Mutation research. Genetic toxicology and environmental mutagenesis最新文献_第7页

Hydrogen inhalation: in vivo rat genotoxicity tests 氢气吸入：大鼠体内遗传毒性试验

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-02-01 DOI: 10.1016/j.mrgentox.2024.503736

Cordélia Salomez-Ihl , Stéphane Tanguy , Jean-Pierre Alcaraz , Chloé Davin , Victor Pascal-Moussellard , Mariem Jabeur , Pierrick Bedouch , Ludovic Le Hegarat , Valérie Fessard , Anne-Louise Blier , Sylvie Huet , Philippe Cinquin , François Boucher

{"title":"Hydrogen inhalation: in vivo rat genotoxicity tests","authors":"Cordélia Salomez-Ihl , Stéphane Tanguy , Jean-Pierre Alcaraz , Chloé Davin , Victor Pascal-Moussellard , Mariem Jabeur , Pierrick Bedouch , Ludovic Le Hegarat , Valérie Fessard , Anne-Louise Blier , Sylvie Huet , Philippe Cinquin , François Boucher","doi":"10.1016/j.mrgentox.2024.503736","DOIUrl":"10.1016/j.mrgentox.2024.503736","url":null,"abstract":"<div>Preclinical and clinical studies have shown that molecular hydrogen (H2) has anti-oxidant, anti-inflammatory, and anti-apoptotic properties. Safety data are available in the literature and acute toxicity has been tested in isolated cells and laboratory animals. We have evaluates the genotoxicity of H2 in vivo in rats after 72 h exposure, following the International Council for Harmonization guidelines ICH S2 (R1). The study was conducted on three groups of male Wistar rats: a negative control group, a positive control group receiving methyl methanesulfonate, and a H2-treated group receiving a 3.1% H2 gas mixture for 72 h. Alkaline comet, formamidopyrimidine DNA glycosylase (Fpg)-modified comet and bone marrow micronucleus assays were performed. H2 exposure increased neither comet-tail DNA intensity (DNA damage) nor frequency of “hedgehogs” in blood, liver, lungs, or bronchoalveolar lavage fluid. No increase in Fpg-sensitive sites in lungs, no induction of micronucleus formation, and no imbalance of immature erythrocyte to total erythrocyte ratio (IME%) was observed in rats exposed to H2. The ICH S2 (R1) test-battery revealed no in vivo genotoxicity in Wistar rats after 72 h inhalation of a mixture containing 3.1% H2.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"894 ","pages":"Article 503736"},"PeriodicalIF":1.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139663457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The probable reasons of arsenic susceptibility in a chronically exposed population of West Bengal 西孟加拉邦长期接触砷的人群对砷敏感的可能原因

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-02-01 DOI: 10.1016/j.mrgentox.2024.503725

Ashok Kumar Giri , Nilanjana Banerjee

{"title":"The probable reasons of arsenic susceptibility in a chronically exposed population of West Bengal","authors":"Ashok Kumar Giri , Nilanjana Banerjee","doi":"10.1016/j.mrgentox.2024.503725","DOIUrl":"10.1016/j.mrgentox.2024.503725","url":null,"abstract":"<div>Arsenic is potent human carcinogen which affects millions of people across the globe. Arsenic induced pre-cancerous and cancerous skin lesions are hall marks of chronic arsenic toxicity. Even then, only 15%–20% of the population manifest arsenic-induced skin lesions but the rest do not, the reason for which in not very clear. Not only that, conjunctival irritations of the eyes, peripheral neuropathy and respiratory distress are the non-dermatological health effects which are often manifested in them in addition to the cancers of skin and other internal organs. In this work we have considered 233 arsenic exposed individuals with skin lesions and 205 arsenic exposed individuals without skin lesions from the highly arsenic affected Murshidabad district of West Bengal. We have compared arsenic exposure in the two groups through drinking water. Both the study groups have similar levels of arsenic exposure, drinking same arsenic laden water. Results show that higher amounts of arsenic were retained in the nails and hair of the skin lesion group compared to the no skin lesion group. Significant higher amounts of chromosomal aberration and micronucleus formation were found in the skin lesion group, than the no skin lesion group. Incidences of conjunctival irritations of the eyes, peripheral neuropathy and respiratory distress were much higher in the former group compared to the later. We, thus found that one group was more susceptible than the other, even with similar levels of arsenic exposure. We have tried to identify and discuss the probable reasons for this observation with reference to our previous works in the exposed population from West Bengal, India.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"894 ","pages":"Article 503725"},"PeriodicalIF":1.9,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139460343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persons chronically exposed to low doses of ionizing radiation: A cytogenetic dosimetry study 长期暴露于低剂量电离辐射的人：细胞遗传剂量学研究

