S. Saito, H. Kurabayashi, K. Yamasaki, Y. Shibasaki
{"title":"Effects of Soft Laser Irradiation in Human Bone Cells and Periodontal Ligament Cells Derived from Mandible Cultured on Titanium Plates","authors":"S. Saito, H. Kurabayashi, K. Yamasaki, Y. Shibasaki","doi":"10.5984/jjpnsoclaserdent.10.2","DOIUrl":"https://doi.org/10.5984/jjpnsoclaserdent.10.2","url":null,"abstract":"The objectives of the present study were to evaluate the effects of soft laser irradiation for the proliferation and the differentiation of human bone cells and periodontal cells cultured on titanium plates and plastic dishes both derived from the identical donor.Bone cells derived from mandibular fragments and periodontal ligament (PDL) cells from mandibular first premolars were obtained simultaneously from the identical orthognathic patient. Both cells in the 6th passage were seeded (5×103 cells/well) in 24-well plastic culture dishes with or without titanium plates and incubation continued for 72 hours. During the incubation period, soft laser irradiation was performed none, once, twice, which were served as control, 1×laser, 2×laser, respectively. At the end of incubation period, both cells were used for the assays of DNA synthesis and alkaline phosphatase (ALP) activity. The following results were obtained:1. Bone cells cultured on plastic dishes did not show any significant change of DNA contents and ALP activity by the various time periods of soft laser irradiation. On the other hand, bone cells cultured on titanium plates showed significant increases of DNA contents and ALP activity by the soft laser irradiation, especially by 1×10 minutes laser irradiation group. In this group, the level of DNA contents and ALP activity by titanium-cultured-bone cells were significantly greater than those by plastic-cultured-bone cells.2. In case of PDL cells, the level of DNA contents and ALP activity were constant for any kind of groups with the exception of 2×10 minutes laser group which showed significant DNA increase as compared to control group, suggesting that the existance of titanium plates and/or the irradiation of soft laser were not effective for cultured PDL cells.These results suggest that the proliferation and the differentiation of bone cells cultured on titanium plates can be stimulated by the irradiation of soft laser in vitro.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125859485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"第31回日本レーザー歯学会講演抄録","authors":"","doi":"10.5984/jjpnsoclaserdent.30.15","DOIUrl":"https://doi.org/10.5984/jjpnsoclaserdent.30.15","url":null,"abstract":"","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122925768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CO2 Laser : Application to 4 Types of Laser Insurance Approved Cases","authors":"Kyoichi Oura","doi":"10.5984/JJPNSOCLASERDENT.31.26","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.31.26","url":null,"abstract":"","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128575128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"第32回日本レーザー歯学会講演抄録","authors":"","doi":"10.5984/jjpnsoclaserdent.30.83","DOIUrl":"https://doi.org/10.5984/jjpnsoclaserdent.30.83","url":null,"abstract":"","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"246 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124713360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kambara, M. Otsuki, K. Araki, S. Oda, Shigeyuki Nagai, T. Noguchi
{"title":"Education and problem of laser dentistry in Japanese dental school","authors":"M. Kambara, M. Otsuki, K. Araki, S. Oda, Shigeyuki Nagai, T. Noguchi","doi":"10.5984/JJPNSOCLASERDENT.24.10","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.24.10","url":null,"abstract":"","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127009347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of Er: YAG Laser to Apicoectomy","authors":"A. Ebihara, Y. Sekine, A. Takeda, H. Suda","doi":"10.5984/JJPNSOCLASERDENT.8.23","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.8.23","url":null,"abstract":"ently, many studies on the application of Er: YAG laser in the dental field have been reported. Er: YAG laser can ablate dental hard tissues with small thermal damages, and is clinically applied for removal of dental caries. However, there are few studies on apicoectomy using Er: YAG laser. The purpose of this study was to compare apicoectomy using Er: YAG laser and the conventional cutting method in vitro.Extracted single-rooted human teeth were used in this experiment. Root resection was performed with Er: YAG laser (output energy: 70mJ10pps) or a fissure bur mounted to a micro-motor. Resected root surfaces were evaluated with scanning electron microscopy.In addition, cutting efficiency of both methods and dye penetration of the cut surface were compared. After removing the tooth crown of 10 extracted single-rooted human teeth, root canals were cleaned, shaped and obturated. Then, the samples were randomly divided into two groups. Root resection by Er: YAG laser or micro-motor was perfOrtned, and working time was counted and the cut surface area measured. Then, all the root surfaces were sealed with nail polish except the resected surface. All roots were placed into 0.1% methylene blue dye for 7 days, rinsed and air-dried. Finally, they were split and the dye penetration from the resected surface was measured and statistically analyzed.The results were as follows:1. There were no smear layer or debris left on the resected surface cut by Er: YAG laser, and the shape of the cut surface was irregular. On the other hand, both smear layer and debris were observed on the surface cut by the conventional method, and the cut surface was smooth.2. The cutting efficiency of Er: YAG laser and micro-motor was 14.8 (±7.3) sec./mm2 and 4.4 (±1.7) sec./ram2, respectively. Cutting by Er: YAG laser significantly needed more time than that by micro-motor (t-test, P<0.05).3. Dye penetration length in the Er: YAG laser group and micro-motor group from the resected surface was 0.95 (±0.37) mm and 1.07 (±0.70) mm, respectively. There was no significant difference between the two groups.(J. Jpn. Soc. Laser Dent. 8: 23-30, 1997 Reprint requests to Dr. EBIHARA)","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"163 10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129227546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Ohno, A. Aoki, T. Yoshino, K. Bando, S. Oda, Hisashi Watanabe, I. Ishikawa
