Molecular and Cellular Endocrinology最新文献_第10页

The mechanism of 14-3-3η in thyroxine induced mitophagy in cardiomyocytes 14-3-3η在甲状腺素诱导心肌细胞有丝分裂中的作用机制

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-05-15 DOI: 10.1016/j.mce.2024.112271

Yalan Cui , Yan Zhang , Songsong Dai , Sha Wan , Heng Guan , Decai Wang , Beifang Jin , Wenping Xiao , Fang Liu

{"title":"The mechanism of 14-3-3η in thyroxine induced mitophagy in cardiomyocytes","authors":"Yalan Cui , Yan Zhang , Songsong Dai , Sha Wan , Heng Guan , Decai Wang , Beifang Jin , Wenping Xiao , Fang Liu","doi":"10.1016/j.mce.2024.112271","DOIUrl":"10.1016/j.mce.2024.112271","url":null,"abstract":"<div><p>Hyperthyroidism is becoming increasingly important as an independent risk factor for cardiovascular disease, eventually resulting in cardiac hypertrophy and heart failure. The 14-3-3 protein family subtypes regulate many cellular processes in eukaryotes by interacting with a diverse array of client proteins. Considering that the 14-3-3η protein protects cardiomyocytes by affecting mitochondrial function, exploring the biological influence and molecular mechanisms by which 14-3-3η alleviates the cardiac hypertrophy of hyperthyroidism is imperative. In vivo and in vitro, RT-PCR, Western blot, and Mitochondrial tracking assay were performed to understand the molecular mechanism of thyroxine-induced cardiomyocyte hypertrophy. HE staining, transmission electron microscopy, and immunofluorescence were used to observe intuitively changes of hearts and cardiomyocytes. The in vivo and in vitro results indicated that overexpression of the 14-3-3η ameliorated thyroxine-induced cardiomyocyte hypertrophy, whereas knockdown of the 14-3-3η protein aggravated thyroxine-induced cardiomyocyte hypertrophy. Additionally, overexpression of the 14-3-3η protein reduces thyroxine-induced mitochondrial damage and mitophagy in cardiomyocytes. Overexpression of 14-3-3η protein improves excessive mitophagy in the myocardium caused by thyroxine and thus prevents cardiac hypertrophy.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140956552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of nitric oxide and neuroendocrine system in pain generation 一氧化氮和神经内分泌系统在疼痛产生中的作用。

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-05-14 DOI: 10.1016/j.mce.2024.112270

Aayush Gupta , Maja Vejapi , Nebojsa Nick Knezevic

引用次数: 0

Oral supplementation with resveratrol improves hormonal profile and increases expression of genes associated with thermogenesis in oophorectomy mice 口服补充白藜芦醇可改善卵巢切除小鼠的荷尔蒙状况，并增加与产热相关的基因表达。

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-05-11 DOI: 10.1016/j.mce.2024.112268

Fhelício Sampaio Viana , Juliana Andrade Pereira , Thaísa Soares Crespo , Lílian Betânia Reis Amaro , Eliezer Francisco Rocha , Alice Crespo Fereira , Deborah de Farias Lelis , Thaís de Oliveira Faria Baldo , Marcelo Perim Baldo , Sérgio Henrique Sousa Santos , João Marcus Oliveira Andrade