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-01-18 DOI: 10.1016/j.mrgentox.2024.503728

Oksana Cherednichenko, Anastassiya Pilyugina, Serikbai Nuraliev, Dinara Azizbekova

{"title":"Persons chronically exposed to low doses of ionizing radiation: A cytogenetic dosimetry study","authors":"Oksana Cherednichenko, Anastassiya Pilyugina, Serikbai Nuraliev, Dinara Azizbekova","doi":"10.1016/j.mrgentox.2024.503728","DOIUrl":"10.1016/j.mrgentox.2024.503728","url":null,"abstract":"<div>The dosimetry and control of exposure for individuals chronically exposed to ionizing radiation are important and complex issues. Assessment may be optimized by evaluating individual adaptation and radiosensitivity, but it is not possible for a single model to account for all relevant parameters. Our goal was to develop approaches for the calculation of doses for persons chronically exposed to ionizing radiation, taking their radiosensitivities into consideration. On the basis of ex vivo radiation of blood samples, dose-effect models were constructed for dose ranges 0.01–2.0 and 0.01–0.4 Gy, using different cytogenetic criteria. The frequencies of \"dicentric chromosomes and rings\" at low doses are too low to have predictive value. The different responses of subjects to radiation made it possible to categorize them according to their radiosensitivities and to generate separate dose-effect curves for radiosensitive, average, and radioresistant individuals, reducing the amount of error in retrospective dosimetry.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"894 ","pages":"Article 503728"},"PeriodicalIF":1.9,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1383571824000044/pdfft?md5=86b0eba3a7285cd53331811ad0c7c585&pid=1-s2.0-S1383571824000044-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139496502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The buccal micronucleus cytome assay: New horizons for its implementation in human studies 口腔微核试验--人类研究的新视野

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-01-06 DOI: 10.1016/j.mrgentox.2023.503724

Michael Fenech , Siegfried Knasmueller , Armen Nersesyan , Claudia Bolognesi , Georg Wultsch , Christian Schunck , Emanuela Volpi , Stefano Bonassi

{"title":"The buccal micronucleus cytome assay: New horizons for its implementation in human studies","authors":"Michael Fenech , Siegfried Knasmueller , Armen Nersesyan , Claudia Bolognesi , Georg Wultsch , Christian Schunck , Emanuela Volpi , Stefano Bonassi","doi":"10.1016/j.mrgentox.2023.503724","DOIUrl":"10.1016/j.mrgentox.2023.503724","url":null,"abstract":"<div>In this report we provide a summary of the presentations and discussion of the latest knowledge regarding the buccal micronucleus (MN) cytome assay. This information was presented at the HUMN workshop held in Malaga, Spain, in connection with the 2023 European, Environmental Mutagenesis and Genomics conference. The presentations covered the most salient topics relevant to the buccal MN cytome assay including (i) the biology of the buccal mucosa, (ii) its application in human studies relating to DNA damage caused by environmental exposure to genotoxins, (iii) the association of buccal MN with cancer and a wide range of reproductive, metabolic, immunological, neurodegenerative and other age-related diseases, (iv) the impact of nutrition and lifestyle on buccal MN cytome assay biomarkers; (v) its potential for application to studies of DNA damage in children and obesity, and (vi) the growing prospects of enhancing the clinical utility by automated scoring of the buccal MN cytome assay biomarkers by image recognition software developed using artificial intelligence. The most important knowledge gap is the need of prospective studies to test whether the buccal MN cytome assay biomarkers predict health and disease.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"894 ","pages":"Article 503724"},"PeriodicalIF":1.9,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139376015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The assessment and communication of genotoxicity test results: moving beyond binary 遗传毒性测试结果的评估与交流：超越二元对立

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-01-01 DOI: 10.1016/j.mrgentox.2023.503722

Takashi Omori , Makoto Hayashi

引用次数: 0

Occupational exposure to radiation among health workers: Genome integrity and predictors of exposure 医务工作者的辐射职业暴露：基因组完整性和暴露预测因素

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-01-01 DOI: 10.1016/j.mrgentox.2024.503726

