BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)最新文献

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THE EFFECT OF THE COMBINATION OF BAY LEAVES (Syzygium polyanthum) AND BREADFRUIT LEAVES (Artocarpus altilis) ON CHOLESTEROL LEVELS IN HYPERCHOLESTEROLEMIC MICE 月桂叶和面包果叶对高胆固醇血症小鼠胆固醇水平的影响
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2025-02-05 DOI: 10.31289/biolink.v11i2.13354
Nabila Sapitri, Nanik Lestariningsih, Lilin Ika Nur Indahsari
{"title":"THE EFFECT OF THE COMBINATION OF BAY LEAVES (Syzygium polyanthum) AND BREADFRUIT LEAVES (Artocarpus altilis) ON CHOLESTEROL LEVELS IN HYPERCHOLESTEROLEMIC MICE","authors":"Nabila Sapitri, Nanik Lestariningsih, Lilin Ika Nur Indahsari","doi":"10.31289/biolink.v11i2.13354","DOIUrl":"https://doi.org/10.31289/biolink.v11i2.13354","url":null,"abstract":"Central Kalimantan has enormous natural resource potential, particularly in the area of medicinal plants such as bay leaves and breadfruit. Although, people in Central Kalimantan have utilized herbs as medicine, there has not been any scientific study done. This study aims to determine the effect of giving a combination of bay leaf and breadfruit leaf extracts in reducing cholesterol levels in Balb/c Hypercholesterolaemia mice. This study was conducted using the Mixed Method method, namely exploratory research and experimental research. The research subjects used were 28 female Balb/c mice in Hypercholesterolaemia conditions. In this study there were 7 treatments including positive control, negative control, P1 (20)%, P2 (30)%, P3 (40)%, P4 (50)%, P5 (60)% with 4 replications. The data analysis technique used in this study was One Way ANOVA. The finding of the study indicate that the combination of Bay Leaves and Breadfruit Leaves has an effect on reducing cholesterol levels in hypercholesterolemic mice, which can be seen from the p-value α (α 0.05) which is 0.000. Additionally, the most effective concentration in reducing cholesterol levels in hypercholesterolemic mice resulting in the concentration of P6 (50%) with a decrease of 60.25 Mg/dL.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"11 2","pages":"256-267"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ojs.uma.ac.id/index.php/biolink/article/download/13354/6096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147883495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
INTERACTION OF CO2 AND LIGHT AVAILABILITY ON PHOTOPHYSIOLOGY OF TROPICAL COCCOLITOPHORIDS (EMILIANIA HUXLEYI, GEPHYROCAPSA OCEANICA, AND OCHOSPHAERA SP.) co2和光有效性对热带球藻(huxleyi, gephyrocapsa oceanica和ochosphaera sp .)光生理的相互作用
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.7912
N. Rukminasari, Muhammad Lukman, Khusnul Yaqin
{"title":"INTERACTION OF CO2 AND LIGHT AVAILABILITY ON PHOTOPHYSIOLOGY OF TROPICAL COCCOLITOPHORIDS (EMILIANIA HUXLEYI, GEPHYROCAPSA OCEANICA, AND OCHOSPHAERA SP.)","authors":"N. Rukminasari, Muhammad Lukman, Khusnul Yaqin","doi":"10.31289/biolink.v9i2.7912","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.7912","url":null,"abstract":"The study to examine the calcification rate, adaptation, and the biotic response of three tropical coccolithophorids (Emiliania huxleyi, Gephyrocapsa oceanica, and Ochosphaera sp) to changes in CO2 concentration. Three selected calcifying coccolitophorids were grown at batch culture with CO2 system at two levels of CO2 (385 and 1000 ppm) and two light dark periods. The parameters measured and calculation including growth rate, particulate organic carbon content, particulate inorganic carbon content, chlorophyll a, cell size, photosynthetic, organic, inorganic carbon production, photosynthesis, and calcification rate.  The results showed that there was a different response to carbonate chemistry changes and dark and light periods in any of the analyzed parameters.  The growth rate of three selected calcifying microalgae tested was decreasing significantly at high concentrations of CO2 (1000 ppm) treatment on 14:10 hour light: dark periods. However, there was no significant difference between the two CO2 concentrations where they were illuminated by 24 hours light in growth rate.  The increasing CO2 concentration and light-dark periods were species-specific responses to photosynthesis and calcification rate for three selected calcifying microalgae.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117221942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE PRESENCE OF PLUMBUM (Pb) AND CADMIUM (Cd) HEAVY METALS OF BROILER CHICKEN 肉鸡中铅、镉重金属的存在
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.7571
Zahra Layli Rochimi, Rahayu Sri Pujiati, Prehatin Tri Rahayu Ningrum