{"title":"The Effect of the Er: YAG Laser on the Tongue of Rat","authors":"J. Ohno, A. Aoki, T. Yoshino, K. Bando, S. Oda, Hisashi Watanabe, I. Ishikawa","doi":"10.5984/JJPNSOCLASERDENT.7.46","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.7.46","url":null,"abstract":"Clinical application of the Er: YAG Laser for dental caries and periodontal treatment has been developed. In clinical use, the effect of mis-irradiation of the Er: YAG Laser on the surrounding tissues should be considered. The purpose of this study was to examine the effect of the Er: YAG Laser on soft tissues supposing the side effect of mis-irradiation. The Er: YAG Laser was irradiated against the tongue of rat under following various conditions; the energy level: 50, 150, 300mJ/pulse, pulsing time: 1 and 10 pulses, irradiation distance: 0, 5, 10 and 20mm, and irradiated field: wet or dry. Wounds on tongue were observed macroscopically at 0, 1, 3, 7 and 14 days after laser irradiation. Some specimens were also examined by SEM. The following results were obtained: contact irradiations caused limited damages to tongue regardless of water irrigation; however, the depth of wounds depended upon the pulsing time and power level of the laser, while, non-contact irradiation showed a little or no effect against tongue. Moreover, the use of water irrigation decreased their damages effectively. With respect to wound healing, all cases were shown as normal process. Clinically, most of mis-irradiations are supposed to happen with non-contact irradiation under water spray. These results indicate that the Er: YAG Laser application for intraoral usage would be safe for the surrounding soft tissues within the usual power setting in dental treatment.(J. Jpn. Soc. Laser Dent. 7: 46-57, 1996 Reprint requests to Dr OHNO)","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121719088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transmission of Diode Laser through Dentin","authors":"M. Otsuki, M. Kijima, J. Tagami","doi":"10.5984/JJPNSOCLASERDENT.21.18","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.21.18","url":null,"abstract":": The purpose of this study was to evaluate the transmission of a diode laser through the dentin. Ten dentin disks of 1 mm in thickness were obtained from five extracted human molars. The surfaces of five disks were parallel to the long axis of the tooth, and the other five were perpendicular. The transmission of a diode laser through the dentin discs was mea-sured by a power meter. The laser transmission ratio varied between 0.049 and 0.216. The laser transmission was affected by the orientation of the dentinal tubules of the dentin disks.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125733281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Keiko Yamada, H. Kuroda, Chikao Hayashi, Natumi Yamada, K. Kamiyama
{"title":"Behaviour of Osteoclast Cultured on Laser Irradiated Dentin","authors":"Keiko Yamada, H. Kuroda, Chikao Hayashi, Natumi Yamada, K. Kamiyama","doi":"10.5984/JJPNSOCLASERDENT.5.67","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.5.67","url":null,"abstract":"Osteoclasts isolated from 3-4 days rats were cultured on laser irradiated bovineroot dentin to determine the behaviour of the osteoclasts, especially the cell adhesion as well as the resorption of dentin. Methods were as follows.. The cells were stained by FITC-marked phalloidin and observed under fluorescence microscopy.2. The cells were stained with TRAP and observed under microscopy.3. The cells were observed under SEM.Following results were obtained.Osteoclasts and resorption pits were observed on the unirradiated dentin, however, they were not seen on the laser irradiated dentin. Laser exposure to dentin seemed to inhibit the adhesion of osteoclasts.","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121763191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Takamori, H. Ohashi, J. Kato, Shigeyuki Nagai, I. Yoshida, Kyoichi Oura, A. Tsukui, Masaaki Waga, Kantaro Ohgushi, Tetsuya Amagai, J. Tagami, T. Shinoki
{"title":"Questionnaire Analysis of Risk Management of Laser Dental Treatment","authors":"K. Takamori, H. Ohashi, J. Kato, Shigeyuki Nagai, I. Yoshida, Kyoichi Oura, A. Tsukui, Masaaki Waga, Kantaro Ohgushi, Tetsuya Amagai, J. Tagami, T. Shinoki","doi":"10.5984/JJPNSOCLASERDENT.26.73","DOIUrl":"https://doi.org/10.5984/JJPNSOCLASERDENT.26.73","url":null,"abstract":"The Committee of Clinical and Safety Training held two educational seminars and two safety courses from 2013 to 2014. We conducted a questionnaire study and obtained 56 valid answers of incidents and accidents involving laser dental treatment. Most incidents and accidents were emphysema, followed by mis-irradiation, equipment failure, broken irradiation tips, and not wearing safety goggles, respectively accounting for 23, 16, 14, 13 and 11%. These results suggest that laser dental treatment requires different risk management than regular dental treatment. Additionally, it is necessary periodically to hold educational seminars and safety courses on the use of dental lasers. (J. Jpn. Soc. Laser Dent. 26:73 ~ 75, 2015 Reprint requests to Dr. SHINOKI) Key words= Dental laser, Risk management, Questionnaire, Laser safety キーワード=歯科用レーザー,医療安全管理,リスクマネジメント,アンケート調査,レーザー安全管理","PeriodicalId":187459,"journal":{"name":"Journal of Japanese Society for Laser Dentistry","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134084437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}