{"title":"Oral supplementation with resveratrol improves hormonal profile and increases expression of genes associated with thermogenesis in oophorectomy mice","authors":"Fhelício Sampaio Viana , Juliana Andrade Pereira , Thaísa Soares Crespo , Lílian Betânia Reis Amaro , Eliezer Francisco Rocha , Alice Crespo Fereira , Deborah de Farias Lelis , Thaís de Oliveira Faria Baldo , Marcelo Perim Baldo , Sérgio Henrique Sousa Santos , João Marcus Oliveira Andrade","doi":"10.1016/j.mce.2024.112268","DOIUrl":"10.1016/j.mce.2024.112268","url":null,"abstract":"<div><p>Menopause causes important bodily and metabolic changes, which favor the increased occurrence of cardiovascular diseases, obesity, diabetes, and osteoporosis. Resveratrol exerts proven effects on body metabolism, improving glucose and lipid homeostasis and reducing inflammation and oxidative stress in various organs and tissues. Accordingly, this study evaluates the effects of resveratrol supplementation on the expression of markers associated with thermogenesis in brown adipose tissue, and on the body, metabolic and hormonal parameters of female mice submitted to bilateral oophorectomy. Eighteen female mice were randomized into three groups: G1: control (CONTROL), G2: oophorectomy (OOF), and G3: oophorectomy + resveratrol (OOF + RSV); the animals were kept under treatment for twelve weeks, being fed a standard diet and treated with resveratrol via gavage. Body, biochemical, hormonal, and histological parameters were measured; in addition to the expression of markers associated with thermogenesis in brown adipose tissue. The results showed that animals supplemented with resveratrol showed reduced body weight and visceral adiposity, in addition to glucose, total cholesterol, and triglyceride levels; decreased serum FSH levels and increased estrogen levels were observed compared to the OOF group and mRNA expression of PRDM16, UCP1, and SIRT3 in brown adipose tissue. The findings of this study suggest the important role of resveratrol in terms of improving body, metabolic, and hormonal parameters, as well as modulating markers associated with thermogenesis in brown adipose tissue of female mice submitted to oophorectomy.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic impact of hypothyroidism on the functional differentiation of the mammary gland in rats 甲状腺功能减退症对大鼠乳腺功能分化的表观遗传学影响

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-05-09 DOI: 10.1016/j.mce.2024.112267

Fiorella Campo Verde Arbocco , Lourdes Inés Pascual , Daiana García , Irina Ortiz , Carlos Gamarra-Luques , Rubén Walter Carón , María Belén Hapon

引用次数: 0

The role of nitric oxide and hormone signaling in chronic stress, anxiety, depression and post-traumatic stress disorder 一氧化氮和激素信号在慢性压力、焦虑、抑郁和创伤后应激障碍中的作用。

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-05-06 DOI: 10.1016/j.mce.2024.112266

Tamara Jankovic , Marko Bogicevic , Nebojsa Nick Knezevic

引用次数: 0

Pregnancy, abortion, and birth control methods’ complicity with breast cancer occurrence 怀孕、流产和节育方法与乳腺癌发生的共谋关系。

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-05-03 DOI: 10.1016/j.mce.2024.112264

Katarzyna Rakoczy , Justyna Kaczor , Adam Sołtyk , Laura Jonderko , Mikołaj Sędzik , Julia Lizon , Anna Lewandowska , Małgorzata Saczko , Julita Kulbacka

{"title":"Pregnancy, abortion, and birth control methods’ complicity with breast cancer occurrence","authors":"Katarzyna Rakoczy , Justyna Kaczor , Adam Sołtyk , Laura Jonderko , Mikołaj Sędzik , Julia Lizon , Anna Lewandowska , Małgorzata Saczko , Julita Kulbacka","doi":"10.1016/j.mce.2024.112264","DOIUrl":"10.1016/j.mce.2024.112264","url":null,"abstract":"<div><p>Reproductive factors play significantly important roles in determining the breast cancer (BC) risk. The impact of pregnancy, abortion, and birth control methods on tumor development remains unclear. It has been found that early full-term pregnancies in young women can lower their lifetime risk of developing the type of cancer in question. However, having a first full-term pregnancy at an older age can increase this risk. The relationship between pregnancy and breast cancer (BC) is, however, much more complicated. Both induced and spontaneous abortions lead to sudden changes in hormonal balance, which could cause different effects on sensitive breast epithelial cells, making abortion a potential risk factor for breast cancer. The influence of hormonal contraception on carcinogenesis is not comprehensively understood, and therefore, more exhaustive analysis of existing data and further investigation is needed. This review explores how the mentioned reproductive factors affect the risk of breast cancer (BC), focusing on the molecular mechanisms that contribute to its complexity. By comprehending this intricate network of relationships, we can develop new strategies for predicting and treating the disease.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A brief and updated introduction to the neuroendocrine system of crustaceans 甲壳动物神经内分泌系统的最新简要介绍。

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-05-01 DOI: 10.1016/j.mce.2024.112265

Leonardo Airton Ressel Simões , Rafaella Sanfelice Normann , J. Sook Chung , Anapaula Sommer Vinagre