{"title":"THE PRESENCE OF PLUMBUM (Pb) AND CADMIUM (Cd) HEAVY METALS OF BROILER CHICKEN","authors":"Zahra Layli Rochimi, Rahayu Sri Pujiati, Prehatin Tri Rahayu Ningrum","doi":"10.31289/biolink.v9i2.7571","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.7571","url":null,"abstract":"The level of meat production from purebred chickens in East Java Province from 2009 to 2019 reached 510,535.29 tons. The existence of heavy metal contamination in chickens are dangerous for human health due to the lack of good facilities and infrastructure on broiler farms including land and locations that must be environmentally friendly, and when rearing chickens in paying attention to the needs of water and feed for broiler chickens that are not polluted by heavy metals. The objective of this study was to describe the levels of heavy metals in chicken meat at X Chicken Farm in Kertosari Village, Pakusari District, Jember Regency. This research was a type of descriptive research. The subjects of this study were 20 samples of broiler chicken, well water, and 4 respondents. The sampling technique used purposive sampling. Data analysis used descriptive to describe the use of chicken feed, sources of drinking water, and the presence of heavy metal levels of Pb and Cd in chicken. The result indicate that as many as 20 samples of chicken meat contained levels of heavy metals Pb and Cd below the quality standard values according to Indonesian Nasional Standard (SNI) 7387:2009), namely (pb 0.3/kg) and (Cd <0.0200) while for water drinking water used comes from drilled wells containing Pb 0.001 mg/l and Cd 0.001 mg/l..\u0000 ","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129117093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
QUALITATIVE ANALYSIS OF SALAK LEAF EXTRACT (SALACCA SUMATRANA) 腊腊鱼叶提取物的定性分析
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.8219
Industri Kesehatan Jurnal Biologi Lingkungan, Jalilah Azizah Lubis, Sahlan Tuah Harahap, Fatma, Suryani Harahap
{"title":"QUALITATIVE ANALYSIS OF SALAK LEAF EXTRACT (SALACCA SUMATRANA)","authors":"Industri Kesehatan Jurnal Biologi Lingkungan, Jalilah Azizah Lubis, Sahlan Tuah Harahap, Fatma, Suryani Harahap","doi":"10.31289/biolink.v9i2.8219","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.8219","url":null,"abstract":"Qualitative analysis research of salak leaf extract has been carried out, where the salak leaf extract Padangsidimpuan has characteristic with a larger size and does leaf has a meat which has a distinctive sour taste, because of its acid content, it is processed in the form of salak leaf (Salacca sumatrana) which aims to analyze the levels of secondary metabolites in salak leaf which are expected to be a cholesterol-lowering herbal medicine. With the appropriate qualitative analysis of salak leaf using the extraction method, namely the maceration method with methanol solvent, the results obtained that salak leaf contains Secondary metabolites consisting of alkaloids, phenolics, saponins, tannins, terpenoids, however, it does not contain flavonoids and steroids.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130563667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CARBON STORAGE IN SEAGRASS BEDS IN THE LITORAL ZONE OF SANCANG BEACH, GARUT 三塘滩涂滨海带海草床碳储量研究
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.7778
D. Koswara, D. Hernawati, D. M. Chaidir
{"title":"CARBON STORAGE IN SEAGRASS BEDS IN THE LITORAL ZONE OF SANCANG BEACH, GARUT","authors":"D. Koswara, D. Hernawati, D. M. Chaidir","doi":"10.31289/biolink.v9i2.7778","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.7778","url":null,"abstract":"The vast expanse of seagrass beds in coastal areas can provide many ecosystem services, one of which is the ability of seagrass beds to absorb and store carbon dioxide (CO2). Carbon dioxide gas (CO2) is one of the components of greenhouse gases that contribute to global warming. So the role of seagrass is very important in the ecosystem. This study aims to determine carbon storage in seagrass in the littoral zone of Sancang Beach, Garut Regency, which was carried out in April-June 2022. Determination of potential carbon storage was carried out by taking seagrass samples with a size of 20 cm x 20 cm, then drying to a constant weight. and calculated using a carbon conversion factor for seagrass biomass of 0.34. The results showed that the carbon content at Station 1 Cibako (145.9331 g C/m2) was higher than Station 2 Cikujangjambe (110.026 g C/m2) and Station 3 Ciporeang (117.771 g C/m2). The average carbon content at Sancang beach is 124,577 g C/m2. The conclusion is that in general the carbon storage below the substrate (below ground) in seagrass in the Litoral Zone of Sancang Beach has a greater value than the value of carbon storage above the substrate (above ground).","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130222888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SOIL QUALITY IN ORGANIC AGRICULTURAL LAND: STUDY OF CHEMICAL ANALYSIS AND SOIL MICROBIOLOGY 有机农用地土壤质量:化学分析与土壤微生物学研究
