C光束线上的同步加速器连续晶体学
Korean Society for Structural Biology Pub Date : 2020-12-30 DOI: 10.34184/KSSB.2020.8.4.93
D. Gu, Cheolsoo Eo, Dong Tak Jeong, Jeong-Sun Kim, Suk‐Youl Park
{"title":"Synchrotron serial crystallography at 11C beamline of Pohang Light Source-II","authors":"D. Gu, Cheolsoo Eo, Dong Tak Jeong, Jeong-Sun Kim, Suk‐Youl Park","doi":"10.34184/KSSB.2020.8.4.93","DOIUrl":"https://doi.org/10.34184/KSSB.2020.8.4.93","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122373812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preliminary crystallographic study of CD38 in complex with isatuximab, an FDA-approved antibody drug for treating multiple myeloma CD38与isatuximab复合物的初步晶体学研究，isatuximab是fda批准的用于治疗多发性骨髓瘤的抗体药物
Korean Society for Structural Biology Pub Date : 2020-12-30 DOI: 10.34184/KSSB.2020.8.4.83
Hyun Tae Lee, Yujin Kim, U. Park, T. Jeong, Y. Heo
{"title":"Preliminary crystallographic study of CD38 in complex with isatuximab, an FDA-approved antibody drug for treating multiple myeloma","authors":"Hyun Tae Lee, Yujin Kim, U. Park, T. Jeong, Y. Heo","doi":"10.34184/KSSB.2020.8.4.83","DOIUrl":"https://doi.org/10.34184/KSSB.2020.8.4.83","url":null,"abstract":"","PeriodicalId":188052,"journal":{"name":"Korean Society for Structural Biology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126582711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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