{"title":"A brief and updated introduction to the neuroendocrine system of crustaceans","authors":"Leonardo Airton Ressel Simões , Rafaella Sanfelice Normann , J. Sook Chung , Anapaula Sommer Vinagre","doi":"10.1016/j.mce.2024.112265","DOIUrl":"10.1016/j.mce.2024.112265","url":null,"abstract":"<div><p>The neuroendocrine system of crustaceans is complex and regulates many processes, such as development, growth, reproduction, osmoregulation, behavior, and metabolism. Once stimulated, crustaceans’ neuroendocrine tissues modulate the release of monoamines, ecdysteroids, and neuropeptides that can act as hormones or neurotransmitters. Over a few decades, research has unraveled some mechanisms governing these processes, substantially contributing to understanding crustacean physiology. More aspects of crustacean neuroendocrinology are being comprehended with molecular biology, transcriptome, and genomics analyses. Hence, these studies will also significantly enhance the ability to cultivate decapods, such as crabs and shrimps, used as human food sources. In this review, current knowledge on crustacean endocrinology is updated with new findings about crustacean hormones, focusing mainly on the main neuroendocrine organs and their hormones and the effects of these molecules regulating metabolism, growth, reproduction, and color adaptation. New evidence about vertebrate-type hormones found in crustaceans is included and discussed. Finally, this review may assist in understanding how the emerging chemicals of environmental concern can potentially impair and disrupt crustacean's endocrine functions and their physiology.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140868246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CircZNF609 inhibits miR-150-5p to promote high glucose-induced damage to retinal microvascular endothelial cells CircZNF609抑制miR-150-5p，促进高血糖诱导的视网膜微血管内皮细胞损伤。

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-04-27 DOI: 10.1016/j.mce.2024.112261

Jing Gao , Chenfei Wang , Jie Zhang , Zulifeiya Shawuti , Siyao Wang , Cunhua Ma , Juan Wang

{"title":"CircZNF609 inhibits miR-150-5p to promote high glucose-induced damage to retinal microvascular endothelial cells","authors":"Jing Gao , Chenfei Wang , Jie Zhang , Zulifeiya Shawuti , Siyao Wang , Cunhua Ma , Juan Wang","doi":"10.1016/j.mce.2024.112261","DOIUrl":"10.1016/j.mce.2024.112261","url":null,"abstract":"<div><p>Hyperglycemia is a key contributor to diabetic macrovascular and ocular complications. It triggers a cascade of cellular damage, particularly in the retinal microvascular endothelial cells (RMECs). However, the underlying molecular mechanisms remain only partially understood. This study hypothesizes that CircZNF609 plays a pivotal role in mediating high glucose-induced damage in RMECs by modulating miR-150-5p and its downstream target genes, thereby affecting cellular survival, apoptosis, and oxidative stress. Gene expression datasets (GSE193974 and GSE160308) and clinical samples were used to investigate the expression levels of CircZNF609 and its interaction with miR-150-5p in the context of diabetic retinopathy (DR). Our results demonstrate that CircZNF609 is upregulated in both peripheral blood stem cells from DR patients and high glucose-stimulated hRMECs. Functional experiments reveal that silencing CircZNF609 improves cell viability, reduces apoptosis, inhibits tube formation, and modulates oxidative stress markers, whereas CircZNF609 overexpression exacerbates these effects. Moreover, miR-150-5p, a microRNA, was found to be negatively regulated by CircZNF609 and downregulated in DR. Its overexpression mitigates high glucose-induced cell injury. Our findings suggest a novel mechanism whereby CircZNF609 exacerbates high glucose-induced endothelial cell damage by sponging miR-150-5p, implicating the CircZNF609/miR-150-5p axis as a potential therapeutic target in diabetic retinopathy.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140857056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous thyroxine increases cardiac GLUT4 translocation in insulin resistant OLETF rats 外源性甲状腺素可增加胰岛素耐受性 OLETF 大鼠心脏 GLUT4 转位

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-04-26 DOI: 10.1016/j.mce.2024.112254

Dora A. Mendez , José G. Soñanez-Organis , Xue Yang , Guillermo Vazquez-Anaya , Akira Nishiyama , Rudy M. Ortiz