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.8178
Melfa Aisyah Hutasuhut, Husnarika Febriani, Leni Widiarti
{"title":"SOIL QUALITY IN ORGANIC AGRICULTURAL LAND: STUDY OF CHEMICAL ANALYSIS AND SOIL MICROBIOLOGY","authors":"Melfa Aisyah Hutasuhut, Husnarika Febriani, Leni Widiarti","doi":"10.31289/biolink.v9i2.8178","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.8178","url":null,"abstract":"Increased public awareness for a healthy diet must be balanced with successful cultivation. Organic farming system is the right choice since it leaves all non-organic components. This study aims to identify the chemical and microbiological properties of agricultural soils that apply organic systems located in Batang Buluh Village, Pematang Johar, Deli Serdang Regency, North Sumatra. Chemical analysis was carried out at Socfindo Laboratory in Medan, including testing the pH of H2O, total P and K, C Organic, N Kjehldahl, and CEC (Cation Exchange Capacity). Soil microbiological tests were carried out at Medan Regional Health Lab including gram staining tests and biochemical tests. The results of chemical analysis from the analysis of pH H2O, P and total K, Organic C, N Kjehldahl, and CEC (Cation Exchange Capacity) at the edges and middle each obtained that was pH 5 -6, soil total P content was 0.0260% up to 0.450%, available K analysis, namely 0.200% and 0.210%, organic C content obtained results of 0.970% and 0.630%, N content using the KJehldahl method was 0.150% and 0.090%, and CEC obtained results of 14.330 me/100 and 10.090 me/100 g. Related species of Bacillus contained in the soil were Gram-positive, rod-shaped, spore-forming, aerobic or facultative anaerobic, motile bacteria with peritrichous flagella.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121739136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UTILIZATION OF EUCALYPTUS GRANDIS LEAF WASTE IN THE PRODUCTION OF VCO BASED LIQUID SOAP 大桉叶废弃物在vco基液体皂生产中的利用
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.8535
Desi Ardilla, Khairunnisa Rangkuti, M. Taufik
{"title":"UTILIZATION OF EUCALYPTUS GRANDIS LEAF WASTE IN THE PRODUCTION OF VCO BASED LIQUID SOAP","authors":"Desi Ardilla, Khairunnisa Rangkuti, M. Taufik","doi":"10.31289/biolink.v9i2.8535","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.8535","url":null,"abstract":"Eucalyptus grandis  still not much used, especially the leaves. Besides being used as an industrial material, Eucalyptus is also used as a medicinal plant to cure infectious diseases, fever and rheumatism as well as being an antiseptic and can be applied in the form of bath soap. The purpose of this study was to determine the best combination of liquid body soap with the addition of eucalyptus leaf extract with various concentrations. The research method used was a laboratory experiment using a completely randomized design (CRD) method with the addition of eucalyptus oil A = 1% (w/w), B = 2% (w/w), C = 3% (w/w), D = 4% (w/w) and E = 5% in total VCO 80 oil, 40% KOH = 35 g, glycerin = 60 g, propyleneglycol = 35 g, three times the repetition. The parameters observed were the physicochemical properties of soap based on SNI Liquid Bath Soap 06-4085-1996 including free alkali content, namely A = 0.072%, B = 0.052%, C = 0.078%, D = 0.090$, E-), 104% , pH value, A= 8.36, B= 8.78, C= 8.80, D= 8.85 and E=8.94, Specific Gravity A= 1.008, B= 1.018, C= 1.009, D= 1.190, E = 1.014.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132799529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE EFFECT OF WEED MANAGEMENT USING HERBICIDE ON CORN RESULTS (Zea mays L) 除草剂杂草管理对玉米产量的影响(Zea mays L)
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.8569
Fitria, Nana Trisna Mei Br Kabeakan, Nurhajijah
{"title":"THE EFFECT OF WEED MANAGEMENT USING HERBICIDE ON CORN RESULTS (Zea mays L)","authors":"Fitria, Nana Trisna Mei Br Kabeakan, Nurhajijah","doi":"10.31289/biolink.v9i2.8569","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.8569","url":null,"abstract":"Weeds are undesired plants to grow so weed management is expected to overcome losses caused by weeds. One way to manage weeds using herbicides is the chemicals used for weed management in plants. This study aims to determine the influence of weed management using herbicides on corn yields. The research was conducted in Sekoci Village, Besitang District, Langkat Regency, North Sumatra in April-June 2021. This study used a non-factorial Randomized Block Design (RBD), with 4 treatments and 3 replications, namely contact, systemic, weeding and weeds without management. The results showed that herbicide management significantly affected 100 seed weight, cob weight per plot and seed weight per plot, but had no significant effect on weed without management. It indicates that the treatment of weed management using herbicides affects plant growth and yield. Weed management for farmers is very important to prevent competition for nutrients between weeds and corn.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122903578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