Hayal Çobanoğlu, Akın Çayır

{"title":"Occupational exposure to radiation among health workers: Genome integrity and predictors of exposure","authors":"Hayal Çobanoğlu, Akın Çayır","doi":"10.1016/j.mrgentox.2024.503726","DOIUrl":"10.1016/j.mrgentox.2024.503726","url":null,"abstract":"<div>The current study aimed to investigate genomic instabilities in healthcare workers who may experience varying levels of radiation exposure through various radiological procedures. It also sought to determine if factors related to the work environment and dosimeter reading could effectively explain the observed genomic instabilities. Utilizing the cytokinesis-block micronucleus assay (CBMN) on peripheral blood lymphocytes, we assessed a spectrum of genomic aberrations, including nucleoplasmic bridge (NPB), nuclear budding (NBUD), micronucleus (MN) formation, and total DNA damage (TDD). The study uncovered a statistically significant increase in the occurrence of distinct DNA anomalies among radiology workers (with a significance level of P < 0.0001 for all measurements). Notably, parameters such as total working hours, average work duration, and time spent in projection radiography exhibited significant correlations with MN and TDD levels in these workers. The dosimeter readings demonstrated a positive correlation with the frequency of NPB and NBUD, indicating a substantial association between radiation exposure and these two genomic anomalies. Our multivariable models identified the time spent in projection radiography as a promising parameter for explaining the overall genomic instability observed in these professionals. Thus, while dosimeters alone may not fully explain elevated total DNA damage, intrinsic work environment factors hold potential in indicating exposure levels for these individuals, providing a complementary approach to monitoring.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"893 ","pages":"Article 503726"},"PeriodicalIF":1.9,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139422527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotoxicity and the stability of N-nitrosomorpholine activity following UVA irradiation UVA辐照后n -亚硝基somorpholine活性的遗传毒性和稳定性

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-12-02 DOI: 10.1016/j.mrgentox.2023.503721

Haruna Mochizuki , Yukari Nagazawa , Sakae Arimoto-Kobayashi

{"title":"Genotoxicity and the stability of N-nitrosomorpholine activity following UVA irradiation","authors":"Haruna Mochizuki , Yukari Nagazawa , Sakae Arimoto-Kobayashi","doi":"10.1016/j.mrgentox.2023.503721","DOIUrl":"10.1016/j.mrgentox.2023.503721","url":null,"abstract":"<div>This study investigated N-nitrosomorpholine (NMOR) genotoxicity following UVA irradiation without metabolic activation. Following UVA irradiation, the photo treated NMOR (irradiated NMOR) was directly mutagenic, without UVA or metabolic activation, in the Ames test. The activity was relatively stable, and approximately 79% of the activity remained after 10 days of storage at 37 °C, 4 °C, or −20 °C. Micronuclei (MN) formation was observed in HaCaT cells after treatment with irradiated NMOR without metabolic activation. The action spectrum of MN formation in response to NMOR irradiation followed the NMOR absorption curve. In vivo, MN formation was observed in the peripheral blood reticulocytes of mice injected with irradiated NMOR under the inhibition of cytochrome P450-mediated metabolism of NMOR. Volatile NMOR may attach to environmental materials and be irradiated with environmental UVA light. Photoactivated NMOR-attached air pollutants could float in the air and fall onto the human body, leading to genotoxicity induced by the irradiated NMOR.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"893 ","pages":"Article 503721"},"PeriodicalIF":1.9,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutagenicity assessment of two potential impurities in preparations of 5-amino-2,4,6 triiodoisophthalic acid, a key intermediate in the synthesis of the iodinated contrast agent iopamidol 碘化造影剂iopamidol合成中的关键中间体- 5-氨基-2,4,6三碘二苯二甲酸制备中两种潜在杂质的致突变性评价

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-11-28 DOI: 10.1016/j.mrgentox.2023.503720

Silvia Rossi , Simona Bussi , Roberta Bonafè , Carola Incardona , Emanuela Vurro , Massimo Visigalli , Federica Buonsanti , Roberta Fretta

{"title":"Mutagenicity assessment of two potential impurities in preparations of 5-amino-2,4,6 triiodoisophthalic acid, a key intermediate in the synthesis of the iodinated contrast agent iopamidol","authors":"Silvia Rossi , Simona Bussi , Roberta Bonafè , Carola Incardona , Emanuela Vurro , Massimo Visigalli , Federica Buonsanti , Roberta Fretta","doi":"10.1016/j.mrgentox.2023.503720","DOIUrl":"https://doi.org/10.1016/j.mrgentox.2023.503720","url":null,"abstract":"<div>5-Aminoisophthalic acid and 5-nitroisophthalic acid (5-NIPA) are potential impurities in preparations of 5-amino-2,4,6-triiodoisophthalic acid, which is a key intermediate in the synthesis of the iodinated contrast agent iopamidol. We have studied their mutagenicity in silico (quantitative structure-activity relationships, QSAR) and by the bacterial reverse mutation assay (Ames test). First, the compounds were screened with the tools Derek Nexus™ and Leadscope®. Both compounds were flagged as potentially mutagenic (class 3 under ICH M7). However, contrary to the in silico prediction, neither chemical was mutagenic in the Ames test (plate incorporation method) with or without S9 metabolic activation.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"893 ","pages":"Article 503720"},"PeriodicalIF":1.9,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1383571823001389/pdfft?md5=685f5f9623b3c76ab72a40ecd22f5300&pid=1-s2.0-S1383571823001389-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138475185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative DNA damage: Induction by fructose, in vitro, and its enhancement by hydrogen peroxide DNA 氧化损伤：体外果糖的诱导作用和过氧化氢的增强作用