{"title":"Exogenous thyroxine increases cardiac GLUT4 translocation in insulin resistant OLETF rats","authors":"Dora A. Mendez , José G. Soñanez-Organis , Xue Yang , Guillermo Vazquez-Anaya , Akira Nishiyama , Rudy M. Ortiz","doi":"10.1016/j.mce.2024.112254","DOIUrl":"10.1016/j.mce.2024.112254","url":null,"abstract":"<div><p>During insulin resistance, the heart undergoes a metabolic shift in which fatty acids (FA) account for roughly about 99% of the ATP production. This metabolic shift is indicative of impaired glucose metabolism. A shift in FA metabolism with impaired glucose tolerance can increase reactive oxygen species (ROS), lipotoxicity, and mitochondrial dysfunction, ultimately leading to cardiomyopathy. Thyroid hormones (TH) may improve the glucose intolerance by increasing glucose reabsorption and metabolism in peripheral tissues, but little is known on its effects on cardiac tissue during insulin resistance. In the present study, insulin resistant Otsuka Long Evans Tokushima Fatty (OLETF) rats were used to assess the effects of exogenous thyroxine (T4) on glucose metabolism in cardiac tissue. Rats were assigned to four groups: <strong>(1)</strong> lean, Long Evans Tokushima Otsuka (LETO; n=6), <strong>(2)</strong> LETO + T4 (8 μg/100 g BM/d × 5 wks; n = 7), <strong>(3)</strong> untreated OLETF (n = 6), and <strong>(4)</strong> OLETF + T4 (8 μg/100 g BM/d × 5 wks; n = 7). T4 increased GLUT4 gene expression by 85% in OLETF and increased GLUT4 protein translocation to the membrane by 294%. Additionally, T4 increased p-AS160 by 285%, phosphofructokinase-1 (PFK-1) mRNA, the rate limiting step in glycolysis, by 98% and hexokinase II by 64% in OLETF. T4 decreased both CPT2 mRNA and protein expression in OLETF. The results suggest that exogenous T4 has the potential to increase glucose uptake and metabolism while simultaneously reducing fatty acid transport in the heart of insulin resistant rats. Thus, L-thyroxine may have therapeutic value to help correct the impaired substrate metabolism associated with diabetic cardiomyopathy.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0303720724001102/pdfft?md5=a4a60c1819b5f93a8852ed674aad7d15&pid=1-s2.0-S0303720724001102-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140653084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FGF2 promotes the proliferation of injured granulosa cells in premature ovarian failure via Hippo-YAP signaling pathway FGF2 通过 Hippo-YAP 信号通路促进卵巢早衰中受伤颗粒细胞的增殖

IF 4.1 3区医学

Molecular and Cellular Endocrinology Pub Date : 2024-04-24 DOI: 10.1016/j.mce.2024.112248

Feiyan Cheng , Jingyuan Wang , Rongli Wang , Rumeng Pan , Zhiwei Cui , Lijun Wang , Lihui Wang , Xinyuan Yang

{"title":"FGF2 promotes the proliferation of injured granulosa cells in premature ovarian failure via Hippo-YAP signaling pathway","authors":"Feiyan Cheng , Jingyuan Wang , Rongli Wang , Rumeng Pan , Zhiwei Cui , Lijun Wang , Lihui Wang , Xinyuan Yang","doi":"10.1016/j.mce.2024.112248","DOIUrl":"10.1016/j.mce.2024.112248","url":null,"abstract":"<div><p>Young women undergoing anticancer treatment are at risk of premature ovarian failure (POF). Endometrial-derived stem cells (EnSCs) have demonstrated significant therapeutic potential for treating ovarian insufficiency, although the underlying mechanisms remain to be fully understood. This study aims to further investigate the therapeutic effects of EnSCs, particularly through the paracrine action of fibroblast growth factor 2 (FGF2), on POF. The findings show that exogenous FGF2 enhances the survival of ovarian granulosa cells damaged by cisplatin. FGF2 stimulates the proliferation of these damaged cells by suppressing the Hippo signaling pathway and activating YAP expression. In vivo experiments also revealed that FGF2 treatment significantly improves ovarian reserve and endocrine function in mice with POF. These results suggest that FGF2 can boost the proliferative capacity of damaged ovarian granulosa cells through the Hippo-YAP signaling pathway, providing a theoretical foundation for using EnSCs and FGF2 in clinical treatments for POF.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0303720724001047/pdfft?md5=6f0b2fcdcd4c4105eaa0a7fdd8fdf09f&pid=1-s2.0-S0303720724001047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140773075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0