ANALYSIS OF FORMULATION MEDIA FOR PLANTING OYSTER MUSHROOM (Pleurotus ostreatus)AGRICULTURAL WASTE BASED MATERIALS 种植平菇(Pleurotus ostreatus)农业废弃物原料配方培养基分析
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.7504
S. Mardiana, E. L. Panggabean, Indah Apriliya, M. Usman
{"title":"ANALYSIS OF FORMULATION MEDIA FOR PLANTING OYSTER MUSHROOM (Pleurotus ostreatus)AGRICULTURAL WASTE BASED MATERIALS","authors":"S. Mardiana, E. L. Panggabean, Indah Apriliya, M. Usman","doi":"10.31289/biolink.v9i2.7504","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.7504","url":null,"abstract":"The effort of Indonesian people to fulfill their protein intake in an economically accessible way is by producing and consuming oyster mushrooms, because their protein content was viable as an alternative to animal-based products. Combination of sugarcane bagasse, palm fronds (PKS), and sawdust can be used as growing medium for oyster mushrooms. The aim of this study was: (1) to analyze the change in the pH of the combination media formulation of Palm Oil Frond Waste (PKS), sugarcane bagasse, and sawdust; (2) identifying the composition of nutrients in the aforementioned agricultural waste as a growing medium for oyster mushroom. This study was composed of two steps: 1) Preparing the composition of the growing medium from agricultural waste; and 2) Analyzing nutrient composition of the growing medium of oyster mushroom. The research was conducted by using Analysis of Variance (ANOVA) with a Non-Factorial Completely Randomized Design (RAL). The combination of oyster mushroom growing medium from palm fronds, sugarcane bagasse, and sawdust has different pH levels and nutritional contents. The combination of M0 – M8 oyster mushroom growing medium has the suitable level of pH to grow the mushrooms at 6,5 – 8,0 pH. From the formulation analysis of nutrient in the combination of M0 – M8, it can be used as a reference for the nutrient content of oyster mushrooms growing medium from palm fronds and sugarcane bagasse.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125632677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ANALYSIS OF NEW PRIMER PAIR CANDIDATES OF rbcL GENE FOR IDENTIFICATION OF MICROALGAE SCENEDESMACEAE 微藻科rbcL基因新引物候选对分析
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Pub Date : 2023-02-15 DOI: 10.31289/biolink.v9i2.7918
M. R. Pikoli
{"title":"ANALYSIS OF NEW PRIMER PAIR CANDIDATES OF rbcL GENE FOR IDENTIFICATION OF MICROALGAE SCENEDESMACEAE","authors":"M. R. Pikoli","doi":"10.31289/biolink.v9i2.7918","DOIUrl":"https://doi.org/10.31289/biolink.v9i2.7918","url":null,"abstract":"Scenedesmaceae is one of the microalgae groups that has been widely studied as promising biodiesel feedstock. Its morphological identification is often confused by environmental changes, so it requires molecular identification as well. The current study aimed to obtain primer pair candidates that identify the Scenedesmaceae based on the rbcL gene. The research used bioinformatics tools, which harvested rbcL protein sequence data, performed multiple sequence alignments, and designed primers based on conserved and less-conserved regions. The best left and right primers selected based on sequence length, melting temperature, 3' end stability, number of hairpins, and self-dimers, were paired, and three candidates were obtained. The three pairs were examined based on melting temperature difference, number of hetero-dimers, length of amplified nucleotide product, number of hits, and number of genera captured from the GenBank. Sce-16 (F, 5'-TGGTCGTGCTGTTTATGAATGT-3' and 1_RL, 5'-TGCCAAACATGAATACCACCA-3'), which is back-translated according to Hariotina sp. (AOY36008.1), is the most preferred candidate compared to the other two pairs after discussing their advantages and disadvantages. In the future, the proposed primer candidate needs to be validated through in vitro amplification with some optimizations to eliminate potential weaknesses.","PeriodicalId":186452,"journal":{"name":"BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan)","volume":"23 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126320747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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