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-11-26 DOI: 10.1016/j.mrgentox.2023.503719

Kaoru Midorikawa , Kokoro Kobayashi , Shinya Kato , Shosuke Kawanishi , Hatasu Kobayashi , Shinji Oikawa , Mariko Murata

{"title":"Oxidative DNA damage: Induction by fructose, in vitro, and its enhancement by hydrogen peroxide","authors":"Kaoru Midorikawa , Kokoro Kobayashi , Shinya Kato , Shosuke Kawanishi , Hatasu Kobayashi , Shinji Oikawa , Mariko Murata","doi":"10.1016/j.mrgentox.2023.503719","DOIUrl":"https://doi.org/10.1016/j.mrgentox.2023.503719","url":null,"abstract":"<div>Sucrose and high-fructose corn syrup comprise nearly equal amounts of glucose and fructose. With the use of high-fructose corn syrup in the food industry, consumption of fructose, which may be a tumor promoter, has increased dramatically. We examined fructose-induced oxidative DNA damage in the presence of Cu(II), with or without the addition of H2O2. With isolated DNA, fructose induced Cu(II)-mediated DNA damage, including formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), to a greater extent than did glucose, and H2O2 enhanced the damage. In cultured human cells, 8-oxodG formation increased significantly following treatment with fructose and the H2O2-generating enzyme glucose oxidase. Fructose may play an important role in oxidative DNA damage, suggesting a possible mechanism for involvement of fructose in carcinogenesis.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"893 ","pages":"Article 503719"},"PeriodicalIF":1.9,"publicationDate":"2023-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1383571823001377/pdfft?md5=366d6e8e7fd41824a49d9c535ffb2158&pid=1-s2.0-S1383571823001377-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138489967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals 艾姆斯 MPF™ 检测法的性能评估：六种编码化学品的多中心合作研究

IF 1.9 4区医学

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-11-23 DOI: 10.1016/j.mrgentox.2023.503718

Dimitrios Spiliotopoulos , Cécile Koelbert , Marc Audebert , Ilona Barisch , Deborah Bellet , Mathilde Constans , Andreas Czich , Francis Finot , Véronique Gervais , Laure Khoury , Christian Kirchnawy , Sachiko Kitamoto , Audrey Le Tesson , Laure Malesic , Ryoko Matsuyama , Elisa Mayrhofer , Isabelle Mouche , Birgit Preikschat , Lukas Prielinger , Bernhard Rainer , Kerstin Wäse

{"title":"Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals","authors":"Dimitrios Spiliotopoulos , Cécile Koelbert , Marc Audebert , Ilona Barisch , Deborah Bellet , Mathilde Constans , Andreas Czich , Francis Finot , Véronique Gervais , Laure Khoury , Christian Kirchnawy , Sachiko Kitamoto , Audrey Le Tesson , Laure Malesic , Ryoko Matsuyama , Elisa Mayrhofer , Isabelle Mouche , Birgit Preikschat , Lukas Prielinger , Bernhard Rainer , Kerstin Wäse","doi":"10.1016/j.mrgentox.2023.503718","DOIUrl":"https://doi.org/10.1016/j.mrgentox.2023.503718","url":null,"abstract":"<div>The Ames MPF™ is a miniaturized, microplate fluctuation format of the Ames test. It is a standardized, commercially available product which can be used to assess mutagenicity in Salmonella and E. coli strains in 384-well plates using a color change-based readout. Several peer-reviewed comparisons of the Ames MPF™ to the Ames test in Petri dishes confirmed its suitability to evaluate the mutagenic potential of a variety of test items. An international multicenter study involving seven laboratories tested six coded chemicals with this assay using five bacterial strains, as recommended by the OECD test guideline 471. The data generated by the participating laboratories was in excellent agreement (93%), and the similarity of their dose response curves, as analyzed with sophisticated statistical approaches further confirmed the suitability of the Ames MPF™ assay as an alternative to the Ames test on agar plates, but with advantages with respect to significantly reduced amount of test substance and S9 requirements, speed, hands-on time and, potentially automation.</div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"893 ","pages":"Article 503718"},"PeriodicalIF":1.9,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138490976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0